S. Vaid, P. Srivastava, S. P. Pachauri, Anita Sharma, D. Rawat, Shailesh Chandra Shankhadhar, A. Shukla
{"title":"Effective zinc mobilization to rice grains using rhizobacterial consortium","authors":"S. Vaid, P. Srivastava, S. P. Pachauri, Anita Sharma, D. Rawat, Shailesh Chandra Shankhadhar, A. Shukla","doi":"10.1163/22238980-20201085","DOIUrl":"https://doi.org/10.1163/22238980-20201085","url":null,"abstract":"Low Zn in staple food grains like rice is closely related to large scale Zn malnutrition in many countries of the World. Zinc biofortification of rice grains by some cost effective agronomic method is important for low income farmers. To explore the possibility of enhancing the bioavailability of Zn in rice grains besides higher yields of two cultivars, the combinations of varying Zn fertilizer doses with or without inoculation of rhizobacteria consortium under split plot design set up were evaluated in two years field trials. Microbial inoculation + 5 kg Zn ha-1 to I year rice crop resulted in the highest number of effective tillers, grain yields, Zn concentration and uptake in grains and straw and total Zn uptake in both years. Grain yield of rice during two years increased by 19.7-27.9 and 17.1-20.4 percent over control under treatments receiving microbial inoculation + 5 kg Zn ha-1 to I year rice and 5 kg Zn ha-1 alone to I year rice crop, respectively. The highest concentration of Zn (10.9-19.1 mg kg-1) and the lowest concentration of phytic acid (18.5-25.3 g kg-1) in dehulled rice grains were recorded with soil application of 5 kg Zn ha-1; however, the values were at par with those observed under microbial inoculation + 5 kg Zn ha-1 (12.0-17.0 mg Zn kg-1 and 19.2-26.9 g phytic acid kg-1). The percent utilization of soil applied Zn increased with microbial inoculation in both the years and it was relatively higher in NDR 359 as compared to PD 16.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":" ","pages":"1-13"},"PeriodicalIF":1.0,"publicationDate":"2020-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44201098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of bacterial consortium on alfalfa (Medicago sativa L.) plant nutrient uptake and antioxidant enzymes at different levels of oily sludge","authors":"A. Shahzad, A. Bano, S. Siddiqui","doi":"10.1163/22238980-20201078","DOIUrl":"https://doi.org/10.1163/22238980-20201078","url":null,"abstract":"Under stress environment of oily sludge, plants develop oxidative stress which effect nutrients uptake, activity of oxidative defence enzymes, cause ion imbalance and toxicity in plants. The present study was designed to develop a combination of bacterial consortium alone and with fertilizers that can help to improve alfalfa growth and plant defence system under stress environment of oily sludge contamination soils. For this study consortium was prepared from Bacillus cereus (Acc KR232400), Bacillus altitudinis (Acc KF859970), Comamonas (Acc KF859971) and Stenotrophomonas maltophilia (Acc KF859973) and was inoculated with fertilizer to oily sludge contaminated soils. A pot experiment was conducted using complete randomized design with three replicates. The plants were harvested at 21 d for estimation of protein, proline and antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD). The protein, SOD and POD contents in alfalfa were higher in oily sludge than soil without consortium, ammonium nitrate and diamamoiun phosphate. Consortium suppressed the oxidative stress of sludge treated plants. The inoculation of bacterial consortium enhanced the uptake of Ca, Mg, K and Na. The uptake of Ca, Mg, K, Fe, Cu and Zn increased significantly with consortium+fertilizer the availability of nutrients in soil with 30% and 60% of oily. Cd content was greater in root than leaves of alfalfa. The bacterial consortium helped to enhance plant growth and plant anti-oxidant enzymes system. The consortium with fertilizer is the best suitable combination for alfalfa that can improve the oxidative enzyme system of alfalfa and increases its growth and development.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":" ","pages":"1-13"},"PeriodicalIF":1.0,"publicationDate":"2020-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46217035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Avi Eliyahu, Zvi Duman, S. Sherf, O. Genin, Y. Cinnamon, M. Abu-Abied, Roy Weinstain, A. Dag, E. Sadot
