Journal of biological regulators and homeostatic agents最新文献_第9页

Anti-cancer effects of baicalein on cervical carcinoma cells through down-regulation of the ERK/p38/MAPK pathway. 黄芩素通过下调ERK/p38/MAPK通路对宫颈癌细胞的抗癌作用

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-52-A

Y H Luo, L Zhang, M Y Wang, J Fang, J Y Xia, X L Yu

{"title":"Anti-cancer effects of baicalein on cervical carcinoma cells through down-regulation of the ERK/p38/MAPK pathway.","authors":"Y H Luo, L Zhang, M Y Wang, J Fang, J Y Xia, X L Yu","doi":"10.23812/21-52-A","DOIUrl":"https://doi.org/10.23812/21-52-A","url":null,"abstract":"The objective of this study was to investigate the effects of baicalein on apoptosis of HeLa human cervical cancer (CC) cells and to elucidate the underlying mechanism. HeLa cells were treated with 20, 50, 100, or 200 μmol/L baicalein for 24, 36, and 48 hours, and CCK-8 assays were used to detect cell viability, and flow cytometry was performed to assess apoptosis rate. Reverse-transcription quantitative PCR was used to measure ERK1/2, p38, and JNK mRNA levels in HeLa cells, and western blotting was performed to measure ERK1/2, p38, and JNK protein levels. The CCK-8 assay showed that the OD value of HeLa cells gradually decreased with increasing baicalein concentrations (P < 0.01) and treatment time (P < 0.01). These results indicated a negative time- and dose-dependent effect of baicalein on HeLa cells. Baicalein treatment of HeLa cells significantly increased apoptosis rate (P < 0.01). In HeLa cells treated with 50 or 200 μmol/L baicalein for 24 h, expression levels of ERK1/2 and p38 mRNA were significantly reduced, whereas that of JNK mRNA was increased (P < 0.01). The levels of phosphorylated ERK1/2 and p38 were significantly reduced, and the level of JNK protein was increased (P < 0.01). Taken together, baicalein appeared to exert anti-cancer effects on HeLa cells through induction of apoptosis and regulation of the ERK/p38/mitogen-activated protein kinase pathway.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"945-952"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39038455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High cyclic tensile stress disrupts the extracellular matrix in human chondrocyte by F-actin cytoskeletal polymerization and reactive oxygen species production. 高循环拉伸应力通过f -肌动蛋白细胞骨架聚合和活性氧产生破坏人软骨细胞的细胞外基质。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-105-A

Y Li, J Yang, Q Feng, S Q Li, Y Lang, X F Zhang, C Ye

{"title":"High cyclic tensile stress disrupts the extracellular matrix in human chondrocyte by F-actin cytoskeletal polymerization and reactive oxygen species production.","authors":"Y Li, J Yang, Q Feng, S Q Li, Y Lang, X F Zhang, C Ye","doi":"10.23812/21-105-A","DOIUrl":"https://doi.org/10.23812/21-105-A","url":null,"abstract":"This study aims to explore the mechanism of cyclic tensile stress (CTS) on human chondrocytes (CHs) relating to the reactive oxygen species (ROS) generation and extracellular matrix (ECM) stability in vitro. A well-established CTS model with 5%, 10%, or 20% elongation was performed for CHs stretching. After CTS, the cell viability, total ROS level, main ECM components, matrix metalloproteinase (MMP), tissue inhibitor of metalloproteinase (TIMP), F-actin density, and some anti-oxidative enzymes were analyzed. Additionally, the antioxidant N-acetylcysteine (NAC) and cytochalasin D were used to suppress the ROS production and F-actin polymerization when the CHs underwent CTS, respectively. The treatment of 20% elongation-CST significantly decreased the CH viability and the expressions of collagen II, aggrecan, anti-oxidative enzymes and TIMP3/4, however, it increased the ROS accumulation, F-actin polymerization, and the expression of collagen I and MMP3/13. In contrast, the application of NAC and cytochalasin D could partly rescue the CHs from the injury caused by the high CTS. Therefore, high CTS disrupts the ECM by remodeling the F-actin cytoskeleton and promoting ROS production. Cytochalasin D and NAC are effective in rejecting F-actin cytoskeleton polymerization, and ROS accumulation through a potential synergetic process, which alleviates the ECM injury caused by High CTS.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"965-974"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39056303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phageome-based vaccination and human innate immune modulation could be a useful strategy to control human Coronavirus infections. 基于噬菌体的疫苗接种和人类先天免疫调节可能是控制人类冠状病毒感染的有用策略。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/20-592-A

M Abaidullah, S Peng, M Kamran, X Song, A Ali Sher, Y Chen, A Rehman, L Lin, R Jia, Z Yin

