Journal of Biotechnology Research Center最新文献

{"title":"Review Article: DNA Methylation in Cancer Immunity","authors":"Istikrar M. Hade, Yasir B. Qaddoori, Rakad M. Kh AL-Jumaily","doi":"10.24126/jobrc.2024.18.1.806","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.806","url":null,"abstract":"Background: The transcriptional control of various cell types, especially in the development or functioning of immune system cells involved in either promoting or inhibiting the immune response against cancer, is significantly influenced by DNA or RNA methylation. Multifaceted interconnections exist between immunological or cancer cell populations in the tumor's microenvironment (TME). TME alters the fluctuating DNA (as well as RNA) methylation sequences in these immunological cells to change their development into pro- or anti-cancer cell categories (such as T cells, which are regulatory, for instance). Objective: This review highlights the impact of DNA and RNA methylation on myeloid and lymphoid cells, unraveling their intricate role in immune response orchestration within both oncological and non-oncological milieus. Deciphering this complex transcriptional regulation holds promise for identifying and demonstrating therapeutic avenues that take advantage of the modulation of DNA and RNA methylation with the goal of alleviating the number of cancer-related morbidity and mortality cases. Conclusion: While more research is required towards fully understanding the effectiveness of epigenetic-based treatments aimed at tumor as well as immune cell populations, there is compelling proof that indicates that they will be successful in slowing the advancement of malignancy as well as lowering cancer-related complications as well fatalities.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"43 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141109845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Endoglucanase and Exoglucanase Enzymes Production by Trichoderma viride Utilizing Olive Mill Wastewater (OMW) in liquid fermentation","authors":"Maram Al Tharf, Muhannad Massadeh, Batol I. Dheeb, Sara N. Abdulla","doi":"10.24126/jobrc.2024.18.1.768","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.768","url":null,"abstract":"Background:The cellulase enzyme is one of the most industrially important enzymes. Its cost represents a significant barrier to other valuable commercial products. Reducing the cost of cellulase production is an important approach. Objectives: For this purpose, this work investigated the production of cellulase enzyme using olive oil production waste (olive mill wastewater (OMW). Materials and methods: The ability of Trichoderma viride to utilize OMW as a substrate for cellulase production was studied. Optimization of cellulase production was investigated to find out the optimum OMW concentration, agitation speed, aeration rate, and cellulose addition. Results:The results showed that 75% v/v OMW submerged shake flask culture was the most suitable culture for T. viride growth and cellulase enzyme production (0.82 U/ml endoglucanase activity and 0.25 U/ml exoglucanase activity). When this culture was supplemented with cellulose, the activity of endoglucanase and exoglucanase was significantly improved (10.24 U/ml and 2.17 U/ml respectively).The agitation speed of 200 rpm enhanced the production to reach 9.1 U/ml of endoglucanase and 6.38 U/ml of exoglucanase. The effect of the aeration rate on enzyme production was studied under batch cultivation. The highest cellulase activity was at 2.0 vvm, where the endoglucanase and exoglucanase activities were 55.96 U/ml and 32.62 U/ml respectively. Conclusions: Therefore, it is claimed that OMW is a suitable medium for cellulase enzyme production after optimization of the process.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"48 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and Health Impact of Heavy Metal Accumulation in (Hair - Nails) of Scavenger Workers at Some Landfill Sites in Baghdad City-Iraq","authors":"Huda A. Khaleel, Adel H. Talib, Maitham A. Sultan","doi":"10.24126/jobrc.2024.18.1.777","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.777","url":null,"abstract":"Background: Heavy metals are considered one of the most dangerous environmental pollutants due to their high toxicity, even at low concentrations, and their accumulation in various body tissues. Landfills contain various wastes rich in heavy metals, such as batteries, paints, and household and hospital waste, making them a major source of air, soil, and groundwater pollution. Objective: To analyze the level of accumulation of heavy metals in hair and nail samples of waste collectors. Material and method: Five main landfill sites in the city of Baghdad were chosen to collect hair and nail samples from waste collectors of both sexes for the winter and summer seasons to analyze the levels of metal concentrations (lead, cadmium, nickel, zinc, and copper). And the use of atomic absorption spectrometry (AAS). Results: Higher concentrations of lead, copper, and nickel were found in nail samples during the summer compared to the winter, indicating an increased risk of exposure to them. While zinc accumulation was much greater in winter samples. Conclusions The results highlight the health risks faced by landfill workers. A need to develop tailored strategies to control exposure to these emissions based on weather conditions and priority metals of concern identified through regular biological monitoring.