Sri Lankan Journal of Biology最新文献

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Dietary intake and nutritional anthropometry of the non-academic employees of the University of Sri Jayewardenepura (USJP), Sri Lanka 斯里兰卡Sri Jayewardenepura大学(USJP)非学术雇员的饮食摄入和营养人体测量
Sri Lankan Journal of Biology Pub Date : 2020-06-23 DOI: 10.4038/sljb.v5i2.61
M. Gunathilaka, U. Hettiarachchi
{"title":"Dietary intake and nutritional anthropometry of the non-academic employees of the University of Sri Jayewardenepura (USJP), Sri Lanka","authors":"M. Gunathilaka, U. Hettiarachchi","doi":"10.4038/sljb.v5i2.61","DOIUrl":"https://doi.org/10.4038/sljb.v5i2.61","url":null,"abstract":"Studies on nutritional status of state employees are limited and therefore the present study was conducted to determine the nutritional status of aselected sample of employees attached to the University of Sri Jayewardenepura (USJP), who directly involve in facilitating the academic activities of the University. This is a cross-sectional study carried out by interviewing 130 non-academic members of USJP staff for collecting information on their socio-demographic factors, physical activity levels and food habits. Body Mass Index (BMI) and Waist to Hip ratio (WHR) were calculated according to the WHO anthropometric standards while seven-day food consumption was recorded to assess the food and energy intake in the participants. The prevalence of overweight and obesity in the sample was32.3% and 10.8% respectively while 53.8% and 63.9% of the participants had WC (waist circumference) and WHR. Among the participants, 79.2% of members had a sedentary lifestyle spending most of their time sitting without exercises at work and at home. Moreover, they showed a significant relationship with overweight and obesity (r=0.4, p","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122367798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effects of low-pressure storage of food commodities on the mortality of adult stored product insects 食品类商品低压贮藏对成虫死亡率的影响
Sri Lankan Journal of Biology Pub Date : 2020-01-28 DOI: 10.4038/sljb.v5i1.52
B. Prasantha
{"title":"Effects of low-pressure storage of food commodities on the mortality of adult stored product insects","authors":"B. Prasantha","doi":"10.4038/sljb.v5i1.52","DOIUrl":"https://doi.org/10.4038/sljb.v5i1.52","url":null,"abstract":"The food industry faces serious problems regarding pest infestation leading to high storage loss of food commodities. Due to adverse impacts of chemical preservation methods, a promising physical method of food protection could be considered as an urgent need. This study investigated the effect of low-pressure (vacuum) on the mortality of adults of three stored product pests of drug store beetle (Stegobium paniceum L.), southern cowpea weevil (Callosobruchus chinensis L.) and red flour beetle (Tribolium castaneum Herbst). The pests were exposed to 20 kPa and 10 kPa vacuum at 37 oC and 75% relative humidity. Mortality of the insects was recorded at 8 h and 32 h after vacuum treatments. There were significant differences (p 70% inside the vacuum chamber during treatments.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126285860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Evaluation of microscopy and rapid diagnostic tests for diagnosing malaria among febrile subjects in Ezinihitte local government area, Imo state Nigeria 评估尼日利亚伊莫州Ezinihitte地方政府地区发热受试者中用于诊断疟疾的显微镜和快速诊断试验
Sri Lankan Journal of Biology Pub Date : 2019-07-05 DOI: 10.4038/SLJB.V4I2.40
O. P. Irole-Eze, O. M. Ukpai, B. U. Ejike, C. Nwadike, O. Egwu, N. Okorocha, E. C. Amaechi
{"title":"Evaluation of microscopy and rapid diagnostic tests for diagnosing malaria among febrile subjects in Ezinihitte local government area, Imo state Nigeria","authors":"O. P. Irole-Eze, O. M. Ukpai, B. U. Ejike, C. Nwadike, O. Egwu, N. Okorocha, E. C. Amaechi","doi":"10.4038/SLJB.V4I2.40","DOIUrl":"https://doi.org/10.4038/SLJB.V4I2.40","url":null,"abstract":"Malaria is the leading cause of morbidity and mortality in Nigeria, the country accounting for the highest devastation worldwide. This study aimed at evaluating the performance of microscopy and rapid diagnostic tests (RDTs) for diagnosis of malaria. A total of 443 suspected Plasmodium falciparum