Journal of capillary electrophoresis最新文献_第6页

A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins 一种在低丰度血清蛋白中发现潜在疾病标志物的简单且高度可重复性的方法

Journal of capillary electrophoresis Pub Date : 2009-03-01 DOI: 10.2198/JELECTROPH.53.13

Y. Kawashima, Tomoyuki Fukuno, M. Satoh, Hiroki Takahashi, T. Matsui, T. Maeda, Y. Kodera, Y. Kodera

引用次数: 9

Glycoproteomic analysis of abnormal N-glycosylation on the kappa chain of cryocrystalglobulin in a patient of multiple myeloma 一例多发性骨髓瘤患者结晶球蛋白kappa链异常n -糖基化的糖蛋白组学分析

Journal of capillary electrophoresis Pub Date : 2009-03-01 DOI: 10.2198/JELECTROPH.53.1

T. Toda, Megumi Nakamura, Masaki Yamada, T. Nishine, Tomohiro Torii, K. Ikenaka, Ryouta Hashimoto, M. Mori

{"title":"Glycoproteomic analysis of abnormal N-glycosylation on the kappa chain of cryocrystalglobulin in a patient of multiple myeloma","authors":"T. Toda, Megumi Nakamura, Masaki Yamada, T. Nishine, Tomohiro Torii, K. Ikenaka, Ryouta Hashimoto, M. Mori","doi":"10.2198/JELECTROPH.53.1","DOIUrl":"https://doi.org/10.2198/JELECTROPH.53.1","url":null,"abstract":"SUMMARY Crystalglobulinemia (cryocrystalglobulinemia) is a rare complication of multiple myeloma. Crystallization of immunoglobulin in blood circulation causes systemic vasculopathy especially in skin and kidney. We found a rare case of crystalglobulinemia in which the light chain was N-glycosylated. The abnormal N-glycosylation was primarily detected as the molecular mass shift on SDS-PAGE by PNGase F treatment. The cryocrystalglobulin was shown to be composed of 55-kDa heavy and 32-kDa light chains on SDS-PAGE. However, the apparent molecular masses of them shifted to 51 kDa and 28 kDa, respectively by PNGase-F treatment. The cryocrystalglobulin was identified as an IgG κ type by peptide mass fingerprinting. The N-glycans on the κ light chain were assigned to non-fucosylated biantennary oligosaccharides and their bisected forms by MALDI-TOF MS/MS analysis of glycopeptides. Sialylation of the abnormal N-glycans was suggested by linear-mode MS and confirmed by HPLC analysis. The N-glycosylation consensus Asn (Asn-Xxx-Ser/Thr) was found in the glycopeptide at the N-glycosylation site determined as “EIVMTQSPANLSVLPGER” by MALDI-TOF MS/MS, in which the consensus Asn (N) was converted to Asp (D) in the enzymatically deglycosylated peptide.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"65 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2009-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72979704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

遺伝子診断（検査）の最新の話題基因诊断(检查)的最新话题

Journal of capillary electrophoresis Pub Date : 2008-12-15 DOI: 10.2198/SBK.52.177

豊輝森部

引用次数: 0

Divergent roles of tumor suppressor gene Klf6 in ES cell differentiation 肿瘤抑制基因Klf6在ES细胞分化中的不同作用

Journal of capillary electrophoresis Pub Date : 2008-12-01 DOI: 10.2198/JELECTROPH.52.65

