International Journal of Peptide Research and Therapeutics最新文献

{"title":"A Robust Strategy Against Multi-Resistant Pathogens in Oral Health: Harnessing the Potency of Antimicrobial Peptides in Nanofiber-Mediated Therapies","authors":"Karthikeyan Kandaswamy, Raghunandhakumar Subramanian, Jayant Giri, Ajay Guru, Jesu Arockiaraj","doi":"10.1007/s10989-024-10613-x","DOIUrl":"https://doi.org/10.1007/s10989-024-10613-x","url":null,"abstract":"<p>A robust strategy is imperative to counteract multi-resistant pathogens within the oral cavity and mitigate the occurrence of dental-related diseases. According to the recent Global Oral Health Status Report (2022), over 3.5 billion individuals worldwide have been affected, with more than 2 billion experiencing permanent tooth caries. Despite employing various techniques to combat oral pathogens, these microbes persistently proliferate, culminating in biofilm formation that exhibits resistance to conventional antibiotics. Moreover, there is a notable surge in the rate of implant failure attributable to peri-implantitis. Peptide therapy emerges as a promising and enduring solution, demonstrating adaptability to diverse techniques. The pivotal technique involving Antimicrobial peptides (AMPs) orchestrates damage to the extracellular matrix, encompassing the glucan matrix, disrupting microbial content, and inducing pathogen demise. Notably, AMPs actively participate in the intricate process of oral tissue healing. Nanofibers, characterized by their multifaceted properties and robust mechanical capacity, facilitate protracted drug delivery and manifest optimal biocompatibility. This inherent attribute stimulates the application of AMPs, thereby augmenting healing durations and ensuring sustained therapeutic efficacy against oral diseases. The primary focus of this review centers on Antimicrobial peptides (AMPs) integrated into Nanofibers, offering a comprehensive clinical solution to diverse dental-mediated diseases. The exploration of various AMPs and their modes of action, coupled with the efficacy of peptide durability, sets the stage for futuristic enhancements in oral health therapeutics.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"28 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140883459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish Protein Hydrolysate Research Trends over the Last 5 Years and Future Research Predictions; a Bibliometric Analysis","authors":"Islamuddin Jafar, Muhammad Asfar, Meta Mahendradatta, Aidil Zulhaq Paradiman, Muhammad Iqbal","doi":"10.1007/s10989-024-10616-8","DOIUrl":"https://doi.org/10.1007/s10989-024-10616-8","url":null,"abstract":"<p>This bibliometric analysis delves into the latest research on protein hydrolysates from fish, examining its potential to contain various bioactive peptides and other functional compounds that benefit overall health. The study also explores publication trends of fish protein hydrolysates from 2019 to 2023, revealing a total of 564 related articles with an annual growth rate of 6%. Notably, countries such as China, Spain, India, and Norway lead the way in contributing to the field of fish protein hydrolyzate. The top journal in this area is Marine Drugs published by the Multidisciplinary Digital Publishing Institute (MDPI). The most frequently cited studies focus on the development and application of food ingredients, bioactive properties, enzyme use, food allergy prevention, and peptide characterization in relation to fishery products and protein hydrolysates. Keyword analysis highlights functional properties, bioactive peptides, biological activity, and cell proliferation as key areas of interest. Overall, these findings suggest that functional properties, bioactive peptides, and cell proliferation remain promising topic for future research and development.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"46 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Peptides (AMPs): New Perspectives on Their Function in Dermatological Diseases","authors":"Chou-Yi Hsu, Aseel Muthana Yousif, Khairia Abdulrahman Abullah, Huda Hayder Abbas, Hijaz Ahmad, Gaber E. Eldesoky, Mohaned Adil, Zeanb Hussein","doi":"10.1007/s10989-024-10609-7","DOIUrl":"https://doi.org/10.1007/s10989-024-10609-7","url":null,"abstract":"<p>Antimicrobial peptides (AMPs) are small cationic or amphipathic molecules that are produced by both prokaryotic and eukaryotic species. The latest findings in the field of dermatology point to the potential significance of AMPs in the battle against skin microbial infections. AMPs