{"title":"Phageome-based vaccination and human innate immune modulation could be a useful strategy to control human Coronavirus infections.","authors":"M Abaidullah, S Peng, M Kamran, X Song, A Ali Sher, Y Chen, A Rehman, L Lin, R Jia, Z Yin","doi":"10.23812/20-592-A","DOIUrl":"https://doi.org/10.23812/20-592-A","url":null,"abstract":"Human Coronavirus (CoV) infections, including SARS-COV, MERS-COV, and SARS-CoV-2, usually cause fatal lower and upper respiratory tract infections due to exacerbated expression of pro-inflammatory cytokines and chemokines. We aim to summarize different aspects, such as CoV immune evasion mechanisms and host innate immune response to these infections, and their role in pathogenesis. We have also elaborated the up-to-date findings on different vaccine development strategies and progress against CoVs in both humans and non-human models. Most importantly, we have described the Phageome-human immune interaction, its therapeutic usage as anti-viral, anti-inflammatory agent, and implications for multiple vaccine development systems. The data suggest that endogenous phages might play a vital role in eliminating the infection and regulating the body's immune system. Considering the innate-immune-induced pathogenesis against CoVs and the therapeutic aptitude of phageome, we propose that the prophylactic administration of phages and phage-based vaccines could be a useful strategy to control the emerging CoV infections.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"865-880"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39024312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-20a suppresses RANKL-modulated osteoclastogenesis and prevents bone erosion in mice with rheumatoid arthritis through the TLR4/p38 pathway. MicroRNA-20a通过TLR4/p38途径抑制rankl调节的破骨细胞发生并防止类风湿性关节炎小鼠骨侵蚀。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/20-604-A

X H Kong, S F Shi, H J Hu, J X Wang

{"title":"MicroRNA-20a suppresses RANKL-modulated osteoclastogenesis and prevents bone erosion in mice with rheumatoid arthritis through the TLR4/p38 pathway.","authors":"X H Kong, S F Shi, H J Hu, J X Wang","doi":"10.23812/20-604-A","DOIUrl":"https://doi.org/10.23812/20-604-A","url":null,"abstract":"Abnormal osteoclast formation plays a significant part in rheumatoid arthritis (RA). As potent therapeutic biomarkers, microRNAs (miRNAs) have obtained increasing attention. Recently, treatment regimens regarding miRNAs have been implicated in skeletal diseases. The aim of this study is to assess the expression and function of miR-20a during osteoclast proliferation and differentiation and its correlation with bone erosion in RA mice. The expression of miR-20a was observed to be diminished in the ankle tissues of RA mice relative to that in normal controls evaluated by RT-qPCR. Hematoxylin and eosin staining, Safranin O-fast green staining, and tartrateresistant acid phosphatase staining were used to evaluate the effects of miR-20a on RA symptoms. The proliferation and differentiation of osteoclasts, and bone erosion were repressed by agomiR-20a injection. 3'UTR luciferase reporter assays were conducted to validate the putative binding between miR-20a and receptor activation of nuclear factor-κB ligand (RANKL). The protein expression and phosphorylation level of toll-like receptor4 (TLR4)/p38 pathway-related factors were detected by Western blot. miR-20a inhibited proliferation and differentiation potentials to osteoclasts partly through the TLR4/p38 pathway. The current work provides evidence that miR-20a hinders proliferation and differentiation of osteoclasts by targeting RANKL through the TLR4/p38 pathway.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"921-931"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39156808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Contribution of apolipoprotein estimations to cardiovascular risk assessment in women with endometriosis. 载脂蛋白评估对子宫内膜异位症女性心血管风险评估的贡献。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-207-L

U Rafi, S Ahmad, A Zia, S S Bokhari, M A Iqbal, N Roohi

引用次数: 0

Early childhood caries in Tirana, Albania. 阿尔巴尼亚地拉那的幼儿龋齿。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-26

A Alushi, R J Manenti, M Mema, P Rasicci, E Petro

引用次数: 0

Reduction of radiations exposure in endodontics: comparative analysis of direct (GX S-700, Gendex) and semidirect (VistaScan Mini View, Dürr) digital systems. 减少牙髓学中的辐射暴露:直接(GX S-700, Gendex)和半直接(VistaScan Mini View, d<s:2> rr)数字系统的比较分析

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-11

A Libonati, G Gallusi, E Montemurro, V Di Taranto

引用次数: 0

Impact of self-ligating orthodontic brackets on dental biofilm and periodontal pathogens in adolescents. 自结扎正畸托槽对青少年牙生物膜及牙周病原体的影响。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-13

