Journal of biological regulators and homeostatic agents最新文献_第6页

Long non-coding RNA OIP5-AS1 serves as a competing endogenous RNA to modulate X-linked inhibitor of apoptosis protein expression via adsorbing miR-429 in papillary thyroid cancer. 长链非编码RNA OIP5-AS1作为竞争性内源性RNA，通过吸附miR-429在乳头状甲状腺癌中调节x -连锁凋亡蛋白抑制剂的表达。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/20-666-A

C S Yu, Y B Wang, Q Li, E L Yang, B B Dong

{"title":"Long non-coding RNA OIP5-AS1 serves as a competing endogenous RNA to modulate X-linked inhibitor of apoptosis protein expression via adsorbing miR-429 in papillary thyroid cancer.","authors":"C S Yu, Y B Wang, Q Li, E L Yang, B B Dong","doi":"10.23812/20-666-A","DOIUrl":"https://doi.org/10.23812/20-666-A","url":null,"abstract":"Papillary thyroid cancer (PTC) is currently one of the most common endocrine tumors worldwide. Long non-coding RNA (LncRNA) is a vital regulator in the biological processes of diverse tumors. Hence, this work aimed to clarify the role and mechanism of lncRNA OIP5-AS1 in PTC progression. OIP5-AS1 and miR-429 expression levels in PTC tissues and cells were examined using qRT-PCR. Immunohistochemical staining (IHC) was applied to detect X-linked inhibitors of apoptosis protein (XIAP) expression in PTC tissues. A dual-luciferase reporter gene experiment was employed to validate the relationship for miR-429 and XIAP, miR-429 and OIP5-AS1. The regulatory effects of OIP5-AS1 on PTC cell proliferation, migration, and invasion was detected using the MTT, BrdU, Transwell and Western blot assays. In this work we reported that OIP5-AS1 expression was up-modulated in PTC tissues and cell lines. OIP5-AS1 overexpression enhanced the proliferation and metastasis of PTC cells, but the transfection of miR-429 mimics reversed the functions of OIP5-AS1 on the proliferation, migration, and invasion of PTC cells. Additionally, OIP5-AS1 was identified as a competitive endogenous RNA (ceRNA) that repressed miR-429, thereby increasing the expression level of XIAP. Taken together, the findings confirm that OIP5-AS1 accelerates PTC progression via modulating the miR-429/XIAP axis and imply that OIP5-AS1 is likely to be a therapeutic target for PTC.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39095863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Neurohormonal markers in chronic rhinosinusitis. 慢性鼻窦炎的神经激素标志物。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-35-3-E2

R A Compton, A R Lonergan, I Tsillioni, P Conti, G Ronconi, D Lauritano, E E Rebeiz, T C Theoharides

{"title":"Neurohormonal markers in chronic rhinosinusitis.","authors":"R A Compton, A R Lonergan, I Tsillioni, P Conti, G Ronconi, D Lauritano, E E Rebeiz, T C Theoharides","doi":"10.23812/21-35-3-E2","DOIUrl":"https://doi.org/10.23812/21-35-3-E2","url":null,"abstract":"Chronic rhinosinusitis (CRS), especially with nasal polyps, continues to elude precise pathogenesis and effective treatment. Prior work in our laboratory demonstrated interleukin-33 (IL-33) and Substance P (SP) activation of mast cells, and inhibitory effect of interleukin-37 (IL-37). Our objective is to study the expression of these neurohormonal mediators in mast cell stimulation of nasal polyposis. This was a prospective research study involving collection of nasal lavage fluid and nasal polyp tissue from adult patients with CRS. The study was divided into two arms. First, nasal lavage fluid was collected from normal controls, and patients with allergic rhinitis, CRS, or CRS with nasal polyposis. The second arm was collection of nasal tissue from normal controls undergoing inferior turbinoplasty, or patients with nasal polyposis. Enzyme-linked immunosorbent assay and quantitative polymerase chain reaction techniques were used to determine levels in the lavage fluid and relative gene expression in the tissue of SP, IL-33, and IL-37. In total, 70 lavage and 23 tissue specimens were obtained. The level of SP was highest in patients with polyps; however, gene expression was reduced compared to normal controls. The level of IL-33 was reduced in patients with polyps as compared to patients with allergy and sinusitis, and its gene expression was not significantly different from normal controls. IL-37 was elevated in the lavage fluid of patients with nasal polyps and its gene expression was increased in the polyp tissue. Levels of SP and IL-37 were elevated in the lavage fluid of patients with nasal polyps as compared to normal controls and other sinonasal pathologies, and gene expression of IL-37 was significantly increased in the polyp tissue itself. These findings implicate these neurohormonal molecules in the pathophysiology of nasal polyposis and provide possible novel therapeutic targets.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39159858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Hyaluronic acid and its use in dentistry. 透明质酸及其在牙科中的应用。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-3

