International journal of peptide and protein research最新文献_第5页

N-->S phosphoryl migration in phosphoryl glutathion. 磷酰谷胱甘肽中N- >S磷酰迁移。

International journal of peptide and protein research Pub Date : 1993-07-01

H J Yang, J Liu, Y F Zhao

引用次数: 0

Affinity purification of a difficult-sequence protein. Implications for the inclusion of capping in synthetic protocols. 难序列蛋白的亲和纯化。将封顶纳入合成方案的影响。

International journal of peptide and protein research Pub Date : 1993-07-01

E Bianchi, M Sollazzo, A Tramontano, A Pessi

引用次数: 0

Recombinant human brain-derived neurotrophic factor (rHuBDNF). Disulfide structure and characterization of BDNF expressed in CHO cells. 重组人脑源性神经营养因子(rHuBDNF)。CHO细胞中BDNF表达的二硫结构及表征。

International journal of peptide and protein research Pub Date : 1993-06-01

C Acklin, K Stoney, R A Rosenfeld, J A Miller, M F Rohde, M Haniu

引用次数: 0

The beta-bend ribbon spiral. Synthesis and conformational analysis in solution and in the crystal state of depsipeptides containing alpha-hydroxyisobutyric acid. 弯曲带螺旋。含α -羟基异丁酸的沉淀肽的合成及其在溶液和晶体状态下的构象分析。

International journal of peptide and protein research Pub Date : 1993-06-01

M Crisma, G Valle, G M Bonora, C Toniolo, G Cavicchioni

{"title":"The beta-bend ribbon spiral. Synthesis and conformational analysis in solution and in the crystal state of depsipeptides containing alpha-hydroxyisobutyric acid.","authors":"M Crisma, G Valle, G M Bonora, C Toniolo, G Cavicchioni","doi":"","DOIUrl":"","url":null,"abstract":"The synthesis and conformational analysis in solution (by FTIR absorption and 1H NMR) and in the crystal state (by X-ray diffraction) of three Hib-containing depsipeptides have been performed. In the crystal state Z-Aib-Hib-Aib-OMe is folded into a type-III beta-bend, while the conformation adopted by Z-Aib-Hib)2-Aib-OMe is a beta-bend ribbon spiral, characterized by two type-III beta-bends with Aib(1)-Hib(2) and Aib(3)-Hib(4) as corner residues, respectively. Both independent molecules in the asymmetric unit of t-Boc-L-Ala-Hib-L-Ala-OMe crystals are folded into a type-II beta-bend. For the Aib-Hib depsipeptides the conformation adopted in the crystal state is also that largely prevailing in solution, whereas for t-Boc-L-Ala-Hib-L-Ala-OMe the beta-bend conformation is significantly less populated in solution. A comparison is also made with: (i) the published crystal-state conformations of fully protected -(Aib)3-, -(Aib)5-, and -L-Ala-Aib-L-Ala- sequences and the beta-bend ribbon spiral generated by (Aib-L-Pro)n oligomers, and (ii) with the herewith described solution preferred conformation of Z-L-Ala-Aib-L-Ala-OMe. The possible use of Hib as an isosteric replacement for Aib in the design of conformationally constrained depsipeptides is briefly discussed.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"41 6","pages":"553-60"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19336118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of helix ends. Amino acid preferences, hydrogen bonding and electrostatic interactions. 螺旋端部设计。氨基酸偏好，氢键和静电相互作用。

International journal of peptide and protein research Pub Date : 1993-05-01

S Dasgupta, J A Bell

{"title":"Design of helix ends. Amino acid preferences, hydrogen bonding and electrostatic interactions.","authors":"S Dasgupta, J A Bell","doi":"","DOIUrl":"","url":null,"abstract":"The amino acid sequence and chemical interactions at the ends of 163 helices were surveyed so as better to understand amino acid preferences previously observed [Richardson, J.S. & Richardson, D.C. (1988) Science 240, 1648-1652]. Amino acid preferences differed from the previous survey in some significant details and in ways that might affect the choice of amino acids during the design of a protein helix. The following major conclusions about helix ends were deduced from additional patterns of amino acid occurrence and interactions that were observed. (1) A specific pair of hydrogen bonds is often observed between a glutamic acid (or glutamine) side chain at the N3 position and the N-cap amide hydrogen, and between the N-cap side chain (often threonine) and the N3 amide hydrogen. This reciprocal interaction may be an important means of stabilizing the N-terminal end of a helix. (2) Negatively charged amino acids (aspartic acid and glutamic acid) at the N-terminal end of helices may be more important in stabilizing protein helices than positively charged residues (chiefly lysine) at the C-terminal end. (3) The identity of the residue at the N-cap position is correlated with the backbone conformation at that position. (4) Aspartic acid (or asparagine) at the N2 or N3 position may adopt a conformation that suggests a hydrogen-bonding interaction with the end of the helix, especially when the N-cap side chain does not form a hydrogen bond with the end of the helix.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"41 5","pages":"499-511"},"PeriodicalIF":0.0,"publicationDate":"1993-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19306384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conformation of dehydropeptides. Synthesis and solution structure of model peptides containing (E) and (Z) dehydroaminobutyric acid residues. 脱氢肽的构象。含有(E)和(Z)脱氢氨基丁酸残基的模型肽的合成和溶液结构。

