JOR Spine最新文献

{"title":"Single-Cell Analysis Integrated With Machine Learning Elucidates the Mechanisms of Nucleus Pulposus Cells Apoptosis in Intervertebral Disc Degeneration and Therapeutic Interventions","authors":"Chao Song,&nbsp;Xiaofei Wu,&nbsp;Chaoqi Chen,&nbsp;Baoxin Shen,&nbsp;Yongliang Mei,&nbsp;Qian Yan,&nbsp;Feng Jiang,&nbsp;Feng Chen,&nbsp;Fei Liu","doi":"10.1002/jsp2.70036","DOIUrl":"10.1002/jsp2.70036","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The molecular of intervertebral disc degeneration (IVDD) is still unclear. When it comes to treating decoction, traditional Chinese medicine is effective. In particular, the Duhuo (Radix Angelicae Biseratae) may be particularly helpful.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>To identify nucleus pulposus cells (NPCs) subpopulations and immune cells and clarify the mechanism of IVDD therapy, offering recommendations for diagnosis and treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>IVDD targets from the Genecards and microarray data from biological databases. To find the key genes and biological pathways underlying IVDD, multiple machine learning techniques were used. IVDD is associated with subpopulations of NPCs as revealed by single-cell analysis, and immunological infiltration was identified by Immune Cell AI. To validate the molecular pathways by which Duhuo activity affects IVDD, network pharmacology and molecular docking were employed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The process of IVDD is linked to key genes like TP53, JUN, PTEN, IL1B, ERBB2, MAPK8, CASP9, PTK2, etc. The main molecular mechanisms involved in this process are immune responses, inflammatory factors expression, cellular responses to mechanical stimuli, and NPC apoptosis. Immune Cell AI discovered a correlation between CD4 naïve, B cell, monocyte, NK, and macrophage infiltration with the development of IVDD. The NPC subtypes associated with IVDD, namely fibroNPCs, adhesion NPCs, regulatory NPCs, homeostatic NPCs, and hypertrophic chondrocyte-like NPCs (HT-CL NPCs), were the subject of single-cell mapping. We also found that Osthole, Columbianadin, and Bergapten, the principal blood entry components of Dohuo, may have a role by modulating CASP9, MAPK8, PTGS1, and PARP1, the targets of apoptosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The NPC subpopulations that exist in IVDD are HT-CL NPCs, fibroNPCs, adhesion NPCs, regulatory NPCs, and homeostatic NPCs. Furthermore, a variety of immune cell infiltrates, particularly monocyte and macrophage, have a significant impact on the advancement of IVDD. Osthole, Columbianadin, and Bergapten, the principal components of Duhuo, absorb IVDD via controlling the death of NPCs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral Lumbo-Pelvic Fixation for Denis Type I Unilateral Sacral Fracture: A Finite Element Analysis","authors":"Jie Yang,&nbsp;Kai O. Böker,&nbsp;Xishan Li,&nbsp;Xiang Zhou,&nbsp;Wolfgang Lehmann","doi":"10.1002/jsp2.70022","DOIUrl":"10.1002/jsp2.70022","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Unilateral sacral fractures with posterior ring instability represent a prevalent type of posterior pelvic ring fracture. While lumbo-pelvic fixation is recognized as a highly stable method, the sufficiency of unilateral lumbo-pelvic fixation (ULF) for such fractures remains under debate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>This study aims to assess the biomechanical stability of ULF compared to traditional bilateral lumbo-pelvic fixation (BLF) and triangular osteosynthesis (TO), incorporating clinical observations, and previous biomechanical data.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We developed a three-dimensional spine-pelvis model to simulate a unilateral sacral fracture with posterior ring instability. The model was used to compare the stability of ULF with BLF and TO, utilizing both newly generated data and ULF models reported in existing literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our findings indicate that BLF and TO provide greater stability than ULF, with BLF emerging as the most stable model. While ULF may be insufficient for immediate postoperative weight-bearing, TO also demonstrated potential risks of instability during rotational and lateral bending movements toward the fracture side.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Despite its application in clinical settings, ULF may not adequately support early postoperative mobility. This study underscores the need for cautious application of ULF and suggests that enhancements such as additional fixation points may be necessary. The results also highlight the importance of tailored postoperative rehabilitation strategies for patients undergoing TO, especially in managing movements that could destabilize the fracture site.