International Journal of Analytical Chemistry最新文献

{"title":"Green and High Throughput Assay Using 96-Microwell Base to Determine Metformin Hydrochloride in the Tablet Dosage Form.","authors":"Mohammed Alqarni, Atheer Alshehri, Bayan Almalki, Refah Althumali, Maram Alghamdi, Rawan Alqahtani, Safia G Alotibi, Ali Alqarni, Adel H Awad, Ibrahim A Naguib","doi":"10.1155/2024/3374034","DOIUrl":"https://doi.org/10.1155/2024/3374034","url":null,"abstract":"<p><p>Metformin (MET) is an oral antidiabetic drug widely used as the primary treatment for type 2 diabetes mellitus (T2DM). While various spectrophotometric assays exist for determining MET in pharmaceutical formulations, they often have limited throughput for quality control purposes. This study describes the validation of a 96-microwell plate spectrophotometer method using charge-transfer complexes (CTCs) with chloranilic acid (CLA) and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) for the quality control and detected of MET. This reaction was carried out in 96-microwell plates, and the absorbance of the colored complexes of CLA and DDQ were measured at 530 nm and 460 nm, respectively, using an absorbance microplate reader. This study aims to identify and quantify the use of a 96-microwell plate spectrophotometer analytical technique for assessing complicated formulations. The method was successfully used for the quantification of MET in the tablet dosage form. The results showed good correlation coefficients (0.996 and 0.997) with CLA and DDQ, respectively. The present method showed high precision with RSD % not exceeding 2.17%. The accuracy of the method was obtained by recovery percentage, with percentage values less than ±5%. The Analytical Greenness Metric (AGREE) was used to evaluate greenness of the assays. The result show that the microwell assay method is greenness and suitable for handling large samples on a daily used with high throughput analysis. The use of the 96-microwell-plate method is superior to the existing method in terms of simplicity of the procedure, the low economic cost, and its consumption of low amounts of reagents and organic ethanol solvent, making it an environmentally friendly method. Therefore, these advantages make them suitable and rapid alternatives method to current methods for routine metformin analysis in quality control laboratories.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2024 ","pages":"3374034"},"PeriodicalIF":1.5,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Effective Sol-Gel-Functionalized Polyurethane Foams Solid Platform Packed Minicolumns for Complete Extraction of Chromium (VI) from Water: Kinetic, Sorption Isotherms, Thermodynamic Study, and Analytical Utility.","authors":"Effat A Bahaidarah","doi":"10.1155/2024/3152894","DOIUrl":"https://doi.org/10.1155/2024/3152894","url":null,"abstract":"<p><p>In the modern era, sol-gel plays a key role in the progress of a new generation of dispersive solid-phase microextractors (d-<i>µ</i> SPMEs) for the removal of organic and inorganic pollutants in complex matrices. Thus, the current study reports the use of sol-gel-functionalized polyurethane foams (PUFs) as a novel solid platform for complete extraction of chromium (VI) species from aqueous media. The planned protocol was based upon the complete extraction of the formed binary complex ion associates between the protonated ether and/or urethane groups of PUFs and chlorochromate anion [CrO₃Cl]^- _aq in aqueous HCl (≥1M) medium in addition to H-bonding and the electrostatic <i>π</i>-<i>π</i> interaction that resulted between the CrO₃Cl^- and the silanol group (Si/ZrO₂, Si-O-Zr) and siloxane (Si-O-Si) groups of the sol-gel. The impact of the analytical parameters (solution pH, natural mineral acids, shaking time, temperature, and chromium (VI) concentrations) was critically studied. At the optimal conditions, the uptake capacity of the established extractor (9.9 mg·g^-1) was in agreement with the Langmuir adsorption capacity (12.08 mg·g^-1) of the monolayer. The sorption data fitted well with the pseudo first-order kinetic model (<i>R</i> ² = 0.9961) with an overall rate constant (<i>k</i> ₁) of 0.081 min^-1 and an equilibrium capacity (<i>q</i> _<i>e</i> ) of 8.6 mg·g^-1, which is in a good agreement with