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Iraqi journal of Veterinary Sciences最新文献

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Isolation, characterization, and ELISA applications of alkaline phosphatase and acetylcholinesterase from Moniezia expansa 扩张莫尼茨绦虫碱性磷酸酶和乙酰胆碱酯酶的分离、表征和酶联免疫吸附试验的应用
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.142183.3161
D. A. Darwish, H. Masoud, M. S. Helmy, W. T. Abbas, R. Shaapan, N. Toaleb, M. A. Ibrahim
{"title":"Isolation, characterization, and ELISA applications of alkaline phosphatase and acetylcholinesterase from Moniezia expansa","authors":"D. A. Darwish, H. Masoud, M. S. Helmy, W. T. Abbas, R. Shaapan, N. Toaleb, M. A. Ibrahim","doi":"10.33899/ijvs.2023.142183.3161","DOIUrl":"https://doi.org/10.33899/ijvs.2023.142183.3161","url":null,"abstract":"Moniezia expansa worms are a significant source of alkaline phosphatase (ALP) and acetylcholinesterase (AChE) enzymes. The current study presents a simple and reproducible ALP and AChE purification method from Moniezia expansa helminthes by precipitating the proteins with ammonium sulfate and chromatography on the Sephacryl S-300 column. The M. expansa ALP purified at 1070.8 U/mg, displaying 6.0 purification folds and 53.6% yield, while M. expansa AChE is at 5250 U/mg, displaying 2.0 purification folds and 43% yield. The M. expansa ALP isoenzyme displayed its optimum activity at pH 9.6, while the M. expansa AChE isoenzyme displayed its optimum activity at pH 8.0. The affinity of M. expansa ALP for several substrates revealed that p-nitrophenyl phosphate preferentially cleaved with a Km value of 4.4 mM. M. expansa AChE preferentially cleaved acetylthiocholine iodide with a Km value of 0.9 mM. M. expansa ALP is strongly stimulated with Co 2+ , Mn 2+ , Ni 2+ , and Mg 2+ and reduced with Zn 2+ , Cu 2+ , Ca 2+ , EDTA and DTT. On the other hand, M. expansa AChE is significantly induced with Co 2+ , Zn 2+ , and Ni 2+ and inhibited with Mg 2+ , Ca 2+ , EDTA, 1,10-phenanthroline and eserine. The antisera of the purified M. expansa ALP and AChE found effective for determining the two enzymes in different unknown sera from different animal species, including humans, sheep and fish. These results may provide a possible future application of such enzymes in producing ALP and AChE-coated ELISA plates for research purposes.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"92 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139124995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Costus-loaded silver nanoparticles mitigated AMPK and related pathways in the albino rat atherosclerosis model 在白化大鼠动脉粥样硬化模型中，负载银纳米粒子的 Costus 可减轻 AMPK 及相关通路的作用
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.140352.3039
Enaam E. Jabori, Hana Kh. Ismail, L. W. Khaleel, Ahmed N. Flaih
{"title":"Costus-loaded silver nanoparticles mitigated AMPK and related pathways in the albino rat atherosclerosis model","authors":"Enaam E. Jabori, Hana Kh. Ismail, L. W. Khaleel, Ahmed N. Flaih","doi":"10.33899/ijvs.2023.140352.3039","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140352.3039","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"86 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The radiological study of using fabricated calcium hydroxide from quail eggshell and plasma-rich fibrin for reconstitution of a mandibular bone gap in dogs 使用从鹌鹑蛋壳中提取的氢氧化钙和富含血浆的纤维蛋白重建狗下颌骨间隙的放射学研究
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.139898.2998
A. G. Atiyah, L. M. Alkattan, A. M. Shareef
{"title":"The radiological study of using fabricated calcium hydroxide from quail eggshell and plasma-rich fibrin for reconstitution of a mandibular bone gap in dogs","authors":"A. G. Atiyah, L. M. Alkattan, A. M. Shareef","doi":"10.33899/ijvs.2023.139898.2998","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139898.2998","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"33 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the advantages of orphenadrine in anaesthesia caused by ketamine in mice 评估氯胺酮麻醉小鼠时奥芬那君的优势
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.141516.3119
Ahmed S. Naser, Y. Albadrany, Manahel A. Abdullah
{"title":"Evaluation of the advantages of orphenadrine in anaesthesia caused by ketamine in mice","authors":"Ahmed S. Naser, Y. Albadrany, Manahel A. Abdullah","doi":"10.33899/ijvs.2023.141516.3119","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141516.3119","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"53 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First analysis of beak and feather disease of psittacine in Nineveh province, Iraq 对伊拉克尼尼微省鹦鹉喙和羽毛疾病的首次分析
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.141118.3104
Ayman A. Al-Alhially
{"title":"First analysis of beak and feather disease of psittacine in Nineveh province, Iraq","authors":"Ayman A. Al-Alhially","doi":"10.33899/ijvs.2023.141118.3104","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141118.3104","url":null,"abstract":"","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"33 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macroscopic and histomorphometry investigation of pancreas in adult local partridge (Francolinus francolinus) 本地成年鹧鸪胰腺的宏观和组织形态学研究
