Journal of biological regulators and homeostatic agents最新文献_第6页

Long non-coding NEAT1 weakens the protective role of sevoflurane on myocardial ischemia/reperfusion injury by mediating the microRNA-140/RhoA axis. 长链非编码NEAT1通过介导microRNA-140/RhoA轴削弱七氟醚对心肌缺血/再灌注损伤的保护作用。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/20-653-A

P F Rui, J H Wang, J Xu

{"title":"Long non-coding NEAT1 weakens the protective role of sevoflurane on myocardial ischemia/reperfusion injury by mediating the microRNA-140/RhoA axis.","authors":"P F Rui, J H Wang, J Xu","doi":"10.23812/20-653-A","DOIUrl":"https://doi.org/10.23812/20-653-A","url":null,"abstract":"The function of long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) has been revealed in injury caused by myocardial ischemia/reperfusion (I/R), however, its association with Sevoflurane (Sev), an anesthetic effective for regulating inflammation and oxidative stress, is not yet clear in I/R injury. The aim of this study was to functionally validate and elucidate the mechanism-of-action for Sev-mediated NEAT1 in myocardial I/R injury. Firstly, reduced NEAT1 was revealed in myocardial I/R injured mice treated with Sev. Moreover, restoration of NEAT1 could repress the alleviating role of Sev in cardiac function, infarct size and myocardial apoptosis in mice, while miR-140 was remarkably enhanced in myocardial tissues from mice treated with Sev. Furthermore, miR-140 was suggested and authenticated as a downstream biomolecule of NEAT1 with the help of a bioinformatics tool. Interestingly, miR-140 inhibitor played the same role as NEAT1 overexpression on the cardiac function, infarct size and apoptosis of mice. Finally, it was manifested that RhoA was a putative target of miR-140, which functioned importantly in the Sev/miR-140-mediated myocardial I/R injury. All in all, NEAT1 knockdown contributed to Sev-mediated myocardial I/R injury alleviation via the miR-140/RhoA axis.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"933-944"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39038457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of miR-425, miR-155 and miR-33 in Streptococcus pneumoniae pneumonia by using bioinformatics analysis and experimental validation. 通过生物信息学分析和实验验证miR-425、miR-155和miR-33在肺炎链球菌肺炎中的潜在作用。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-120-A

C G Chen, B S Luo, C Wang

{"title":"Potential role of miR-425, miR-155 and miR-33 in Streptococcus pneumoniae pneumonia by using bioinformatics analysis and experimental validation.","authors":"C G Chen, B S Luo, C Wang","doi":"10.23812/21-120-A","DOIUrl":"https://doi.org/10.23812/21-120-A","url":null,"abstract":"Streptococcus pneumoniae (S. pneumoniae) pneumonia is the most common cause of community-acquired pneumonia (CAP). Previous studies have suggested the diagnostic potential of microRNAs (miRNAs) in infectious diseases. In the present study, we aimed to evaluate the potential role of miRNAs in S. pneumoniae pneumonia by using bioinformatics analysis and experimental validation. Gene Expression Omnibus (GEO) datasets including GSE97922 and GSE83615 were analyzed for identifying the differentially expressed miRNAs; the miRNA-target genes network was constructed by using miRNet and the targeted genes were subject to Gene Ontology enrichment, Kyoto Encyclopedia of Genes and Genomes and REACTOME pathway analysis; the miRNA and mRNA expression levels were determined by quantitative real-time PCR; protein concentrations were determined by enzyme-linked immunosorbent assay. Our results showed that miR-425, miR-155 and miR-33 were up-regulated in the serum from CAP patients when compared to healthy controls; whereas there was no significant difference in serum miR-222, miR-149, miR-186 and miR-132 expression levels between healthy controls and CAP patients. In vitro functional studies showed that lipopolysaccharides (LPS) induced the up-regulation of miR-425, miR-155 and miR-33 in RAW264.7 cells, and miR-425, miR-155 and miR-33 inhibition attenuated LPS-induced inflammatory responses in RAW264.7 cells. In conclusion, our results showed that miR-425, miR-155 and miR-33 were up-regulated in the serum from CAP patients by using bioinformatics analysis and experimental validation; furthermore, miR-425, miR-155 and miR-33 inhibition attenuated LPS-induced inflammatory responses in RAW264.7 cells. Nevertheless, our studies are still at the preliminary stages, and the detailed roles of miR-425, miR-155 and miR-33 in S. pneumoniae pneumonia still require further investigation.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"953-964"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39056302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Long non-coding RNA OIP5-AS1 serves as a competing endogenous RNA to modulate X-linked inhibitor of apoptosis protein expression via adsorbing miR-429 in papillary thyroid cancer. 长链非编码RNA OIP5-AS1作为竞争性内源性RNA，通过吸附miR-429在乳头状甲状腺癌中调节x -连锁凋亡蛋白抑制剂的表达。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/20-666-A