{"title":"Vegetative propagation of elite Eucalyptus clones as food source for honeybees (Apis mellifera); adventitious roots versus callus formation","authors":"Avi Eliyahu, Zvi Duman, S. Sherf, O. Genin, Y. Cinnamon, M. Abu-Abied, Roy Weinstain, A. Dag, E. Sadot","doi":"10.1163/22238980-20191112","DOIUrl":"https://doi.org/10.1163/22238980-20191112","url":null,"abstract":"Summer and autumn in Israel are highly arid with not enough plants in bloom offering nectar and pollen to support the local apiary. This leads to decline in colony health and honey production. To increase food sources for honeybees, we initiated a project to clone elite Eucalyptus trees exhibiting constant and rich blooming from late summer to early winter. We induced adventitious roots from cuttings of two mature Eucalyptus trees of which nectar production and honeybees’ attraction was measured: Eucalyptus brachyphylla and Eucalyptus x trabutii. During the rooting process, a high frequency of cylindrical callus formation instead of roots was obtained. To shed light on the inner anatomy of the callus chunks, we compared their cell organization and cell-wall composition to those of roots. Whereas in the root, cells were organized in circumferential symmetry, no symmetry was found in the callus. Instead, a more chaotic accumulation of meristematic-like cells with sporadic clusters of tracheary elements laid in different directions were observed. The outer cell layer of the callus often included swollen cells with thin cell walls. Most callus cells stained more strongly for cellulose and lignin than cells in the root meristem. In addition, specific antibodies to methylesterified and de-methylesterified pectin showed differential staining of callus vs. root cells indicating cell wall differences. Strikingly, roots were seen to differentiate from the chaotic cell organization of the callus, albeit at low rates. Further investigation of the cellular and molecular mechanisms underlying callus formation, are required.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46286098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Verstraeten, H. Buyle, S. Werbrouck, M. V. Labeke, D. Geelen
{"title":"In vitro shoot growth and adventitious rooting of Wikstroemia gemmata depends on light quality","authors":"I. Verstraeten, H. Buyle, S. Werbrouck, M. V. Labeke, D. Geelen","doi":"10.1163/22238980-20191110","DOIUrl":"https://doi.org/10.1163/22238980-20191110","url":null,"abstract":"In vitro propagation of the ornamentally interesting species Wikstroemia gemmata is limited by the recalcitrance to form adventitious roots. In this article, two strategies to improve the rooting capacity of in vitro microcuttings are presented. Firstly, the effect of exogenous auxin was evaluated in both light and dark cultivated stem segments and also the sucrose-content of the medium was varied in order to determine better rooting conditions. Secondly, different spectral lights were evaluated and the effect on shoot growth and root induction demonstrated that the exact spectral composition of light is important for successful in vitro growth and development of Wikstroemia gemmata. We show that exogenous auxin cannot compensate for the poor rooting under unfavorable light conditions. Adapting the culture conditions is therefore paramount for successful industrial propagation of Wikstroemia gemmata.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"67 1","pages":"16-26"},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42221418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Valladares, E. Varas, J. Vielba, N. Vidal, V. Codesido, R. Castro, C. Sánchez
{"title":"Expression of a Rap2.12 like-1 ERF gene during adventitious rooting of chestnut and oak microshoots","authors":"S. Valladares, E. Varas, J. Vielba, N. Vidal, V. Codesido, R. Castro, C. Sánchez","doi":"10.1163/22238980-20191119","DOIUrl":"https://doi.org/10.1163/22238980-20191119","url":null,"abstract":"Adventitious rooting of cuttings is a complex developmental process in forest species, with several exogenous and endogenous factors influencing the outcome of the process. In this study we applied an in vitro working system, comprising two lines of microshoots with the same genotype but at a different ontogenetic stages, in two different tree species (chestnut and oak). We analyzed the expression of a gene encoding an AP2/ERF transcription factor from group VII in the initial hours of the adventitious rooting induction, both in rooting competent and incompetent microshoots. The analysis revealed that expression of this gene is related to wounding, ontogenetic stage and auxin in a complex and species-specific manner. Putative induction of the gene by auxin was also analyzed in the presence of naphthyl-phthalamic acid (NPA), an auxin transport inhibitor. In situ expression analysis in chestnut relates the gene activity to cambial divisions and root primordia in rooting competent microshoots, as well as in the root apex. The putative role of the gene during adventitious roots formation is discussed.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44622908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Johnatan Vilasboa, Cibele Tesser da Costa, H. Matsuura, A. Fett-Neto
