Journal of Biosciences and Medicines最新文献

{"title":"Protective Effect of Liraglutide on Early Renal Fibrosis in Diabetes Mice","authors":"Xiaolan Ke, Tean Ma","doi":"10.4236/jbm.2025.131008","DOIUrl":"https://doi.org/10.4236/jbm.2025.131008","url":null,"abstract":"Background: In recent years, studies have shown that liraglutide may delay the progression of renal fibrosis by inhibiting renal fibrosis signaling pathways and reducing collagen deposition. TGF-β1 and E-Cadherin play crucial roles in renal fibrosis. Objective: To explore the protective effect of liraglutide on early renal fibrosis in diabetes mice. Methods: Twenty-four 8-week-old healthy male C57BL/6J mice were randomly divided into a normal diet feeding group (NG, n = 8) and a high-fat diet feeding group (HG, n = 16) using a simple random sampling method. Four weeks later, the high-fat diet feeding group received a one-time intraperitoneal injection of STZ diluted with 0.1 mol/L sodium citrate buffer (50 mg/Kg). The diabetes model was established after 7 days of continuous injection. The diabetes model mice were randomly divided into 2 groups, each containing 8 mice. One group received liraglutide (400 ug per kilogram per day, subcutaneous injection), named Liraglutide Intervention Group (LDG); the other group received an equal dose of saline subcutaneously, named the Diabetes Model Non-intervention Group (NDG). NG also received an equal dose of saline subcutaneously, named Normal Control Group (NCG). Renal tissue pathological changes were observed by HE and Masson staining; TGF-βl and E-Cadherin protein expressions were detected by immunohistochemistry; E-Cadherin protein expression was detected by Western blotting. Results: The degree of kidney tissue damage and fibrosis in liraglutide intervention group was milder than that in non-intervention group, and the expression of TGF-β1 and E-Cadherin protein tended to be similar to that in normal control group. Conclusion: Liraglutide can significantly reduce early renal fibrosis in diabetes mice, and its mechanism may be related to the reduction of TGF-β1 expression to induce EMT changes in epithelial cells (for example, up regulation of E-Cadherin).","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"13 01","pages":"84-91"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/jbm2025131_82152962.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic Effect of Walnut Peel Water-Soluble Extracts on Lead-Induced PC12 Cytotoxicity","authors":"Tao Jiang, Zongqin Mei, Rongxian Li, Shiyan Gu","doi":"10.4236/jbm.2025.133001","DOIUrl":"https://doi.org/10.4236/jbm.2025.133001","url":null,"abstract":"As a well-recognized environmental contaminant, lead (Pb) represents a profound threat to human health, with the nervous system being especially vulnerable. Pb exerts its toxic effects through a range of specific mechanisms, such as inducing apoptosis, promoting oxidative stress, disrupting ion channel function, impairing neurotransmitter systems, and causing microglial cell damage within neurons. Previous studies have demonstrated that Walnut Peel Extracts possess substantial antioxidant properties. However, varying extraction methods and solvents yield different bioactive compounds. In our study, we employed varying concentrations of Walnut Peel Water-Soluble Extracts (WPWE) to counteract Pb toxicity. Specifically, we utilized the MTT assay, Hoechst staining, and fluorescence probes to assess cell viability, apoptosis rates, and ROS levels. The results indicate that WPWE can effectively mitigate the toxic effects of Pb on PC-12 cells.","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"13 03","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/jbm2025133_12153079.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT1 Regulates Autophagy and Activation of Microglia through Smad3/TRIB3 Signaling Axis to Improve Spinal Cord Injury","authors":"Li Qian, Fucui Tang, Dekun Tang, Yuanmou Li, Wenyi Wang, Mei Qin, Na Yin, Ping Zhan, Xiaodan Liu, Na Chen","doi":"10.4236/jbm.2025.133003","DOIUrl":"https://doi.org/10.4236/jbm.2025.133003","url":null,"abstract":"Background and Purpose: Spinal cord injury (SCI) manifests as a central nervous system disorder causing sensory and motor impairments. Preventing an overabundance of microglial activation is crucial in managing SCI. SIRT1 as a NAD-dependent deacetylase has the effect of ameliorating SCI, but its mechanism of action in microglia activation is still poorly understood. The objective of this research was to explore the role and process of SIRT1 in activating microglia during SCI. Methods: Laminectomy was conducted on the thoracic T10 region of rats, followed by striking the revealed spinal cord using an IH-0400 spinal cord impactor to create a model for SCI animals (n = 30). To create a model of BV2 inflammation, BV2 cells underwent a 24 h treatment with 1 μg/mL LPS. The expression of related proteins was detected by Western blot. Immunofluorescence staining, HE staining and Nissl staining were used to evaluate BV2 cell activation and spinal cord tissue injury in rats. Results: The research indicated minimal expression of SIRT1 in SCI cases, with its heightened expression ameliorating pathological damage and boosting neuron survival rates in SCI rats. Conversely, treatment with LPS markedly reduced autophagy in BV2 cells. Elevated levels of SIRT1 enhanced the production of autophagy-associated proteins LC3-II/I and Beclin1, reduced p62 expression, and curtailed the expression of the activation indicator IBA-1 in BV2 cells. Mechanistically, overexpression of SIRT1 inhibits the expression of TRIB3 by inhibiting Smad3 nuclear metastasis, thereby activating autophagy, inhibiting microglial overactivation and alleviating the development of SCI. Conclusion: Enhancing SIRT1 expression could reduce SCI progression by curbing the overactivation of microglia.","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"13 03","pages":"27-41"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/jbm2025133_32153042.