infected patients in hospitals in selected communities of Ezinihitte Local Government Area south eastern Nigeria between January and June 2014 were examined by using microscopy and RDTs. Thick and thin blood smears made on clean slides from venipuncture blood collected from febrile subjects have been examined for the presence of malarial parasites. Serological diagnosis was performed using Carestart, SD Bioline and Antec kits. The overall prevalence of the three RDTs were Carestart pf (41.8%), SD Bioline pf (52.8%), and Antec pf/pv (25.3%) as against microscopy the standard (44.9%). The varied prevalence observed from the three kits did not differ statistically (p>0.05).The females appeared to have more infected (42.4%) than the males (39.6%) with no significant difference between the diagnostic methods. The overall sensitivities of Carestart pf, SD Bioline pf and Antec pf/pv were 71.9%, 86.9% and 50.8% respectively while their corresponding specificities were 82.8%, 75.0%, and 95.5% respectively. SD Bioline HRP2 based test demonstrated a superior sensitivity compared to microscopy. Some RDTS can be useful alternatives to microscopy in the diagnosis of P. falciparum especially in resource limited communities.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129413689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of ovitrap surveillance to assess dengue outbreak risks in selected dengue endemic areas in Sri Lanka 在斯里兰卡选定的登革热流行地区使用诱蚊诱卵器监测评估登革热暴发风险
Sri Lankan Journal of Biology Pub Date : 2019-07-05 DOI: 10.4038/SLJB.V4I2.39
M. D. Nirmani, K. Perera, G. Galhena
{"title":"Use of ovitrap surveillance to assess dengue outbreak risks in selected dengue endemic areas in Sri Lanka","authors":"M. D. Nirmani, K. Perera, G. Galhena","doi":"10.4038/SLJB.V4I2.39","DOIUrl":"https://doi.org/10.4038/SLJB.V4I2.39","url":null,"abstract":"Dengue fever has been endemic to Sri Lanka for several decades. Due to the unavailability of an established prophylactic medicine, dengue prevention depends largely on vector control, where vector surveillance plays a key role. The present study aimed to assess the Aedes mosquito abundance and the risk of disease outbreak using ovitrap surveillance in 14 areas in Sri Lanka, covering four districts with high dengue incidence during 2014 – 2016. A total of 1537 ovitraps were placed in Colombo (Kirulapone, Dematagoda, Grandpass and Thummulla), Gampaha (Kurana and Imbulgoda), Kalutara (Horana, Keselwaththa and Kalamulla) and Kandy (Nawalapitiya, Peradeniya, Edanduwawa, Hanthana Road and Thalwaththa) districts in both indoor and outdoor sites and were collected after five days. The larval counts were used to calculate the Container Index (CI) and Ovitrap Index (OI). Our results revealed significantly higher CI for Aedes species for outdoor compared to indoor sites, indicating a tendency of Ae. aegypti and Ae. albopictus to breed more in outdoor habitats (p 10 % for either or both Aedes species reflecting a possible risk of dengue outbreaks as per the guidelines. Nevertheless, only the abundance of Ae. aegypti (in terms of OI) showed a positive correlation with the number of dengue cases (r = 0.96, p < 0.05) indicating its substantial contribution towards dengue incidences in the studied areas.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131820708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In Vivo effects of zinc oxide-Chromolaena odorata nanoparticle treated Oyun river water 氧化锌纳米颗粒处理奥云河水的体内效应
Sri Lankan Journal of Biology Pub Date : 2019-07-05 DOI: 10.4038/SLJB.V4I2.41
R. Arise, A. B. Alabi, O. Maccarthy, T. M. Olurombi, O. Olufemi, O. S. Farohunbi, T. Anthony
