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Immunochromatography of fusarochromanone mycotoxins. 褐黄酮真菌毒素的免疫层析。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01 DOI: 10.1093/JAOAC/74.4.655
J. H. Yu, F. Chu
{"title":"Immunochromatography of fusarochromanone mycotoxins.","authors":"J. H. Yu, F. Chu","doi":"10.1093/JAOAC/74.4.655","DOIUrl":"https://doi.org/10.1093/JAOAC/74.4.655","url":null,"abstract":"The present paper describes an enzyme-linked immunoassay (ELISA) used in combination with thin-layer chromatography (TLC) and liquid chromatography (LC) for determination of fusarochromanone (TDP) mycotoxins in barley, wheat, and a Fusarium culture grown in rice and corn. The mycotoxins were first extracted from the sample with 100% methanol and subjected to TLC or LC without additional cleanup treatment. Individual fractions eluted from TLC or LC were acetylated, then analyzed by ELISA. Determinations of TDP toxins at levels as low as 0.1 and 0.5 ng were achieved by ELISA in combination with LC and TLC, respectively. The detection limit for TDP-1 in barley and wheat was about 20 ppb by ELISA alone as compared with a detection limit of 5 ppb by a combination of ELISA with either TLC or LC. Overall analytical recovery (% of added) of TDP-1 added to barley and wheat at 5, 10, and 20 ppb of TDP-1 was 106.9 +/- 15.3 and 113.2 +/- 11.6 by LC-ELISA and 108.8 +/- 9.1 and 110.4 +/- 4.9 by TLC-ELISA, respectively. Analysis of extracts obtained from Fusarium equiseti R6137 grown in corn and rice by the combination of TLC and ELISA revealed that diacetyl-TDP was also produced by this fungus in addition to TDP-1 and TDP-2. Comparable results were obtained when fungal extracts were subjected to ELISA, LC, and immunochromatography (i.e., combination of ELISA with either TLC or LC).","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"1 1","pages":"655-60"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74563562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
A qualitative colorimetric test for brominated vegetable oil in soft drinks. 软饮料中溴化植物油的定性比色试验。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01 DOI: 10.1093/JAOAC/74.4.698
M. N. Krishna Murthy, S. Rajalakshmi, J. A. Satyabodha, K. Nagaraja
{"title":"A qualitative colorimetric test for brominated vegetable oil in soft drinks.","authors":"M. N. Krishna Murthy, S. Rajalakshmi, J. A. Satyabodha, K. Nagaraja","doi":"10.1093/JAOAC/74.4.698","DOIUrl":"https://doi.org/10.1093/JAOAC/74.4.698","url":null,"abstract":"A simple and precise method of detecting brominated vegetable oil (BVO) in soft drinks is described. After extraction of BVO using diethyl ether, the concentrated ethereal solution was treated with a small quantity of zinc dust to convert the organic bromide to inorganic form; the solution was subsequently treated with lead dioxide to liberate bromine. The bromine evolved was detected by means of fluorescein-impregnated filter paper strip that turns pink because eosin is formed. The test can detect as low as 10 ppm (2 mg/200 ml) of BVO under experimental conditions. Gas chromatography was carried out on sodium methoxide derivatives prepared from ether extract for quantitation.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"21 1","pages":"698-9"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80554203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Rapid pentachlorophenol evaluation in solid matrixes by second derivative UV spectroscopy for application to wood and leather samples. 固体基质中五氯酚的二阶导数紫外光谱快速评价方法在木材和皮革样品中的应用。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
M Secchieri, C A Benassi, S Pastore, A Semenzato, A Bettero, M Levorato, A Guerrato
{"title":"Rapid pentachlorophenol evaluation in solid matrixes by second derivative UV spectroscopy for application to wood and leather samples.","authors":"M Secchieri,&nbsp;C A Benassi,&nbsp;S Pastore,&nbsp;A Semenzato,&nbsp;A Bettero,&nbsp;M Levorato,&nbsp;A Guerrato","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for the quail-quantitative evaluation of pentachlorophenol (PCP) in solid matrixes has been developed. The procedure is based on solid-liquid extraction of solid samples (leather or wood), followed by purification on a cyanopropyl column and determination of the preservative by second derivative UV spectroscopy considering the PCP A peak-through value (304-297 nm). The method allows rapid PCP determination in the concentration range 1-40 micrograms/mL; any matrix interference is avoided by the purification step and recoveries of the preservative were 99.12% (RSD% 0.13) for the leather matrix and 98.03 (RSD% 0.17) for the wood matrix.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"674-8"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13076944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence of an unusual pattern of polychlorinated biphenyls in the serum of some residents and canines in Paoli, Pennsylvania. 在宾夕法尼亚州保利的一些居民和犬的血清中发现了不寻常的多氯联苯模式的证据。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
V W Burse, D F Groce, M P Korver, S P Caudill, P C McClure, C R Lapeza, S L Head, R J Schilling, J A Farrar, S R Ostrowski