additionally function as multifunctional immune effectors, promoting angiogenesis, wound healing, and the production of cytokines and chemokines. In human skin, AMPs such as β-defensin, S100, and cathelicidin are primarily secreted by keratinocytes, neutrophils, sebocytes, or sweat glands. These substances are either produced continuously or expressed in reaction to certain inflammatory stimuli, thus playing a role in the development of various skin diseases in humans. Furthermore, in contrast to other human skin conditions, the level of AMP synthesis decreases as the disease progresses. In this review, we provide data supporting the role of AMPs as natural mediators of dermatological problems, as well as their potential for being used as therapeutic agents in the treatment of skin diseases.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Expression of Recombinant cell Penetrating Protein Based on Tat and pertussis-like Toxin A and Evaluation of its Effects on the lung cancer","authors":"Arman Mahdavi, Hamideh Mahmoodzadeh Hosseini, Reza Ranjbar, Jafar Amani, Soghra Khani, Seyed Ali Mirhosseini","doi":"10.1007/s10989-024-10611-z","DOIUrl":"https://doi.org/10.1007/s10989-024-10611-z","url":null,"abstract":"<p>Nowadays, design of cytotoxic agents based on microbial toxins is attracted for researchers. Pertussis-like toxin subunit A (PltA) of typhoid toxin is ADP-ribosyl transferase and had the cytotoxicity and cell arrest property in G2/M phase of human cancer cell line. To translocate and its increase to the cells, PltA requires the cell penetrating part. Here, the catalytic PltA (named Typh) was attached to Tat peptide as a cell penetrating agent and expressed as a new recombinant fusion protein (named Tat-Typh) in <i>E. coli</i> BL21. After that, recombinant Tat-Typh was purified using Ni + chromatography column and confirmed by western blotting. Finally, its cytotoxicity effects and cell penetration activity were evaluated by, MTT assay, Annexin-V/PI staining and western blotting methods, respectively. Our results showed that Tat-Typh had the significant cytotoxic effect at 25, 50, 150 and 200 µg/mL concentrations (<i>P</i> &lt; 0.05). In addition, cell treating with 50 µg/mL Tat-Typh was resulted in to increase the percentage of necrotic cells compared to control groups (<i>P</i> &lt; 0.05). Moreover, binding of Tat fragment to Typh protein caused to increase the speed of entry of Tat-Typh to cells compared to Typh alone. In conclusion, it is observed that Tat-Typh protein is able to increase the cell penetration properties of catalytic Pertussis-like toxin subunit A and has the cytotoxic effects on lung cancer cell line.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"36 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140806501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Situ Assembly of Melittin-PHA Microspheres for Enhancing Therapeutic Efficacy in Cancer Treatment","authors":"Xueyu Fan, Chao Zhang, Shuangqing Fu, Shuo Wang, Shuo Ma, Jie Du, Wei Li, Honglei Zhang","doi":"10.1007/s10989-024-10600-2","DOIUrl":"https://doi.org/10.1007/s10989-024-10600-2","url":null,"abstract":"<p>Amphiphilic cationic peptide (ACP) is a widely studied biofilm-active peptide that has great potential in cancer treatment. However, poor stability, a short half-life, and complex preparation pose significant challenges for practical therapeutic applications. In the current investigation, the amphiphilic peptide Melittin (Mel), recognized for its powerful anticancer properties, was chosen from natural and synthetic ACP, and integrated into a nanostructure by utilizing polyhydroxyalkanoate (PHA) microspheres as carriers to produce Mel-loaded PHA microspheres (Mel@PHA-PhaC). Mel@PHA-PhaC nanostructure was self-assembled in <i>Escherichia coli</i>, simplifying its preparation and making it more convenient and high-yield. Mel@PHA-PhaC were spherical, with a particle size of approximately 300 nm, as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The concentration of Mel in Mel@PHA-PhaC was 4 μg/mg. Mel@PHA-PhaC still maintained good stability after being treated with pancreatic enzymes. Furthermore, in vitro experiments demonstrated that Mel@PHA-PhaC enhanced the inhibitory effect on cancer cells compared to free Mel. This study provides insights and guidelines for the development and utilization of peptide delivery systems using PHA microspheres to create stable and improved peptides for cancer therapy.