{"title":"In Vivo effects of zinc oxide-Chromolaena odorata nanoparticle treated Oyun river water","authors":"R. Arise, A. B. Alabi, O. Maccarthy, T. M. Olurombi, O. Olufemi, O. S. Farohunbi, T. Anthony","doi":"10.4038/SLJB.V4I2.41","DOIUrl":"https://doi.org/10.4038/SLJB.V4I2.41","url":null,"abstract":"This study evaluated the purifying effect of the zinc oxide-Chromolaena odorata (ZnO-CO) nanoparticle on water samples collected from Oyun River water. Physicochemical and bacteriological characteristics of untreated and treated Oyun river water were determined and their effects on hematological parameters, liver and kidney of rats were also investigated. Thirty-two albino rats (with mean weight ± SD of 132.00 ± 7.50g) were randomized into four groups (A-D). Rats in groups A, B, C and D were maintained on distilled water, untreated Oyun river water (UW), ZnO-CO-treated Oyun river water (ZW), and alum-treated Oyun river water (AW), respectively for 30 days. Thermo-tolerant and enteric bacteria were undetectable in UW samples after treatment with the ZnO-CO nanoparticle. WBC, RBC and platelet counts of rats maintained on UW, ZW, and AW were significantly different from those in the control. Liver aspartate aminotransferase (AST), alanine aminotransferase (ALT) and acid phosphatase (ACP) activities of rats maintained on ZW were increased significantly (p<0.05) when compared to the control. Serum globulin and creatinine concentrations of rats maintained on ZW also increased significantly when compared with the control. The results of this study suggest that Oyun river water is polluted, and its consumption may produce deleterious effects in humans. Treatment of Oyun river water with the synthesized ZnO-CO nanoparticle is more effective than alum in purifying the polluted water. However, ZW also impacted negatively on the liver and renal functions of rats. Hence, ZnO-CO nanoparticle treated water may not be safe as drinking water.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117181829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soil- transmitted nematode infections in school children in a peri-urban area, North Central Nigeria 尼日利亚中北部城郊地区学童土壤传播的线虫感染
Sri Lankan Journal of Biology Pub Date : 2019-07-05 DOI: 10.4038/SLJB.V4I2.38
O. Taiwo, L. E. Edungbola, A. Nyamngee, R. T. Ikpi, E. C. Amaechi
{"title":"Soil- transmitted nematode infections in school children in a peri-urban area, North Central Nigeria","authors":"O. Taiwo, L. E. Edungbola, A. Nyamngee, R. T. Ikpi, E. C. Amaechi","doi":"10.4038/SLJB.V4I2.38","DOIUrl":"https://doi.org/10.4038/SLJB.V4I2.38","url":null,"abstract":"Parasitic infections caused by Soil Transmitted Nematodes (STN) are a common occurrence in developing countries with unfavourable environmental conditions that favour the development of eggs and larvae of STN. This was a cross sectional study carried out from January to April, 2018 to determine the prevalence and intensity of STN infections in six randomly selected primary schools in the Ilorin South Local Government Area, Kwara State, Nigeria. Stool specimens were collected from 508 pupils (259 males; 249 females), which were prepared and examined using the Kato-Katz technique. Of 508 stool specimens examined, 206 (40.6%) were infected with at least one of the STN. Eggs of Ascaris lumbricoides, Trichuris trichiura, Enterobius vermicularis and hookworms were identified with prevalences of 37.9%, 4.9%, 2.4% and 30.1% respectively. In all six schools examined, 115 males (44.4%) and 91 females(36.5%) were found to be infected (P<0.01). Pupils in the age group 5-7 years were found to be the most infected (52.2%), while the least occurred in the age group 14-16 years (28.2%). Of the 206 (40.6%) infected pupils; light, moderate and heavy infections were noted in 12.2%,20.7% and 7.5% respectively. The egg-count of Ascaris lumbricoides, Trichuris trichiura, hookworms and Enterobius vermicularis were 277.8±168.4, 160±118.1, 199.7±131.4 and 33.6±13.1 eggs per gram of faeces respectively. The intensity of STN infections was higher among males (231.3±159.8) than among females (230.8±154.8). Pupils in the age group 11-13 had the highest intensity of infection (267.0±158.1). Prevalence of single and double infections was 75.3% and 24.7% respectively. STN infections are prevalent in the study area which calls for regular deworming, health education and provision of basic sanitary and social amenities to reduce the disease burden.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131456281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Isolation and molecular identification of dominant fungal endophytes from green leaves of physic nut (Jatropha curcas) from unilorin plantation, Ilorin, Nigeria 尼日利亚Ilorin unorin种植园物理坚果(Jatropha curcas)绿叶优势真菌内生菌的分离与分子鉴定
Sri Lankan Journal of Biology Pub Date : 2019-01-30 DOI: 10.4038/SLJB.V4I1.33
A. Lateef, T. Garuba, G. Saad, M. Olesin, G. G. Eperetun, B. B. Tiamiyu