{"title":"Evidence of an unusual pattern of polychlorinated biphenyls in the serum of some residents and canines in Paoli, Pennsylvania.","authors":"V W Burse,&nbsp;D F Groce,&nbsp;M P Korver,&nbsp;S P Caudill,&nbsp;P C McClure,&nbsp;C R Lapeza,&nbsp;S L Head,&nbsp;R J Schilling,&nbsp;J A Farrar,&nbsp;S R Ostrowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present study uses gas liquid chromatography (GLC) electron capture detection with packed and capillary columns to detect polychlorinated biphenyls (PCBs) in serum samples from people living near the electric car repair and maintenance facility of the Southeastern Pennsylvania Transit Authority in Paoli, Pennsylvania. Most of the cohort surveyed had serum patterns similar to patterns for Aroclor 1260 (AR 1260); a small portion (3/89) had patterns indicative of an AR with higher chlorination (e.g., AR 1268). In addition to analyzing serum samples from humans, we also analyzed serum samples from canines (pets of some of the subjects). In general, the serum pattern for canines was less descriptive for AR 1260 than the pattern for humans; however, the pattern for several canines (9/16) was that of the higher chlorinated PCBs (e.g., AR 1268). By using mass spectrometry and capillary column GLC, we confirmed the presence of high molecular weight polychlorinated congeners in both human and animal samples. We were not able to show a statistically significant relationship between serum patterns of PCBs in canines and their owners or between canines and certain behavioral traits (e.g., runs free, retrieves, hours outside, hours inside). However, the correlation between PCBs quantified as AR 1268 and canines' residence time was statistically significant.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"577-86"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13078269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modified gas chromatographic/mass spectrometric method for determination of daminozide in high protein food products. 改进气相色谱/质谱法测定高蛋白食品中胺酰肼。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01 DOI: 10.1093/JAOAC/74.4.682
K. Faughnan, M. Woodruff
{"title":"Modified gas chromatographic/mass spectrometric method for determination of daminozide in high protein food products.","authors":"K. Faughnan, M. Woodruff","doi":"10.1093/JAOAC/74.4.682","DOIUrl":"https://doi.org/10.1093/JAOAC/74.4.682","url":null,"abstract":"A modified version of the Conditt and Baumgardner gas chromatographic/mass spectroscopic (GC/MS) method for determination of daminozide in peanut butter and raw peanuts is described. Daminozide in the food product is hydrolyzed to unsymmetrical dimethylhydrazine (UDMH) by sodium hydroxide digestion. The generated UDMH is distilled from the food matrix and captured by reaction with salicylaldehyde in a condensation trap. Resulting high pH distillates generated by peanuts and peanut products are adjusted back to a pH of 5-6 through addition of glacial acetic acid. After thermal incubation and extraction into methylene chloride, salicylaldehyde dimethylhydrazone is separated from interferences by capillary GC and quantitated by MS using the selective ion monitoring (SIM) mode. Quantitation of daminozide is based on the ratio of the salicylaldehyde dimethylhydrazone molecular ion (m/z 164) to the molecular ion (m/z 153) of the internal standard, 4-nitroanisole. Confirmation of daminozide identity is determined by relative intensity of the m/z 164 ion to the m/z 120 (C7H4ON) ion. Improved m/z 164 ion intensity and reduction of neighboring interferences due to acetic acid treatment permitted a daminozide detection limit of 0.005 ppm in a 50 g sample and an associated 0.02 ppm limit of quantitation. This modification is specific for high protein samples that generate high pH distillates such as peanuts and peanut products and is not specifically intended for analysis of low protein samples.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"18 1","pages":"682-92"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82186635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Lipid globule staining to aid in differentiating Bacillus species. 脂球染色帮助区分芽孢杆菌种类。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01 DOI: 10.1093/JAOAC/74.4.649
S. Harmon, D. Kautter, G. Lancette
{"title":"Lipid globule staining to aid in differentiating Bacillus species.","authors":"S. Harmon, D. Kautter, G. Lancette","doi":"10.1093/JAOAC/74.4.649","DOIUrl":"https://doi.org/10.1093/JAOAC/74.4.649","url":null,"abstract":"The use of the lipid globule stain to aid in differentiating the Bacillus cereus group (i.e., B. cereus, B. cereus var. mycoides, and B. thuringiensis) from other Bacillus species was investigated. Smears from colonies grown on suitable agar were made on precleaned slides, stained, and examined microscopically for characteristic deep blue lipid globules. The study included a total of 649 cultures of Bacillus species plus 143 incompletely characterized Bacillus isolates from food. Only B. cereus, B. cereus var. mycoides, B. thuringiensis, B. megaterium, and B. sphaericus were consistently positive for lipid globules, although at times, a few cells of B. aneurinolyticus and B. thiaminolyticus were also positive. The lipid globule stain procedure is of value in differentiating Bacillus species, especially when performed by an experienced analyst and used in conjunction with tests for cell and spore morphology.","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"23 1","pages":"649-51"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84491090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Symposium on microbiology update: old friends and new enemies. Bacillus cereus. 微生物学研讨会最新进展:老朋友和新敌人。蜡样芽胞杆菌。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
