International Journal of Analytical Chemistry最新文献

{"title":"Structure Elucidation of a Novel Polysaccharide Isolated from Euonymus fortunei and Establishing Its Antioxidant and Anticancer Properties","authors":"Yu Luo, Hongtao Chen, Chunxi Huang, Shujia He, Qilong Wen, Danzhao Cai","doi":"10.1155/2024/8871600","DOIUrl":"https://doi.org/10.1155/2024/8871600","url":null,"abstract":"<i>Euonymus</i><i>fortunei</i> polysaccharides (EFPs) have not been extensively investigated yet in terms of their extraction and biological activity. The orthogonal experimental design was employed in this study to evaluate the optimum yield of EFPs. A maximum yield of 2.63 ± 0.23% was attained using material-liquid ratios of 60 mL/g, extraction temperature of 80°C, ultrasonic power of 144 W, and extraction time of 75 mins. The polysaccharide content reached 53.47 ± 0.31% when deproteinized thrice. An analysis of monosaccharide composition revealed that these polysaccharides consist of Gal, Glc, Man, Fuc, and Rha with a molar ratio of 7.14 ∶ 23.99 ∶ 6.29 ∶ 6.55 ∶ 1.00, respectively, in EFPs. Subsequently, the <i>in vitro</i> scavenging capacities of 2,2-diphenylpicrylhydrazyl (DPPH) and ·OH and superoxide anion radicals, along with the reducing power of EFPs, were studied. Results revealed that EFPs have higher antioxidant activity, particularly ·OH scavenging, as well as reducing power, as compared to <i>Astragalus</i> polysaccharides (ASPs) and <i>Lycium barbarum</i> polysaccharides (LBPs). The Cell Counting Kit-8 (CCK-8) method was used to evaluate the effects of different concentrations of polysaccharides on SKOV3 cell proliferation, and the results revealed their inhibition at concentrations in the range of 200–800 <i>μ</i>g/mL. In addition, findings from flow cytometry further confirmed that EFPs blocked the cell cycle at G0/G1 and S phases and induced SKOV3 cell apoptosis. In a word, EFPs could be exploited and used further based on the experimental results from this study.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"9 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141147546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorption Characteristics and Chromatographic Separation of 90Y3+ from 90Sr2+ from Aqueous Media by Chelex-100 (Anion Ion Exchange) Packed Column","authors":"Mohammad S. El-Shahawi, Hassan Alwael, Abdulaziz A. Alsibaai, Abdelgany Hamza, Faisal K. Algethami, Fatmah M. Alshareef, Sanaa H. El-Khouly, Neven Eweda","doi":"10.1155/2024/6232381","DOIUrl":"https://doi.org/10.1155/2024/6232381","url":null,"abstract":"There is growing demand for separation of ⁹⁰Y carrier free from ⁹⁰Sr coexisting to produce high purity ⁹⁰Y essential for radiopharmaceutical uses. Thus, in this context the sorption profiles of Y³⁺ and Sr²⁺ from aqueous solutions containing diethylenetriaminepenta acetic acid (DTPA), ethylenediaminetetra-acetic acid (EDTA), acetic acid, citric acid, or NaCl onto Chelex-100 (anion ion exchange) solid sorbent were critically studied for developing an efficient and low-cost methodology for selective separation of Y³⁺ from Sr²⁺ ions (1.0 × 10⁻⁵ M). Batch experiments displayed relative chemical extraction percentage (98 ± 5.4%) of Y³⁺ from aqueous acetic acid solution onto Chelex-100 (anion ion exchanger), whereas Sr²⁺ species showed no sorption. Hence, a selective separation of Y³⁺ from its parent ⁹⁰Sr²⁺ has been established based upon percolation of the aqueous solution of Y³⁺ and Sr²⁺ ions containing acetic acid at pH 1-2 through Chelex-100 sorbent packed column at a 2 mL min⁻¹ flow rate. Y³⁺ species were retained quantitatively while Sr²⁺ ions were not sorbed and passed through the sorbent packed column without extraction. The sorbed Y³⁺ species were then recovered from the sorbent packed column with HNO₃ (1.0 M) at a 1.0 mL min⁻¹ flow rate. A dual extraction mechanism comprising absorption associated to “weak-base anion exchanger” and “solvent extraction” of Y³⁺ as (YCl₆)³⁻ and an extra part for “surface adsorption” of Y³⁺ by the sorbent is proposed. The established method was validated by measuring the radiochemical (99.2 ± 2 1%), radionuclide purity and retardation factor (<svg height=\"14.1649pt\" style=\"vertical-align:-5.529pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 14.5964 14.1649\" width=\"14.5964pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,8.086,3.132)\"></path></g></svg> = 10.0 ± 0.1 cm) of ⁹⁰Y³⁺ recovered in the eluate. Ultimately, the sorbent packed column also presented high stability for reusing 2-3 cycles without drop in its efficiency (±5%) towards Y³⁺ uptake and relative chemical recovery. A proposed flow sheet describing the analytical procedures for the separation of ⁹⁰Y³⁺ from ⁹⁰Sr²⁺ using chelating Chelex 100 (anion exchange) packed column is also included.