{"title":"Isolation and molecular identification of dominant fungal endophytes from green leaves of physic nut (Jatropha curcas) from unilorin plantation, Ilorin, Nigeria","authors":"A. Lateef, T. Garuba, G. Saad, M. Olesin, G. G. Eperetun, B. B. Tiamiyu","doi":"10.4038/SLJB.V4I1.33","DOIUrl":"https://doi.org/10.4038/SLJB.V4I1.33","url":null,"abstract":"Jatropha curcas is an important crop grown in the plantations of the University of Ilorin for research and commercialization. J. curcas has been reported to have high tolerance for stress, pests and diseases. Different parts of Jatropha are utilised for various uses both domestically and medicinally, thus, this study was carried out to unravel the dominant endophytic fungi associated with green leaves of J. curcas in the said plantation. The dominant fungi were isolated and characterized using the internal transcribed spacer (ITS) region of the ribosomal DNA. Green leaves of J. curcas were collected and processed for isolation of endophytic fungi using water agar. The putative endophytic fungi from J. curcas were identified using morphological and molecular methods as Curvularia geniculata and Phyllosticta capitalensis. The results of this study will be an addition to the fungal endophytes identified on J. curcas and a potential source of secondary metabolite production. This study constitutes, to the authors’ knowledge, the first report of C. geniculata and P. capitalensis as endophytes in J. curcas leaves, in Nigeria.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130219460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Occurrence and prevalence of parasites associated with Gnathonemus senegalensis in Apodu reservoir, Malete, Nigeria 尼日利亚马勒特Apodu水库塞内加尔狭颌鼠相关寄生虫的发生和流行
Sri Lankan Journal of Biology Pub Date : 2019-01-30 DOI: 10.4038/SLJB.V4I1.27
S. O. Oladipo, O. Sunday, D. Ogunbiyi
{"title":"Occurrence and prevalence of parasites associated with Gnathonemus senegalensis in Apodu reservoir, Malete, Nigeria","authors":"S. O. Oladipo, O. Sunday, D. Ogunbiyi","doi":"10.4038/SLJB.V4I1.27","DOIUrl":"https://doi.org/10.4038/SLJB.V4I1.27","url":null,"abstract":"The prevalence of ecto- and endo-parasites of Gnathonemus senegalensis (Trunkfish) was determined with the view of quantifying the helminthic burden in Apodu reservoir, Malete. A total of 150 fish of G. senegalensis were caught with gill nets and cast nets between November 2017 and April, 2018. The fish were transported to the Parasitology laboratory, Kwara state University Malete, for parasite examination. The result shows a prevalence rate of 42% (n=150) with Camallanus sp. and Capillaria sp. having prevalence rates of 11.1 % and 60.32%, respectively. Diphyllobothriumlatum, Acanthocephalan sp., Neascus sp., Tetrahymena sp. and Chilodonella sp. had the prevalence rates of 46.03%, 20.63%, 30.16%, 19.05% and 20.63%, respectively. The ranges of physico-chemical parameters of the water body were: temperature (26.4 -26.9 oC); conductivity (66 – 83 μs/cm); pH (7.54 - 7.98); transparency (110 -402 cm); water current (0.04 -0.42 m/s) and total dissolved solids (34 – 44 ppm). The findings suggest that the observed parasitic infections may adversely affect the economic value of G. senegalensis and if not well managed, could also infect human beings who consume the fish.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116716199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Prevalence of Schistosoma haematobium infection among school-aged children in Afikpo North local government area, Ebonyi State, Nigeria 尼日利亚埃邦伊州Afikpo North地方政府地区学龄儿童血血吸虫感染流行情况
Sri Lankan Journal of Biology Pub Date : 2018-06-30 DOI: 10.4038/SLJB.V3I2.22
P. C. Nwachukwu, C. Ohaeri, O. M. Ukpai, O. P. Irole-Eze, E. C. Amaechi
