International journal of systematic bacteriology最新文献_第3页

Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei. pealeana Shewanella sp. nov.，一种与鱿鱼附属底腺相关的微生物群落成员。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1341

M R Leonardo, D P Moser, E Barbieri, C A Brantner, B J MacGregor, B J Paster, E Stackebrandt, K H Nealson

{"title":"Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei.","authors":"M R Leonardo, D P Moser, E Barbieri, C A Brantner, B J MacGregor, B J Paster, E Stackebrandt, K H Nealson","doi":"10.1099/00207713-49-4-1341","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1341","url":null,"abstract":"A new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1T (T = type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid Loligo pealei. It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2.0-3.0 microns long, 0.4-0.6 micron wide). ANG-SQ1T grows optimally over the temperature range of 25-30 degrees C and a pH range of 6.5-7.5 degrees C in media containing 0.5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the gamma-3 subclass of the Proteobacteria. The closest relative of ANG-SQ1T is Shewanella gelidimarina, with a 16S rRNA sequence similarity of 97.0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine-N-oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG-SQ1T was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1T is a Shewanella species. Thus it is proposed that this new isolate be assigned to the genus Shewanella and that it should be named Shewanella pealeana sp. nov., in recognition of its association with L. pealei.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1341-51"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 74

Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto. 狭义酵母菌的独特电泳同工酶谱。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1907

F L Duarte, C Pais, I Spencer-Martins, C Leão

{"title":"Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto.","authors":"F L Duarte, C Pais, I Spencer-Martins, C Leão","doi":"10.1099/00207713-49-4-1907","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1907","url":null,"abstract":"Genetic variation among 35 strains representing the four currently recognized species of Saccharomyces sensu stricto (Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces pastorianus/carlsbergensis and Saccharomyces paradoxus) was estimated by analysing the electrophoretic mobilities of nonspecific esterases, acid phosphatase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase isoenzymes. Twenty-two electrophoretic types were identified, a result in agreement with the phenotypic and genetic polymorphisms reported for this group of yeasts. However, the four species were clearly distinguishable based on the patterns obtained using three of the enzymes assayed, the resolving power not being improved by the introduction of data correspondent to lactate dehydrogenase. The overall diversity was higher among S. cerevisiae isolates, in contrast with S. paradoxus which showed only two patterns, one of which was common to four of the five strains studied. Concordant results from the application of the method and DNA hybridization experiments demonstrate its value for identification purposes.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1907-13"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1907","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae. 肠杆菌科沙雷氏菌、布氏菌及其他属的系统发育地位。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1433

C Spröer, U Mendrock, J Swiderski, E Lang, E Stackebrandt

{"title":"The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae.","authors":"C Spröer, U Mendrock, J Swiderski, E Lang, E Stackebrandt","doi":"10.1099/00207713-49-4-1433","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1433","url":null,"abstract":"The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis. Several sequences of strains from the genera Citrobacter, Erwinia, Pantoea, Proteus, Rahnella and Serratia, analysed in this study, have been analysed previously. However, as the sequences of this study differ slightly from the published ones, they were included in the analysis. Of the 23 enterobacterial genera included in an overview dendrogram of relatedness, members of the genera Xenorhabdus, Photorhabdus, Proteus and Plesiomonas were used as a root. The other genera formed two groups which could be separated, although not exclusively, by signature nucleotides at positions 590-649 and 600-638. Group A contains species of Brenneria, Buttiauxella, Citrobacter, Escherichia, Erwinia, Klebsiella, Pantoea, Pectobacterium and Salmonella. All seven type strains of Buttiauxella share 16S rDNA similarities greater than 99%. Group B embraces two phylogenetically separate Serratia clusters, a lineage containing Yersinia species, Rahnella aquatica, Ewingella americana, and also the highly related pair Hafnia alvei and Obesumbacterium proteus.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1433-8"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1433","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21414636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 139

Dolosicoccus paucivorans gen. nov., sp. nov., isolated from human blood. 从人血中分离的少孢球菌。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1439

M D Collins, M Rodriguez Jovita, R A Hutson, E Falsen, B Sjödén, R R Facklam

引用次数: 19

Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov., comb. nov. lentum真杆菌向蛋菌属(Eggerthella lenta gen. nov.， comb.)转移的系统发育证据。11月。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1725

