International Journal of Research in Pharmaceutical Sciences and Technology最新文献

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Development and validation of new analytical method for the simultaneous estimation of amitriptyline and perphenazine in bulk and pharmaceutical dosage form by RP-HPLC 反相高效液相色谱法同时测定原料药和制剂中阿米替林和哌那嗪含量的新方法的建立与验证

International Journal of Research in Pharmaceutical Sciences and Technology Pub Date : 2018-05-23 DOI: 10.33974/ijrpst.v1i1.19

V. P. Kumar, B. Ramadevi, S. Karishma, P. Divya, B. Sivagami, M. Niranjan

{"title":"Development and validation of new analytical method for the simultaneous estimation of amitriptyline and perphenazine in bulk and pharmaceutical dosage form by RP-HPLC","authors":"V. P. Kumar, B. Ramadevi, S. Karishma, P. Divya, B. Sivagami, M. Niranjan","doi":"10.33974/ijrpst.v1i1.19","DOIUrl":"https://doi.org/10.33974/ijrpst.v1i1.19","url":null,"abstract":"A new, simple, precise, accurate and reproducible RP-HPLC method for simultaneous estimation of Amitriptyline and Perphenazine in bulk and pharmaceutical formulations was developed. Separation of Amitriptyline and Perphenazine was successfully achieved on Inertsil ODS (250x4.6mm) 5µm column in an isocratic mode utilizing Methanol: ACN: Water (50:30:20) at a flow rate of 1.0 ml/min and eluents were monitored at 253nm with a retention time of 2.440 and 5.503 minutes for Amitriptyline and Perphenazine respectively. The method was validated and it was found to be linear. The values of the correlation coefficient were found to 0.992 for Amitriptyline and 0.9992 for Perphenazine respectively. The LOD for Perphenazine and Amitriptyline were found to be and 33.8µg/ml and 4.2 µg/ml. The LOQ for Perphenazine and Amitriptyline were found to be 20.88µg/ml and 12.12µg/ml respectively. The percentage recoveries for Amitriptyline and Perphenazine were found to be within the limit indicates that the proposed method is highly accurate. The method was extensively validated according to ICH guidelines.","PeriodicalId":14281,"journal":{"name":"International Journal of Research in Pharmaceutical Sciences and Technology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80827935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical toxicological evaluation of Aloe vera health drinks in wistar rats 芦荟保健饮料对wistar大鼠的临床前毒理学评价

International Journal of Research in Pharmaceutical Sciences and Technology Pub Date : 2017-05-17 DOI: 10.33974/ijrpst.v1i1.33

P. Sudhakar, V. Vinoth Prabhu, B. Jamuna, R.S Adithya, Lakshmi soman, AnuP Joy, R. Anand

{"title":"Preclinical toxicological evaluation of Aloe vera health drinks in wistar rats","authors":"P. Sudhakar, V. Vinoth Prabhu, B. Jamuna, R.S Adithya, Lakshmi soman, AnuP Joy, R. Anand","doi":"10.33974/ijrpst.v1i1.33","DOIUrl":"https://doi.org/10.33974/ijrpst.v1i1.33","url":null,"abstract":"Human consumption of Aloe vera as a beverage has recently increased in popularity. These benefits are controversial with some sources pointing out that the putative effects of aloe are unsupported by clinical studies; it is important that marketed products be tested for toxicities following oral consumption. Hence this study was designed to evaluate the toxicological effect of marketed aloe health drinks. Thirty either sex Wistar rats (200-300gm) were enrolled in this study and are divided into 5 groups. Group I receives Normal saline serves as vehicle control, Group II and III receives Product A- Low dose (0.5 ml twice daily, p.o) and High dose (1.0 ml twice daily, p.o) respectively. Group IV and V receives Product B- Low dose (0.5 ml twice daily, p.o) and High dose (1.0 ml twice daily, p.o) respectively. Weekly body weight and daily feed intake were measured. On 28th day total urine output volume, faecal consistency, Haematological, biochemical, and organ weight were measured to assess the toxicity of aloe health drinks. The result of this study shows that continuous usage of aloe health drinks showed milder weight reduction, significant improvement in erythropoiesis also it increases the WBC count and increases the weight of spleen it may confirm the immune modulatory effect of aloe health drink. At the higher doses, it increased the SGOT, SGPT, serum urea and creatinine it may lead to the hepatotoxicity and nephrotoxicity. In gastrointestinal tract on prolonged uses, it produced few lesions and diarrhoea. It might be concluded that prolonged consumption of unprocessed aloe health drink contains latex, an ingredient which has many health risks associated with it. So it can aggravate health problems.","PeriodicalId":14281,"journal":{"name":"International Journal of Research in Pharmaceutical Sciences and Technology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81642084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Phytochemical, physicochemical, TLC, minerals analysis and in-vitro antioxidant activity of ethanolic extract of leaves of Heldigardia populifolia 白杨叶乙醇提取物的植物化学、理化、薄层色谱、矿物质分析及体外抗氧化活性研究

International Journal of Research in Pharmaceutical Sciences and Technology Pub Date : 2016-09-30 DOI: 10.33974/ijrpst.v1i1.32

P. Keerthana, T. Bhanumathi, A. Cheenakesavulu, M. Neeharika, E. Sandhya, B. Thabitha

引用次数: 0

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