Journal of Biomedical Science and Engineering最新文献_第3页

Hypoxia promotes growth of stem cells in dental follicle cell populations. 缺氧促进牙滤泡细胞群干细胞的生长。

Journal of Biomedical Science and Engineering Pub Date : 2011-01-01 Epub Date: 2011-06-01 DOI: 10.4236/jbise.2011.46057

Yuntao Dai, Hongzhi He, Gary E Wise, Shaomian Yao

{"title":"Hypoxia promotes growth of stem cells in dental follicle cell populations.","authors":"Yuntao Dai, Hongzhi He, Gary E Wise, Shaomian Yao","doi":"10.4236/jbise.2011.46057","DOIUrl":"https://doi.org/10.4236/jbise.2011.46057","url":null,"abstract":"Adult stem cells (ASC) have been found in many tissues and are of great therapeutic potential due to their capability of differentiation. However, ASC comprise only a small fraction of the tissues. In order to use ASC for therapeutic purposes, it is important to obtain relatively pure stem cells in large quantities. Current methods for stem cell purification are mainly based on marker-dependent cell sorting techniques, which have various technical difficulties. In this study, we have attempted to develop novel conditions to favor the growth of the dental follicle stem cells (DFSC) such that the resultant cell populations are enriched in stem cells. Specifically, a heterogeneous dental follicle cell (H-DFC) population containing stem cells and homogenous non-stem cell dental follicle cell population were cultured at 1% or 5% hypoxic conditions. Only the heterogeneous population could increase proliferation in the hypoxic condition whereas the homogenous DFC did not change their proliferation rate. In addition, when the resultant cells from the heterogonous population were subjected to differentiation, they appeared to have a higher capacity of adipogenesis and osteogenesis as compared to the controls grown in the normal atmosphere (normoxic condition). These hypoxia-treated cells also express higher levels of some stem cell markers. Together, these data suggest that stem cells are enriched by culturing the heterogeneous cell populations in a reduced O(2) condition.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"4 6","pages":"454-461"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327300/pdf/nihms329677.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30584820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Chondrogenic differentiation of stem cells in human umbilical cord stroma with PGA and PLLA scaffolds. PGA和PLLA支架对人脐带间质干细胞成软骨分化的影响。

Journal of Biomedical Science and Engineering Pub Date : 2010-11-01 DOI: 10.4236/jbise.2010.311135

Liang Zhao, Michael S Detamore

{"title":"Chondrogenic differentiation of stem cells in human umbilical cord stroma with PGA and PLLA scaffolds.","authors":"Liang Zhao, Michael S Detamore","doi":"10.4236/jbise.2010.311135","DOIUrl":"https://doi.org/10.4236/jbise.2010.311135","url":null,"abstract":"The stem cells in the umbilical cord stroma, or Wharton's jelly, are referred to as human umbilical cord mesenchymal stromal cells (hUCMSCs) and have been shown to differentiate along a chondrogenic lineage. The aim of this study was to evaluate the chondrogenic differentiation of hUCMSCs in either polyglycolic acid (PGA) or poly-L-lactic acid (PLLA) non-woven mesh scaffolds for cartilage tissue engineering. PGA is widely known to degrade faster than PLLA, and over longer time scales, and differences may be expected to emerge after extended culture periods. Therefore, the focus of this study was to evaluate differences over a shorter duration. After 21 days of culture in PLLA or PGA scaffolds, hUCMSC constructs were analyzed for biochemical content, histology, and gene expression. Overall, there were only minute differences between the two scaffold groups, with similar gene expression and biosynthesis. The most notable difference was a change in shape from cylindrical to spherical by the PGA, but not PLLA, scaffold group. The overall similar behavior of the groups may suggest that in vivo application of hUCMSC-seeded PLLA or PGA scaffolds, following a 21-day pre-culture period, may yield similar constructs at the time of implantation. However, differences may begin to become more apparent with in vivo performance following implantation, or with in vitro performance over longer time periods.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 11","pages":"1041-1049"},"PeriodicalIF":0.0,"publicationDate":"2010-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4236/jbise.2010.311135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33408929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Global pattern of pairwise relationship in genetic network. 基因网络中成对关系的总体模式。

Journal of Biomedical Science and Engineering Pub Date : 2010-10-01 DOI: 10.4236/jbise.2010.310128