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"63 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-toxicity of Plant Candicidal Peptides for Mammalian Cell Lines and Galleria mellonella Model to Improving Selectivity for Clinical Use","authors":"Milena Bellei Cherene, Marco Calvinho Cavaco, Vera Luisa Santos Neves, Miguel Augusto Rico Botas Castanho, Gabriel Bonan Taveira, Thomas Zacarone Afonso Guimarães, André de Oliveira Carvalho, Erica de Oliveira Mello, Layrana de Azevedo dos Santos, Valdirene Moreira Gomes","doi":"10.1007/s10989-024-10607-9","DOIUrl":"https://doi.org/10.1007/s10989-024-10607-9","url":null,"abstract":"<p>Antimicrobial peptides (AMPs) are promising candidates for the development of new drugs. However, thorough studies on the toxicity of these molecules are scarce, which is a gap, as host toxicity is one of the main reasons for nonapproval of the drug by regulatory agencies. This work aimed to evaluate the toxicity of three AMPs isolated from <i>Capsicum annuum</i> leaves, named <i>Ca</i>CPin-II, <i>Ca</i>CDef-like and <i>Ca</i>CLTP2. The AMP toxicological profile was evaluated by in vitro cytotoxicity against mammalian cells and systemic in vivo toxicity using <i>Galleria mellonella</i> larvae as study model. AMP cytotoxicity was evaluated in a broad panel of human cell lines, namely, vascular endothelium, cervical adenocarcinoma, prostatic epithelium, mammary epithelium and fibroblasts, and in murine macrophages. Cell viability was evaluated through metabolic activity, a gold standard method for assessing viability due to the speed, robustness and reliability of the results. To elucidate the toxicity mechanism of the peptides, their ability to bind to the cell surface and to permeabilize membranes was evaluated by measuring the zeta potential and the absorption of the SYTOX® Green fluorescent probe, respectively. The AMPs did not decrease cell viability or permeabilize the membranes of the cell lines at the tested concentrations. Only <i>Ca</i>CLTP2 had the ability to interact with the cell surface, but it was not able to permeabilize them. The in vivo systemic toxicity was evaluated by the survival rate of the <i>G. mellonella</i> larvae inoculated with peptides. <i>Ca</i>CPin-II showed in vivo toxicity, as the larval survival rate after the test was 60% lower than that of the controls. The results suggest that these peptides have potential as antimicrobial agents because they have low or no toxicity to mammalian cells and can serve as a framework for drug development.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"2022 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Biofilm Effects of Melittin: Lessons Learned and the Path Ahead","authors":"Mojtaba Memariani, Hamed Memariani","doi":"10.1007/s10989-024-10606-w","DOIUrl":"https://doi.org/10.1007/s10989-024-10606-w","url":null,"abstract":"<p>Biofilm formation empowers microorganisms to withstand clearance mechanisms produced by host and synthetic sources. Biofilms are frequently held responsible for recurrent and chronic infectious diseases. Therefore, the development of effective anti-biofilm agents is of great importance. Melittin, the principal component in the venom of European honeybee, has sparked immense interest due to its anti-microbial, anti-cancer, anti-inflammatory, anti-diabetic, anti-neuropathic, wound-healing, and adjuvants properties. Considering the recent growth of research on the anti-biofilm effects of melittin, coupled with the absence of a dedicated review on this subject, the present review summarizes the key findings of the studies conducted thus far. Furthermore, this review offers several potentially fruitful areas for future research. Available evidence suggests that melittin can inhibit biofilm formation by important microbial pathogens such as <i>Acinetobacter baumannii</i>, <i>Borrelia burgdorferi</i>, <i>Enterococcus faecalis</i>, <i>Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus</i>, <i>Streptococcus mutans</i>, and <i>Candida albicans.</i> The multifaceted mechanisms of melittin in combating biofilms are truly impressive, as it prevents microbial adhesion, inhibits biofilm development, downregulates genes crucial for biofilm formation and quorum-sensing pathways, disrupts the biofilm matrix, and eradicates biofilm-entrenched cells. Future investigations should prioritize the utilization of combination therapy with melittin and antibiotics, the implementation of advanced drug delivery systems, chemical modifications, and the conduction of in vivo studies using animal models.