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"64 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic Properties of Baitouweng Decoction in Bama Miniature Pigs: Implications for Clinical Application in Humans","authors":"Qianqian Xu, Huilan Gao, Fuqiang Zhu, Wenliang Xu, Yubo Wang, Jinwen Xie, Guangjun Guo, Limei Yang, Li Ma, Zhiqiang Shen, Jichang Li","doi":"10.1155/2024/5535752","DOIUrl":"https://doi.org/10.1155/2024/5535752","url":null,"abstract":"Traditional Chinese medicine (TCM) serves as a significant adjunct to chemical treatment for chronic diseases. For instance, the administration of Baitouweng decoction (BTWD) has proven effective in the treatment of ulcerative colitis. However, the limited understanding of its pharmacokinetics (PK) has impeded its widespread use. Chinese Bama miniature pigs possess anatomical and physiological similarities to the human body, making them a valuable model for investigating PK properties. Consequently, the identification of PK properties in Bama miniature pigs can provide valuable insights for guiding the clinical application of BTWD in humans. To facilitate this research, a rapid and sensitive UPLC-MS/MS method has been developed for the simultaneous quantification of eleven active ingredients of BTWD in plasma. Chromatographic separation was conducted using an Acquity UPLC HSS T3 C₁₈ column and a gradient mobile phase comprising acetonitrile and water (containing 0.1% acetic acid). The methodology was validated in accordance with the FDA Bioanalytical Method Validation Guidance for Industry. The lower limit of quantitation fell within the range of 0.60–2.01 ng/mL. Pharmacokinetic studies indicated that coptisine chloride, berberine, columbamine, phellodendrine, and obacunone exhibited low <span><svg height=\"11.9316pt\" style=\"vertical-align:-3.2957pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 25.3434 11.9316\" width=\"25.3434pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,8.619,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,16.181,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,20.267,3.132)\"></path></g></svg>,</span> while fraxetin, esculin, fraxin, and pulchinenoside <svg height=\"11.5695pt\" style=\"vertical-align:-3.18152pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.38798 12.7187 11.5695\" width=\"12.7187pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,7.644,3.132)\"></path></g></svg> were rapidly absorbed and eliminated from the plasma. These findings have implications for the development of effective components in BTWD and the adjustment of clinical dosage regimens.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"124 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Analysis of Leontopodium leontopodioides (Willd.) Beauv. Chemical Composition by GC/MS and UPLC-Q-Orbitrap MS","authors":"Yuanyuan Chen, Yu Dong, Lin Song, Changxi Bai, Buhechaolu Wang, Chula Sa","doi":"10.1155/2024/3525212","DOIUrl":"https://doi.org/10.1155/2024/3525212","url":null,"abstract":"<i>Leontopodium leontopodioides</i> (Willd.) Beauv. (<i>L. leontopodioides</i>.) has been used to treat lung diseases in traditional Chinese medicine (TCM). However, a systematic analysis of its chemical components has not been reported so far. In this study, UPLC-Q-Orbitrap MS and GC-MS were applied to investigate the chemical composition of the water extracts and essential oils of <i>L. leontopodioides.