{"title":"Isolation and Antibiogram Profiles of Staphylococcus aureus Isolates from Cow milk and Dog samples","authors":"A. Zedan, N. Alatfeehy, S. Marouf","doi":"10.21608/javs.2022.164610.1181","DOIUrl":"https://doi.org/10.21608/javs.2022.164610.1181","url":null,"abstract":"Staphylococcus aureus ( S. aureus ) is a commensal bacterium associated with serious infections in men and animals. Recently, multidrug-resistant (MDR) strains of S. aureus especially the so-called methicillin-resistant S. aureus (MRSA), represent a serious challenge that hinders the control of infections in man and animals. This study aimed to monitor milk samples from mastitic cows and vaginal and nasal swabs from dogs for the incidence of S. aureus . In addition, the isolates' antibiogram profiles were assessed to determine the extent of MDR and MRSA existence among the recovered isolates. Out of 260 samples, 29 (11%) S. aureus isolates were recovered with the highest incidence in milk samples (15/90, 17%), followed by vaginal swabs (8/90, 9%) and nasal swabs (6/80, 7%). Identification of the isolates was confirmed by PCR amplification of 16S rRNA gene sequence. Twenty S. aureus isolates were tested against seven antibacterial agents. Surprisingly, all the twenty isolates were MRSA and three bitch vaginal isolates were MDR. The findings of this study call for more research and cooperation between authors interested in assessing the MRSA and MRD bacterial incidence in both medical and veterinary fields. The cooperation will augment the challenge of disseminating MRSA and MDR staphylococci from animals to humans and vice versa.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46682105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis","authors":"S. Abodalal, M. Ismail, Eman El Rawy","doi":"10.21608/javs.2022.163930.1179","DOIUrl":"https://doi.org/10.21608/javs.2022.163930.1179","url":null,"abstract":"Riemerella anatipestifer ( R. anatipestifer ) infection and duck virus hepatitis (DVH) are enormous dangers for the duck industry and its investment. So, in the current study, a combined inactivated vaccine against both of them was prepared to combat their adverse effect. One hundred and thirty-three ducks of one-day-old of age were used and grouped randomly into four groups. Group (1) was vaccinated with R. anatipestifer vaccine, group (2) was vaccinated with DHV vaccine, group (3) was vaccinated with the prepared combined vaccine of both and finally, group (4) was kept as a negative control. Vaccination was at one day old of age. The vaccinated groups with Riemerella vaccine had 72.7% protection against challenges with the virulent strain with the highest antibody titers in 6 th week as measured by the indirect Hemagglutination test. The control group had 90.9% mortality when challenged against R. anatipestifer , with no detectable antibody titers. DVH-vaccinated groups exhibited their highest serum-neutralizing antibody titers by the 5 th and 6 th weeks post-vaccination. The Control group had no detectable antibody titers against DVH. Statistically, it was clear that there were no significant differences between the results of different groups vaccinated with combined or single vaccines of the same agent. Briefly, combined vaccines of R. Anatipestifer and duck viral hepatitis have harmonized effects with the priority to decrease the stress on birds and workers. Besides its efficiency, the economic side as providing one-shot vaccines instead of each one separately.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47641407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detergent Maceration: A Convenient Skeleton Preparation Technique for Teaching and Demonstration of Veterinary Anatomy","authors":"Md. Ashraf Zaman Faruk, S. Das","doi":"10.21608/javs.2022.158955.1175","DOIUrl":"https://doi.org/10.21608/javs.2022.158955.1175","url":null,"abstract":"The current study investigates to develop a convenient technique for preparation of a dog skeleton without any unpleasant smell, cracking, crumbling or discoloration of bones by using a combination of commercially available detergent and Hydrogen peroxide (H 2 O 2 ). Specimens were skinned and immersed in a pre-heated 10-liter detergent solution at 50°C to 60°C for 2-3 hours then left for 12 hours. Maceration was deemed completed when any remaining soft tissue was easily removed under running tap water or by soft brush. Then the bones were kept for bleaching with 3% H 2 O 2 for 24-48 hours and allowed to dry properly in the sun. It is shown that the detergent maceration was remarkably faster compared to the traditional burial, insect, hot or cool-water methods, which requires up to several days. In this entire process, skeletons were prepared and installed in correct conformation in only about 92 hours (less than four days). In contrast, the whole maceration was completed in 15 hours (boiling and cooling). On the other hand, the longest time was taken for bleaching and drying purposes, which were 2 days, respectively. The method investigated here for the preparation of skeletal materials in an essentially odorless way, which has shown to be more convenient (faster, easier, safer, and cheaper) compare to previous models in the fields of veterinary anatomy. We believe this project's result will help improve the preparation and preservation technique of skeletons for gross anatomical studies in veterinary institutions.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47096538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdel-Azeem Abdel-Latif, Fatma H. M. A., Jehan M. M. Ouf, Mohamed H. H. Roby, N. Abdel-Atty
