International Journal of Health, Animal science and Food safety最新文献

{"title":"Morphological markers to select populations of oocytes with different cultural needs for dedicated pre-maturation protocols","authors":"C. Dieci, V. Lodde, Rémi Labreque, I. Tessaro, M. Sirard, A. M. Luciano","doi":"10.13130/2283-3927/8367","DOIUrl":"https://doi.org/10.13130/2283-3927/8367","url":null,"abstract":"Oocyte’s chromatin gradually becomes more compacted during the final stage of oocyte development and the level of chromatin compaction is considered a marker of oocyte differentiation [Luciano et al, 2014]. Moreover, several studies demonstrate that in vitro pre-maturation treatments (Pre-IVM), aimed to improve the developmental capability of immature oocytes, might behave differently depending on the oocyte metabolic status, when it is isolated from follicle [Luciano et al., 2011]. This study aims at identifying correlations between cumulus-oocyte complex (COC) morphology and oocyte chromatin configuration and secondly at testing the hypothesis that only fully grown oocytes at earlier stages of differentiation with loosely compacted chromatin  (GV1) can benefit from Pre-IVM treatment. COCs were collected from bovine 2-6mm ovarian follicles, and further divided in three groups according to their morphology (Class-1, 2 and 3) as previously described [Blondin & Sirard, 1995]. Analysis of chromatin configuration revealed that only Class-1 COC was enriched in GV1 oocyte, while Class-2 and 3 presented a similar distribution of GV1, GV2 and GV3 oocytes, where GV2 and 3 oocytes are characterized by increased chromatin compaction [Lodde et al., 2007]. Then COCs were divided into two groups, one containing Class-1 COCs and the other containing Class-2 and 3 COCs and subjected to pre-IVM for 6 hours in presence of cilostamide and 10-4 UI/ml rhFSH. Finally, COCs underwent standard in vitro maturation (IVM) for 22 hours, in vitro fertilization and embryo culture. Blastocyst rate and embryos cell number were assessed at day 7. Pre-IVM positively affected developmental competences of Class-1, while in Classes 2 and 3 Pre-IVM had detrimental effects.In conclusion COCs morphology could be used as a non-invasive approach to select population of oocyte with different cultural needs. These data could be useful in setting-up dedicated IVM protocols considering specific genes and pathways to improve IVP efficiency.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91405203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Analysis of MicroRNAs in FFPE samples of canine cutaneous and oral melanoma by RT-qPCR","authors":"C. Catozzi, C. Lecchi, C. Gini, F. Ceciliani, R. Finotello, L. Ressel","doi":"10.13130/2283-3927/8374","DOIUrl":"https://doi.org/10.13130/2283-3927/8374","url":null,"abstract":"MicroRNA (miRNA), a class of small, non-coding RNA - regulating post-transcriptionally protein expression - are emerging as clinical biomarkers in many pathologies, including cancer [1]. Since miRNA are supposed to represent fundamental key regulators, better understanding of melanoma biology is essential to improve staging and therapy. The aim of the study was to investigate whether miRNA expression can vary in canine melanoma samples derived from formalin-fixed-paraffin-embedded (FFPE) tissues. Experimental design of the study included three groups, each one composed of 7 animals: i) control healthy skin group ii) oral melanoma group iii) skin melanoma group. The histhopatology and immunoistochemistry details of dogs included in the study are previously reported  [2]. Two tissue slides were used for miRNA extraction. The expression levels of seven miRNA - miR-145, miR-146a, miR-425-5p, miR-223, miR-365, miR-155 and miR-134 - were detected and assessed by qPCR using TaqMan® probes [3-5]. Five miRNA were significantly up-(n=3) or down-(n=2) regulated. In details, miR-146a and miR-155 abundance was increased as compared with control in both oral and skin melanoma (Fig 1 B,E) ( p = 0.004 and 0.014 and p = 0.043 and 0.035 respectively), while the levels of miR-145 and miR-365 were lower (Fig 1 A,D) ( p = 0.018 and 0.008 and p = 0.01 and 0.028, respectively). MiR-425-5p was upregulated ( p = 0.039) only in skin melanoma (Fig. 1 C). Furthermore, functional analysis, carried out using miRNet web-based tool, showed that 76 genes related to cancer-associated pathways were possible target of these five microRNA ( p = 6.95E-9); in particular, 21 target genes were associated with melanoma ( p = 1.47E-5), including BRAF and CDK, E2F, FGF and PIK3 families. In conclusion, miR-145, miR-146a, miR-425-5p, miR-365 and miR-155 are differentially expressed in melanoma and healthy FFPE samples, suggesting that they may play a role in canine melanoma pathogenesis and/or progression.