Insect Biochemistry最新文献_第7页

Physiological actions by hypertrehalosemic hormone and adipokinetic peptides in adult Blaberus discoidalis cockroaches 高海藻素和脂肪动力学肽对盘状小蠊成虫的生理作用

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90041-C

L.L. Keeley, T.K. Hayes, J.Y. Bradfield, S.M. Sowa

{"title":"Physiological actions by hypertrehalosemic hormone and adipokinetic peptides in adult Blaberus discoidalis cockroaches","authors":"L.L. Keeley, T.K. Hayes, J.Y. Bradfield, S.M. Sowa","doi":"10.1016/0020-1790(91)90041-C","DOIUrl":"10.1016/0020-1790(91)90041-C","url":null,"abstract":"<div>Members of the adipokinetic hormone/red pigment-concentrating hormone (AKH/RPCH) family characteristically cause metabolite mobilization by the insect fat body. The present study identified several additional physiological actions in adult Blaberus discoidalis cockroaches that were influenced by synthetic Blaberus hypertrehalosemic hormone (HTH) and other AKH/RPCH family peptides. HTH elevated blood carbohydrate by 4-fold and cytochrome heme a + b synthesis of fat body mitochondria by 3-fold. Both carbohydrate and heme synthesis were dose-responsive to HTH. Carbohydrate synthesis was 10 times more sensitive to HTH than heme synthesis. Heme synthesis was also stimulated by Periplaneta cardioacceleratory hormones (CAH)-I and -II and RPCH but not by AKH-I or -II, at the doses tested. HTH showed strong cardioexcitatory activity. Long-term treatment of decapitated female B. discoidalis with juvenile hormone analog (JHA = methoprene) stimulated by 2.6-fold the rate of synthesis of secreted fat body proteins. HTH enhanced the JHA-dependent export protein synthesis by 42% above that observed with JHA alone. SDS-PAGE demonstrated that JHA determined the nature of the newly synthesized polypeptides; HTH enhanced their synthesis rate. Neither AKH-I nor HTH affected protein synthesis when added directly to isolated fat body. These results demonstrate that peptides of the AKH/RPCH family have multiple physiological actions related to fat body energy metabolism.</div>","PeriodicalId":13955,"journal":{"name":"Insect Biochemistry","volume":"21 2","pages":"Pages 121-129"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-1790(91)90041-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90343049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

International Symbiosis Congress 国际共生大会

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90071-L

引用次数: 0

Biosynthesis and catabolism of insect hormones and pheromones 昆虫激素和信息素的生物合成和分解代谢

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90096-W

引用次数: 0

Vitellogenin synthesis, processing and hormonal regulation in the tick, Ornithodoros parkeri (Acari:Argasidae) 蜱体内卵黄蛋白的合成、加工和激素调节

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90113-S

DeMar Taylor, Yasuo Chinzei, Ken Miura, Katsuhiko Ando

引用次数: 51

Salivary amino acids in Lygus species (Heteroptera:Miridae) Lygus物种唾液氨基酸的研究（异翅目：Miridae）

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90117-W

Seppo Laurema, Anna-Liisa Varis

引用次数: 6

Development of functionally competent cabbage looper moth sex pheromone glands 功能性环蛾性信息素腺体的发育

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90027-C

Juliet D. Tang, Walter A. Wolf, Wendell L. Roelofs, Douglas C. Knipple

{"title":"Development of functionally competent cabbage looper moth sex pheromone glands","authors":"Juliet D. Tang, Walter A. Wolf, Wendell L. Roelofs, Douglas C. Knipple","doi":"10.1016/0020-1790(91)90027-C","DOIUrl":"10.1016/0020-1790(91)90027-C","url":null,"abstract":"<div>Unlike some moths, pheromone production in Trichoplusia ni is not regulated by a pheromone activating neuropeptide. Rather, competency to produce pheromone apparently is linked with changes in the ecdysteroid titer that occur late in metamorphosis. In contrast to adult pheromone glands, glands from pharate adults 2 days before eclosion were non-competent, and (1) had undetectable levels of the pheromone, (Z)-7-dodecenyl acetate, and pheromone-specific intermediates, (2) showed little or no conversion of radiolabeled substrate to product in enzyme assays of fatty acid synthetase, Δ11 desaturase, and acetyltransferase, and (3) failed to incorporate radiolabeled acetate into pheromone in gland culture. Glands 1 day before adult eclosion exhibited low titers of pheromone and the intermediate, (Z)-11-hexadecenoate, and showed low levels of radiolabeled acetate incorporation into pheromone in gland culture. By the time of adult eclosion, the gland was fully competent. Precocious development of pheromone gland competency was induced by removing the head and thorax from pupae 2 days before adult eclosion. This effect appears to result from the reduction of ecdysteroid, since it was blocked by the administration of 20-hydroxyecdysone. This ability to manipulate the development of the pheromone gland was restricted to a critical period, since removal of head and thorax from younger pupae did not induce pheromone gland competency, and administration of 20-hydroxyecdysone to older pupae did not block its onset. In addition to differences in competency, early pharate and adult glands exhibited dissimilarities with respect to (1) the types of proteins synthesized in gland culture, and (2) the types of proteins translated from mRNA in vitro.</div>","PeriodicalId":13955,"journal":{"name":"Insect Biochemistry","volume":"21 6","pages":"Pages 573-581"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-1790(91)90027-C","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72680702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