</i> UPLC-Q-Orbitrap MS adopts a heating electrospray ionization source, collecting primary and secondary mass spectrometry data in positive and negative ions, respectively, and uses Compound Discoverer 3.2 software to analyze the collected raw data. As a result, a total of 39 compounds were identified from their high-resolution mass spectra in both positive and negative ionization modes, including 13 flavonoids and their glycosides, 15 phenolic acids, 4 oligosaccharides and glycosides, 4 pentacyclic triterpenoids, and 3 other compounds. Among them, 18 chemical components have not been reported in <i>L. leontopodioides</i>. In the GC-MS section, two common organic solvents (n-hexane and diethyl ether) were used to extract essential oils, and the mass spectra were recorded at 70 eV (electron impact) and scanned in the range of 35∼450 m/z. Compounds were identified using NIST (version 2017), and the peak area normalization method was used to calculate their relative amounts. Finally, 17 components were identified in the volatile oil extracted with n-hexane, accounting for 80.38% of the total volatile oil, including monoterpenoids, phenylpropene, fatty acids, and aliphatic hydrocarbons. In the volatile oil extracted with diethyl ether, 16 components were identified, accounting for 73.50% of the total volatile oil, including phenylpropene, aliphatic hydrocarbons, monoterpenoids, fatty acids, and esters. This study was the first to conduct a comprehensive analysis of the chemical composition of the <i>L. leontopodioides</i> water extract and its essential oil, and a comprehensive chemical composition spectrum was constructed, to lay a foundation for its further pharmacodynamic material basis and quality evaluation.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140883416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting of Green Synthesized Metal Oxide Nanoparticles in the Potentiometric Determination of Metformin Hydrochloride in Pharmaceutical Products","authors":"Shikhah Almutairi, Nawal A. Alarfaj, Adibah M. Almutairi, Maha F. El-Tohamy","doi":"10.1155/2024/8354311","DOIUrl":"https://doi.org/10.1155/2024/8354311","url":null,"abstract":"The advanced and highly functional properties of Al₂O₃ and NiO nanoparticles promote the widespread use of metal oxides as remarkable electroactive materials for sensing and electrochemical applications. The proposed study describes a comparison of the sensitivity and selectivity of two modified wire membrane sensors enriched with Al₂O₃ and NiO nanoparticles with conventional wire membranes for the quantification of the antidiabetic drug metformin hydrochloride (MTF). The results show linear relationships of the enriched Al₂O₃ and NiO nanosensors over the concentration ranges 1.0 × 10⁻¹⁰–1.0 × 10⁻² mol L⁻¹ and 1.0 × 10⁻⁶–1.0 × 10⁻² M for both the modified sensors and the conventional coated wire membrane sensors. The regression equations were <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"></path></g></svg> = (52.1 ± 0.5) log (MTF) + 729 for enriched nanometallic oxides, <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-70\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"><use xlink:href=\"#g190-110\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"><use xlink:href=\"#g190-87\"></use></g></svg> = (57.04 ± 0.4) log (MTF) + 890.66, and <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-70\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"><use xlink:href=\"#g190-110\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"><use xlink:href=\"#g190-87\"></use></g></svg> = (58.27 ± 0.7) log (MTF) + 843.27 with correlation coefficients of 0.9991, 0.9997, and 0.9998 for the aforementioned sensors, respectively. The proposed method was fully validated with respect to the recommendations of the International Union of Pure and Applied Chemistry (IUPAC). The newly functionalized sensors have been successfully used for the determination of MTF in its commercial products.