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":" 832","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141127423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Histopathological Liver Abnormalities Caused by Carbamazepine-Induced Injury in Female Albino Mice","authors":"Nawar R. Jaber, Nahla A. Al-Bakri","doi":"10.24126/jobrc.2024.18.1.778","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.778","url":null,"abstract":"Background: The adverse effects of drugs can damage various organs, especially the liver, leading to a hepatic injury known as hepatotoxicity. Drug-induced liver injury (DILI) is challenging nowadays because of the large number of different drugs used, one of the offending medications that cause DILI is carbamazepine (CBZ), since the liver has an array of functions including detoxification, it will deal with several damages caused by exposure to the drugs. Objective: investigate the effect of (CBZ) 20mg/kg/day on female mice liver after 14 and 30 days of treatment on morphological and histopathological levels. Materials and methods: 20mg/kg/day of CBZ was administered orally for (14) days to (10) female mice, another (10) mice were taking the same concentration for 30 days, and control groups were administered tap water. Results: The findings showed that CBZ can cause liver enlargement, changes in liver appearance, distortion in Glisson’s capsule, cytologic alterations, hepatocyte hypertrophy, ballooning degeneration, pyknosis, karyolysis, karyomegaly, sinusoids dilation, increase in the number and sizes of Kupffer cells, fibrosis, glycogen depletion, and cirrhosis. Conclusion: These findings have shown that carbamazepine (CBZ) can cause hepatotoxicity that can manifest into morphological and histopathological changes.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"20 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Some Immunological Parameters (antioxidant – cytokines) in Cutaneous leishmaniasis in a Sample of Patients in the Al-Ramadi City.","authors":"Othman M. Mohammad, thaer A. SALIH, Mohammad M. Al-Halbosiy","doi":"10.24126/jobrc.2024.18.1.781","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.781","url":null,"abstract":"Background: Cutaneous leishmaniasis is one of the endemic diseases in Iraq. Both types of cutaneous leishmaniasis that cause different ulcers are present, namely Leishmania major and Leishmania tropica, The disease also represents a major public and global health problem all over the world. Objective: Investigation of the relationship between parasitic infection with cutaneous leishmaniasis and some antioxidants Superoxide, Nitric oxide and immune cytokines tumor necrosis factor-alpha, Interferon-gamma. Materials and Methods: The demographic and epidemiological study included 55 cases suspected of being infected with cutaneous leishmaniasis. They visited Ramadi Teaching Hospital, with 25 volunteers. After obtaining their consent, they were used as control samples, where blood serum samples were obtained from both study groups. After that, work was done in laboratories. Department of Biology - College of Education for Pure Sciences - Anbar University. Results: The patients and healthy people were of different genders, and among the 55 samples from the infected, the number of males was 36 (65.5)% and females 19 (34.5)%, with statistically significant differences p<0.05, and the age groups of the patients were from one year to 60 years. With an average age of (31 years), they were distributed into 6 groups. Also, among the 25 samples from the control group, the number of males was 12 (48%) and females were 13 (52%), Their ages also ranged from one year to 60 years, with an average age of (13) years, the enzyme-linked immunoabsorbent assay (ELISA) technique was used to detect the level of immunological indicators TNF-α, IFN-γ, SOD, NO, where the results showed High levels in the serum of cutaneous leishmaniasis patients (120.00 ± 52.11, 123.00 ± 44.21, 3.10 ± 1.21 and 121.93 ± 33.22), respectively compared to healthy subjects (72.86 ± 14.94, 21.91 ± 45.40, 0.88 ± 0.12, and 81.46 ± 4.50 ) respectively With statistically significant differences at the level of p < 0.05, p < 0.001. Conclusions: The inflammatory cytokines TNF-α, IFN-γ play a vital role in increasing or decreasing the severity in the pathogenesis of the disease and eliminating it.