The Leucophaea maderae hindgut preparation: A rapid and sensitive bioassay tool for the isolation of insect myotropins of other insect species 马氏白蛉后肠制剂:一种快速、灵敏的分离其他昆虫类促肌蛋白的生物测定工具

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90070-U

G.M. Holman , R.J. Nachman , L. Schoofs , T.K. Hayes , M.S. Wright , A. DeLoof

{"title":"The Leucophaea maderae hindgut preparation: A rapid and sensitive bioassay tool for the isolation of insect myotropins of other insect species","authors":"G.M. Holman , R.J. Nachman , L. Schoofs , T.K. Hayes , M.S. Wright , A. DeLoof","doi":"10.1016/0020-1790(91)90070-U","DOIUrl":"10.1016/0020-1790(91)90070-U","url":null,"abstract":"<div>The isolated hindgut preparation of the cockroach, Leucophaea maderae has provided an effective bioassay tool for the isolation of certain structural types of insect myotropic peptides. Initially, the preparation was used to monitor excitatory and inhibitory activities of numerous HPLC fractions in a study that resulted in the structural characterization of 12 Leucophaea neuropeptides. Subsequently, the preparation was used as the bioassay for the isolation and structural characterization of myotropic neuropeptides of the house cricket, Acheta domesticus, and the locust, Locusta migratoria. Five novel myotropic peptides from the cricket were structurally characterized, and 32 separate myotropic compounds were isolated from nervous tissue of the locust. At present, 8 of the locust peptides have been structurally characterized. Isolation studies using this bioassay have been responsible for the discovery of 25 unique neuropeptides, 4 new peptide families, and the initial demonstration of the natural analog phenomenon in insects.</div>","PeriodicalId":13955,"journal":{"name":"Insect Biochemistry","volume":"21 1","pages":"Pages 107-112"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-1790(91)90070-U","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73427783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 129

Isolation and complete amino acid sequences of eclosion hormones of the silkworm, Bombyx mori 家蚕羽化激素的分离及氨基酸序列分析

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90049-K

Takaharu Kono , Hiromichi Nagasawa , Akira Isogai , Hajime Fugo , Akinori Suzuki

引用次数: 11

Purification and characterization of an ovulation stimulating substance from the male accessory glands of Drosophila suzukii 苏氏果蝇雄性副腺促排卵物质的纯化及特性研究

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90008-3

Yuko Y. Ohashi, Kazu Haino-Fukushima, Yoshiaki Fuyama

引用次数: 43

Characterization of the isoforms of phospholipase A2 from honeybee venom 蜂毒磷脂酶A2亚型的研究

Insect Biochemistry Pub Date : 1991-01-01 DOI: 10.1016/0020-1790(91)90099-Z

Friedrich Altmann , Viktoria Kubelka , Erika Staudacher , Karola Uhl , Leopold März

{"title":"Characterization of the isoforms of phospholipase A2 from honeybee venom","authors":"Friedrich Altmann , Viktoria Kubelka , Erika Staudacher , Karola Uhl , Leopold März","doi":"10.1016/0020-1790(91)90099-Z","DOIUrl":"10.1016/0020-1790(91)90099-Z","url":null,"abstract":"<div>Phospholipase A2 from the venom of the European honeybee (Apis mellifera) consists of three isoforms with approximate molecular masses of 16, 18, and 20 kDa, respectively, as deduced from SDS-PAGE. These variants, termed PLA-16, PLA-18, and PLA-20, were isolated by lectin affinity chromatography and preparative polyacrylamide gel electrophoresis. The amino acid sequences of the N-terminal peptide portions of all three isoforms, as assessed by automated Edman degradation, were identical with that expected for honeybee phospholipase A2. Sequencing data suggest that, while PLA-18 and PLA-20 carry oligosaccharide residues at asparagine-13, PLA-16 has escaped glycosylation during biosynthesis. Release of the carbohydrate from PLA-18 and PLA-20 with peptide: N-glycosidase F abolished the molecular mass differences between the three isoforms of phospholipase. Differences in sensitivity to α-mannosidase and monosaccharide composition of PLA-18 and PLA-20 further indicate that their electrophoretic separation is based on structural features of the N-glycosidically linked oligosaccharide. Noticeably, PLA-20 contains N-acetylgalactosamine, a sugar not having yet been described as a constituent of insect glycoproteins.</div>","PeriodicalId":13955,"journal":{"name":"Insect Biochemistry","volume":"21 5","pages":"Pages 467-472"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-1790(91)90099-Z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80702018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19