C S Yu, Y B Wang, Q Li, E L Yang, B B Dong

{"title":"Long non-coding RNA OIP5-AS1 serves as a competing endogenous RNA to modulate X-linked inhibitor of apoptosis protein expression via adsorbing miR-429 in papillary thyroid cancer.","authors":"C S Yu, Y B Wang, Q Li, E L Yang, B B Dong","doi":"10.23812/20-666-A","DOIUrl":"https://doi.org/10.23812/20-666-A","url":null,"abstract":"Papillary thyroid cancer (PTC) is currently one of the most common endocrine tumors worldwide. Long non-coding RNA (LncRNA) is a vital regulator in the biological processes of diverse tumors. Hence, this work aimed to clarify the role and mechanism of lncRNA OIP5-AS1 in PTC progression. OIP5-AS1 and miR-429 expression levels in PTC tissues and cells were examined using qRT-PCR. Immunohistochemical staining (IHC) was applied to detect X-linked inhibitors of apoptosis protein (XIAP) expression in PTC tissues. A dual-luciferase reporter gene experiment was employed to validate the relationship for miR-429 and XIAP, miR-429 and OIP5-AS1. The regulatory effects of OIP5-AS1 on PTC cell proliferation, migration, and invasion was detected using the MTT, BrdU, Transwell and Western blot assays. In this work we reported that OIP5-AS1 expression was up-modulated in PTC tissues and cell lines. OIP5-AS1 overexpression enhanced the proliferation and metastasis of PTC cells, but the transfection of miR-429 mimics reversed the functions of OIP5-AS1 on the proliferation, migration, and invasion of PTC cells. Additionally, OIP5-AS1 was identified as a competitive endogenous RNA (ceRNA) that repressed miR-429, thereby increasing the expression level of XIAP. Taken together, the findings confirm that OIP5-AS1 accelerates PTC progression via modulating the miR-429/XIAP axis and imply that OIP5-AS1 is likely to be a therapeutic target for PTC.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"909-920"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39095863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Neurohormonal markers in chronic rhinosinusitis. 慢性鼻窦炎的神经激素标志物。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-35-3-E2

R A Compton, A R Lonergan, I Tsillioni, P Conti, G Ronconi, D Lauritano, E E Rebeiz, T C Theoharides

{"title":"Neurohormonal markers in chronic rhinosinusitis.","authors":"R A Compton, A R Lonergan, I Tsillioni, P Conti, G Ronconi, D Lauritano, E E Rebeiz, T C Theoharides","doi":"10.23812/21-35-3-E2","DOIUrl":"https://doi.org/10.23812/21-35-3-E2","url":null,"abstract":"Chronic rhinosinusitis (CRS), especially with nasal polyps, continues to elude precise pathogenesis and effective treatment. Prior work in our laboratory demonstrated interleukin-33 (IL-33) and Substance P (SP) activation of mast cells, and inhibitory effect of interleukin-37 (IL-37). Our objective is to study the expression of these neurohormonal mediators in mast cell stimulation of nasal polyposis. This was a prospective research study involving collection of nasal lavage fluid and nasal polyp tissue from adult patients with CRS. The study was divided into two arms. First, nasal lavage fluid was collected from normal controls, and patients with allergic rhinitis, CRS, or CRS with nasal polyposis. The second arm was collection of nasal tissue from normal controls undergoing inferior turbinoplasty, or patients with nasal polyposis. Enzyme-linked immunosorbent assay and quantitative polymerase chain reaction techniques were used to determine levels in the lavage fluid and relative gene expression in the tissue of SP, IL-33, and IL-37. In total, 70 lavage and 23 tissue specimens were obtained. The level of SP was highest in patients with polyps; however, gene expression was reduced compared to normal controls. The level of IL-33 was reduced in patients with polyps as compared to patients with allergy and sinusitis, and its gene expression was not significantly different from normal controls. IL-37 was elevated in the lavage fluid of patients with nasal polyps and its gene expression was increased in the polyp tissue. Levels of SP and IL-37 were elevated in the lavage fluid of patients with nasal polyps as compared to normal controls and other sinonasal pathologies, and gene expression of IL-37 was significantly increased in the polyp tissue itself. These findings implicate these neurohormonal molecules in the pathophysiology of nasal polyposis and provide possible novel therapeutic targets.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"901-908"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39159858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Be aware of SARS-CoV-2 spike protein: There is more than meets the eye. 注意SARS-CoV-2刺突蛋白:它不仅仅是我们看到的。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/THEO_EDIT_3_21