{"title":"Rooting of cuttings of Passiflora suberosa, a medicinal passion fruit species: characterization and modulation by external biochemical factors","authors":"Johnatan Vilasboa, Cibele Tesser da Costa, H. Matsuura, A. Fett-Neto","doi":"10.1163/22238980-20191114","DOIUrl":"https://doi.org/10.1163/22238980-20191114","url":null,"abstract":"\u0000Passiflora suberosa L. (Passifloraceae) can be found throughout the Americas, and has several medicinal properties, including antioxidant, antibacterial, anti-hemolytic, hypolipidemic, and hypoglycemic activities. Germination rates of P. suberosa are low, even with dormancy breaking treatments, posing an obstacle for its multiplication. Vegetative propagation is a valuable approach to produce clones of elite individuals with important pharmacological characteristics, affording fast genetic improvement of biomass source for both phytomedicine manufacturing and bioactive compound isolation. Understanding the rooting process of this species is an important step to exploit its full potential in a sustainable way. We investigated adventitious rooting (AR) in absence or presence of exogenous auxin in P. suberosa cuttings, using a non-aerated hydroponic system. Changes in concentration of flavonoids, phenolics, hexoses, starch, and auxin, as well as peroxidase activity, were monitored along AR. Cuttings showed spontaneous rooting, although the application of exogenous indole-3-butyric acid (IBA) yielded higher number of shorter roots. Biochemical parameters, mainly concentration of carbohydrates and total phenolics, as well as peroxidase activity, varied along the course of the experiments. Based on these results, attempts were made to up- or down-modulate rooting responses by applying putative regulators to the growth solution at different time points. It was possible to block the positive effect of auxin on root development, with only minor positive impacts on the modulated control devoid of auxin. Overall analyses suggested that the rooting system proved effective and specific peroxidase activity showed partial correlation with AR, being able to suffer modulation by culture solution factors.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"67 1","pages":"40-51"},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43256431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of polar auxin transport and gibberellins on xylem formation in pine cuttings under adventitious rooting conditions","authors":"A. Pizarro, C. Díaz-Sala","doi":"10.1163/22238980-20191120","DOIUrl":"https://doi.org/10.1163/22238980-20191120","url":null,"abstract":"Maturation-related decline of adventitious root formation is one of the major factors affecting adventitious rooting in forest tree species. We demonstrate that inhibition of polar auxin transport promoted cambium and xylem differentiation in rooting-competent hypocotyl cuttings from Pinus radiata under conditions of adventitious root formation. Treatments with bioactive gibberellins inhibited rooting while at the same time inducing both the differentiation of a continuous ring of cambium and xylem formation. Treatments with inhibitors of gibberellin biosynthesis did not affect the rooting response. The results demonstrate that xylem parenchyma and procambial cells at the xylem poles of rooting-competent hypocotyl cuttings after excision and under conditions of adventitious root induction become adventitious root meristems or xylem, depending on the directional auxin flow. Gibberellin may interact with this pathway, inducing xylem differentiation and inhibiting rooting. We conclude that modifications of auxin flow at the rooting sites, and the priming of cambial cells to differentiate into xylem during tree ageing, may be associated with the maturation-related decline of adventitious root formation.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"67 1","pages":"27-39"},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49270834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Vielba, N. Vidal, A. Ricci, R. Castro, Purificación Covelo, M. C. San-José, C. Sánchez