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75163019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-guided epidural catheter placement with a new technique: preliminary cadaveric study.","authors":"E. D'urso, S. Faverzani, G. Barella, F. D. Cesare, D. Gioeni, V. Rabbogliatti, D. Stefanello, G. Ravasio","doi":"10.13130/2283-3927/8389","DOIUrl":"https://doi.org/10.13130/2283-3927/8389","url":null,"abstract":"Several methods are described in veterinary medicine to perform and assess correct epidural needle placement to provide effective epidural analgesia (Adami et al 2017). The aim of this study is to evaluate the feasibility of an ultrasound longitudinal sagittal approach to epidural catheter placement using a biopsy needle guide. Seven dog cadavers were used in the study. With the cadaver in sternal recumbence, a 5-8 MHz microconvex transducer provided with a 16-gauge biopsy guide was positioned to obtain a longitudinal sagittal scan of the spinal process of L7 and the sacral crest; the epidural space was identified between two parallel hyperechoic lines and, as the trajectory of the biopsy guide crossed them, a 17G Tuohy needle was used to insert a 19G epidural catheter. Correct catheter placement was visualised through a resection of the column between L2 and L3. Firstly, an expert echographist (operator C1) visualised the ultrasonographic landmarks, while catheter placement was performed by an expert anaesthetist (operator A), a student (operator B) and another expert echographist (operator C2) (double-operator technique); secondly, operator A and C2 performed alone the whole procedure (single-operator technique); lastly all operators performed a blind procedure (Jones 2001). Operator A failed 2/7 single-operator procedures; time to perform the blind technique was statistically lower than the double-operator technique (75 ± 132.4 vs 91.6 ± 79.3 seconds). Operator C2 failed 3/7 blind procedures, scoring the higher total time of performance (329.3 ± 271.2 seconds), but was able to perform both the double- and single-operator technique without significant difference with operator A, despite a faster time in positioning the probe. Operator B showed a higher repositioning attempts of the needle with the double-operator procedure compared to the blind one. Ultrasound guidance appears to be a promising technique to ease catheter placement also by operators inexperienced of locoregional techniques.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74573574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical composition of amniotic fluid in normal puppies at term of pregnancy: preliminary data","authors":"B. Bolis, M. Schrank, J. Fusi, A. Mollo, M. Veronesi","doi":"10.13130/2283-3927/8343","DOIUrl":"https://doi.org/10.13130/2283-3927/8343","url":null,"abstract":"The full knowledge of the normal fetal fluids composition could be useful in the dog for the better management of newborns. The aim of the present study was to define the biochemical composition of amniotic fluid of puppies born by elective Caesarean section (CS) at term of pregnancy. The study enrolled 24 purebred bitches, classified into small size ( 20kg). All the bitches were healthy and clinically monitored from mating until parturition. For all the bitches an elective CS at term of pregnancy was performed [1]. For each puppy, the amniotic fluid was collected, immediately centrifuged and frozen at – 20° C until analysis for ALB, AMY, TB, CHOL, CK, ALP, GGT, AST, ALT, LDH, Mg, Ca, K, Na, Trig, BUN, Glc, TP, CREA, LIP, Cl, and GLOB. Data were analyzed by ANCOVA to verify the possible effects of parity, breed body size and newborn gender on amniotic biochemical composition. A total of 69 amniotic fluid samples were collected. The amniotic mean±SD and min-max values for each parameter were defined. LDH (p<0.01) and CK activity (p<0.05), as well as Glc concentrations (p<0.0001) were negatively influenced by the parity. AMY activity was significantly (p<0.05) higher in large sized (44.2±20.87 U/L) respect to small sized dogs (30.3±19.89 U/L), while lower (p<0.05) CHOL amniotic concentrations were found in small sized (3.0±2.71 mg/dl) as compared to large sized (3.9±2.93 mg/dl) dogs. Gender of the newborn did not influence the amniotic biochemical composition. The preliminary results of this study showed some similarities as well as some differences concerning the biochemical composition of the amniotic fluid in dogs at term of pregnancy if compared to data reported for the cat [2]. Furthermore, the results suggested that, in dogs, some amniotic parameters could be influenced by breed body size and by parity.