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"121 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine Test Strip Quantitative Assay with a Smartphone Camera","authors":"Miroslav Pohanka, Jitka Zakova","doi":"10.1155/2024/6004970","DOIUrl":"https://doi.org/10.1155/2024/6004970","url":null,"abstract":"Urine test strips for urinalysis are a common diagnostic tool with minimal costs and are used in various situations including homecare and hospitalization. The coloration scaled by the naked eye is simple, but it is suitable for semiquantitative analysis only. In this paper, a colorimetric assay is developed based on a smartphone digital camera and urine test strips. Assays of pH, albumin, glucose, and lipase activity were performed as a tool for the diagnosis of aciduria, alkaluria, glycosuria, proteinuria, and leukocyturia. The RGB color channels were analyzed in the colorimetric assay, and the assay exerted good sensitivity, and all the particular diagnoses proved to be reliable. The limits of detection for glucose (0.11 mmol/L), albumin (0.15 g/L), and lipase (2.50 U/<i>μ</i>L) were low enough to cover the expected physiological concentration, and the range for pH was also satisfactory. The urine test strips with a camera as an output detector proved applicability to spiked urine samples, and the results were also well in comparison to the standard assays which confirms the practical relevance of the presented findings.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2012 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140147221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simple and Rapid LC-MS/MS Method for the Quantification of Nirmatrelvir/Ritonavir in Plasma of Patients with COVID-19","authors":"Xiujing Zhu, Lin Li, Bin Dai, Zhijun Liu, Zhipeng Wang, Lili Cui, Shouhong Gao, Wansheng Chen, Xia Tao, Deduo Xu","doi":"10.1155/2024/6139928","DOIUrl":"https://doi.org/10.1155/2024/6139928","url":null,"abstract":"The combined prescriptions of nirmatrelvir/ritonavir and other drugs are limited due to potential drug-drug interactions, so therapeutic drug monitoring (TDM) becomes particularly important. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for determination of the nirmatrelvir/ritonavir in plasma of patients with COVID-19, providing technical and theoretical support for the TDM. Plasma samples were processed by protein precipitation using acetonitrile, and analytes were separated on an Agilent Poroshell 120 SB-C18 (2.1 × 75 mm, 2.7 <i>μ</i>m) column at 35°C. Acetonitrile and 0.1% formic acid in water (52 : 48) were utilized as the mobile phases at a flow rate of 0.3 mL/min. In the multiple reaction monitoring (MRM) mode, nirmatrelvir and ritonavir were monitored using precursor/product ions: <i>m</i>/<i>z</i> 500.2/110.1 and 721.3/296.1, respectively, with selinexor as the internal standard. The linear range of both analytes was 2.0 ng/mL to 5000 ng/mL with good inter- and intraday precision and accuracy, and the recovery was 92.0%–107% for nirmatrelvir and 85.7%–106% for ritonavir. Finally, this method was successfully applied to monitor the exposure levels of nirmatrelvir/ritonavir in plasma samples from hemodialysis patients.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"48 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS","authors":"Xiuwei Shen, Ziyue Wang, Wenting Li, Shenshen Mei, Shunjun Ma, Xianqin Wang, Congcong Wen, Fan Chen, Guojun Zheng","doi":"10.1155/2024/7971021","DOIUrl":"https://doi.org/10.1155/2024/7971021","url":null,"abstract":"Ziyuglycoside I and ziyuglycoside II are important active components of <i>Sanguisorba officinalis</i> L., which have excellent pharmacological effects, such as antioxidant and anticancer effects. However, the bioavailability of ziyuglycoside I and ziyuglycoside II has not been reported. This work aims to establish a UPLC-MS/MS method to study the pharmacokinetics of ziyuglycoside I and ziyuglycoside II in rats under different administration routes (intragastric and intravenous administration) and to calculate the bioavailability. The concentration of ziyuglycoside I and ziyuglycoside II in rat plasma in the range of 2–2000 ng/mL showed a good linear relationship (<i>r</i> &gt; 0.99). The intra-day accuracies of ziyuglycoside I and ziyuglycoside II ranged from 87% to 110%, and the inter-day accuracies ranged from 97% to 109%. The intra-day precision was less than 15% and the inter-day precision was less than 14%. The matrix effects ranged from 88% to 113%. The recoveries were all above 84%. The developed UPLC-MS/MS method for the determination of ziyuglycoside I and ziyuglycoside II in rat plasma was applied to pharmacokinetics. The bioavailability of ziyuglycoside I and ziyuglycoside II was measured at 2.6% and 4.6%, respectively.