the experimental value (9.9 mg·g^-1). The sorption of the oxyion [CrO₃Cl]^- _aq onto the solid sorbent is an endothermic and spontaneous process as reflected from the values of ΔH (6.99 kJ·mol^-1) and Δ<i>G</i> (-8.14 kJ·mol^-1 at 293 K), respectively. The Δ<i>S</i> value (15.13 kJ·mol^-1·K^-1) reflects that the [CrO₃Cl]^- _aq retention onto the sol-gel-treated PUFs sorbent proceeded in a more unplanned fashion. Sol-gel-treated PUFs sorbent-packed minicolumns were successfully used for the complete removal of trace levels of chromium (VI) species from water samples. Sorbed chromium (VI) species were recovered with NaOH (0.5 M) and analysed by spectrophotometry, which supports the utility of the sol-gel-treated PUFs as a low-cost solid extractor for water treatment.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2024 ","pages":"3152894"},"PeriodicalIF":1.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Rhodojaponin II and Rhodojaponin III in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.","authors":"Cheng Sun, Wanhang Wang, Xi Bao, Dizhong Chen, Shenshen Mei, Jianshe Ma, Xiajuan Jiang","doi":"10.1155/2024/6386570","DOIUrl":"10.1155/2024/6386570","url":null,"abstract":"<p><p>An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to determine the concentrations of Rhodojaponin II and Rhodojaponin III in rat plasma, and their pharmacokinetic profiles were investigated. A UPLC HSS T3 (2.1 mm × 50 mm, 1.8 <i>μ</i>m) chromatographic column was employed at a temperature of 40°C. The mobile phase consisted of acetonitrile-0.1% formic acid in water, and a gradient elution method with an elution time of 6 min and flow rate of 0.4 mL/min was utilized for analysis purposes. Methodological investigations were conducted accordingly. The plasma concentrations of Rhodojaponin II and Rhodojaponin III exhibited excellent linearity within the range of 2 ng/mL-1250 ng/mL. Moreover, both intraday and interday precision were below 15%, while accuracy ranged from 88% to 115%. Additionally, matrix effect fell within the range of 90%-110%, and recoveries ranged from 78% to 87%. These results comply with relevant regulations for drug analysis in biological samples. Therefore, this method is deemed suitable for quantifying Rhodojaponin II and Rhodojaponin III levels in rats.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2024 ","pages":"6386570"},"PeriodicalIF":1.5,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residents' Knowledge regarding Recreational Drug Screening Immunoassays at a Swiss Hospital Group.","authors":"Elias Bekka, Samuel E Christen, Laura Hermann, Aristomenis K Exadaktylos, Manuel Haschke, Evangelia Liakoni","doi":"10.1155/2024/4400606","DOIUrl":"10.1155/2024/4400606","url":null,"abstract":"<p><strong>Introduction: </strong>In case of suspected acute recreational drug toxicity, immunoassays are commonly used as diagnostic tools. Although easy to handle, understanding of their limitations is necessary for a correct interpretation of the results. The aim of this project was to investigate residents' knowledge regarding drug screening immunoassays at a Swiss hospital group.</p><p><strong>Methods: </strong>All residents of a large hospital group in Switzerland were invited by e-mail to participate in an anonymous survey. Following ten multiple choice questions on drug screening tests, the participants were also asked about their demographics, whether they used drug screening tests on a regular basis, and how confident they felt in their ability to interpret test results.</p><p><strong>Results: </strong>The ten knowledge questions were answered by 110 of the 1026 residents (11%). Among the 108 participants with available demographics, 90% were 25-35 years old, 63% were female, and 70% were at least in their 4^th year of residency. The median score of correct answers was 4 out of 10 (range 0-7) and in 50% of the questions, the correct answer was the most frequently selected response. No significant differences in the knowledge scores were found based on the training, confidence level, or the frequency of drug tests used in daily work.</p><p><strong>Conclusion: </strong>This survey revealed widespread knowledge gaps among residents regarding the interpretation of immunoassay-based drug test results. These findings can be used to implement educational measures on this topic and might provide a basis for targeted information on common pitfalls to be included in laboratory reports.