{"title":"Prevalence of Schistosoma haematobium infection among school-aged children in Afikpo North local government area, Ebonyi State, Nigeria","authors":"P. C. Nwachukwu, C. Ohaeri, O. M. Ukpai, O. P. Irole-Eze, E. C. Amaechi","doi":"10.4038/SLJB.V3I2.22","DOIUrl":"https://doi.org/10.4038/SLJB.V3I2.22","url":null,"abstract":"The present study aimed at evaluating the prevalence of Schistosoma haematobium among school children of ages 5 to 16 years in Afikpo North L.G.A. of Ebonyi State, Nigeria. The study was carried out between February 2016 and January 2017. Urine samples were analyzed in the laboratory using the sedimentation and centrifugation technique. Of 504 persons (250 males and 254 females) that were randomly selected and screened for S. haematobium infection, 20 (3.97 %) were found to be infected. The intensity of infection was greater in males (n=12; 2.38 %) than in the females (n =8; 1.59 %). The sex of an individual was not statistically associated with the prevalence of S. haematobium. The highest prevalence rate of S. haematobium was recorded in the age bracket of 14 -16 yrs. 7 (1.39 %) while the least prevalent was recorded among the 5 – 7 yrs.2 (0.40 %). There is no significant relationship between the age and the prevalence of S. haematobium (p> 0.05). No case of infection was found in the pupils using boreholes and rain water. The highest prevalence with respect to the source of water was in pupils using the stream 15(2.98%). There was a significant relationship between the source of water and the prevalence of S. haematobium. Additionally, there was no significant relationship between occupation of the parents and the prevalence of S. haematobium (p> 0.05). The results reveal that the prevalence of S. haematobium is not location specific, but there is a need to integrate the control of snails to reduce the prevalence of S. haematobium in Afikpo North L.G.A. Ebonyi State.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123389793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Morphometric phenotypes and molecular identification of Fasciola species isolated from cattle in Ilorin, North-Central Nigeria 尼日利亚中北部伊洛林牛片形吸虫分离种的形态计量学表型和分子鉴定
Sri Lankan Journal of Biology Pub Date : 2018-06-30 DOI: 10.4038/SLJB.V3I2.23
O. A. Iyiola, O. Shittu, O. A. Owolodun, D. A. Animasaun, A. Udeze
{"title":"Morphometric phenotypes and molecular identification of Fasciola species isolated from cattle in Ilorin, North-Central Nigeria","authors":"O. A. Iyiola, O. Shittu, O. A. Owolodun, D. A. Animasaun, A. Udeze","doi":"10.4038/SLJB.V3I2.23","DOIUrl":"https://doi.org/10.4038/SLJB.V3I2.23","url":null,"abstract":"Fascioliasis is a serious infectious parasitic disease of human and domestic ruminants. It is caused by the common liver flukes Fasciola hepatica and Fasciola gigantic and it is of public health concern in some countries of the world. Species-level identification of these species using only morphological methods is unconvincing, unreliable and sometimes difficult. Molecular methods enhance the prospects of species-level identifications using a well established and highly reliable DNA-based approach. We used PCR-RFLP analysis for precise identification of Fasciola species collected from Nigeria. One hundred Fasciola adult worms were collected from selected abattoirs in Ilorin, North-central Nigeria. The parasitic worms were first identified by morphometric analysis using AmScope digital camera fastened to the ocular eye piece of the light microscope. For the molecular identifications, genomic DNA was extracted, Polymerase Chain Reaction (PCR) technique and RFLP were used to characterise the Fasciola adult worms. Morphometric data showed that 70 out of the 100 Fasciola adult worms have characteristic features similar with that of F. gigantica as described from previous studies while the remaining 30 adult worms have characteristics that deviate from that of F. gigantica but did not confirm with characteristic features of F. hepatica. Agarose gel electrophoresis result confirmed successful amplification of two gene regions of Sodium Dehydrogenase I (ND1) from mitochondrial DNA of Fasciola spp. The PCR-RFLP analysis did not show any noticeable genetic variation in the Fasciola adult worms. We suggest more studies to be done using DNA sequence analysis and other molecular markers such as random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) to better understand the genetic variability and population structure within Fasciola spp. in Nigeria.","PeriodicalId":145536,"journal":{"name":"Sri Lankan Journal of Biology","volume":"258 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132720139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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