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of Aqueous Extract of Marigold on Arsenic-Induced Oxidative Damage in Pancreatic Islet <i>β</i>-Cells","authors":"Zongqin Mei, Jiao Dai, Guofen Liu, HE Zuo-shun, Shiyan Gu","doi":"10.4236/jbm.2024.125003","DOIUrl":"https://doi.org/10.4236/jbm.2024.125003","url":null,"abstract":"Roles of Marigold extracts (ME) on arsenic trioxide (ATO)-induced oxidative damage to pancreatic β-cells need to be further elucidated. In this study, NIT-1 cells were treated with different concentrations of and/or ATO, following by the cell viability was detected by CCK8 assay. Then, intracellular reactive oxygen species (ROS) levels, lipid peroxide (MDA) contents and superoxide dismutase (SOD) activity were measured with a fluorescence probe method and colorimetric assay, respectively. The apoptosis rate and morphology was detected and observed with hoechst 33,258 staining assay. The mRNA levels and protein expressions of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were measured by real-time fluorescence quantitative polymerase chain reaction and protein immunoblotting assay, respectively. Our results indicated that Co-treatment with ME and ATO exacerbated the cell viability decreasing reduced by ATO, while the addition of ME after ATO treatment effectively promote the recovery of ATO reduced survival rates. The ATO group increased apoptosis (P P β-cells by modulating the activation of the Nrf2 signaling pathway.","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"12 05","pages":"19-34"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scirp.org/pdf/jbm2024125_32152511.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical Modeling and Computer Simulation of a Meander Line Antenna for Alzheimer's Disease Treatment, a Feasibility Study.","authors":"Felipe P Perez,&nbsp;Maryam Rahmani,&nbsp;Jorge Morisaki,&nbsp;Farhan Amran,&nbsp;Syazwani Bakri,&nbsp;Akmal Halim,&nbsp;Alston Dsouza,&nbsp;Nurafifi Mohd Yusuff,&nbsp;Amran Farhan,&nbsp;James Maulucci,&nbsp;Maher Rizkalla","doi":"10.4236/jbm.2023.112013","DOIUrl":"https://doi.org/10.4236/jbm.2023.112013","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a brain disorder that eventually causes memory loss and the ability to perform simple cognitive functions; research efforts within pharmaceuticals and other medical treatments have minimal impact on the disease. Our preliminary biological studies showed that Repeated Electromagnetic Field Stimulation (REFMS) applying an EM frequency of 64 MHz and a specific absorption rate (SAR) of 0.4 - 0.9 W/kg decrease the level of amyloid-<i>β</i> peptides (A<i>β</i>), which is the most likely etiology of AD. This study emphasizes uniform E/H field and SAR distribution with adequate penetration depth penetration through multiple human head layers driven with low input power for safety treatments. In this work, we performed numerical modeling and computer simulations of a portable Meander Line antenna (MLA) to achieve the required EMF parameters to treat AD. The MLA device features a low cost, small size, wide bandwidth, and the ability to integrate into a portable system. This study utilized a High-Frequency Simulation System (HFSS) in the design of the MLA with the desired characteristics suited for AD treatment in humans. The team designed a 24-turn antenna with a 60 cm length and 25 cm width and achieved the required resonant frequency of 64 MHz. Here we used two numerical human head phantoms to test the antenna, the MIDA and spherical head phantom with six and seven tissue layers, respectively. The antenna was fed from a 50-Watt input source to obtain the SAR of 0.6 W/kg requirement in the center of the simulated brain tissue layer. We found that the E/H field and SAR distribution produced was not homogeneous; there were areas of high SAR values close to the antenna transmitter, also areas of low SAR value far away from the antenna. This paper details the antenna parameters, the scattering parameters response, the efficiency response, and the E and H field distribution; we presented the computer simulation results and discussed future work for a practical model.</p>","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"11 2","pages":"177-185"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9187534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal <i>TMPRSS6</i> Gene Polymorphism rs855791SNP in Women with Preeclampsia","authors":"Yasir I. B. Ahmed, Hind S. Yagoub, Mohamed A. Hassan","doi":"10.4236/jbm.2023.111008","DOIUrl":"https://doi.org/10.4236/jbm.2023.111008","url":null,"abstract":"","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74783222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances on the Treatment of Allergic Rhinitis by Traditional Chinese Medicine and Western Medicine","authors":"Wenjing Lu","doi":"10.4236/jbm.2023.114010","DOIUrl":"https://doi.org/10.4236/jbm.2023.114010","url":null,"abstract":"","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78218732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Bioremediation Potential and Antimicrobial Activity of <i>Pseudomonas</i> and Bacillus Bacteria Isolated from Landfills in Brazzaville, Congo","authors":"Adriana Préfina Nzoumba Pandi, S. Mokémiabeka, J. Bidounga, Tarcisse Baloki Ngoulou, R. Moyen","doi":"10.4236/jbm.2023.118011","DOIUrl":"https://doi.org/10.4236/jbm.2023.118011","url":null,"abstract":"","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81734717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating College Students’ Diet and Physical Activity Behaviors Regarding Non-Communicable Diseases Prevention in China","authors":"L. Hung, Yueyuan Chen, Yuqi Huang, Li-Ching Hung, Me-Teng Hung","doi":"10.4236/jbm.2023.112025","DOIUrl":"https://doi.org/10.4236/jbm.2023.112025","url":null,"abstract":"","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84343234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Human Papillomavirus Infection in Cervical Exfoliated Cells","authors":"Yuan Xu, Qi Wen, Zheng Zhao, Ke Zhang, Shuying Li","doi":"10.4236/jbm.2023.118017","DOIUrl":"https://doi.org/10.4236/jbm.2023.118017","url":null,"abstract":"","PeriodicalId":15067,"journal":{"name":"Journal of Biosciences and Medicines","volume":"375 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85468177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}