D Mucchi

{"title":"Hyaluronic acid and its use in dentistry.","authors":"D Mucchi","doi":"10.23812/21-3supp1-3","DOIUrl":"https://doi.org/10.23812/21-3supp1-3","url":null,"abstract":"The purpose of this paper is to evaluate the role of hyaluronic acid in bio-revitalization (HABR) by testing several extracellular matrix biological parameters in cultured dermal fibroblasts. To this aim, fibroblastic expressed genes after exposition to three HABR medical devices were evaluated. Cells were seeded on a layer of three different medical devices containing 6.2, 10 and 20 mg/ml of HABR for 24 h. Real Time Polymerase Chain Reaction was performed to investigate gene expressions. Genes encoding HABR synthesis and degradation, Metalloproteinases 2 and 3 and Desmoplakin production as well as GDF6, and IGF1 were activated by hyaluronic acid products. The in vitro study showed similar effects on tested genes despite a different concentration of hyaluronic acid contained in the medical devices and the simultaneous presence of other additives. Based on the reported data, gene activations are an aspect of metabolic modulation of signalling pathways rather than the proportional production of a specific connective tissue molecule. Indeed, different HABR concentration and the presence of other additives did not change the overall effect on the studied genes. We believe that the optimization of extracellular matrix micro-environment, obtained by enhanced structural support with HABR, leads to functional and metabolic improvement.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The health of soft tissues around four dental implants loaded immediately supporting a 4-year-old fixed screw-retained prosthesis. 四颗牙种植体周围软组织的健康状况，立即负载支持一个4岁的固定螺钉保留假体。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-8

P Carosi, L Ottria, F Lio, A Laureti, P Papi

{"title":"The health of soft tissues around four dental implants loaded immediately supporting a 4-year-old fixed screw-retained prosthesis.","authors":"P Carosi, L Ottria, F Lio, A Laureti, P Papi","doi":"10.23812/21-3supp1-8","DOIUrl":"https://doi.org/10.23812/21-3supp1-8","url":null,"abstract":"The aim of this study was to assess the soft tissues health around the implant/abutment interfaces of fixed screw-retained prosthesis supported by four dental implants after at least 1-year in function. All the implants were placed between December 1, 2015 and April 30, 2019. Digital implant surgical planning was performed for all the complete-arch rehabilitations and then full-guided surgery was performed. The fixed-interim prostheses were delivered the day of the surgery and replaced by definitive prostheses after the healing period. Patients were followed-up to determinate peri-implant scores, such as Plaque Score (PS) and Bleeding on Probing (BoP). A total of 160 implants were placed in 37 patients, whereas 3 patients received both arches rehabilitated. A total of 40 complete-arch rehabilitations were performed, 26 in the maxilla and 14 in the mandible. Only 5 implants failed resulting in an overall implant survival rate of 96.9%. BoP was detected around 6 implants (3.7%) and 16 implants showed a superficial amount of plaque resulting a Plaque Score of 10%. Within the limitation of this study, it seems that the use of a fixed screw-retained prostheses supported by four dental implants to rehabilitate edentulous jaws could be a valid treatment option in the short and medium term without critical peri-implant issues. However, several perspective studies with longer follow-up are needed to achieve more predictable results.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Conservative treatment of partial rotator cuff tears, comparison between infiltrative treatment with corticosteroids, medium molecular weight hyaluronic acid and high molecular weight hyaluronic acid: a prospective study. 保守治疗部分肩袖撕裂，皮质类固醇、中分子量透明质酸和高分子量透明质酸浸润治疗的比较:一项前瞻性研究。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-132-L

