International journal of radiation biology and related studies in physics, chemistry, and medicine最新文献

Repair of potentially lethal damage in unfed plateau phase cultures of Ehrlich ascites tumour cells. I. Suspension cultures. 埃利希腹水肿瘤细胞高原期培养中潜在致命损伤的修复。1 .悬浮培养。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 2009-07-03 DOI: 10.1080/09553008014550751

G. Iliakis

引用次数: 1

Variations in the spectrum of lesions produced in the DNA of cells from mouse tissues after exposure to gamma-rays in air-breathing or in artificially anoxic animals. 暴露于呼吸空气或人工缺氧动物的伽马射线后，小鼠组织细胞DNA中病变谱的变化。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551291

D Murray, R E Meyn, S C Vanankeren

{"title":"Variations in the spectrum of lesions produced in the DNA of cells from mouse tissues after exposure to gamma-rays in air-breathing or in artificially anoxic animals.","authors":"D Murray, R E Meyn, S C Vanankeren","doi":"10.1080/09553008814551291","DOIUrl":"https://doi.org/10.1080/09553008814551291","url":null,"abstract":"Gamma-ray-induced DNA-protein crosslinks (dpc) are preferentially induced in cultured cells irradiated at very low oxygen tensions (Meyn et al. 1987). Since some cells within mouse tumors may be radiobiologically hypoxic, dpc may also be induced in such cells after irradiation in vivo. To examine this possibility, mice bearing either an FSa or NFSa fibrosarcoma in their hind legs were whole-body irradiated either while breathing atmospheric oxygen or 15 min after cervical dislocation, which induces uniform anoxia. DNA single-strand breaks (ssb) and dpc were then assayed both in tumors and normal tissues by alkaline elution. The level of dpc was inferred from the observed increase in ssb yield after digestion of the cell lysates with proteinase K. In addition, cell suspensions were irradiated in vitro, on ice, exposed to atmospheric oxygen tensions. Few dpc were detected in the DNA from tumor cells irradiated in vitro; however, in cells from both FSa and NFSa tumors irradiated in situ there was a significant level of protein-concealed ssb, and thus of dpc. These data are most likely the result of the relative hypoxia of a proportion of cells from both the FSa and NFSa tumor in the air-breathing animals. Induction of dpc was further enhanced in the DNA from tumor cells irradiated under anoxic conditions. A significant level of dpc was also observed in jejunal and spleen cells irradiated in vivo; however, since a significant level of protein-concealed breaks was also observed in cells irradiated in vitro, oxygenation appears not to be the only parameter capable of modifying the proportion of protein-concealed ssb, and the effects of proteinase K on the DNA elution rate for normal mouse tissues may be complex.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"921-33"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effects of ionizing radiation on the metabolism and longitudinal growth of cartilaginous embryonic chick tibiae in vitro. 电离辐射对离体软骨胚鸡胫骨代谢和纵向生长的影响。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551321

L de Ridder, H Thierens, M Cornelissen, O Segaert

{"title":"Effects of ionizing radiation on the metabolism and longitudinal growth of cartilaginous embryonic chick tibiae in vitro.","authors":"L de Ridder, H Thierens, M Cornelissen, O Segaert","doi":"10.1080/09553008814551321","DOIUrl":"https://doi.org/10.1080/09553008814551321","url":null,"abstract":"The effect of ionizing radiation on the metabolism and longitudinal growth of cartilaginous tibiae of 6.5-day-old chick embryos was studied in vitro over a 3-day period. Before being cultured, tibiae received absorbed doses of 2 to 200 Gy. Of each pair, the counterpart served as control. Compared to the strong inhibition of [3H]thymidine incorporation, already 50 percent at 10 Gy, the effects of ionizing radiation on [3H]uridine and [3H]proline incorporation were limited: 20 and 40 percent respectively at 150 Gy. Metabolism of the cartilage cells in our organ culture was almost completely arrested at 200 Gy. Light and electron microscopy showed no morphological differences between irradiated and sham-irradiated tibiae until 150 Gy. At 200 Gy necrosis of most of the cells was observed. No differences in form and arrangement of extracellular fibers were noticed. The results of the metabolic studies and the morphological observations were correlated with the effects of ionizing radiation on the longitudinal growth. In contrast to DNA synthesis, RNA transcription and synthesis of collagen fibres were radioresistant processes.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"965-75"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551321","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13601830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Stochastic model of free radical yields in oriented DNA exposed to densely ionizing radiation at 77K. 取向DNA在77K强电离辐射下自由基产率的随机模型。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551261