T C Theoharides, P Conti

{"title":"Be aware of SARS-CoV-2 spike protein: There is more than meets the eye.","authors":"T C Theoharides, P Conti","doi":"10.23812/THEO_EDIT_3_21","DOIUrl":"https://doi.org/10.23812/THEO_EDIT_3_21","url":null,"abstract":"The COVID-19 pandemic necessitated the rapid production of vaccines aimed at the production of neutralizing antibodies against the COVID-19 spike protein required for the corona virus binding to target cells. The best well-known vaccines have utilized either mRNA or an adenovirus vector to direct human cells to produce the spike protein against which the body produces mostly neutralizing antibodies. However, recent reports have raised some skepticism as to the biologic actions of the spike protein and the types of antibodies produced. One paper reported that certain antibodies in the blood of infected patients appear to change the shape of the spike protein so as to make it more likely to bind to cells, while other papers showed that the spike protein by itself (without being part of the corona virus) can damage endothelial cells and disrupt the blood-brain barrier. These findings may be even more relevant to the pathogenesis of long-COVID syndrome that may affect as many as 50% of those infected with SARS-CoV-2. In COVID-19, a response to oxidative stress is required by increasing anti-oxidant enzymes. In this regard, it is known that polyphenols are natural anti-oxidants with multiple health effects. Hence, there are even more reasons to intervene with the use of anti-oxidant compounds, such as luteolin, in addition to available vaccines and anti-inflammatory drugs to prevent the harmful actions of the spike protein.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3","pages":"833-838"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38992330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Conservative treatment of partial rotator cuff tears, comparison between infiltrative treatment with corticosteroids, medium molecular weight hyaluronic acid and high molecular weight hyaluronic acid: a prospective study. 保守治疗部分肩袖撕裂，皮质类固醇、中分子量透明质酸和高分子量透明质酸浸润治疗的比较:一项前瞻性研究。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-132-L

N Setaro, A Gigante

引用次数: 1

Androgen receptor expression in the skin appendages of patients with acne inversa harboring a mutation in the γ-secretase gene NCSTN. γ-分泌酶基因NCSTN突变的痤疮患者皮肤附属物中的雄激素受体表达

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-19-L

T W Shi, N Bai, J A Zhang, F Lu, X D Kong, J B Yu, S S Zhang

引用次数: 0

Hormones and dentistry: a two-way relationship. 激素和牙科:一种双向关系。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-2

D Mucchi

{"title":"Hormones and dentistry: a two-way relationship.","authors":"D Mucchi","doi":"10.23812/21-3supp1-2","DOIUrl":"https://doi.org/10.23812/21-3supp1-2","url":null,"abstract":"Stem cells of dental pulp (SCDPs) are multipotent stem cells with the potential to differentiate into various cell types. For this reason, they have been proposed as an alternative source for mesenchymal stem cells. Somatostatin (ST) is a peptide hormone with an inhibitory effect on several endogenous hormones. The aim of our study is to investigate whether somatostatin can promote or inhibit differentiation of SCDPs in osteoblasts and bone tissue. SCDPs were extracted from third molars of healthy subjects and were treated with ST at the concentration of 100 ng/ml for 24 and 48 h. Gene expression in treated SCDPs was compared with untreated cells (control) to check the effect of somatostatin on stem cell differentiation. After 24 h of treatment many genes investigated were downregulated in treated SCDPs vs untreated SCDPs. Significantly up-regulated gene (Fold change >2) was the Bone Morphogenetic Protein BMP4. On the contrary ST induced the over-expression of bone related genes after 48 h of treatment. TGFB family genes and their receptors were also significantly upregulated after 48 h of treatment. ST demonstrated to promote the self-renewal of SCDPs: in our experiments somatostatin mainly acted on TGFB family genes. Further studies are needed to explore this new way of creating bone tissue.","PeriodicalId":15084,"journal":{"name":"Journal of biological regulators and homeostatic agents","volume":"35 3 Suppl. 1","pages":"7-12"},"PeriodicalIF":3.2,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39206508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between TMJ symptoms and orthodontic treatments: a survey on 236 orthodontic patients. 236例正畸患者颞下颌关节症状与正畸治疗的关系

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-3supp1-22

S Saccomanno, D Laganà, R Mastrapasqua, S Giancaspro, R J Manenti, S Saran

引用次数: 3

ARA290 inhibits high glucose-induced apoptosis of NRK-52E cells. ARA290抑制高糖诱导的NRK-52E细胞凋亡。

IF 3.2 4区医学

Journal of biological regulators and homeostatic agents Pub Date : 2021-05-01 DOI: 10.23812/21-39-L

L J Zhao, X L Sun, J Qiu, B L Xiao, X Y Fan, T Wang, C L Li

引用次数: 1