Ao Yuan, Qingqi Yue, Victor Apprey, George E Bonney

{"title":"Global pattern of pairwise relationship in genetic network.","authors":"Ao Yuan, Qingqi Yue, Victor Apprey, George E Bonney","doi":"10.4236/jbise.2010.310128","DOIUrl":"10.4236/jbise.2010.310128","url":null,"abstract":"In recent times genetic network analysis has been found to be useful in the study of gene-gene interactions, and the study of gene-gene correlations is a special analysis of the network. There are many methods for this goal. Most of the existing methods model the relationship between each gene and the set of genes under study. These methods work well in applications, but there are often issues such as non-uniqueness of solution and/or computational difficulties, and interpretation of results. Here we study this problem from a different point of view: given a measure of pair wise gene-gene relationship, we use the technique of pattern image restoration to infer the optimal network pair wise relationships. In this method, the solution always exists and is unique, and the results are easy to interpret in the global sense and are computationally simple. The regulatory relationships among the genes are inferred according to the principle that neighboring genes tend to share some common features. The network is updated iteratively until convergence, each iteration monotonously reduces entropy and variance of the network, so the limit network represents the clearest picture of the regulatory relationships among the genes provided by the data and recoverable by the model. The method is illustrated with a simulated data and applied to real data sets.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 10","pages":"977-985"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144784/pdf/nihms-272840.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30044876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Granger-Geweke causality model to evaluate the effective connectivity of primary motor cortex (M1), supplementary motor area (SMA) and cerebellum. 运用Granger-Geweke因果关系模型评价初级运动皮质(M1)、辅助运动区(SMA)和小脑的有效连通性。

Journal of Biomedical Science and Engineering Pub Date : 2010-09-01 DOI: 10.4236/jbise.2010.39115

Le Zhang, Guangjin Zhong, Yukun Wu, Mark G Vangel, Beini Jiang, Jian Kong

{"title":"Using Granger-Geweke causality model to evaluate the effective connectivity of primary motor cortex (M1), supplementary motor area (SMA) and cerebellum.","authors":"Le Zhang, Guangjin Zhong, Yukun Wu, Mark G Vangel, Beini Jiang, Jian Kong","doi":"10.4236/jbise.2010.39115","DOIUrl":"https://doi.org/10.4236/jbise.2010.39115","url":null,"abstract":"Currently, Granger-Geweke causality models have been widely applied to investigate the dynamic direction relationships among brain regions. In a previous study, we have found that the right hand finger-tapping task can produce relatively reliable brain response. As an extension of our previous study, we developed an algorithm based on the classical Granger-Geweke causality model to further investigate the effective connectivity of three brain regions (left primary motor cortex (M1), supplementary motor area (SMA) and right cerebellum) that showed the most robust brain activations. Our computational results not only confirm the strong linear feedback among SMA, M1 and right cerebellum, but also demonstrate that M1 is the hub of these three regions indicated by the anatomy research. Moreover, the model predicts the high intermediate node density existing in the area between SMA and M1, which will stimulate the imaging experimentalists to carry out new experiments to validate this postulation.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 ","pages":"848-860"},"PeriodicalIF":0.0,"publicationDate":"2010-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4236/jbise.2010.39115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29497772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

An Analysis of Quantitative PCR Reliability Through Replicates Using the C Method. 用C法进行重复定量PCR可靠性分析。

Journal of Biomedical Science and Engineering Pub Date : 2010-05-01 DOI: 10.4236/jbise.2010.35064