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.142082.3157
K. I. Alkhazraji, R. A. Naser
{"title":"Macroscopic and histomorphometry investigation of pancreas in adult local partridge (Francolinus francolinus)","authors":"K. I. Alkhazraji, R. A. Naser","doi":"10.33899/ijvs.2023.142082.3157","DOIUrl":"https://doi.org/10.33899/ijvs.2023.142082.3157","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"44 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Risk factors assessment and antimicrobial resistance of Salmonella isolates from apparently healthy and diarrheal dogs in Baghdad, Iraq 伊拉克巴格达表面健康的狗和腹泻狗分离出的沙门氏菌的风险因素评估和抗菌药耐药性
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.139817.2984
Fadhaa H. Abdullah, Nagham M. Al-gburi
{"title":"Risk factors assessment and antimicrobial resistance of Salmonella isolates from apparently healthy and diarrheal dogs in Baghdad, Iraq","authors":"Fadhaa H. Abdullah, Nagham M. Al-gburi","doi":"10.33899/ijvs.2023.139817.2984","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139817.2984","url":null,"abstract":"The dog was an essential source of Salmonella ( S. ) transmission. One hundred sixty-five rectal swabs were taken from apparently healthy dogs 90 and diarrheal dogs 75 in Baghdad province, Iraq. The Salmonella species was found using standard bacterial culture, biochemical tests, an analytical profile index API-20, the VITEK2 compact system, and serotyping. Salmonella isolates were screened using the disc diffusion technique for susceptibility to ten antimicrobials. Findings indicated that the Salmonella species prevalence rate was 6.06% from dogs’ fecal samples, with three species identified; the most common serovar was S. Typhimurium, S . Enteritidis, and S . Muenchen, which was isolated for the first time from dogs in Iraq. Statistically significant risk factors concerning the diarrheal state, breed, feeding, and body condition were found. High occurrences were in diarrheal dogs 10.66% compared to non-diarrheic dogs 2.22%; the large dog breed had a higher isolate rate of Salmonella than the small breed. Thin body condition dogs were more at risk than fatty dogs, and the dogs consuming uncooked feed had a higher percentage of Salmonella isolates and were more at risk than those consuming mixed food. Non-significant differences were found according to the gender, age, and educational status of dog owners. Salmonella isolates exhibited high resistance to cefotaxime, ampicillin, azithromycin, gentamycin, and tetracycline but were sensitive to norfloxacin. All the isolates were multidrug-resistant (MDR) except S . Muenchen. In conclusion, diarrheal and apparently healthy dogs carry and shed resistant Salmonella spp., a potential public health risk.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"131 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of potential effects of orphenadrine upon anesthesia with propofol and/or thiopental in mice 检测奥芬那君对小鼠异丙酚和/或硫喷妥类麻醉的潜在影响
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.141780.3135
Y. Albadrany, Ahmed S. Naser, Khalid A. Shaban
{"title":"Detection of potential effects of orphenadrine upon anesthesia with propofol and/or thiopental in mice","authors":"Y. Albadrany, Ahmed S. Naser, Khalid A. Shaban","doi":"10.33899/ijvs.2023.141780.3135","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141780.3135","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"42 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study the biochemical, hematological, and histopathological alteration in heat-stress fish treated with an immune stimulant 研究用免疫兴奋剂处理热应激鱼的生化、血液学和组织病理学变化
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.139185.2906
H.Kh. Ismail, H. B. Al-sabaawy, R. T. Altaee, A. Alsaidya, A. H. Alhamdani
{"title":"Study the biochemical, hematological, and histopathological alteration in heat-stress fish treated with an immune stimulant","authors":"H.Kh. Ismail, H. B. Al-sabaawy, R. T. Altaee, A. Alsaidya, A. H. Alhamdani","doi":"10.33899/ijvs.2023.139185.2906","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139185.2906","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"48 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence, antibiotic resistance, and phylogenetic analysis of Listeria monocytogenes isolated from various sources in Egypt: fish, vegetables, and humans 从埃及鱼类、蔬菜和人类等不同来源分离出的李斯特菌的流行率、抗生素耐药性和系统发育分析
Iraqi journal of Veterinary Sciences Pub Date : 2024-01-01 DOI: 10.33899/ijvs.2023.140242.3033
Sahar O. Saleh, Asmaa A. Hussien, A. Youseef, Waleed K. Younis, A. G. Mubarak
{"title":"Prevalence, antibiotic resistance, and phylogenetic analysis of Listeria monocytogenes isolated from various sources in Egypt: fish, vegetables, and humans","authors":"Sahar O. Saleh, Asmaa A. Hussien, A. Youseef, Waleed K. Younis, A. G. Mubarak","doi":"10.33899/ijvs.2023.140242.3033","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140242.3033","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"93 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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