J H Miller, W E Wilson, C E Swenberg, L S Myers, D E Charlton

引用次数: 10

Effect of non-volatile scavengers of hydroxyl radicals on thymine radical formation induced by gamma-rays and ultrasound. 非挥发性羟基自由基清除剂对γ射线和超声诱导胸腺嘧啶自由基形成的影响。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551251

T Kondo, C M Krishna, P Riesz

{"title":"Effect of non-volatile scavengers of hydroxyl radicals on thymine radical formation induced by gamma-rays and ultrasound.","authors":"T Kondo, C M Krishna, P Riesz","doi":"10.1080/09553008814551251","DOIUrl":"https://doi.org/10.1080/09553008814551251","url":null,"abstract":"In order to investigate the mechanism of sonolysis of nucleic acid constituents, the yield of thymine radicals generated by 50 kHz ultrasound in Ar-saturated aqueous solution was compared with that formed by gamma-radiolysis in N2O-saturated solutions in the presence of various non-volatile scavengers, which cannot act in the gas phase of the cavitation bubbles. For comparison of thymine radical yields by sonolysis and gamma radiolysis, the method of spin trapping with 3,5-dibromo-4-nitrosobenzenesulphonate (a water-soluble, non-volatile, aromatic nitroso spin trap) combined with ESR was used. The efficiency of OH radical scavenging is expressed by the reciprocal value of C1/2, the scavenger concentration at which the thymine radical yield is decreased by 50 per cent. In gamma radiolysis the scavenging efficiencies of the solutes depend on their rate constants with OH radicals. For sonolysis the C1/2 values were similar to those obtained for gamma radiolysis except for the hydrophobic 5,5-dimethyl-1-pyrroline-N-oxide. These results suggest that thymine radicals induced by ultrasound are produced in the bulk of the solution as well as in the interfacial region.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"891-9"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13973108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Studies of the induction of dominant lethals and translocations in male mice after chronic exposure to microwave radiation. 长期暴露于微波辐射后雄性小鼠显性致死和易位的诱导研究。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551341

R D Saunders, C I Kowalczuk, C V Beechey, R Dunford

{"title":"Studies of the induction of dominant lethals and translocations in male mice after chronic exposure to microwave radiation.","authors":"R D Saunders, C I Kowalczuk, C V Beechey, R Dunford","doi":"10.1080/09553008814551341","DOIUrl":"https://doi.org/10.1080/09553008814551341","url":null,"abstract":"Male C3H mice were exposed to 100 W m-2 of 2.45 GHz continuous-wave microwave radiation for 6 h per day for a total of 120 h over an 8-week period. The exposure level was chosen so that the specific energy absorption rate (SAR) would be approximately equal to the level of 4 W kg-1 which is considered by a number of organizations to be a threshold for adverse biological effects. At the end of the treatment period the mice were mated with a different group of (C3H x 101) F1 hybrid females each week for the following 8 weeks. There was no significant reduction in pregnancy rate, preimplantation survival or postimplantation survival in the exposed group compared to sham-exposed controls. At the end of the mating period a cytogenetic analysis was carried out of meiotic chromosome preparations of testicular tissue, thus sampling cells that were stem cell spermatogonia during the treatment regime. The results showed no difference in the frequency of reciprocal translocations between the sham and treated groups, or in the frequency of cells with autosome or sex chromosome univalents. Low levels of fragments and exchanges were found in both groups. It is concluded that there is no evidence in this experiment to show that chronic exposure of male mice to 2.45 GHz microwave radiation induces a mutagenic response in male germ cells. This conclusion is in agreement with the observations of Berman et al. (1980), who reported a lack of male germ cell mutagenesis after repetitive or chronic exposure of rats to 2.45 GHz.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"983-92"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Modulation of radiation-induced chromosome aberrations by DMSO, an OH radical scavenger. 1: Dose-response studies in human lymphocytes exposed to 220 kV X-rays. OH自由基清除剂DMSO对辐射诱导的染色体畸变的调节。1:人类淋巴细胞暴露于220千伏x射线的剂量反应研究。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551241