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140992087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Neisseria gonorrhea Infection on Gene Expression of p53 and cIAP2 Genes in Cervical Cancer","authors":"Meraim A. Kazaal","doi":"10.24126/jobrc.2024.18.1.767","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.767","url":null,"abstract":"Background: Neisseria gonorrhoeae (N. gonorrhea) is one of the most common bacterial infections causing health problems in the epithelial lining of the cervix. Previous studies did not adequately address its role in cervical cancer (CEC), therefore, the current study aimed to determine the impact of N. gonorrhea infection on gene expression of p53 and cIAP2 in CEC. Materials and Methods: samples were collected from 20 women suffering from cervical cancer with N. gonorrhea infection (CC-NG), and 20 women who had cervical cancer without N. gonorrhea infection (CC) and 40 healthy women as the control group. N. gonorrhea diagnosis was done by Modified Thayer-Martin (MTM) agar and gram stain. Gene expression of p53 and cIAP2 was carried out using real-time PCR. Results: P53  and cIAP2 genes had high molecular expression (folding change) in CC-NG (17.172 and 31.135 respectively) and CC (16.02 and 15.45 respectively) compared to healthy control (1.00) (P>0.05). Moreover, when the expression of both genetic indicators was compared to each other an increase in gene transcription in the cases of CC-NG compared to CC was observed. The antibiotic resistance pattern for the tested Neisseria gonorrhoeae strains was as follows: to ceftriaxone (100%), penicillin (96%), amoxicillin (90%), tetracycline (89%) and ciprofloxacin (77%)  but low to cefixime (4%), spectinomycin (5%) and  azithromycin (9 2%). Conclusion: N. gonorrhea infection can be determine as risk factor for  CEC development.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140995037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Antibiotic Susceptibility of Gram-Negative Bacteria Isolated from Different Meat Samples in Baghdad City","authors":"M. Hadid, A. B. Al-Shaibani, Mohammad Al-Halbosiy","doi":"10.24126/jobrc.2024.18.1.713","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.713","url":null,"abstract":"Background: The occurrence of Gram-negative (G -ve) bacteria in meat samples raises a major concern due to the possibility of drug resistance incidence since G -ve bacteria have built-in resistance mechanisms and can pass on genetic elements that enable other bacterial species to develop into drug-resistant as well. This drug resistance could be transferred to consumers through a food-borne route. This study aimed to evaluate the prevalence of Gram-negative bacteria in meat samples as well as to detect their antibiotic susceptibility patterns. Methods: For this purpose, 100 meat samples (ground meat, raw burgers, frozen chicken, and chicken carcasses) were collected, and obtained isolates were identified using conventional microbiological techniques including cultural and microscopic identification. After that antibiotic susceptibility patterns were detected using Kirby Bauer's disc diffusion method. Results: Results showed that 91 of the samples were harboring Gram-negative bacteria and E.coli was the most common isolate (51.64%) followed by Klebsiella pneumoniae (18.68%) while the least common isolate was each of E. coli O157:H7, Aeromonas hydrophila, Kluyvera spp., Raoultella terrigena, Hafnia alvei, and Serratia marcescens (1.10%). Susceptibility test showed that all isolates were susceptible to Meropenem and Imipenem while Ampicillin was the most resisted antibiotic. Conclusion: We conclude that meat samples harbor numerous pathogenic Gram-negative bacteria which showed antibiotic-resistant ability toward most tested drugs. However, Meropenem and Imipenem were the least resisted drugs which makes them an appropriate choice for the treatment of foodborne infections.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140997703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremoval and Resistance of Some Heavy Metals by Bacterial Isolates from the Sediments of the Diyala River","authors":"Younis A.G. Mijbas, M. A. Fayidh, Raad M. N. Al-Khafaji","doi":"10.24126/jobrc.2024.18.1.763","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.763","url":null,"abstract":"Background:Heavy metals are pollutants that do not decompose, but enter the food chain, and thus form toxic compounds that have a harmful effect on biological functions. There are an unlimited number of organisms in various environments, including bacteria, that are able to degrade and reduce the high levels of many pollutants, most