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"30 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Urotensin-II Prevents Cartilage Degeneration in a Monosodium Iodoacetate-Induced Rat Model of Osteoarthritis","authors":"Menderes Yusuf Terzi, Hamza Malik Okuyan, İhsan Karaboğa, Cemil Emre Gökdemir, Duygu Tap, Aydıner Kalacı","doi":"10.1007/s10989-024-10605-x","DOIUrl":"https://doi.org/10.1007/s10989-024-10605-x","url":null,"abstract":"","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"17 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JZTX-V, a Sodium Channel Inhibitor, Exhibits Excellent Analgesic Effects in Mouse Models","authors":"Chen Bo, Sun Zhenghua, Zeng Xiongzhi","doi":"10.1007/s10989-024-10603-z","DOIUrl":"https://doi.org/10.1007/s10989-024-10603-z","url":null,"abstract":"<p>JZTX-V, an inhibitor of voltage-gated sodium and potassium channels, is derived from the venom of the spider <i>Chilobrachys jingzhao</i> in China. JZTX-V was synthesized using a solid-phase chemical approach with Fmoc-protected amino acids to explore its function further. The synthetic peptides were purified using reverse-phase high-performance liquid chromatography (RP-HPLC) and then subjected to oxidative refolding under optimal conditions. A unique peak was observed in the RP-HPLC chromatogram for refolded JZTX-V, and the ratio to native JZTX-V was 1:1 for the mixed samples. Subsequently, the analgesic potential of the synthetic peptide was evaluated in mouse models of pain. In the Formarin model, JZTX-V significantly reduced pain scores in 60 min and its efficacy was comparable to that of morphine. JZTX-V also exhibited excellent analgesic effects in models of postoperative pain and mechanical allodynia. However, JZTX-V had no effect on thermal stimulation injury in the hot plate experiment and did not affect motor coordination. These results indicate that JZTX-V effectively alleviates inflammatory pain in animals and provides a promising template for the design of future clinical analgesic drugs.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"25 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naturally Occurring Imidazole Peptides, Carnosine and Anserine Inhibit the Degranulation of Mast Cells and Basophils by Modulating Intracellular Signaling","authors":"K. Yamaki, N. Kamiki, N. Nakatsuka, H. Yonezawa, A. Suzuki, S. Kubo, K. Ito, H. Fujisawa, Y. Koyama, K. Ohta, M. Ohta","doi":"10.1007/s10989-024-10604-y","DOIUrl":"https://doi.org/10.1007/s10989-024-10604-y","url":null,"abstract":"<p>Imidazole peptides possess multiple functions, including antioxidant effects, although their biological activities are largely unclear. Their production in humans and animals suggests their physiological roles and validates their safety for pharmaceutical or supplemental use. This study investigated the in vitro anti-anaphylactic potential of two histidine-containing dipeptides, carnosine and anserine in mast cells and basophils. Carnosine and anserine reduced mast cell degranulation elicited by anti-ovalbumin monoclonal IgE and ovalbumin or ionomycin in rat basophilic leukemia RBL2H3 cells without affecting cell viability. In contrast, interleukin-4 production following stimulation was enhanced in the presence of carnosine. Carnosine and anserine strongly inhibited Akt phosphorylation and moderately inhibited ERK phosphorylation. However, these peptides enhanced the increase in phosphorylated JNK levels upon IgE stimulation. The phosphorylation levels of p38 were not affected by carnosine or anserine. To determine the effect of carnosine on basophils, we established a method for detecting IgE-dependent activation in primary cultured mouse splenic basophils via CD63 expression for the first time. Carnosine treatment significantly reduced the increase in CD63 surface expression, a marker of degranulation, in mouse splenic basophils stimulated with anti-IgE. Flow cytometory analysis revealed that mean fluorescence intensities for non-stimulated, anti-IgE-stimulated, and carnosine-pre-treated/anti-IgE-stimulated basophils were 3853 ± 320, 5548 ± 282, and 3853 ± 203, respectively. The findings indicate carnosine and anserine suppress mast cell and basophil IgE-dependent degranulation. The proposed mechanism of the inhibitory effect is the suppression of the activation of phosphoinositide 3-kinase–Akt. Carnosine and anserine may exert anti-anaphylactic effects under physiological and pathological conditions and serve as safe potential drug candidates.</p>","PeriodicalId":14217,"journal":{"name":"International Journal of Peptide Research and Therapeutics","volume":"48 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}