N. Matsumoto, N. Matsumoto, Xiao Zhao, F. Itoh, S. Friedman

{"title":"Divergent roles of tumor suppressor gene Klf6 in ES cell differentiation","authors":"N. Matsumoto, N. Matsumoto, Xiao Zhao, F. Itoh, S. Friedman","doi":"10.2198/JELECTROPH.52.65","DOIUrl":"https://doi.org/10.2198/JELECTROPH.52.65","url":null,"abstract":"Klf6 is a zinc finger transcription factor that has been identified as a tumor suppressor gene inactivated in several human cancers. We previously reported that Klf6-/- mice are embryonic lethal because of impaired hematopoietic differentiation. In that study, we also found Klf6-/- mice have undefinable livers. Combined with this observation and its developmental expression pattern, Klf6 might have a role in in differentiation of non-hematopoietictissues. The aim of this study was to more clearly define the roles of Klf6 in tissue specification using tissue-specific ES cell differentiation systems. We have evaluated three different culture conditions to induce ES cell differentiation, comparing the responses between Klf6+/+ and Klf6-/- ES cells. Neuronal differentiation of Klf6-/- ES cells was impaired based on marker gene expression and morphology. Interestingly, Klf6 -/- ES cells began to express Hnf3β mRNA, which identified these cells as endodermal precursors, suggesting that Klf6 ordinarily suppresses endoderm differentiation in this culture condition. Additionally, when Klf6-/- ES cells were grown in StemPro34 serum free medium, they showed enhanced cardiac differentiation to a greater extent than Klf6+/+ ES cells, suggesting that Klf6 may have an inhibitory activity in preventing cardiomyogenesis. These findings indicate divergent and tissue- specific roles of Klf6 in ES cell differentiation.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"20 1","pages":"65-69"},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82160961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

マイクロチップミセル動電クロマトグラフィーにおける新規オンライン試料濃縮法：トランジェント－トラッピング法の開発微芯片显像动电色谱的新型在线样品浓缩法:开发瞬移-补漏法

Journal of capillary electrophoresis Pub Date : 2008-09-15 DOI: 10.2198/SBK.52.155

文彦北川, 健治末吉, 浩二大塚

引用次数: 0

High performance RNA separation by in-capillary denaturing gel electrophoresis with carboxylic acid as RNA denaturant 以羧酸为RNA变性剂的毛细管内变性凝胶电泳高效分离RNA

Journal of capillary electrophoresis Pub Date : 2008-09-15 DOI: 10.2198/SBK.52.133

Keiko Sumitomo, Y. Yamaguchi

{"title":"High performance RNA separation by in-capillary denaturing gel electrophoresis with carboxylic acid as RNA denaturant","authors":"Keiko Sumitomo, Y. Yamaguchi","doi":"10.2198/SBK.52.133","DOIUrl":"https://doi.org/10.2198/SBK.52.133","url":null,"abstract":"For RNA size separation in a small sample volume (<10 nL), a strong denaturant to cleave the intramolecular hydrogen bonds that maintain the high-order structures of RNA and optimization for a small sample volume are required. We suggested, “in-capillary denaturing gel electrophoresis” as the RNA separation based on capillary gel electrophoresis, that realizes the denaturation and separation simultaneously in a capillary tube. We found that carboxylic acids were strong denaturants for in-capillary denaturing gel electrophoresis, and the performance of RNA separation was dramatically improved with a running buffer containing acetic acid. Based on the decrease of DNA melting temperature, we estimated that the denaturing ability of 2.0 M acetic acid was stronger than that of either 2.5 M formaldehyde or 7.0 M urea. The baseline separation of RNA with a size of 100−10,000 nt was achieved in only 25 min by in-capillary denaturing gel electrophoresis containing 2.0 M acetic acid. The resolution and number of plates of RNA separation were higher and larger than those obtained in a conventional capillary gel electrophoresis with sample preparation with 7.0 M urea.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"60 1","pages":"133-138"},"PeriodicalIF":0.0,"publicationDate":"2008-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84554737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reduced immunoreactivity of urinary albumin in patients with cardiovascular diseases: Analysis of immunochemically nonreactive albumin 心血管疾病患者尿白蛋白免疫反应性降低:免疫化学非反应性白蛋白的分析

Journal of capillary electrophoresis Pub Date : 2008-09-01 DOI: 10.2198/JELECTROPH.52.57

Akinobu Nakayama, Jyunichi Nishimaki, T. Kawara, T. Kasama, Toshiaki Baba, H. Yoshida, M. Isobe, K. Shiba, Kenji Sato