Chris C Stowers, Frederick R Haselton, Erik M Boczko

{"title":"An Analysis of Quantitative PCR Reliability Through Replicates Using the C Method.","authors":"Chris C Stowers, Frederick R Haselton, Erik M Boczko","doi":"10.4236/jbise.2010.35064","DOIUrl":"https://doi.org/10.4236/jbise.2010.35064","url":null,"abstract":"There is considerable interest in quantitatively measuring nucleic acids from single cells to small populations. The most commonly employed laboratory method is the real-time polymerase chain reaction (PCR) analyzed with the crossing point or crossing threshold (C(t)) method. Utilizing a multiwell plate reader we have performed hundreds of replicate reactions at each of a set of initial conditions whose initial number of copies span a concentration range of ten orders of magnitude. The resultant C(t) value distributions are analyzed with standard and novel statistical techniques to assess the variability/reliability of the PCR process. Our analysis supports the following conclusions. Given sufficient replicates, the mean and/or median C(t) values are statistically distinguishable and can be rank ordered across ten orders of magnitude in initial template concentration. As expected, the variances in the C(t) distributions grow as the number of initial copies declines to 1. We demonstrate that these variances are large enough to confound quantitative classification of the initial condition at low template concentrations. The data indicate that a misclassification transition is centered around 3000 initial copies of template DNA and that the transition region correlates with independent data on the thermal wear of the TAQ polymerase enzyme. We provide data that indicate that an alternative endpoint detection strategy based on the theory of well mixing and plate filling statistics is accurate below the misclassification transition where the real time method becomes unreliable.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 5","pages":"459-469"},"PeriodicalIF":0.0,"publicationDate":"2010-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902203/pdf/nihms190279.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29129146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Applications of a new In vivo tumor spheroid based shell-less chorioallantoic membrane 3-D model in bioengineering research. 基于活体肿瘤球体的无壳绒毛膜尿囊膜三维模型在生物工程研究中的应用。

Journal of Biomedical Science and Engineering Pub Date : 2010-01-01 DOI: 10.4236/jbise.2010.31003

Nzola De Magalhães, Lih-Huei L Liaw, Michael Berns, Vittorio Cristini, Zhongping Chen, Dwayne Stupack, John Lowengrub

{"title":"Applications of a new In vivo tumor spheroid based shell-less chorioallantoic membrane 3-D model in bioengineering research.","authors":"Nzola De Magalhães, Lih-Huei L Liaw, Michael Berns, Vittorio Cristini, Zhongping Chen, Dwayne Stupack, John Lowengrub","doi":"10.4236/jbise.2010.31003","DOIUrl":"https://doi.org/10.4236/jbise.2010.31003","url":null,"abstract":"The chicken chorioallantoic membrane (CAM) is a classical in vivo biological model in studies of angiogenesis. Combined with the right tumor system and experimental configuration this classical model can offer new approaches to investigating tumor processes. The increase in development of biotechnological devices for cancer diagnosis and treatment, calls for more sophisticated tumor models that can easily adapt to the technology, and provide a more accurate, stable and consistent platform for rapid quantitative and qualitative analysis. As we discuss a variety of applications of this novel in vivo tumor spheroid based shell-less CAM model in biomedical engineering research, we will show that it is extremely versatile and easily adaptable to an array of biomedical applications. The model is particularly useful in quantitative studies of the progression of avascular tumors into vascularized tumors in the CAM. Its environment is more stable, flat and has a large working area and wider field of view excellent for imaging and longitudinal studies. Finally, rapid data acquisition, screening and validation of biomedical devices and therapeutics are possible with the short experimental window.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 1","pages":"20-26"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019609/pdf/nihms254817.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29606661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Phosphatidylinositol transfer proteins: sequence motifs in structural and evolutionary analyses. 磷脂酰肌醇转移蛋白:结构和进化分析中的序列基序。

Journal of Biomedical Science and Engineering Pub Date : 2010-01-01 Epub Date: 2010-01-12 DOI: 10.4236/jbise.2010.31010

Gerald J Wyckoff, Ada Solidar, Marilyn D Yoden

{"title":"Phosphatidylinositol transfer proteins: sequence motifs in structural and evolutionary analyses.","authors":"Gerald J Wyckoff, Ada Solidar, Marilyn D Yoden","doi":"10.4236/jbise.2010.31010","DOIUrl":"https://doi.org/10.4236/jbise.2010.31010","url":null,"abstract":"Phosphatidylinositol transfer proteins (PITP) are a family of monomeric proteins that bind and transfer phosphatidylinositol and phosphatidylcholine between membrane compartments. They are required for production of inositol and diacylglycerol second messengers, and are found in most metazoan organisms. While PITPs are known to carry out crucial cell-signaling roles in many organisms, the structure, function and evolution of the majority of family members remains unexplored; primarily because the ubiquity and diversity of the family thwarts traditional methods of global alignment. To surmount this obstacle, we instead took a novel approach, using MEME and a parsimony-based analysis to create a cladogram of conserved sequence motifs in 56 PITP family proteins from 26 species. In keeping with previous functional annotations, three clades were supported within our evolutionary analysis; two classes of soluble proteins and a class of membrane-associated proteins. By, focusing on conserved regions, the analysis allowed for in depth queries regarding possible functional roles of PITP proteins in both intra- and extra- cellular signaling.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 1","pages":"65-77"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4943071/pdf/nihms-792291.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34680050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A 4-channel 3 Tesla phased array receive coil for awake rhesus monkey fMRI and diffusion MRI experiments. 4通道3特斯拉相控阵接收线圈用于清醒猕猴功能磁共振成像和扩散MRI实验。