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11745017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Dynamic Growth Rod Inducing Spinal Growth Modulation for the Correction of Spinal Deformities","authors":"Yangyang Xu,&nbsp;Da Lu,&nbsp;Le Zhang,&nbsp;Shijia Zhang,&nbsp;Yong Wu,&nbsp;Heng Li,&nbsp;Baoqing Pei,&nbsp;Xueqing Wu","doi":"10.1002/jsp2.70031","DOIUrl":"10.1002/jsp2.70031","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Growth rods are the gold standard for treating early-onset scoliosis (EOS). However, current treatments with growth rods do not optimize spinal growth in EOS patients, and frequent distraction surgeries significantly increase complications, imposing considerable economic and psychological burdens on patients. An improved growth rod is urgently required to address the need for dynamic growth and external regulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study designed a novel growth rod (NGR) with unidirectional sliding and external regulation capabilities. By establishing a three-dimensional model of the EOS spine, we simulated the implantation of traditional growth rods (TGR) and NGR. We applied a compressive load of 400 N to test axial stiffness and a moment of 1 NM to assess bending stiffness under six different conditions. Additionally, we evaluated the range of motion (ROM) of the spinal joints, and the distribution of Von Mises stress in vertebrae, intervertebral discs, and the growth rods, and calculated the axial force, moment, fatigue life, and strain energy of the device.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NGR exhibits higher axial compression and torsional stiffness than TGR and the Intact group. Additionally, Von Mises stress values for NGR are higher than those for TGR across all operating conditions, albeit with slightly lower total strain energy than TGR. Although Von Mises stress in NGR concentrates near the screw fixation, the fatigue life remains adequate for basic living requirements.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Overall, NGR demonstrates superior stiffness and stress distribution. NGR's distraction-based implant features a unidirectional sliding component with a spring-driven mechanism for dynamic correction and a novel non-invasive extension mechanism to reduce infections. Compared to leading EOS implants, NGR offers improved stability, showing promise for enhancing EOS surgical interventions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Key Differential Metabolites in Intervertebral Disc Degeneration Based on Untargeted Metabolomics","authors":"Daqian Zhou,&nbsp;Xingrui Zhang,&nbsp;Jiale Lv,&nbsp;Yongliang Mei,&nbsp;Yingjin Luo,&nbsp;Fengjiang Li,&nbsp;Zongchao Liu","doi":"10.1002/jsp2.70032","DOIUrl":"10.1002/jsp2.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Intervertebral disc degeneration disease (IVDD) is a prevalent orthopedic condition that causes chronic lower back pain, imposing a substantial economic burden on patients and society. Despite its high incidence, the pathophysiological mechanisms of IVDD remain incompletely understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to identify metabolomic alterations in IVDD patients and explore the key metabolic pathways and metabolites involved in its pathogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Serum samples from 20 IVDD patients and 20 healthy controls were analyzed using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC–MS). The identified metabolites were mapped to metabolic pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant alterations were observed in metabolites such as 2-methyl-1,3-cyclohexadiene, stearoyl sphingomyelin, methylcysteine, L-methionine, and cis, cis-muconic acid. These metabolites were involved in pathways including glycine, serine, and threonine metabolism, cyanoamino acid metabolism, and the citrate cycle (TCA cycle).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The identified metabolic alterations provide insights into the pathogenesis of IVDD and suggest potential therapeutic targets for future investigation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between ADAMTS-4 and ADAMTS-5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study","authors":"Cansu Tuncer,&nbsp;Emin Mehmet Eminoğlu,&nbsp;Ömer Emre Yağlı,&nbsp;Gülçin Baş,&nbsp;Ahmet Sancar Topal,&nbsp;Ece Onur,&nbsp;Habib Özdemir,&nbsp;Şemsi Güliz Yılmaz,&nbsp;Gülgün Yılmaz Ovalı,&nbsp;Cüneyt Temiz","doi":"10.1002/jsp2.70037","DOIUrl":"10.1002/jsp2.70037","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Study Design</h3>\u0000 \u0000 <p>Prospective biochemical study of comparison of A Disintegrin and Metalloproteinase with Thrombospondin motifs-4 (ADAMTS-4) and A Disintegrin and Metalloproteinase with Thrombospondin motifs 5 (ADAMTS5) levels in preoperative and postoperative venous blood, as well as in disc tissue obtained during surgery, in patients undergoing