Journal of Camel Practice and Research最新文献_第5页

Protein Profile and Glycosidase Activities of Prostate and Bulbourethral Glands Secretion in Camels (Camelus dromedarius) 骆驼前列腺和球尿道分泌物的蛋白谱及糖苷酶活性

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00026.1

S. El-Bahr, M. Waheed, I. Ghoneim, I. Albokhadaim, A. Meligy, M. E. El Sebaei

{"title":"Protein Profile and Glycosidase Activities of Prostate and Bulbourethral Glands Secretion in Camels (Camelus dromedarius)","authors":"S. El-Bahr, M. Waheed, I. Ghoneim, I. Albokhadaim, A. Meligy, M. E. El Sebaei","doi":"10.5958/2277-8934.2022.00026.1","DOIUrl":"https://doi.org/10.5958/2277-8934.2022.00026.1","url":null,"abstract":"This study was aimed to investigate protein profiles and glycosidase activities of bulbourethral and prostate glands secretion in dromedary camels. Sixteen healthy male camels (5-13 years old) were slaughtered and both glands were collected. The obtained secretions of both glands were used for determination of protein profile by 7-20% gel electrophoresis. Activities of β -N-acetylglucosaminidase, α -N-acetylgalactosaminidase and α -L-fucosidase were also determined. The current findings showed 11 (175.7, 129.3, 126.8, 94.9, 77.8, 74.4, 71.1, 53.3, 21.8, 15.9 and 9 kDa) and 15 (124.6, 89.7, 81.1, 75.5, 72.1, 60, 54.4, 48.9, 43.3, 40, 32.5, 24, 17.8, 13.3 and 9 kDa) protein bands in secretion of bulbourethral and prostate gland, respectively. Three (77.8, 53.3 and 9 kDa) and 6 bands (81.1, 75.5, 48.9, 17.8, 13.3 and 9 kDa) showed higher staining intensities in bulbourethral and prostate secretion, respectively. Both secretions shared a band of 9 kDa with higher intensity in bulbourethral secretion. Activities of glycosidase in bulbourethral and prostate secretions were verified and were higher in bulbourethral than in prostate secretion. α -L-fucosidase showed higher activity in glands secretions compared to other enzymes. Conclusively, bulbourethral and prostate secretions may be involved in coagulation processes of dromedary semen due to their contents of secretory proteins and glycosidases.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duration of MERS – Coronavirus Antibodies in a Small Closed Dromedary Camel Herd in Dubai 中东呼吸综合征冠状病毒抗体在迪拜小型封闭单峰骆驼群中的持续时间

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00039.x

U. Wernery, S. Joseph, M. Rodríguez, N. Paily, S. Thomas, R. Raghavan

引用次数: 0

Light and Electron Microscopy of Buccal Salivary Glands of the Dromedary Camel 单峰骆驼颊唾液腺的光学和电子显微镜观察

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00048.0

Abdelrahman Mohamed Ali Elseory, R. Yasin, Z. H. Ibrahim, A. Ali, K. Alkhodair, T. Althnaian

引用次数: 0

Epididymal Based Changes in Spermatozoa of Dromedary Camels 单峰骆驼精子的附睾变化

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00033.9

D. Rashad, M. Kandiel, A. El-Khawagah, K. Mahmoud, Y. F. Ahmed, M. E. A. El-Roos, G. Sosa

{"title":"Epididymal Based Changes in Spermatozoa of Dromedary Camels","authors":"D. Rashad, M. Kandiel, A. El-Khawagah, K. Mahmoud, Y. F. Ahmed, M. E. A. El-Roos, G. Sosa","doi":"10.5958/2277-8934.2022.00033.9","DOIUrl":"https://doi.org/10.5958/2277-8934.2022.00033.9","url":null,"abstract":"This study was aimed to declare the role of epididymal passage on semen features, DNA integrity and ultra-structures changes in dromedary camel. The testes with attached epididymis of mature dromedary camels (n=50) were collected during the rutting season from December to April. The harvested spermatozoa from the three-main anatomical epididymal segments (Head, body and tail) were assessed for semen characters, DNA integrity as well as, ultra-structural changes. A significant difference in semen features (individual motility, sperm count, membrane and acrosome intactness, viability, normality, maturity, abnormalities and acrosome length and perimeter) among different epididymal segments. Spermatozoa from epididymal tail showed a significantly (p<0.05) lower DNA fragmentation than those collected from head segment while agarose gel electrophoresis revealed non-significant difference in DNA intensity between spermatozoa from the three epididymal regions. Ultra-structures of the epididymal spermatozoa showed changes in acrosome shape, sub-acrosomal space, chromatin condensation and protoplasmic droplet during epididymal passage. The protoplasmic droplets varied in size, density and position with the epididymal segments. In conclusion, epididymal passage (from the head to the tail) is an essential pre-request for dromedary camels’ spermatozoa to attain high fertilising capacity through its great influence on spermatozoa characteristics and the fine cytological structures.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MERS-CoV in Dromedary Camels: Sequence-Based Comparison of Antigenicity and Pathogenicity of Structural and Non-Structural Proteins 单峰骆驼MERS-CoV:结构蛋白和非结构蛋白抗原性和致病性的序列比较

