Journal of Applied Bioanalysis最新文献_第2页

Automated Liquid Handling for Microplate Assays: a Simplified User Interface for the Hamilton Microlab STAR 微孔板测定的自动液体处理:汉密尔顿微实验室STAR的简化用户界面

Journal of Applied Bioanalysis Pub Date : 2021-01-15 DOI: 10.17145/JAB.21.003

Neville S Ng, Reece D. Gately, L. Ooi

{"title":"Automated Liquid Handling for Microplate Assays: a Simplified User Interface for the Hamilton Microlab STAR","authors":"Neville S Ng, Reece D. Gately, L. Ooi","doi":"10.17145/JAB.21.003","DOIUrl":"https://doi.org/10.17145/JAB.21.003","url":null,"abstract":"Automated liquid handling stations such as the Hamilton Microlab range can be implemented to greatly enhance throughput of cell-based and cell-free assays. To facilitate utilisation past the comprehensive programming interface of the Hamilton Method Editor this paper presents a user interface (UI) that runs within Hamilton Venus and allows for a user to control and store programs for plate-toplate transfers and serial dilutions in 96 well plate format. The interface allows for rapid control of aspiration and dispensing height, trituration, control of tip rack selection, and includes a tip washing program that can reduce the number of disposable tips utilised. The simple program interface allows the Hamilton Microlab to be used as a readily deployable microtitre plate handler, particularly for applications such as aliquoting cells for seeding, diluting a number of plates in medium, or transferring drug dilution arrays to multiple plates. This paper also discusses various optimisations to increase accuracy and rate of low volume liquid transfer. While complex liquid handling tasks such as high-throughput drug screening requires established core facilities, standalone liquid handlers with simplified interfaces can be utilised for smaller-scale research applications and educational purposes. Citation: Ng N, Gately R, Ooi L. Clinical A simplified interface for handling microtitre plate assays with the Hamilton Microlab STAR J Appl Bioanal 7(1), 11-18 (2021). Received: April 01, 2020. Revised: June 23, 2020. Accepted: June 27, 2020. Open Access & Copyright: ©2021 Ng N et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and source are","PeriodicalId":15014,"journal":{"name":"Journal of Applied Bioanalysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79199944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Regulatory Perspective on Bioassay Implementation Strategies for Potency Evaluation of Biopharmaceuticals 生物药品效价评价生物测定实施策略的监管视角

Journal of Applied Bioanalysis Pub Date : 2021-01-15 DOI: 10.17145/JAB.21.002

A. Pepe

引用次数: 0

Welcome to the seventh Volume of Journal of Applied Bioanalysis 欢迎来到《应用生物分析杂志》第七卷

Journal of Applied Bioanalysis Pub Date : 2021-01-15 DOI: 10.17145/jab.21.001

R. Meesters

引用次数: 0

Perspectives on Multi-Omics and Omics Applications in Biomedical Research: an Interview with Dr. Biswapriya B. Misra 多组学和组学在生物医学研究中的应用前景:采访Biswapriya B. Misra博士

Journal of Applied Bioanalysis Pub Date : 2020-08-15 DOI: 10.17145/jab.20.011

B. Misra

引用次数: 0

LC-MS/MS assay for the simultaneous quantitation of thromboxane B2 and prostaglandin E2 to evaluate cyclooxygenase inhibition in human whole blood LC-MS/MS法同时测定人全血中血栓素B2和前列腺素E2对环加氧酶的抑制作用

Journal of Applied Bioanalysis Pub Date : 2020-08-15 DOI: 10.17145/jab.20.014

Yifan Shi, H. Murrey, Kay Ahn, Naidong Weng, Shefali R Patel

引用次数: 1

Multi-omics for Biomedical Applications 生物医学应用的多组学

Journal of Applied Bioanalysis Pub Date : 2020-08-15 DOI: 10.17145/jab.20..012

M. S. Khan, J. Azmir

引用次数: 1

LC-MS/MS Method for the Quantitative Determination of Tanespimycin and its Active Metabolite in Human Plasma: Method Validation and Overcoming an Insidious APCI Source Phenomenon LC-MS/MS法定量测定人血浆中丹那霉素及其活性代谢物:方法验证及克服APCI源隐匿现象

Journal of Applied Bioanalysis Pub Date : 2020-08-15 DOI: 10.17145/jab.20.015

Morse Faria, O. Ismaiel, James Waltrip, Thomas Mariannino, Moucun Yuan, W. Mylott, V. Roongta, Jim X. Shen, Pathanjali Kadiya

