Journal of Advanced Science最新文献_第10页

Erratum: Experimental simulation of the closed deivertor system for nuclear fusion, (Vol.23, 3&4, pp.22-25) 勘误:核聚变封闭发射器系统的实验模拟，(Vol.23, 3&4, pp.22-25)

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.23.E1

Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, K. Kawamura

引用次数: 0

Isolation of repetitive sequence elements from the genome of a mayfly Rhithrogena japonica (Ephemeroptera: Heptageniidae) 日本蜉蝣(蜉蝣目:七叶蝇科)基因组重复序列元件的分离

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.24.1

Pathirana Erandi, Takemon Yasuhiro, Takayanagi Atsushi, M. Eman, Tanida Kazumi, Shimizu Nobuyoshi, Kato Mikio

{"title":"Isolation of repetitive sequence elements from the genome of a mayfly Rhithrogena japonica (Ephemeroptera: Heptageniidae)","authors":"Pathirana Erandi, Takemon Yasuhiro, Takayanagi Atsushi, M. Eman, Tanida Kazumi, Shimizu Nobuyoshi, Kato Mikio","doi":"10.2978/JSAS.24.1","DOIUrl":"https://doi.org/10.2978/JSAS.24.1","url":null,"abstract":"Isolation of short, tandem repeat sequences from mayfly Rhithrogena japonica Uéno 1928 has been carried out by probing the multicopy DNA regions using labeled total genomic DNA. Several microsatellites comprised of pyrimidine/purine-biased (Pyr/Pur) repeating units (CT, TCTG, and TCTCTG) and two minisatellites were identified. No locus containing (CA) n repeat has been identified so far by screening with total genomic DNA and (CA) 25 probes. The Pur/Pyr microsatellites may be more abundant than (CA) n microsatellite in the R. japonica genome. Both minisatellite sequences, termed Rja66 family (107bp repeating unit) and Taq I-family (repeating unit about 160bp), have self-complementary regions within the repeating units, thereby conforming to stem-loop structures in single-stranded DNA. As for microsatellite loci, a preliminary analysis on two microsatellites Rja18-1 (CT repeat, FJ827088) and Rja130F (TCTCTG repeat, FJ826512) was done for a local population of R. japonica , showing that they were highly polymorphic. Characterization of genetic structures of local R. japonica populations using Rja18-1 locus, demonstrated significant differences between geographically distant populations indicating an impaired genetic flow between such populations.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72959272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental simulation of the closed deivertor system for nuclear fusion 核聚变封闭发射体系统的实验模拟

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.23.22

Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, T. Iijima, K. Kawamura

引用次数: 2

Fabrication of collagen gel fiber films utilizing PELID method 利用PELID法制备胶原凝胶纤维薄膜

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.23.14

H. Akiyama, S. Umezu, H. Hashimoto

{"title":"Fabrication of collagen gel fiber films utilizing PELID method","authors":"H. Akiyama, S. Umezu, H. Hashimoto","doi":"10.2978/JSAS.23.14","DOIUrl":"https://doi.org/10.2978/JSAS.23.14","url":null,"abstract":"In recent years, a higher level of medical technology has been required. One such technology is regenerative medicine. In regenerative medicine, much research has been done on stem cells. Likewise, much research has been done on scaffold fabrication techniques. Ink-jet techniques have attracted attention as a method of fabrication of a three-dimensional scaffold. However, scaffolds fabricated by ink-jet techniques require further research. In this study, we have fabricated a highly biocompatible scaffold utilizing an ink-jet technique. The fabrication utilized an electrostatic ink-jet technology called the PELID method. Micro-fiber scaffolds must be suitable for the growth of cells, and film thickness must also be able to be controlled by a few microns. The scaffolds that we have fabricated were designed to satisfy these two requirements, using collagen. We fabricated the scaffolds in spinning mode, which is one of the ink-jet modes. When an applied voltage was high, the diameter of fibers narrowed and film thickness became uniform. In addition, we layered a film of this collagen (scaffold) on MDCK cells, and the cells grew well. We demonstrated that it is possible to fabricate scaffolds with a high biocompatibility by using this method. In the future, we hope to fabricate simple layered body tissue by applying this method.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84746995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TFA-MOD法YBCO超伝導テープ線材を用いた2 kA級電流リードユニットの開発采用tft - mod法YBCO超导胶带线材开发2ka级电流导线单元

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.23.19

Haruki Motohashi, Yuichi Ishii, Yukiko Sakata, Yutaka Yamada, K. Tachikawa

引用次数: 0

Experimental study of stabilized supply flow rate on a small bore journal sliding bearing 小口径滑动轴承稳定供给流量的实验研究

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.24.24

Y. Naruse, M. Ochia

引用次数: 3

ヒューマンセキュリティからみた3.11東日本大震災・原子力災害後の日本のエネルギー政策に関する研究从人类安全看3.11东日本大地震和核灾后日本能源政策研究

Journal of Advanced Science Pub Date : 2012-01-01 DOI: 10.2978/JSAS.24.29

Tamehiro Ko, Uchida Hirohisa, Nakamura Kouhei