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00014.5

S. El-Bahr, M. Kandeel, I. Albokhadaim, M. Al-Nazawi, H. Ahmed

{"title":"MERS-CoV in Dromedary Camels: Sequence-Based Comparison of Antigenicity and Pathogenicity of Structural and Non-Structural Proteins","authors":"S. El-Bahr, M. Kandeel, I. Albokhadaim, M. Al-Nazawi, H. Ahmed","doi":"10.5958/2277-8934.2022.00014.5","DOIUrl":"https://doi.org/10.5958/2277-8934.2022.00014.5","url":null,"abstract":"The goal of this researcher was to map the camel MERS-CoV structural and non-structural proteins and track changes in antigenicity and pathogenicity from 2013 to 2018. This is critical for developing effective diagnostic and control methods. Therefore, the sequences of MERS-CoV structural proteins such as Spike (S), Membrane (M), Nucleocapsid (N) and Envelope (E) and nonstructural proteins, comprising polyprotein-ab (polyab) and Open reading frame-3 (ORF3) were retrieved. The amino acid sequences of each of these proteins were analysed to estimate their antigenic and pathogenic properties from 2013 to 2018. The antigenicity profiles showed variations in antigenicity minimum, maximum, range, and average from 2013 to 2018. MERS-CoV proteins’ maximum antigenicity score was declining in the examined time frame. In 2013, the maximum score was 0.86, and by 2018, it had dropped to 0.6. This resulted in a greater range of antigenicity with the onset of MERS-CoV in 2013, with a range of 0.56, which eventually decreased to 0.22 in 2018. The net effect on the mean value of antigenicity score revealed that MERS-CoV antigenicity decreased gradually. Between 2013 and 2018, the mean antigenicity score of structural and nonstructural proteins decreased. Non-structural proteins, on the other hand, had the greatest alterations, with a mean value of 0.52 in 2013 and 0.42 in 2018, representing a loss of 19.2 per cent of their antigenicity score. In contrast, the structural protein showed a mean value of 0.55 in 2013 and 0.53 in 2018, i.e. lost only 3.6% of its antigenicity score. Given that ORF3 was nonantigenic, therefore, all changes in antigenicity changes were attributed to polyab protein. Pathogenicity score has decreased from 0.81 in 2013 to 0.78 in 2018. Further studies are required to map these changes on the virus-host cell interaction level.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Neutrophils Extracellular Traps Formation and Reactive Oxygen Species (ROS) Production by Milk Immune Cells from Camels with Subclinical Mastitis 亚临床乳腺炎骆驼乳免疫细胞中性粒细胞胞外陷阱的形成和活性氧(ROS)的产生

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00021.2

J. Hussen, Mohammed Ali Al-Sukruwah, Khulud Bukhari

{"title":"Neutrophils Extracellular Traps Formation and Reactive Oxygen Species (ROS) Production by Milk Immune Cells from Camels with Subclinical Mastitis","authors":"J. Hussen, Mohammed Ali Al-Sukruwah, Khulud Bukhari","doi":"10.5958/2277-8934.2022.00021.2","DOIUrl":"https://doi.org/10.5958/2277-8934.2022.00021.2","url":null,"abstract":"Subclinical mammary gland infections are within the most important infectious diseases in dromedary camels with high impact on milk production and animal health. Using flow cytometry, the present study analysed the capacity of milk phagocytes to form neutrophil extracellular traps (NETs) and to produce reactive oxygen species (ROS) in vitro . Based on the California mastitis test, clinically healthy camels were divided into animals with subclinical mastitis (n = 5) and camels with healthy mammary gland (n = 5). The ex vivo ROS production and the NETs formation activity of milk phagocytes were compared between healthy and affected animals. A basic fraction of phagocytes (10.0 ± 1.7% of total cells) with positive staining with the NETs-sensitive dye SYTOX™ Green was detected in milk samples from healthy camels. The NETs-positive fraction was significantly lower in milk from camels with subclinical mastitis (4.8 ± 1.5 % of total cells) compared to milk samples from healthy camels. Stimulation of milk cells with the gram-negative bacteria E. coli resulted in enhanced ROS production in milk phagocytes from both healthy and affected camels. The two groups, however, did not differ in the ROS level in their unstimulated or stimulated phagocytes. In conclusion, the present study identified basic levels of NETs formation by milk phagocytes separated from healthy camels. The reduced NETs formation by cells from infected camels may play a role in the pathogenesis of subclinical mammary gland infections in camels.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of an in-House Rabbit Anti-Camel Immunoglobulin G (IgG) Conjugated With Horseradish Peroxidase (HRP) For Use in Immunoblots 兔抗骆驼免疫球蛋白G (IgG)与辣根过氧化物酶(HRP)偶联免疫印迹的制备

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00003.0

S. El-Bahr, M.M. El Hassan

引用次数: 0

Gross and Morphometrical Studies on Different Cervical Vertebrae in Dromedary Camels 单峰骆驼不同颈椎的大体和形态计量学研究

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00005.4

G. Singh, B. Meshram, Hemant Joshi, N. Parmar

引用次数: 0

Diagnostic and Predictive Significance of Acute Phase Response and Neopterin Levels in Lame Racing Dromedary Camels (Camelus dromedarius) 跛赛单峰骆驼急性期反应和新蝶呤水平的诊断和预测意义

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00043.1

W. El-Deeb, Mohammed A. Abdelghani

引用次数: 0

The Influence of the Corpus Luteum Location on Hormonal and Vitamin C Composition of Follicular Fluid and Serum in Dromedary Camels (Camelus dromedarius) 黄体位置对单峰骆驼卵泡液和血清激素和维生素C组成的影响

Journal of Camel Practice and Research Pub Date : 2022-01-01 DOI: 10.5958/2277-8934.2022.00049.2

M. Waheed, I. Ghoneim, S. El-Bahr, A. Meligy, I. Albokhadaim, M. El-Sebaei

引用次数: 0