N Setaro, A Gigante

引用次数: 1

Androgen receptor expression in the skin appendages of patients with acne inversa harboring a mutation in the γ-secretase gene NCSTN. γ-分泌酶基因NCSTN突变的痤疮患者皮肤附属物中的雄激素受体表达

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-19-L

T W Shi, N Bai, J A Zhang, F Lu, X D Kong, J B Yu, S S Zhang

引用次数: 0

Hormones and dentistry: a two-way relationship. 激素和牙科:一种双向关系。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-2

D Mucchi

{"title":"Hormones and dentistry: a two-way relationship.","authors":"D Mucchi","doi":"10.23812/21-3supp1-2","DOIUrl":"https://doi.org/10.23812/21-3supp1-2","url":null,"abstract":"Stem cells of dental pulp (SCDPs) are multipotent stem cells with the potential to differentiate into various cell types. For this reason, they have been proposed as an alternative source for mesenchymal stem cells. Somatostatin (ST) is a peptide hormone with an inhibitory effect on several endogenous hormones. The aim of our study is to investigate whether somatostatin can promote or inhibit differentiation of SCDPs in osteoblasts and bone tissue. SCDPs were extracted from third molars of healthy subjects and were treated with ST at the concentration of 100 ng/ml for 24 and 48 h. Gene expression in treated SCDPs was compared with untreated cells (control) to check the effect of somatostatin on stem cell differentiation. After 24 h of treatment many genes investigated were downregulated in treated SCDPs vs untreated SCDPs. Significantly up-regulated gene (Fold change >2) was the Bone Morphogenetic Protein BMP4. On the contrary ST induced the over-expression of bone related genes after 48 h of treatment. TGFB family genes and their receptors were also significantly upregulated after 48 h of treatment. ST demonstrated to promote the self-renewal of SCDPs: in our experiments somatostatin mainly acted on TGFB family genes. Further studies are needed to explore this new way of creating bone tissue.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between TMJ symptoms and orthodontic treatments: a survey on 236 orthodontic patients. 236例正畸患者颞下颌关节症状与正畸治疗的关系

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-22

S Saccomanno, D Laganà, R Mastrapasqua, S Giancaspro, R J Manenti, S Saran

引用次数: 3

ARA290 inhibits high glucose-induced apoptosis of NRK-52E cells. ARA290抑制高糖诱导的NRK-52E细胞凋亡。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-39-L

L J Zhao, X L Sun, J Qiu, B L Xiao, X Y Fan, T Wang, C L Li

引用次数: 1

Be aware of SARS-CoV-2 spike protein: There is more than meets the eye. 注意SARS-CoV-2刺突蛋白:它不仅仅是我们看到的。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/THEO_EDIT_3_21

T C Theoharides, P Conti

{"title":"Be aware of SARS-CoV-2 spike protein: There is more than meets the eye.","authors":"T C Theoharides, P Conti","doi":"10.23812/THEO_EDIT_3_21","DOIUrl":"https://doi.org/10.23812/THEO_EDIT_3_21","url":null,"abstract":"The COVID-19 pandemic necessitated the rapid production of vaccines aimed at the production of neutralizing antibodies against the COVID-19 spike protein required for the corona virus binding to target cells. The best well-known vaccines have utilized either mRNA or an adenovirus vector to direct human cells to produce the spike protein against which the body produces mostly neutralizing antibodies. However, recent reports have raised some skepticism as to the biologic actions of the spike protein and the types of antibodies produced. One paper reported that certain antibodies in the blood of infected patients appear to change the shape of the spike protein so as to make it more likely to bind to cells, while other papers showed that the spike protein by itself (without being part of the corona virus) can damage endothelial cells and disrupt the blood-brain barrier. These findings may be even more relevant to the pathogenesis of long-COVID syndrome that may affect as many as 50% of those infected with SARS-CoV-2. In COVID-19, a response to oxidative stress is required by increasing anti-oxidant enzymes. In this regard, it is known that polyphenols are natural anti-oxidants with multiple health effects. Hence, there are even more reasons to intervene with the use of anti-oxidant compounds, such as luteolin, in addition to available vaccines and anti-inflammatory drugs to prevent the harmful actions of the spike protein.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38992330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19