surgery for intervertebral disc disease, with enzyme levels in venous blood from a control group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To compare the levels of ADAMTS-4 and ADAMTS-5 between patients with degenerative intervertebral discs and a healthy control group, aiming to identify biomarkers associated with intervertebral disc degeneration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Literature</h3>\u0000 \u0000 <p>Although numerous studies have investigated the relationship between ADAMTS-4 and ADAMTS-5 enzymes and degeneration in experimental rat models and human tissues, no study has correlated their serum levels with intervertebral disc degeneration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method and Materials</h3>\u0000 \u0000 <p>Venous blood samples were obtained preoperatively and postoperatively from 41 patients (age: 42 ± 9.7 years, range 20–63) diagnosed with intervertebral disc disease. The affected disc levels were L4-L5 in 22 patients and L5-S1 in 19 patients. These patients were selected based on surgical indications due to radicular pain that persisted after an adequate course of conservative management, without any non-neurological deficit. Disc tissue samples were also obtained during surgery. Additionally, venous blood samples were collected from a control group with no diagnosed diseases, and lumbar MRIs of the control group showed no significant signs of degeneration. ADAMTS-4 and ADAMTS-5 levels were measured using the ELISA method on samples obtained after centrifugation of the collected blood and tissue specimens.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The level of ADAMTS-4 in patient serum was found to be lower compared to the control group, while the level of ADAMTS-5 was higher in the patient serum and lower in the control group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Elevated levels of ADAMTS-5 in the blood may be associated with intervertebral disc degeneration.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Causal Relationship Between the Plasma Levels of MMP1 (Matrix Metalloproteinase-1), MMP3, MMP7, MMP10, and MMP12 and Intervertebral Disc Degeneration: Mendelian Randomization","authors":"Lin Sun,&nbsp;Kai-Qing Yan,&nbsp;Qi Zhang,&nbsp;Ji Ma,&nbsp;Bo Shi,&nbsp;Xi-Yuan Yang,&nbsp;Li-Jun Li","doi":"10.1002/jsp2.70034","DOIUrl":"10.1002/jsp2.70034","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Several matrix metalloproteinases (MMPs) have been reported to be associated with intervertebral disc degeneration (IDD) in several previous studies. However, the causal relationship between MMPs and IDD remains unclear. In this study, Mendelian randomization (MR) was used to analyze the causal relationship between the plasma levels of multiple MMPs and the risk of IDD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The GWAS data of the plasma levels of MMP1, MMP3, MMP7, MMP10, and MMP12 were derived from the genome-wide variation associations of 21 758 European individuals. The genetic associations of the variants with IDD were investigated in the largest genome-wide association study from GWAS pipeline using Phesant derived variables from UKBiobank (1045 cases; 461 965 controls). We used a two-sample MR method to evaluate the causal relationship between these five MMPs and IDD. The causal effects were examined by inverse variance weighted (IVW) test. And sensitivity analysis was performed using Q test of IVW and MR-Egger, MR-Egger-intercept and MR-PRESSO.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We found a significant correlation between increased the plasma level of MMP3 and an increased risk of IDD (IVW: OR 1.000564, 95% CI 1.0000304–1.00110; <i>p</i> = 0.0383). The heterogeneity test (MR-Egger Q test: <i>p</i> = 0.346 and IVW Q test: <i>p</i> = 0.460) indicated that there was no heterogeneity in this instrumental variable on the surface. Also, no directional horizontal pleiotropy was observed in the MR analysis (MR-Egger, <i>p</i> = 0.708 and MR-PRESSO, <i>p</i> = 0.609). There was no significant correlation between the plasma levels of MMP1, MMP7, MMP10, and MMP12 and an increased risk of IDD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our MR analysis found that there is a potential causal relationship between increased the plasma level of MMP3 and the risk of IDD in the European population. There is no potential causal relationship between the plasma levels of MMP1, MMP7, MMP10, and MMP12 and an increased risk of IDD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Clinical Insights: New Radiographic Grading for Lumbar Facet Joint Degeneration, A Reliability Study","authors":"Xiaowen Liu,&nbsp;Zhenyu Zhu,&nbsp;Shouyu He,&nbsp;Mingjian Sun,&nbsp;Tianyi Zhao,&nbsp;Lei Liu,&nbsp;Haoyang Shi,&nbsp;Yang Hou,&nbsp;Guodong Shi","doi":"10.1002/jsp2.70035","DOIUrl":"10.1002/jsp2.70035","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Lumbar