{"title":"Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots","authors":"J. Vielba, N. Vidal, A. Ricci, R. Castro, Purificación Covelo, M. C. San-José, C. Sánchez","doi":"10.1163/22238980-20191113","DOIUrl":"https://doi.org/10.1163/22238980-20191113","url":null,"abstract":"The present study investigated how auxin concentration and the method of application affected the formation of adventitious roots in microshoots of chestnut (Castanea sativa) and oak (Quercus robur). The activity of two urea derivatives (2, 3-MDPU and 3, 4-MDPU) was also evaluated. Microshoots were derived from basal sprouts of two mature chestnut trees (P1 and P2) and one adult oak genotype (Sainza). In chestnut, rooting percentage was positively affected by auxin in a dose -dependent manner, particularly in shoots treated with the hormone for 24 h. The effect of auxin on rooting also differed depending on the application method. In shoots treated for 24 h, the highest concentration of auxin produced the healthiest rooted plantlets, in terms of the root system and shoot quality. By contrast, in shoots treated by the basal quick-dip method, the shoot quality was best at the lowest auxin concentration. The effect of urea derivatives on the root system depended on the species as well as on the auxin concentration and application period. Use of the MDPUs improved the root system architecture of auxintreated shoots by promoting lateral root development and triggering the synchronous initiation of root primordia at the base of the shoot. Shoot quality was also improved by MDPUs, which promoted resumption of growth and reduced shoot-tip necrosis.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"67 1","pages":"52-68"},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42616126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Control of root system architecture by phytohormones and environmental signals in rice","authors":"Chen Lin, M. Sauter","doi":"10.1163/22238980-20191108","DOIUrl":"https://doi.org/10.1163/22238980-20191108","url":null,"abstract":"Drought and flooding are environmental extremes and major threats to crop production. Water uptake is achieved by plant roots which have to explore new soil spaces to alleviate water deficit during drought or to cope with water excess during flooding. Adaptation of the root system architecture helps plants cope with such extreme conditions and is crucial for plant health and survival. While for dicot plants the well studied model plant Arabidopsis thaliana has provided insight into the genetic and molecular regulation of the root system, less information is available for monocot species, which include the agronomically important cereal crops. Rice (Oryza sativa L.) is a semi-aquatic monocot plant that develops strong tolerance to flooding. Flooding tolerance of rice is closely linked to its adaptive root system. The functional root system of rice is mainly composed of crown roots and is shifted to nodal adventitious roots during flooding which allows rice to maintain oxygen supply to the roots and to survive longer periods of partial submergence as compared with other crops. Likewise, a number of drought-tolerance traits of rice are the result of an altered root system architecture. Hence, the structure of the root system adapts to, both, flooding and drought. Understanding the regulatory mechanisms that control root system adaptation to extreme environments is a key task for scientists to accelerate the breeding efforts for stress-tolerant crops. This review summarizes recently identified genes and molecular mechanisms that regulate root system architecture in rice in response to drought and flooding.","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20191108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42438118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dedication to Professor Joseph Riov","authors":"S. Philosoph-Hadas","doi":"10.1163/22238980-20190002","DOIUrl":"https://doi.org/10.1163/22238980-20190002","url":null,"abstract":"","PeriodicalId":14689,"journal":{"name":"Israel Journal of Plant Sciences","volume":"67 1","pages":"3-4"},"PeriodicalIF":1.0,"publicationDate":"2020-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1163/22238980-20190002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42099713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}