</p>","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2024 ","pages":"4400606"},"PeriodicalIF":1.5,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure Elucidation of a Novel Polysaccharide Isolated from Euonymus fortunei and Establishing Its Antioxidant and Anticancer Properties","authors":"Yu Luo, Hongtao Chen, Chunxi Huang, Shujia He, Qilong Wen, Danzhao Cai","doi":"10.1155/2024/8871600","DOIUrl":"https://doi.org/10.1155/2024/8871600","url":null,"abstract":"<i>Euonymus</i><i>fortunei</i> polysaccharides (EFPs) have not been extensively investigated yet in terms of their extraction and biological activity. The orthogonal experimental design was employed in this study to evaluate the optimum yield of EFPs. A maximum yield of 2.63 ± 0.23% was attained using material-liquid ratios of 60 mL/g, extraction temperature of 80°C, ultrasonic power of 144 W, and extraction time of 75 mins. The polysaccharide content reached 53.47 ± 0.31% when deproteinized thrice. An analysis of monosaccharide composition revealed that these polysaccharides consist of Gal, Glc, Man, Fuc, and Rha with a molar ratio of 7.14 ∶ 23.99 ∶ 6.29 ∶ 6.55 ∶ 1.00, respectively, in EFPs. Subsequently, the <i>in vitro</i> scavenging capacities of 2,2-diphenylpicrylhydrazyl (DPPH) and ·OH and superoxide anion radicals, along with the reducing power of EFPs, were studied. Results revealed that EFPs have higher antioxidant activity, particularly ·OH scavenging, as well as reducing power, as compared to <i>Astragalus</i> polysaccharides (ASPs) and <i>Lycium barbarum</i> polysaccharides (LBPs). The Cell Counting Kit-8 (CCK-8) method was used to evaluate the effects of different concentrations of polysaccharides on SKOV3 cell proliferation, and the results revealed their inhibition at concentrations in the range of 200–800 <i>μ</i>g/mL. In addition, findings from flow cytometry further confirmed that EFPs blocked the cell cycle at G0/G1 and S phases and induced SKOV3 cell apoptosis. In a word, EFPs could be exploited and used further based on the experimental results from this study.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"9 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141147546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorption Characteristics and Chromatographic Separation of 90Y3+ from 90Sr2+ from Aqueous Media by Chelex-100 (Anion Ion Exchange) Packed Column","authors":"Mohammad S. El-Shahawi, Hassan Alwael, Abdulaziz A. Alsibaai, Abdelgany Hamza, Faisal K. Algethami, Fatmah M. Alshareef, Sanaa H. El-Khouly, Neven Eweda","doi":"10.1155/2024/6232381","DOIUrl":"https://doi.org/10.1155/2024/6232381","url":null,"abstract":"There is growing demand for separation of ⁹⁰Y carrier free from ⁹⁰Sr coexisting to produce high purity ⁹⁰Y essential for radiopharmaceutical uses. Thus, in this context the sorption profiles of Y³⁺ and Sr²⁺ from aqueous solutions containing diethylenetriaminepenta acetic acid (DTPA), ethylenediaminetetra-acetic acid (EDTA), acetic acid, citric acid, or NaCl onto Chelex-100 (anion ion exchange) solid sorbent were critically studied for developing an efficient and low-cost methodology for selective separation of Y³⁺ from Sr²⁺ ions (1.0 × 10⁻⁵ M). Batch experiments displayed relative chemical extraction percentage (98 ± 5.4%) of Y³⁺ from aqueous acetic acid solution onto Chelex-100 (anion ion exchanger), whereas Sr²⁺ species showed no sorption. Hence, a selective separation of Y³⁺ from its parent ⁹⁰Sr²⁺ has been established based upon percolation of the aqueous solution of Y³⁺ and Sr²⁺ ions containing acetic acid at pH 1-2 through Chelex-100 sorbent packed column at a 2 mL min⁻¹ flow rate. Y³⁺ species were retained quantitatively while Sr²⁺ ions were not sorbed and passed through the sorbent packed column without extraction. The sorbed Y³⁺ species were then recovered from the sorbent packed column with HNO₃ (1.0 M) at a 1.0 mL min⁻¹ flow rate. A dual extraction mechanism comprising absorption associated to “weak-base anion exchanger” and “solvent extraction” of Y³⁺ as (YCl₆)³⁻ and an extra part for “surface adsorption” of Y³⁺ by the sorbent is