facet joint diseases can often lead to reduced work efficiency and increased medical costs. As a primary imaging tool in orthopedics, X-rays offer numerous advantages. However, there is no consensus on the classification of lumbar facet joints based on X-ray imaging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study was conducted for 356 patients between November 2017 and September 2023. A senior radiologist and a senior orthopedic surgeon discussed and established a grading system for lumbar facet joints based on both X-ray and MRI findings. Two double-blind attending spinal surgeons were asked to evaluate and grade the included cases. The intra-rater reliability and inter-rater reliability were evaluated by assessing the weighted kappa (κw) coefficient.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The level of inter-rater reliability of MRI for complete agreement was good for <i>Doctor A</i> (κw = 0.792) and <i>Doctor B</i> (κw = 0.869). The inter-rater reliability coefficient for grading lumbar facet joint degeneration based on the radiograph was as follows: for <i>Doctor A</i> (κw = 0.702, session 1; κw = 0.847, session 2) and <i>Doctor B</i> (κw = 0.714, session 1; κw = 0.828, session 2). Moreover, the level of intra-rater reliability based on X-ray for complete agreement was excellent for <i>Doctor A</i> (κw = 0.843) and <i>Doctor B</i> (κw = 0.836).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We propose a new grading system for lumbar facet joint degeneration based on X-rays, which serves as a supplement to the Weishaupt and Pathria classifications. This grading system aims to provide clinicians with a more comprehensive understanding of lumbar facet joint degeneration, allowing for the use of a broader range of diagnostic tools to evaluate facet joint degeneration from multiple perspectives. We believe that this grading system can be valuable in assessing potential anatomical changes related to the facet joint, thereby informing modifications to surgical techniques and procedural steps.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Posterior Ligamentum Complex Preservation Alleviate ASD-Related Biomechanical Deterioration in Lumbar Interbody Fusion Models: A Finite Element Analysis","authors":"Lipeng He,&nbsp;Tingchen Zhu,&nbsp;Weiye Cai,&nbsp;Wenhao Yang,&nbsp;Zan Chen,&nbsp;Jingchi Li","doi":"10.1002/jsp2.70030","DOIUrl":"10.1002/jsp2.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>There are differences in the extent of excision of articular processes, spinal processes and posterior ligamentum complexes (PLC) for posterior approach lumbar interbody fusion. Given that the biomechanical significance of these structures has been verified and that deterioration of the biomechanical environment is the main trigger for complications in both fused and adjacent motion segments, changes in decompression ranges may affect the potential risk of adjacent segmental disease (ASD) biomechanically; however, this topic has yet to be identified.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Posterior lumbar interbody fusion (PLIF) with different decompression strategies was simulated in a well-validated lumbosacral model. The excision and preservation of the cranial motion of the segmental PLC and the lateral articular process in the fusion segment were simulated in this model. The stress distribution in the cranial motion segment was computed under different loading conditions to determine the potential risk of ASD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared to complete bilateral articular process excision, preservation of the lateral two-thirds of the articular process did not alleviate stress concentration on the cranial motion segment both in PLC preserved and excised models. In contrast, preservation of the cranial segmental PLC can obviously alleviate the stress concentration tendency of the cranial intervertebral disc under flexion loading conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Preservation of the lateral parts of the articular process cannot optimize the biomechanical environment, in contrast, PLC preservation can effectively alleviate ASD related biomechanical deterioration of the cranium segment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"8 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and ex vivo screening of microRNA combinations with enhanced cell penetrating peptides to stimulate intervertebral disc regeneration","authors":"Tara Ní Néill,&nbsp;Marcos N. Barcellona,&nbsp;Niamh Wilson,&nbsp;Fergal J. O'Brien,&nbsp;James E. Dixon,&nbsp;Caroline M. Curtin,&nbsp;Conor T. Buckley","doi":"10.1002/jsp2.1366","DOIUrl":"10.1002/jsp2.1366","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Low back pain (LBP) is predominantly caused by degeneration of the intervertebral disc (IVD) and central nucleus