Journal of Biomedical Science and Engineering Pub Date : 2010-01-01 DOI: 10.4236/jbise.2010.311141

Mark Haig Khachaturian

{"title":"A 4-channel 3 Tesla phased array receive coil for awake rhesus monkey fMRI and diffusion MRI experiments.","authors":"Mark Haig Khachaturian","doi":"10.4236/jbise.2010.311141","DOIUrl":"https://doi.org/10.4236/jbise.2010.311141","url":null,"abstract":"Awake monkey fMRI and diffusion MRI combined with conventional neuroscience techniques has the potential to study the structural and functional neural network. The majority of monkey fMRI and diffusion MRI experiments are performed with single coils which suffer from severe EPI distortions which limit resolution. By constructing phased array coils for monkey MRI studies, gains in SNR and anatomical accuracy (i.e., reduction of EPI distortions) can be achieved using parallel imaging. The major challenges associated with constructing phased array coils for monkeys are the variation in head size and space constraints. Here, we apply phased array technology to a 4-channel phased array coil capable of improving the resolution and image quality of full brain awake monkey fMRI and diffusion MRI experiments. The phased array coil is that can adapt to different rhesus monkey head sizes (ages 4-8) and fits in the limited space provided by monkey stereotactic equipment and provides SNR gains in primary visual cortex and anatomical accuracy in conjunction with parallel imaging and improves resolution in fMRI experiments by a factor of 2 (1.25 mm to 1.0 mm isotropic) and diffusion MRI experiments by a factor of 4 (1.5 mm to 0.9 mm isotropic).","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"3 11","pages":"1085-1092"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019607/pdf/nihms256531.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29606658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Efficiency of diagnostic model to predict recurrent suicidal incidents in diverse world communities. 诊断模型在不同世界社区预测复发性自杀事件的效率。

Journal of Biomedical Science and Engineering Pub Date : 2009-11-01 DOI: 10.4236/jbise.2009.27074

Vatsalya Vatsalya, Kan Chandras, Shweta Srivastava, Robert Karch

{"title":"Efficiency of diagnostic model to predict recurrent suicidal incidents in diverse world communities.","authors":"Vatsalya Vatsalya, Kan Chandras, Shweta Srivastava, Robert Karch","doi":"10.4236/jbise.2009.27074","DOIUrl":"https://doi.org/10.4236/jbise.2009.27074","url":null,"abstract":"Suicidal attempts have a very significant effect on the society, and they also reflect on the efforts of the supporting health care and counseling facilities; and the mental health professionals involved. The impact of suicide is further magnified by the needs of persons who attempt suicide multiple times, requiring emergency health care and rehabilitation. Preventing such activities becomes a major task for the support providing agencies as soon as patient with such tendencies are identified. There are repetitive traits that can be observed during the entire therapeutic program among the high-risk group individuals, who are susceptible to this kind of activity and such traits indicate for specific profiling. The aim of the instrument is to prevent the occurrence of the repetitive suicidal attempts of the patients in various world regions, which may have significantly higher and concerning suicide rates. This profile has been constructed on the various parameters recognized in the statistical analysis of the patient population, which have been identified or can be under treatment for their suicidal behavior. This instrument is developed to predict the probability of population segments who may attempt suicide and repetitively, by matching the parameters of the profile with that of the patient pool. Building a profile for the purpose of predicting behavior of this kind can strengthen the intervention strategies more comprehensively and reduce such incidents and health care requirements and expenses.","PeriodicalId":15173,"journal":{"name":"Journal of Biomedical Science and Engineering","volume":"2 7","pages":"516-520"},"PeriodicalIF":0.0,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164970/pdf/nihms626558.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32680646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0