A Kageyama, Y Benno, T Nakase

{"title":"Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov., comb. nov.","authors":"A Kageyama, Y Benno, T Nakase","doi":"10.1099/00207713-49-4-1725","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1725","url":null,"abstract":"Eubacterium lentum has unique phenotypic characters within the genus Eubacterium. The 16S rRNA sequence of Eubacterium lentum was determined and its phylogenetic position was defined. This micro-organism is a member of the genus Eubacterium but it is not closely related to Eubacterium limosum, the type species of the genus Eubacterium, and is nearer to Collinsella aerofaciens and Coriobacterium glomerans. A PCR-based identification system using species-specific primers designed on the basis of DNA sequences encoding the 16S rRNA of strains of Eubacterium lentum, Collinsella aerofaciens and Coriobacterium glomerans is described. A species-specific primer set can distinguish Eubacterium lentum from Eubacterium limosum or closely related species including Collinsella aerofaciens, Coriobacterium glomerans and Atopobium species. This species-specific PCR method can be used to identify Eubacterium lentum-like species isolated from human faeces. On the basis of the 16S rRNA sequence divergence from Collinsella aerofaciens and Coriobacterium glomerans and the presence of unique phenotypic characters, a new genus, Eggerthella gen. nov., is proposed for Eubacterium lentum, with one species, Eggerthella lenta comb. nov. The type strain of Eggerthella lenta is JCM 9979T.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1725-32"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1725","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21414854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 101

Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium. 热厌氧细菌嗜铁菌，一种新型的异化铁(III)还原，厌氧，嗜热细菌。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1471

A I Slobodkin, T P Tourova, B B Kuznetsov, N A Kostrikina, N A Chernyh, E A Bonch-Osmolovskaya

{"title":"Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium.","authors":"A I Slobodkin, T P Tourova, B B Kuznetsov, N A Kostrikina, N A Chernyh, E A Bonch-Osmolovskaya","doi":"10.1099/00207713-49-4-1471","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1471","url":null,"abstract":"A thermophilic, anaerobic, spore-forming, dissimilatory Fe(III)-reducing bacterium, designated strain SR4T, was isolated from sediment of newly formed hydrothermal vents in the area of the eruption of Karymsky volcano on the Kamchatka peninsula. Cells of strain SR4T were straight-to-curved, peritrichous rods, 0.4-0.6 micron in diameter and 3.5-9.0 microns in length, and exhibited a slight tumbling motility. Strain SR4T formed round, refractile, heat-resistant endospores in terminally swollen sporangia. The temperature range for growth was 39-78 degrees C, with an optimum at 69-71 degrees C. The pH range for growth was 4.8-8.2, with an optimum at 6.3-6.5. Strain SR4T grew anaerobically with peptone as carbon source. Amorphous iron(III) oxide present in the medium stimulated the growth of strain SR4T; cell numbers increased with the concomitant accumulation of Fe(II). In the presence of Fe(III), strain SR4T grew on H2/CO2 and utilized molecular hydrogen. Strain SR4T reduced 9,10-anthraquinone-2,6-disulfonic acid, sulfite, thiosulfate, elemental sulfur and MnO2. Strain SR4T did not reduce nitrate or sulfate and was not capable of growth with O2. The fermentation products from glucose were ethanol, lactate, H2 and CO2. The G + C content of DNA was 32 mol%. 16S rDNA sequence analysis placed the organism in the genus Thermoanaerobacter. On the basis of physiological properties and phylogenetic analysis, it is proposed that strain SR4T (= DSM 12299T) should be assigned to a new species, Thermoanaerobacter siderophilus sp. nov.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1471-8"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 118

Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis. 根瘤菌;含羞草，一种从含羞草中分离的新生物变种。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1479

E T Wang, M A Rogel, A García-de los Santos, J Martínez-Romero, M A Cevallos, E Martínez-Romero

{"title":"Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis.","authors":"E T Wang, M A Rogel, A García-de los Santos, J Martínez-Romero, M A Cevallos, E Martínez-Romero","doi":"10.1099/00207713-49-4-1479","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1479","url":null,"abstract":"Fifty rhizobial isolates from root nodules of Mimosa affinis, a small leguminous plant native to Mexico, were identified as Rhizobium etli on the basis of the results of PCR-RFLP and RFLP analyses of small-subunit rRNA genes, multilocus enzyme electrophoresis and DNA-DNA homology. They are, however, a restricted group of lineages with low genetic diversity within the species. The isolates from M. affinis differed-from the R. etli strains that orginated from bean plants (Phaseolus vulgaris) in the size and replicator region of the symbiotic plasmid and in symbiotic-plasmid-borne traits such as nifH gene sequence and organization, melanin production and host specificity. A new biovar, bv. mimosae, is proposed within R. etli to encompass Rhizobium isolates obtained from M. affinis. The strains from common bean plants have been designated previously as R. etli bv. phaseoli. Strains of both R. etli biovars could nodulate P. vulgaris, but only those of bv. mimosae could form nitrogen-fixing nodules on Leucaena leucocephala.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1479-91"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1479","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 108

Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases. 溶卵磷脂密螺旋体，一种小型溶糖螺旋体，其磷脂酶a和C活性与牙周病有关。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1329

C Wyss, B K Choi, P Schüpbach, A Moter, B Guggenheim, U B Göbel

{"title":"Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases.","authors":"C Wyss, B K Choi, P Schüpbach, A Moter, B Guggenheim, U B Göbel","doi":"10.1099/00207713-49-4-1329","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1329","url":null,"abstract":"Strong phospholipase A (PLA) and phospholipase C (PLC) activities as potential virulence factors are the outstanding characteristics of eight strains of small oral spirochaetes isolated from deep periodontal lesions. By qualitative dot-blot DNA-DNA hybridization and 16S rDNA sequence comparison, these spirochaetes form a distinct phylogenetic group, with Treponema maltophilum as its closest cultivable relative. Growth of these treponemes, cells of which contain two endoflagella, one at each pole, was autoinhibited by the PLA-mediated production of lysolecithin unless medium OMIZ-Pat was prepared without lecithin. N-Acetylglucosamine was essential and D-ribose was stimulatory for growth. All isolates were growth-inhibited when 1% foetal calf serum was added to the medium. Growth on agar plates supplemented with human erythrocytes produced haemolysis. In addition to PLA and PLC, the new isolates displayed strong activities of alkaline and acid phosphatases, beta-galactosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase and sialidase, intermediate activities of C4- and C8-esterases, naphthol phosphohydrolase and alpha-fucosidase and a distinctive 30 kDa antigen detectable on Western blots. This phenotypically and genotypically homogeneous group is proposed as a novel species, Treponema lecithinolyticum sp. nov., with isolate OMZ 684T designated as the type strain. A molecular epidemiological analysis using a T. lecithinolyticum-specific probe showed this organism to be associated with affected sites when compared with unaffected sites of periodontitis patients. This association was more pronounced in patients with rapidly progressive periodontitis than in those with adult periodontitis.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1329-39"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Kluyveromyces nonfermentans sp. nov., a new yeast species isolated from the deep sea. 从深海分离的一种新酵母菌(Kluyveromyces nonfermentans sp. nov.)。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1899

T Nagahama, M Hamamoto, T Nakase, K Horikoshi

引用次数: 47

Phylogenetic characterization of 'Candidatus Helicobacter bovis', a new gastric helicobacter in cattle. 牛新胃幽门杆菌“牛候补幽门杆菌”的系统发育特征。

International journal of systematic bacteriology Pub Date : 1999-10-01 DOI: 10.1099/00207713-49-4-1707

D De Groote, L J van Doorn, R Ducatelle, A Verschuuren, K Tilmant, W G Quint, F Haesebrouck, P Vandamme

{"title":"Phylogenetic characterization of 'Candidatus Helicobacter bovis', a new gastric helicobacter in cattle.","authors":"D De Groote, L J van Doorn, R Ducatelle, A Verschuuren, K Tilmant, W G Quint, F Haesebrouck, P Vandamme","doi":"10.1099/00207713-49-4-1707","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1707","url":null,"abstract":"Recently helicobacter-like organisms have been reported in the pyloric part of the abomasum of calves and adult cattle. Cultivation of these spiral bacteria has not been successful to date. In the present study, comparative 16S rDNA sequence analysis was used to determine the taxonomic position of these bacteria. Seven abomasal biopsies of adult cattle were sampled from different Belgian and Dutch farms. In all samples the presence of helicobacter-like organisms was demonstrated by biochemical, immunohistochemical and electron microscopical data. Bacterial 16S rDNA was amplified by PCR and sequences were determined either by direct or indirect sequence analysis. Pairwise comparisons revealed all sequences to be more than 99% homologous. Phylogenetic analysis placed the organism, corresponding to the reference sequence R2XA, within the genus Helicobacter. A diagnostic PCR assay was designed, differentiating all of the bovine 16S rDNA sequences from Helicobacter and Wolinella species. The low similarity level towards Helicobacter bilis (92.8%), its closest validly named neighbour, indicates that this novel taxon is indeed a novel Helicobacter species. An in situ hybridization procedure associated the bovine sequences to the helicobacter-like organisms in the abomasum. The name 'Candidatus Helicobacter bovis' is proposed for this new abomasal helicobacter from cattle.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1707-15"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21414852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49