pulposus (NP) region. Conservative treatments fail to restore disc function, motivating the exploration of nucleic acid therapies, such as the use of microRNAs (miRNAs). miRNAs have the potential to modulate expression of discogenic factors, while silencing the catabolic cascade associated with degeneration. To deliver these miRNAs, nonviral cell penetrating peptides (CPPs) are gaining favor given their low immunogenicity and strong targeting ability. Single miRNA therapies have been investigated for IVD repair, however dual miRNA delivery strategies have not been commonly examined and may augment regeneration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and methods</h3>\u0000 \u0000 <p>Transfection of four pro-discogenic miRNAs (miRNA mimics:140-5p; 149-5p and inhibitors: 141-3p; 221-3p) and dual delivery of six miRNA pairings was performed using two CPPs, RALA and GET peptide (FLR), in primary rat NP monolayer culture, and in an ex vivo organ culture model of rat caudal discs. Protein expression of discogenic (aggrecan, collagen type II, and SOX9) and catabolic markers (ADAMTS5 and MMP13) were assessed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Monolayer investigations signified enhanced discogenic marker expression following dual miRNA delivery, signifying a synergistic effect when compared to single miRNA transfection. Utilization of an appropriate model was emphasized in our ex vivo organ culture experiment, revealing the establishment of a regenerative microenvironment characterized by reduced catabolic enzyme activity and enhanced matrix deposition, particularly following concurrent delivery of FLR-miRNA-149-5p mimic and miRNA-221-3p inhibitor. Bioinformatics analysis of miRNA-149-5p mimic and miRNA-221-3p inhibitor identified distinct targets, pathways, and interactions, suggesting a mode of action for this amplified response.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings suggest the potential of FLR-miRNA-149-5p + miRNA-221-3p inhibitor to create an anti-catabolic niche within the disc to foster regeneration in moderate cases of disc degeneration, which could be utilized in further studies with the overarching aim of developing treatments for LBP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"7 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to improve the mechanical safety of a novel spinal implant while saving costs and time","authors":"Annette Kienle,&nbsp;Hans-Joachim Wilke,&nbsp;Christian Schröder,&nbsp;Andrea Pietsch","doi":"10.1002/jsp2.70026","DOIUrl":"10.1002/jsp2.70026","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Spinal implant failure is associated with prolonged patient suffering, high costs for the medical device industry, and a high economic burden for the health care system. Pre-clinical mechanical testing has great potential to reduce the risk of such failure. However, there are no binding regulations for planning and interpretation of mechanical testing. Therefore, different strategies exist. Mainly for novel implants an option is to start with a structured scientific literature search that forms an objective background for the definition of an implant-specific test plan, the derivation of acceptance criteria and interpretation of the test results.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This paper describes, how a literature-based approach can look like from the initial literature search through the derivation of the test plan and the acceptance criteria, to the final test result evaluation and how this approach can support the proof that the device meets all necessary safety and performance standards.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The main advantage of this literature-based approach is that testing and test result interpretation are linked with the loads acting on the individual implant in vivo. In an ideal case, testing is focused on the individual implant in a way that ensures maximum efficiency during the development and approval process combined with maximum insight in safety and effectiveness of the implant. Even comparative implant testing may become obsolete, which is a big advantage if comparative implant and related data are not available.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This approach to pre-clinical mechanical testing offers the potential to create a chain of arguments, from literature review through testing to the interpretation of test results. This methodology can significantly enhance testing efficiency, reduce risk of failure, and ultimately prevent unnecessary patient suffering and healthcare costs. By synthesizing scientific insights with regulatory requirements, this review aims to guide clinicians and researchers in improving patient care and advancing device technologies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"7 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}