S G Jackson
{"title":"Symposium on microbiology update: old friends and new enemies. Bacillus cereus.","authors":"S G Jackson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacillus cereus is an environmentally ubiquitous, Gram-positive, spore-forming bacillus responsible for 2 distinct foodborne disease syndromes as well as other manifestations of pathogenicity. The rapid-onset, \"emetic,\" foodborne-disease syndrome is associated with an emetic toxin; the delayed-onset, \"diarrheal\" syndrome is associated with elaboration of enterotoxin. The majority of methods for detection of these toxins have relied on in vivo testing. More recent work on purification of enterotoxin facilitated the development of a rapid, specific, fluorescent immunodot assay and a tissue culture screening assay for enterotoxin. Work on characterization and detection of emetic toxin is ongoing.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"704-6"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13076878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of oxolinic acid residues in salmon muscle tissue by liquid chromatography with fluorescence detection. 荧光液相色谱法测定鲑鱼肌肉组织中氧喹啉酸残留量。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
L Larocque, M Schnurr, S Sved, A Weninger
{"title":"Determination of oxolinic acid residues in salmon muscle tissue by liquid chromatography with fluorescence detection.","authors":"L Larocque,&nbsp;M Schnurr,&nbsp;S Sved,&nbsp;A Weninger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present paper describes a method for determination of oxolinic acid in salmon muscle tissue. Tissue (0.5-2 g) mixed with 2 g anhydrous sodium sulfate is extracted twice with ethyl acetate, centrifuged, and the extract evaporated. The residue is partitioned in a mixture of hexane and 0.01M oxalic acid and the aqueous phase chromatographed using fluorescence detection at 327 nm excitation and 369 nm emission. Calibration and standard curves are linear from 10-200 ppb and 100-2000 ppb at different sensitivity settings. Recoveries ranged from 71-83% in spiked blanks, with a CV of 4-10.3% over a 2-week period. Preliminary results in treated salmon were variable, possibly because some fish refused to eat medicated feed.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"608-11"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13078272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liquid chromatographic determination of tolnaftate in commercial products. 液相色谱法测定商品中苯甲酸酯的含量。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
R D Thompson, M Carlson
{"title":"Liquid chromatographic determination of tolnaftate in commercial products.","authors":"R D Thompson,&nbsp;M Carlson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A liquid chromatographic (LC) method for the determination of the antifungal agent tolnaftate was developed. Isolation of the analyte was achieved by direct extraction or dilution with acetonitrile-water (80 + 20) followed by reverse-phase liquid chromatography using a C18 column. The mobile phase was acetonitrile-water (80 + 20) acidified with phosphoric acid. Detection was by UV absorption at a wavelength of 257 nm. The proposed procedure was applied to 20 consumer products comprising 6 formulation types, including solutions, powders, liquid and power aerosols, creams, and gels. The precision (RSD) for the products ranged from 0.23 to 1.16% (n = 5), and recoveries via fortification ranged from 98.1 to 103.0%. Six different brands of C18 columns were evaluated for use with the method. The overall simplicity and versatility of the method suggest possible adaptations to both regulatory and quality-control situations.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"603-7"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13078274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of Preston agar and a blood-free selective medium for detection of Campylobacter jejuni in food. 普雷斯顿琼脂与无血选择培养基检测食品中空肠弯曲杆菌的比较。
Journal - Association of Official Analytical Chemists Pub Date : 1991-07-01
M Peterz
{"title":"Comparison of Preston agar and a blood-free selective medium for detection of Campylobacter jejuni in food.","authors":"M Peterz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present collaborative study compares recovery of Campylobacter jejuni from food in 2 agar media. Six laboratories analyzed 8 samples each of chicken liver inoculated with Campylobacter jejuni. Samples were enriched in Preston broth and isolation was carried out on Preston agar (PA) and campylobacter blood-free selective medium (CBFS), a charcoal-based medium with cefoperazone and amphoteracin as antibiotic supplements. There was no difference in the recovery rate between the 2 agar media; however, the specificity of CBFS was better than that of PA. There was a slightly better growth of campylobacters, and competing organisms were more inhibited on CBFS than on PA.</p>","PeriodicalId":14752,"journal":{"name":"Journal - Association of Official Analytical Chemists","volume":"74 4","pages":"651-4"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13078276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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