of which have not been as important to researchers as they are interested in pathological microorganisms. Objective: This research sheds light on a number of bacterial isolates that were isolated and identified, one of which showed its high ability to grow in culture environments with high concentrations of heavy metals. Materials and Methods: Twelve sediment samples were collected from the Diyala River within the boundaries of the study area, which included four main sites. Fifteen bacterial isolates capable of resisting heavy metals were recognized at a concentration of (50 mg / L), and then five isolates were selected as the best isolates to grow and resistance of cobalt between (400-750 mg/L), chromium between (1600-2600 mg/L), nickel between (1200-1600 mg/L) and lead between  (1200 - 2200 mg/liter). One of these isolates, (iso 4) showed a high ability to remove heavy metals (chromium, cobalt, nickel, and lead) after the test, with concentrations of (25, 50, and 100 mg/L), and the removal rate for 5 days of chromium was (36, 55, 68, 73 and 77%) respectively, and the removal rate of cobalt was (22, 34, 44, 54 and 59%) and the removal rate of nickel was (23, 40, 56, 68 and 80%) and the removal rate for lead is (58, 68, 74, 80 and 86%). The same isolate also showed high efficiency in removing lead due to the ability to resist high concentrations with a 100% percentage of lead removal at a concentration of 25 mg/L from the first day until the fifth day. The biochemical diagnosis of the selected bacterial isolates was adopted and the diagnosis was made using the VITEK-2 system for isolate No. 4, as the results showed that it belongs to the genus Klebsiella pneumonia. Conclusions: These environmental isolates can be applied in many bioremediations techniques to remove many toxic compounds cheaply and safely.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"37 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140665892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Anatomical Study to The Colchicum szovitsii from Colchicaceae Family in Iraq","authors":"Sura H. Jbarah, A. A. F. Al-Rawi","doi":"10.24126/jobrc.2024.18.1.731","DOIUrl":"https://doi.org/10.24126/jobrc.2024.18.1.731","url":null,"abstract":"Background: Colchicum szovitsii it is a wild perennial herbaceous plant grows in Iraq, it is medical and poisonous herb contain colchicine (alkaloid have cytotoxic effects) especially in corm and seeds. All parts are highly toxic if eaten. Handling of corms may cause skin allergy. Methods: This investigation present morphological and anatomical features of this plant for the first time in Iraq. (3) Results: The corm shape oblong to oblong-ovoid, the shape of the leaf is linear to oblong-lanceolate, undifferentiated perianth with 6 white oblanceolate tepals, the stamens epiphyllous. The anatomical study observed isolateral leaf, uniseriate epidermis and anomocytic stomata, and the mesophyll consisting many layers of irregular spongy paranchyma (chloranchyma), the type of vascular bundle is collateral. Conclusions: In this study we found that C. szovitsii distributed in Iraq have different morphological and anatomical features from the same species in Turkey and Iran like the dimension of corms, leaves, stamens, filament, anthers and capsule.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"28 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140659834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Quality of Public Swimming Pools in Yenagoa Metropolis, Bayelsa State, Nigeria","authors":"Ebidi Appah Embelemi E. Charles, Tariwari C. N Angaye","doi":"10.32861/jbr.92.50.59","DOIUrl":"https://doi.org/10.32861/jbr.92.50.59","url":null,"abstract":"The quality of water in public swimming pools is essential to the wellbeing of tourist and visitors who patronize them. When standard of treatment is compromised it become a threat to the swimmers. This study assessed the levels of heavy metals in 6 randomly selected public swimming pools in Yenagoa Metropolis, Bayelsa State. The sampling and analysis was carried out following standard protocols. Results on were reported for; Temperature (26.16 – 29.82 oC), pH (6.33 – 6.87), residual chlorine (0.44 – 4.14 mg/l), Total dissolved solid (36.18 – 81.44 mg/l), Turbidity (3.49 – 7.17 NTU), and Conductivity (70.22 – 177.94 mg/l). All values recorded were with the WHO permissible limit for recreational water. This study concludes that there is need to formulate the standardization and compliance monitoring as well as operational standards of public swimming pools in order to mitigate potential adverse effects and may arise from standard compromise.","PeriodicalId":15122,"journal":{"name":"Journal of Biotechnology Research Center","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135865241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}