L G Littlefield, E E Joiner, S P Colyer, A M Sayer, E L Frome

{"title":"Modulation of radiation-induced chromosome aberrations by DMSO, an OH radical scavenger. 1: Dose-response studies in human lymphocytes exposed to 220 kV X-rays.","authors":"L G Littlefield, E E Joiner, S P Colyer, A M Sayer, E L Frome","doi":"10.1080/09553008814551241","DOIUrl":"https://doi.org/10.1080/09553008814551241","url":null,"abstract":"Human G0 lymphocytes were exposed to 220 kV X-radiation in the presence or absence of DMSO, an efficient selective scavenger of OH radicals. Our studies demonstrate that DMSO affects a concentration-dependent modulation of induced asymmetrical aberrations in human lymphocytes exposed to approximately 3.0 Gy, with maximum protectible fractions of approximately 70 percent at DMSO concentrations of greater than or equal to 1 M. The dose dependency for dicentrics in lymphocytes acutely exposed to X-ray doses of 0.51 to 4.98 Gy in the absence of DMSO is adequately described by the linear-quadratic dose-response function Y = alpha D + beta D2. Data from duplicate cultures exposed in the presence of 1 M DMSO produce an excellent fit to the regression function modified as follows: Y(+ DMSO) = alpha(delta D) + beta(delta D)2 where the 'dose modifying' factor delta = 0.501. We interpret these findings as providing evidence that OH radical-mediated lesions in DNA account for approximately 50 percent of the dose dependency for dicentrics resulting from either one-track or two-track events, following exposures of non-cycling cells to moderate-to-high doses of low LET radiation. These data may be used in additional calculations to derive an estimate of approximately 6 x 10(8) s-1 for the rate of reaction of OH radicals with DNA targets involved in aberration formation.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"875-90"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14388246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

In vivo adenylate cyclase activity in ultraviolet- and gamma-irradiated Escherichia coli. 紫外和γ辐照大肠杆菌体内腺苷酸环化酶活性。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551331

A Chatterjee, A K Bhattacharya

引用次数: 2

Quantitative, functional and biochemical alterations in the peritoneal cells of mice exposed to whole-body gamma-irradiation. I. Changes in cellular protein, adherence properties and enzymatic activities associated with platelet-activating factor formation and inactivation, and arachidonate metabolism. 暴露于全身γ辐射的小鼠腹膜细胞的定量、功能和生化改变。1 .与血小板活化因子形成和失活以及花生四烯酸酯代谢相关的细胞蛋白、粘附特性和酶活性的变化。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551311

L K Steel, H N Hughes, T L Walden

{"title":"Quantitative, functional and biochemical alterations in the peritoneal cells of mice exposed to whole-body gamma-irradiation. I. Changes in cellular protein, adherence properties and enzymatic activities associated with platelet-activating factor formation and inactivation, and arachidonate metabolism.","authors":"L K Steel, H N Hughes, T L Walden","doi":"10.1080/09553008814551311","DOIUrl":"https://doi.org/10.1080/09553008814551311","url":null,"abstract":"Changes in total number, differentials, cell protein, adherence properties, acetyltransferase and acetylhydrolase activities, prostaglandin E2 and leukotriene C4 production, as well as Ca2+ ionophore A23187 stimulation were examined in resident peritoneal cells isolated from mice 2 h to 10 days postexposure to a single dose (7, 10 or 12 Gy) of gamma-radiation. Radiation dose-related reductions in macrophage and lymphocyte numbers and increases in cellular protein and capacity to adhere to plastic surfaces were evident. In vivo irradiation also elevated the activities of acetyltransferase and acetylhydrolase (catalysing platelet-activating factor biosynthesis and inactivation, respectively) in adherent and nonadherent peritoneal cells, particularly 3-4 days postexposure. Blood plasma from irradiated animals did not reflect the increased cellular acetylhydrolase activity. Prostaglandin E2 and leukotriene C4 synthesis were elevated postexposure, suggesting increased substrate (arachidonate) availability and increased cyclooxygenase and lipoxygenase activities. Ionophore stimulation of enzyme activities and eicosanoid release also differed in irradiated peritoneal cells. While the properties of adherence, platelet-activating factor synthesis/inactivation-associated enzyme activities, and eicosanoid production are generally characterized as those of macrophages, lymphocytes or their products may influence or contribute to the observed radiation-induced changes.","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 6","pages":"943-64"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814551311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14414417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Induction of repair functions by hydrogen peroxide in Chinese hamster cells. 过氧化氢对中国仓鼠细胞修复功能的诱导。

International journal of radiation biology and related studies in physics, chemistry, and medicine Pub Date : 1988-06-01 DOI: 10.1080/09553008814551301

S Sen Gupta, S B Bhattacharjee

引用次数: 43