{"title":"Polycyclic Aromatic Hydrocarbons in Grilled Meats from Restaurants","authors":"Abdel-Azeem Abdel-Latif, Fatma H. M. A., Jehan M. M. Ouf, Mohamed H. H. Roby, N. Abdel-Atty","doi":"10.21608/javs.2022.158847.1174","DOIUrl":"https://doi.org/10.21608/javs.2022.158847.1174","url":null,"abstract":"Polycyclic aromatic hydrocarbons are a group of lipophilic compounds that can be generated during the preparation of food items at elevated temperatures. They are regarded as potentially genotoxic and carcinogenic to human beings, related to increased incidence of breast and colorectal cancers, oxidative DNA injury, and bad effects on children neuro-differentiation. Thus, they are considered a public health concerns. A total of thirty samples of grilled beef steak, beef kofta and chicken (ten each) were collected from different restaurants. The samples were extracted by magnesium sulfate and sodium acetate in acetonitrile then purified in magnesium sulfate, primary, secondary amine and silica gel, and finally measured by gas chromatography-mass spectrometry (GC-MS). Benzo[a]pyrene was recorded with the highest average level (3.63µg/kg) in grilled kofta samples, but it was not detected in chicken samples. On the other hand, PAH4, PAH8 and ƩPAHs content were more abundant in grilled beef steak (5.32, 9.97 and 56.91µg/kg). Meanwhile, they recorded the least concentrations of grilled chicken from different restaurants. Furthermore, benzo[a]pyrene exceeded the permissible limits of the European Commission and Egyptian National Food Safety Authority in grilled kofta samples; further studies are needed to investigate the limits of exposure to these harmful compounds from meats and other food items. ــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68513553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Adding Bee Pollen on The Sexual Efficiency of Quail Males","authors":"M. Yonis, A. Hassan","doi":"10.21608/javs.2022.164168.1180","DOIUrl":"https://doi.org/10.21608/javs.2022.164168.1180","url":null,"abstract":"This study aims to determine the role of bee pollen in improving the reproductive efficiency of quail males raised at high density. The total number was 288 birds at 7 weeks old, randomly distributed to 6 experimental groups. The 1 st group was placed at a rate of 21 birds / m 2 , 2 nd group was placed at a rate of 21 birds / m 2 and addition of bee pollen 20 g/kg feed, the 3 rd group was at a rate of 21 birds / m 2 addition of bee pollen 30 g/kg feed, the 4 th group was high density 75 birds / m 2 , 5 th group was high density75 birds/ m 2 and supplemented with bee pollen 20 g/kg feed, and the 6 th group was high density 75 birds/m 2 and supplemented with bee pollen 30 g/kg feed. The results showed that adding 20g and 30g of bee pollen to the diet of 21 birds caused a significant increase in testosterone hormone. In contrast, the high density of birds caused a significant decrease in testosterone, sperm count and live sperm accompanied by an increase in the dead and deformed sperm. Furthermore, decrease in body weight. Adding 20g and 30g of bee pollen to the high density of birds resulted in a significant increase in testosterone, sperm count, and live sperm accompanied by a reduction in dead and deformed sperm and an increase in body weight. The study concluded that the high density of the birds' numbers had a negative impact on the bird's reproductive efficiency and body weight while adding bee pollen to the diet improved quail male body weight and reproductive efficiency.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48276142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sero-prevalence of Border Disease Virus Antibodies in Recently Introduced Dorper Sheep Flocks at Debre Birhan Agricultural Research Center, Ethiopia","authors":"Chekol Getahun, Tadiwos Dessie, Eridachew Tesema, Enyiew Alamirew","doi":"10.21608/javs.2022.155122.1171","DOIUrl":"https://doi.org/10.21608/javs.2022.155122.1171","url":null,"abstract":"A study was conducted to investigate the serological prevalence of Border Disease Virus (BDV) antibodies in newly introduced Dorper sheep flocks at Debre Birhan Agricultural Research Center (DBARC), Amhara Region, Ethiopia. Blood samples were collected from the jugular vein of 186 sheep in the research center and the serum was decanted and sent to the National Animal Health Diagnostic and Investigation Center, Sebeta, Ethiopia for laboratory examination. All serum samples were examined using blocking ELISA test with a sensitivity of 94.3% and specificity of 93.7% to detect antibodies for BDV. Of these, 55 (29.57%) tested sero-positive and the rest 131 (70.43%) tested sero-negative for BDV antibodies. The sero-prevalence of sheep born in the research center was found at 23.97% (29/121) whereas the sero-prevalence of those sheep imported directly from abroad was found at 40% (26/65). In this research, a higher prevalence of BDV antibodies were recorded in the imported sheep than in the sheep which are born in the research center and the difference in prevalence between the two groups was found statistically significant (P<0.05). Therefore, it is of utmost importance to study the status of the disease from indigenous breeds of sheep and utilize more sensitive tests such as RT-PCR.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44233133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Struvite Bladder Stone in a Cat with Hyperthyroidism","authors":"E. Koral, C. Er, A. Barut","doi":"10.21608/javs.2022.155293.1172","DOIUrl":"https://doi.org/10.21608/javs.2022.155293.1172","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49378720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Some Biochemical changes induced by Toxic Effects of Sulfur in Mice","authors":"F. Hassan, B. Al-baggou","doi":"10.21608/javs.2022.155557.1173","DOIUrl":"https://doi.org/10.21608/javs.2022.155557.1173","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47621815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zainab. J. Mohammed, Rizgar Nabi, Y. Mousa, M. Mahmood
{"title":"Identification of Histopathological Changes Induced by Amitraz in Rats","authors":"Zainab. J. Mohammed, Rizgar Nabi, Y. Mousa, M. Mahmood","doi":"10.21608/javs.2022.154375.1169","DOIUrl":"https://doi.org/10.21608/javs.2022.154375.1169","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47724226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Aiyedun, A. Darajat, Daodu B.O., Oludairo O.O., I. Olorunshola, Nwoha R.I.O., I. Oluboyede
{"title":"Assessment of the Knowledge, Attitude and Practices of Dog Owners on Rabies in Ilorin, Kwara State, Nigeria","authors":"J. Aiyedun, A. Darajat, Daodu B.O., Oludairo O.O., I. Olorunshola, Nwoha R.I.O., I. Oluboyede","doi":"10.21608/javs.2022.154332.1168","DOIUrl":"https://doi.org/10.21608/javs.2022.154332.1168","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43570943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}