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82920613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing of Staphylococcus pseudintermedius canine strains by three commonly used techniques","authors":"G. Meroni, S. Morandi, M. Brasca, P. Martino","doi":"10.13130/2283-3927/8375","DOIUrl":"https://doi.org/10.13130/2283-3927/8375","url":null,"abstract":"Staphylococcus pseudintermedius is a newly described species of Staphylococcus regarded as the main causative agent of canine pyoderma [1]. S. pseudintermedius infection was recently described in humans. An important feature of this pathogen is the high genetic identity with two other species of staphylococci, namely S. intermedius and S. delphini , which are included all together in the Staphylococcus Intermedius Group (SIG) [2]. This scenario seriously hampers phenotypic differentiation of these three pathogens. Despite this, only in 2008 was described the first molecular protocol for diagnostic identification of S. pseudintermedius [3]. The aim of this work was to investigate the presence of different biotypes of S. pseudintermedius obtained from clinically relevant cases of pyoderma in dogs using three molecular methods commonly used to type bacteria: the Ribosomal Spacers Amplification (RSA), the Random Amplification of Polymorphic DNA (RAPD) and the Restriction Fragment Length Polymorphism (RFLP). A total of 46 different strains were included in this work. The application of the RSA technique, which was applied here for the first time, identified the presence of S. pseudintermedius , although it did not allow any differentiation between biotypes. The RAPD assay showed a single cluster that assembles all the interested strains that are grouped in three different sub-clusters ( Fig. 1 ). The RFLP technique showed the most discriminative power, providing the opportunity to clearly identify this bacterium. In conclusion, the use of these three different techniques allows to clearly identify S. pseudintermedius and to observe the presence of different biotypes. In future it could be interesting to couple these results with the determination of the antibiotic resistance in order to verify if certain Multi Drug Resistant strains have particular RSA and RAPD profiles.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77706459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary natural extracts mixture on rabbit does reproductive performances: preliminary data.","authors":"S. Chiapparini, F. Vizzarri, C. Corino, R. Rossi, G. Maiorano","doi":"10.13130/2283-3927/8365","DOIUrl":"https://doi.org/10.13130/2283-3927/8365","url":null,"abstract":"In the recent years, research has focused the attention on the use of substances able to improve both animal health and meat quality. The objective of this study was to investigate the effect of dietary supplementation with natural extracts in rabbit does on reproductive parameters. Natural extracts have been widely reported to have potent antioxidant, anti-inflammatory and antimicrobic activities related especially to their phenolic content (Pereira et al., 2009). In rabbit production, does are normally inseminated 11 days after parturition, with kits being weaned at 35 days (Rebollar et al., 2006). The reproductive phase is a critical period because the animals have few days to reconstitute their body reserves (Castellini et al., 2003).  Therefore, the interaction between nutrition and reproduction is crucial in this specie (Butler, 2000; Roche et al., 2000). The trial was performed at the Research Institute for animal production in Nitra (Slovak Republic). Sixty does were randomly selected, artificially inseminated and divided into three experimental groups. The first fed a basal diet (C), the second one (T1) received 0.3% of natural extracts mixture and the third one (T2) an integration of 0.6% of natural extracts mixture for gestation and lactation period (65 days) The animals were allocated in individual flat-deck cages with a closeable nest box. At parturition, the total number of kits born and stillborn and litters weights were recorded. The data were analyzed by one way Analysis of Variance (ANOVA) using SPSS (IBM.-SSPS Statistics 24). Dietary supplementation did not affect (P>0.05) number of kids born (8.0 ± 1.0 C vs 7.3 ± 0.97 T1 and 7.4 ± 1.0 T2) and birth weight (63 ± 2.0 g C vs 60.1 ± 2.3 g T1 and 61.0 ± 2.4 g T2).  