E Carli, M Pasini, L Lardani, G Giuca, M Miceli

{"title":"Impact of self-ligating orthodontic brackets on dental biofilm and periodontal pathogens in adolescents.","authors":"E Carli, M Pasini, L Lardani, G Giuca, M Miceli","doi":"10.23812/21-3supp1-13","DOIUrl":"https://doi.org/10.23812/21-3supp1-13","url":null,"abstract":"The aim of the present prospective study was to evaluate periodontal health and subgingival microbiological alterations in adolescents treated with fixed self ligating orthodontic brackets in comparison to subject without any orthodontic appliance. A total of 40 adolescents (23 females and 17 males; mean age: 13.2 ± 3.2 years) were included: 30 subjects with self ligating brackets (test group) and 10 patients without orthodontic appliances (control group). Follow-ups were as follows: T1 (1 month), T2 (3 months), T3 (6 months) from the beginning of the orthodontic therapy. Clinical parameters (plaque index, gingival index and clinical attachment level) were measured for every patient and a microbiological analysis was performed. Mann Whitney test was performed to evaluate clinical parameters between test and control group and Friedman test and Fisher test were adopted to evaluate intra group differences at different follow-ups. Student T-test was performed to compare clinical attachment level between the two groups. Significance level was set at p<0.05. No periodontal pathogens and no clinical attachment loss were found in the whole sample. A slightly higher plaque index and gingival inflammation were recorded in the test group in comparison to the controls.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3 Suppl. 1","pages":"107-115"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Guided endodontic microsurgery in apicoectomy: a review. 根管显微手术在根尖切除术中的应用综述。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-7

F Lio, G Mampieri, V Mazzetti, A Leggeri, L Arcuri

{"title":"Guided endodontic microsurgery in apicoectomy: a review.","authors":"F Lio, G Mampieri, V Mazzetti, A Leggeri, L Arcuri","doi":"10.23812/21-3supp1-7","DOIUrl":"https://doi.org/10.23812/21-3supp1-7","url":null,"abstract":"The purpose of this review was twofold. The first aim was to show predictable protocols of guided endodontic microsurgery (EMS) and to describe its clinical outcomes. The second aim was to verify the accuracy of this technique compared to the traditional pathway. An electronic search of publications was established from two electronic databases, Cochrane and PubMed, by two independent researchers. The search strategy used a combination of controlled vocabulary and free-text words. Inclusion and exclusion criteria were defined by the authors before the start of the study. Inclusion criteria were: all studies published in English language; studies in vivo on humans; human cadaver studies; studies analyzing guided surgery in apicoectomy. The initial search yielded 67 citations, of which 10 were included. The studies included in this review analysis have shown two different technique both for digitization of dental arch and for surgical procedures too. Guided endodontic micro-surgery is a more predictable technique with less intra-operative and post-operative complications compared to the \"freehand\" technique and this procedure has shown a greater accuracy.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3 Suppl. 1","pages":"47-55"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A new factor involved during childbirth labor: the importance of having a good dental occlusion. 分娩过程中涉及的一个新因素:良好的牙齿咬合的重要性。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-18

C Maggioni, S Saccomanno, J P Meerssman, R F Mastrapasqua, A Pirino, F Scoppa

{"title":"A new factor involved during childbirth labor: the importance of having a good dental occlusion.","authors":"C Maggioni, S Saccomanno, J P Meerssman, R F Mastrapasqua, A Pirino, F Scoppa","doi":"10.23812/21-3supp1-18","DOIUrl":"https://doi.org/10.23812/21-3supp1-18","url":null,"abstract":"It is widely recognized that the physiology of childbirth labor largely depends on the interaction between three factors: a) the force generated by uterine contractions; b) the structure and characteristics of the birth canal and c) the fetus. Harmony between these three variables determines the initiation of maternal dynamic phenomena and the establishment of an optimal maternal-fetal balance in which both warrant for a correct delivery timing. The present study considered the above known factors and assessed if any other factor, still not recognized, could also play a role, and eventually modify the timing of delivery during the expulsive period. In particular, we focused our attention on the role played by the temporomandibular joint and dental occlusion on maternal body balance and on the stability of muscular reflected forces. The importance of assessing the temporomandibular function and the dental occlusion lies in the fact that any alteration in chewing or in temporomandibular joint (TMJ) mobility and occlusion brings to relevant modifications on the vertebral column and pelvic girdle. Our hypothesis is based on the evidence that those women who have any kind of alteration in their dental occlusion, can have an altered capability of pushing during the expulsive period, as the force applied on the pelvic floor is not expressed. Moreover, recent studies have highlighted a relationship between temporomandibular dysfunctions and sleep apnea syndrome and between sleep apnea syndromes and pregnancy. These relationships are explored in this study.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3 Suppl. 1","pages":"155-166"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39207426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1