引用次数: 0

Clinical Applications of Circulating Tumor DNA, Circulating Tumor Cells, and Exosomes as Liquid Biopsy-Based Tumor Biomarkers 循环肿瘤DNA、循环肿瘤细胞和外泌体作为液体活检肿瘤生物标志物的临床应用

Journal of Applied Bioanalysis Pub Date : 2020-08-15 DOI: 10.17145/jab.20.013

Xiaoshuang Yan, Chao-Chi Yeh, Linglong Zou

{"title":"Clinical Applications of Circulating Tumor DNA, Circulating Tumor Cells, and Exosomes as Liquid Biopsy-Based Tumor Biomarkers","authors":"Xiaoshuang Yan, Chao-Chi Yeh, Linglong Zou","doi":"10.17145/jab.20.013","DOIUrl":"https://doi.org/10.17145/jab.20.013","url":null,"abstract":"Along with improved knowledge of cancer biology and biotechnical progress, the diagnostic approaches have evolved from tissue biopsies to liquid biopsies. As they provide a minimally invasive tumor detection, liquid biopsies allow early diagnosis and serial assessments of tumor progression. Discovery and use of circulating tumor markers circulating tumor DNA (ctDNA), circulating tumor cells (CTC), and exosomes have largely expanded the possibility of early diagnosis of cancer, patient stratification, as well as developing a personalized treatment. Based on these circulomes, liquid biopsies can be developed, but each type of liquid biopsies has its own merits and limitations. While ctDNA-based methods represent the most advanced techniques, sensitivity improvement is expected given the rarity of ctDNA in circulation. As intact cancer cells, CTC provide information on cancer cells. However, current CTC capturing procedures are still lack of efficiency. Exosomes are abundant, but they are highly heterogeneous and there is a lack of specific markers for identification. Future efforts are needed to improve operational parameters and clinical performance of each method. Prior to a broad use in clinical settings, it is crucial to standardize the procedure for the specific liquid biopsy method and validate the test with adequate specificity and sensitivity for clinical applications.","PeriodicalId":15014,"journal":{"name":"Journal of Applied Bioanalysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86783902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Quantification of Levofloxacin in Saliva 液相色谱-串联质谱法定量唾液中左氧氟沙星的交叉验证

Journal of Applied Bioanalysis Pub Date : 2020-06-15 DOI: 10.17145/jab.20.008

S. Ghimire, Erwin M. Jongedijk, Simone H J van den Elsen, Mireille A. Wessels, D. Touw, J. Alffenaar

引用次数: 5

Bioanalysis as a powerful tool in Dentistry: the case of short-term and long-term release of Monomers from dental Composites 生物分析作为牙科的有力工具:牙科复合材料中单体的短期和长期释放情况

Journal of Applied Bioanalysis Pub Date : 2020-06-15 DOI: 10.17145/jab.20.010

E. Diamantopoulou, V. Samanidou

{"title":"Bioanalysis as a powerful tool in Dentistry: the case of short-term and long-term release of Monomers from dental Composites","authors":"E. Diamantopoulou, V. Samanidou","doi":"10.17145/jab.20.010","DOIUrl":"https://doi.org/10.17145/jab.20.010","url":null,"abstract":"Novel materials used in preventive and restorative dentistry contain monomers with endocrine or cytotoxic properties, which can cause minimum or even severe damage to human body, when found in specific concentrations. The degradation of resin composite restorations after aging and/or storage in different solutions is associated with leaching monomers, like bisphenol Aglycidyl methacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), Urethane dimethacrylate (UDMA), Bisphenol A (BPA), which are potentially leading to toxicity and mutagenicity effects or cause allergic reactions. These monomers may cause health issues to patients, therefore their determination both in-vitro and in biological fluids e.g. saliva, blood serum/plasma and urine is significant. Moreover, analytical methods are necessary to investigate the rate of elution, as well as the conditions that mainly affect the mechanism of short-term and long-term release of monomers from dental composites. In this review article we present some of the techniques and methods used to determine the short-term and long-term release of these monomers from modern dental materials and prove that analytical chemistry and especially bioanalysis can be a powerful tool in dentistry","PeriodicalId":15014,"journal":{"name":"Journal of Applied Bioanalysis","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83464496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3