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"167 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140006712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus","authors":"Liang Ma, Haoyan Zhu, Yongwei Jiang, Xiaomu Kong, Peng Gao, Yi Liu, Meimei Zhao, Guoxiong Deng, Yongtong Cao","doi":"10.1155/2024/4950391","DOIUrl":"https://doi.org/10.1155/2024/4950391","url":null,"abstract":"<i>Objective</i>. A sensitive and specific multiplex fluorescence rapid detection method was established for simultaneous detection of SARS-CoV-2, influenza A virus, and influenza B virus in a self-made device within 30 min, with a minimum detection limit of 200 copies/mL. <i>Methods</i>. Based on the genome sequences of SARS-CoV-2, influenza A virus (FluA), and influenza B virus (FluB) with reference to the Chinese Center for Disease Control and Prevention and related literature, specific primers were designed, and a multiplex fluorescent PCR system was established. The simultaneous and rapid detection of SARS-CoV-2, FluA, and FluB was achieved by optimizing the concentrations of Taq DNA polymerase as well as primers, probes, and Mg²⁺. The minimum detection limits of the nucleic acid rapid detection system for SARS-CoV-2, FluA, and FluB were evaluated. <i>Results</i>. By optimizing the amplification system, the N enzyme with the best amplification performance was selected, and the optimal concentration of Mg²⁺ in the multiamplification system was 3 mmol/L; the final concentrations of SARS-CoV-2 NP probe and primer were 0.15 <i>μ</i>mol/L and 0.2 <i>μ</i>mol/L, respectively; the final concentrations of SARS-CoV-2 ORF probe and primer were both 0.15 <i>μ</i>mol/L; the final concentrations of FluA probe and primer were 0.2 <i>μ</i>mol/L and 0.3 <i>μ</i>mol/L, respectively; the final concentrations of FluB probe and primer were 0.15 <i>μ</i>mol/L and 0.25 <i>μ</i>mol/L, respectively. <i>Conclusion</i>. A multiplex real-time quantitative fluorescence RT-PCR system for three respiratory viruses of SARS-CoV-2, FluA, and FluB was established with a high amplification efficiency and sensitivity reaching 200 copies/mL for all samples. Combined with the automated microfluidic nucleic acid detection system, the system can achieve rapid detection in 30 minutes.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"250 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140007010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation and Application of Screen-Printed Microchip for Potentiometric Determination of Metformin Hydrochloride in Tablet Dosage Form","authors":"Mohammed Alqarni, Abdullah A. Alshehri, Hassan Arida","doi":"10.1155/2024/8664723","DOIUrl":"https://doi.org/10.1155/2024/8664723","url":null,"abstract":"Metformin is an oral biguanides hypoglycaemic agent, which used to lower the blood glucose levels in people with type 2 diabetes mellitus. Many analytical techniques have been used to quantify the drug in different pharmaceutical dosage forms; however, most of these methods have limited throughput in the quality control application. A disposable potentiometric microsensor responsive to metformin has recently been reported. For the first time, herein, this method of analysis has been validated according to IUPAC recommendations and successfully applied in the determination of metformin drug in some dosage form. Different drug formulations of metformin hydrochloride have been collected from the local pharmaceutical stores in Saudi Arabia and analysed using the validated microchip-based method of analysis. Subsequently, the results of this study showed that the validated method was linear, specific, precise, and accurate. The linear range was 1 × 10⁻¹–1 × 10⁻⁵ mol L⁻¹ and the correlation coefficient was 0.999. The limit of detection was 2.89 × 10⁻⁶ mol L⁻¹, and the limit of quantification was 8.77 × 10⁻⁶ mol L⁻¹. This method demonstrated high precision, with an RSD% of less than 2.22%. The accuracy of this method was obtained by comparing the recovery percentage with percentage values less than 5%. The results obtained showed that there was no significant difference between the references, label, and recovery of less than 5%.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"79 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139978283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}