{"title":"Reduced immunoreactivity of urinary albumin in patients with cardiovascular diseases: Analysis of immunochemically nonreactive albumin","authors":"Akinobu Nakayama, Jyunichi Nishimaki, T. Kawara, T. Kasama, Toshiaki Baba, H. Yoshida, M. Isobe, K. Shiba, Kenji Sato","doi":"10.2198/JELECTROPH.52.57","DOIUrl":"https://doi.org/10.2198/JELECTROPH.52.57","url":null,"abstract":"We analyzed 55 spot urine samples from patients with cardiovascular diseases. Urinary albumin concentrations were measured with size exclusion high performance liquid chromatography (HPLC), turbidimetric immunoassay (TIA) using anti human serum albumin polyclonal antibody, and enzyme-linked immunosorbent assay (ELISA) using anti human serum albumin monoclonal antibody. Fractionated urine samples from the HPLC were also analyzed with the immunoprecipitation reactions using as same monoclonal antibody as ELISA. As a result, the urinary albumin concentration analyzed by the HPLC was systematically higher than that of immunoassays, however, ‘albumin peak’ from the HPLC contained other urinary proteins. Result of immunoprecipitation reaction showed the presence of monomer albumin that could not react with the monoclonal antibody. These results suggest that not only contamination of other proteins, the albumin fraction from the HPLC included albumin with reduced its reactivity to the specific monoclonal antibody.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"52 1","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80808377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tissue specificity of tropomyosin isoform in the mussel, Mytilus galloprovincialis 贻贝(Mytilus galloprovincialis)原肌球蛋白亚型的组织特异性

Journal of capillary electrophoresis Pub Date : 2008-09-01 DOI: 10.2198/JELECTROPH.52.47

A. Itoh, M. Fujinoki

引用次数: 4

Gene expression of PROSC (proline synthetase co-transcribed) in related to cytosine arabinoside sensitivity in human leukemia PROSC(脯氨酸合成酶共转录)基因表达与人白血病中阿糖胞嘧啶敏感性相关

Journal of capillary electrophoresis Pub Date : 2008-09-01 DOI: 10.2198/JELECTROPH.52.53

S. Fujimaki, Mayu Takeda, Kuniaki Saito, T. Funato

引用次数: 0

Automatic transfer of polyacrylamide gels to protein chip plates for protein electroblotting 聚丙烯酰胺凝胶自动转移到蛋白质芯片板，用于蛋白质电印迹

Journal of capillary electrophoresis Pub Date : 2008-06-01 DOI: 10.2198/JELECTROPH.52.43

Akiko Okayama, Yoko Ino, Keita Mishima, Takeshi Okada, Y. Iwafune, Noriaki Arakawa, H. Kawasaki, H. Hirano

{"title":"Automatic transfer of polyacrylamide gels to protein chip plates for protein electroblotting","authors":"Akiko Okayama, Yoko Ino, Keita Mishima, Takeshi Okada, Y. Iwafune, Noriaki Arakawa, H. Kawasaki, H. Hirano","doi":"10.2198/JELECTROPH.52.43","DOIUrl":"https://doi.org/10.2198/JELECTROPH.52.43","url":null,"abstract":"Protein chips are useful tools for profiling proteins and analyzing protein-protein interactions and post-translational modifications. In previous work, we developed a diamond-like carbon-coated stainless steel plate (DLC plate) as a novel protein chip plate. Gel-resolved proteins can be covalently immobilized on the surface of the DLC plate by electroblotting to produce a high-density protein chip. The proteins can then be identified by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS) using the plates. The interactions of the immobilized proteins with other proteins, and their post-translational modifications, can also be analyzed. However, to improve the efficiency and reproducibility of analyses using DLC plates, it is important to automate these analytical processes. Therefore, we developed a system for automatically transferring gels from the gel electrophoresis glass plates to the DLC plates for electroblotting. This is an essential first step toward complete automation of the production of high-density protein chips for immobilizing gel-resolved proteins.","PeriodicalId":15059,"journal":{"name":"Journal of capillary electrophoresis","volume":"24 1","pages":"43-46"},"PeriodicalIF":0.0,"publicationDate":"2008-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77966241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0