The administration of natural extract in does did not improve (P>0.05) the kits average daily gain (20.54 ± 1.3 g/d C vs 21.92 ± 0.5 g/d T1 and 20.93 ± 0.9 g/d T2) and body weight at weaning (829 ± 16.6 g C vs 834 ± 26.6 g T1 and 826 ± 26.8 g T2). These preliminary data showed that at the present dosage, the natural extracts mixture is not able to affect does reproductive performance. However, further research is needed to confirm the present data and explore the mechanism of action of this natural mixture.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79941298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyses of organochlorine pesticides residues in eels (Anguilla anguilla) from Lake Garda using Gas chromatography coupled with Tandem Mass Spectrometry (GC-MS/MS).","authors":"G. Labella, L. Chiesa, S. Panseri, F. Arioli","doi":"10.13130/2283-3927/8377","DOIUrl":"https://doi.org/10.13130/2283-3927/8377","url":null,"abstract":"Lake Garda is located in Insubria region, that is known for being the most populated and industrialized area of Italy (Camusso et al., 2001). Therefore, the Lake water, and also the fish species present, could be affected by environmental contamination.  European eel ( Anguilla anguilla ) are considered as suitable matrix for biomonitoring environmental contaminants in European water (Belpaire et al., 2007), being widespread in many European waters and highly contaminated by lipophilic compounds, due to the high lipid content (up to 40%) (Larsson et al., 1991). Moreover, eel is an edible species (its farming currently supplies approximately 45,000 tons/year) (Nielsen et al., 2008), so it also represents a public health issue. Based on these considerations, the aim of this study was to evaluate the occurrence of fourteen organochlorine pesticides (OCs) in forty-five eels ( Anguilla anguilla ) from Lake Garda, using Accelerated Solvent Extraction (ASE) procedure for the analytes extraction and Gas chromatography coupled with Tandem Mass Spectrometry (GC-MS/MS) for the analysis of OCs. GC-MS/MS analysis was developed and validated according to the SANTE/11945/2015 guidelines.  Uncontaminated eel sample (previously checked for the presence of OCs and considered blank with a concentration of compounds < Limit of Detection) were used for all procedure's optimization steps. For all the OCs analysed, satisfactory results were achieved. Regarding eel samples, several pesticides were detected, but DDTs (DDT and its metabolites) were found with the highest prevalence (92 %). The concentration rage was from not detected (n.d.) to 19000 ng g -1 . Although DDTs levels in the environment are declining (Albaiges et al., 2011), they continue to bioaccumulate in tissues of human and animal and biomagnify in food chains.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84140261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary study about the detection of perfluoroalkyl substances in eel samples of Lake Garda by liquid chromatography tandem mass high resolution mass spectrometry (LC-HRMS).","authors":"M. Nobile, S. Panseri, F. Arioli, L. Chiesa","doi":"10.13130/2283-3927/8376","DOIUrl":"https://doi.org/10.13130/2283-3927/8376","url":null,"abstract":"Perfluoroalkyl substances (PFASs) are a large class of fluorinated aliphatic chemical of anthropogenic origin with high chemical stability even at high temperatures and in presence of alkalis, strong acids or oxidizing agents (Lau et al. 2004). All these characteristics make them no biodegradable and very persistent in the environment, associated with adverse health risks (Eriksen et al. 2010). Food, especially fish and other seafood, is considered the main source of exposure to PFASs (EFSA 2012). In this preliminary study we developed and validated a sensitive, selective and specific method by LC-HRMS Orbitrap to monitor the presence of 16 PFASs in eel ( Anguilla Anguilla ) samples. The clean-up of the lyophilized samples consisted of a previous extraction step with acetonitrile to precipitate also proteins, followed by a purification step through Oasis® WAX SPE (Weak Anionic Exchange Solid Phase Extraction) cartridges. The method applied to 45 farmed eel samples from Lake Garda showed the presence of several PFASs, up to 10 in the same eel, in the order of ng/g (Fig.1). The results provided a representative situation of the  PFASs contamination level of the lake, lower than those of others European countries (Hoff et al. 2005, Kwadijk et al. 2010).","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90292224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatty acid profile, Desaturase and Atherogenic indices in milk of Holstein Friesian and Italian