International journal of peptide and protein research Pub Date : 1993-05-01

A Gupta, V S Chauhan

{"title":"Conformation of dehydropeptides. Synthesis and solution structure of model peptides containing (E) and (Z) dehydroaminobutyric acid residues.","authors":"A Gupta, V S Chauhan","doi":"","DOIUrl":"","url":null,"abstract":"Six model dipeptide methylamides containing dehydroaminobutyric acid (delta Abu) of the type Boc-X-delta z Abu-NHCH3 and Box-X-delta E Abu-NHCH3, X = Ala, Val, Phe (Boc = tert-butoxycarbonyl), have been synthesized and their solution conformations explored using 300 MHz 1H NMR and IR spectroscopy. Studies based on delineation of intramolecularly hydrogen bonded NH groups in CDCl3 and (CD3)2SO revealed that none of the NH groups is appreciably solvent shielded. Difference NOE (Nuclear Overhauser Effect) studies have also failed to detect the presence of any discernible turn structure in these peptides. These studies indicate that the conformational preferences of peptides containing, alpha, beta-dehydroaminobutyric acid are different from those of delta ZPhe and delta ZLeu. It appears that steric interactions due to the beta-substituent in the dehydroamino acid moiety play an important role. Unlike delta ZPhe and delta ZLeu, which have relatively large beta-substituents, phenyl and isopropyl, respectively, and stabilize a beta-turn, the beta-methyl group of delta ZAbu or delta EAbu is readily accommodated in extended conformation. Clearly, the size of beta-substituent in dehydroamino acid crucially influences the conformational preferences. Thus, it may be possible to use different dehydroamino acids to introduce variable but definite constraints in synthetic peptides.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"41 5","pages":"421-6"},"PeriodicalIF":0.0,"publicationDate":"1993-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19307768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-1 beta converting enzyme. Synthesis of hydroxyethyl dipeptide surrogate-containing compounds as potential ICE inhibitors. 白细胞介素-1转化酶。含羟乙基二肽替代物作为潜在ICE抑制剂的合成。

International journal of peptide and protein research Pub Date : 1993-05-01

L A Reiter, J J Martin

引用次数: 0

Association of beta-agonists with corresponding beta 2- and beta 1-adrenergic pentapeptide sequences. 受体激动剂与相应的β 2-和β 1-肾上腺素能五肽序列的关联。

International journal of peptide and protein research Pub Date : 1993-05-01

W F Schmidt, R M Waters, A D Mitchell, J D Warthen, I L Honigberg, H Van Halbeek

引用次数: 0

Methionine-enkephalin related glycoconjugates. Synthesis and biological activity. 蛋氨酸-脑啡肽相关糖缀合物。合成及生物活性。

International journal of peptide and protein research Pub Date : 1993-04-01

S Horvat, J Horvat, L Varga-Defterdarović, K Pavelić, N N Chung, P W Schiller

引用次数: 0

Molecular design of peptides. Synthesis, molecular structure and beta-turn II' formation of N-Boc-L-Phe-dehydro-Abu-NH-CH3 in crystals. 肽的分子设计。n - boc - l - ph -脱氢- abu - nhh - ch3晶体的合成、分子结构和β -turn II'形成。

International journal of peptide and protein research Pub Date : 1993-04-01

T P Singh, P Narula

{"title":"Molecular design of peptides. Synthesis, molecular structure and beta-turn II' formation of N-Boc-L-Phe-dehydro-Abu-NH-CH3 in crystals.","authors":"T P Singh, P Narula","doi":"","DOIUrl":"","url":null,"abstract":"The peptide N-Boc-L-Phe-dehydro-Abu-NH-CH3 was synthesized by the usual workup procedure. The crystals grown from methanol at 4 degrees C belong to the space group P2(1)2(1)2(1) with a = 7.589(2), b = 13.690(4), c = 21.897(6) A, Z = 4 and dc = 1.149(5) g cm-3 for C19H29N3O5.CH3OH. The peptide crystals were highly sensitive to radiation. The final agreement factor R was 0.055 for 1109 observed reflections (I > or = 2 sigma) with data extending to a 2 theta value of 103 degrees. The methanol oxygen atom is split into two occupancies. Both sites are involved in identical hydrogen bonding. As a result of substitution of a dehydro-Abu residue at the (i + 2) position the peptide adopts an ideal beta-turn II' conformation with torsion angles of corner residues as phi 1 = 63(1) degrees, psi 1 = -127(1) degrees, phi 2 = -66(1) degrees and psi 2 = -10(1) degrees, and an intramolecular hydrogen bond N-H...O of length 3.01(1) A. This shows that the conformational constraints produced by dehydro-Abu are similar in nature to but different in magnitude than those produced by dehydro-Phe and dehydro-Leu. The methanol-peptide interactions show characteristic features of multiple hydrogen-bond formations involving polar sites of participating peptide and methanol molecules. The packing of the molecules in the unit cell is stabilized by interactions through methanol molecules with the help of several hydrogen bonds.","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"41 4","pages":"394-8"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19479020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0