autochthonous cattle breeds","authors":"G. Curone, S. Panseri, C. Colombani, L. Chiesa, J. Filipe, M. Faustini","doi":"10.13130/2283-3927/8403","DOIUrl":"https://doi.org/10.13130/2283-3927/8403","url":null,"abstract":"In the past decades, milk has been considered a mere supplier of nutrients, although its importance was considered paramount for the development and growth of newborns, a number of aspects regarding the biological functions of milk are still unknown. Several positive functional properties of milk derive from fatty acids (FA), mainly unsaturated fatty acids (UFA), either monounsaturated (MUFA) or polyunsaturated (PUFA) fatty acids. In particular, UFAs are considered functional components of food because of their positive effects on disease prevention (FAO, 2010; Connor W.E. ,2000;  Wijendran V., 2004). The objective of this study was to characterize the fatty acid profile, the desaturase index and the atherogenic index in milk of local Italian bovine breeds (Cabannina, Varzese and Valdostana) and in a cosmopolitan breed (Holstein Friesian) during the first period of lactation. A total number of 129 cows have been enrolled (Friesian n=30, Cabannina n=30, Varzese n=30, Valdostana n=39) from three dairy farms with similar management and feeding conditions. Animals were chosen in order to have three classes of lactation stage: milk collections were carried out starting from 40±10 days (group A), 70±10 days (group B), and 130±10 days (group C). Milk samples have been analyzed by gas chromatography to obtain the fatty acid profile, on the basis of these results, the Desaturase and Atherogenic Indices were calculated. A   number   of   differences   between   breeds have been  found, in   particular local  breeds showed an higher  percentages  of  UFA,  MUFA,  PUFA,  and  a  higher  UFA/SFA  ratio, as well as lower desaturase indices (related to C14, C16 and C18) and atherogenic index, when compared to Friesian cows. The results can add further information aiming to re-evaluate an almost lost local treasure in Northern Italy.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76125395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gut microbiome and mucosal defenses in cats with coronaviruses: a pilot study","authors":"S. Meazzi, S. Lauzi, A. Stranieri, S. Paltrinieri, A. Giordano","doi":"10.13130/2283-3927/8410","DOIUrl":"https://doi.org/10.13130/2283-3927/8410","url":null,"abstract":"Feline Infectious Peritonitis (FIP) develops from a mutation of enteric feline coronaviruses (FCoVs) and an imbalance of the host immune response. The wide polymorphism of FCoVs is associated with the viral replication rate (Licitra et al. 2013) . Changes in the composition of the gut microbiota may induce quali-quantitative modifications in FCoVs and/or different immune profiles (Weese et al., 2015). Few information is available on feline gut microbiome and the association between microbiota and the predisposition to pathological conditions (Ramadan et al., 2014). The aim of this study is to provide preliminary data about the composition of gut microbiota in healthy cats compared with FCoV infected cats (with and without  FIP), in order to evaluate whether changes of gut microbiota may induce changes in FCoV, in its genetic polymorphism and in the mucosal immunity. Screening analyses have been performed on 22 cats: - Routine hematology and biochemistry on EDTA and serum (included electrophoresis and alpha-1-acid glycoprotein measurement for cats suspected with FIP) - Nested RT-PCR-3’UTR on frozen faeces - Effusion evaluation - FIV/FeLV serology Due to strict inclusion criteria (cats younger than 2.5 years old, indoor and not assuming antibiotics in the previous two months) and based on the results obtained from the complete set of analysis, only 15 cats, specifically 5 cats for each of the following 3 groups: FIP- affected, healthy negative and positive for FCoV, have been recruited to perform the following analyses:  - microbiota analysis through NGS of 16S rRNA gene (V4 region) amplicons followed by bioinformatic analysis  -  evaluation of secretory IgA (ELISA kit) - phylogenetic analysis of FCoVs S gene sequences Innovative results will be provided on the feline gut microbiota composition. These will be correlated with the presence and genetic polymorphisms of FCoV and mucosal defenses to establish significant correlations between the analysed factors.","PeriodicalId":14105,"journal":{"name":"International Journal of Health, Animal science and Food safety","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85181226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}