proposed. The established method was validated by measuring the radiochemical (99.2 ± 2 1%), radionuclide purity and retardation factor (<svg height=\"14.1649pt\" style=\"vertical-align:-5.529pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 14.5964 14.1649\" width=\"14.5964pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,8.086,3.132)\"></path></g></svg> = 10.0 ± 0.1 cm) of ⁹⁰Y³⁺ recovered in the eluate. Ultimately, the sorbent packed column also presented high stability for reusing 2-3 cycles without drop in its efficiency (±5%) towards Y³⁺ uptake and relative chemical recovery. A proposed flow sheet describing the analytical procedures for the separation of ⁹⁰Y³⁺ from ⁹⁰Sr²⁺ using chelating Chelex 100 (anion exchange) packed column is also included.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"64 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic Properties of Baitouweng Decoction in Bama Miniature Pigs: Implications for Clinical Application in Humans","authors":"Qianqian Xu, Huilan Gao, Fuqiang Zhu, Wenliang Xu, Yubo Wang, Jinwen Xie, Guangjun Guo, Limei Yang, Li Ma, Zhiqiang Shen, Jichang Li","doi":"10.1155/2024/5535752","DOIUrl":"https://doi.org/10.1155/2024/5535752","url":null,"abstract":"Traditional Chinese medicine (TCM) serves as a significant adjunct to chemical treatment for chronic diseases. For instance, the administration of Baitouweng decoction (BTWD) has proven effective in the treatment of ulcerative colitis. However, the limited understanding of its pharmacokinetics (PK) has impeded its widespread use. Chinese Bama miniature pigs possess anatomical and physiological similarities to the human body, making them a valuable model for investigating PK properties. Consequently, the identification of PK properties in Bama miniature pigs can provide valuable insights for guiding the clinical application of BTWD in humans. To facilitate this research, a rapid and sensitive UPLC-MS/MS method has been developed for the simultaneous quantification of eleven active ingredients of BTWD in plasma. Chromatographic separation was conducted using an Acquity UPLC HSS T3 C₁₈ column and a gradient mobile phase comprising acetonitrile and water (containing 0.1% acetic acid). The methodology was validated in accordance with the FDA Bioanalytical Method Validation Guidance for Industry. The lower limit of quantitation fell within the range of 0.60–2.01 ng/mL. Pharmacokinetic studies indicated that coptisine chloride, berberine, columbamine, phellodendrine, and obacunone exhibited low <span><svg height=\"11.9316pt\" style=\"vertical-align:-3.2957pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 25.3434 11.9316\" width=\"25.3434pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,8.619,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,16.181,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,20.267,3.132)\"></path></g></svg>,</span> while fraxetin, esculin, fraxin, and pulchinenoside <svg height=\"11.5695pt\" style=\"vertical-align:-3.18152pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.38798 12.7187 11.5695\" width=\"12.7187pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,7.644,3.132)\"></path></g></svg> were rapidly absorbed and eliminated from the plasma. These findings have implications for the development of effective components in BTWD and the adjustment of clinical dosage regimens.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"124 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140925966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Analysis of Leontopodium leontopodioides (Willd.) Beauv. Chemical Composition by GC/MS and UPLC-Q-Orbitrap MS","authors":"Yuanyuan Chen, Yu Dong, Lin Song, Changxi Bai, Buhechaolu Wang, Chula Sa","doi":"10.1155/2024/3525212","DOIUrl":"https://doi.org/10.1155/2024/3525212","url":null,"abstract":"<i>Leontopodium leontopodioides</i> (Willd.) Beauv. (<i>L. leontopodioides</i>.) has been used to treat lung diseases in traditional Chinese medicine (TCM). However, a systematic analysis of its chemical components has not been reported so far. In this study, UPLC-Q-Orbitrap MS and GC-MS were applied to investigate the chemical composition of the water extracts and essential oils of <i>L. leontopodioides.</i> UPLC-Q-Orbitrap MS adopts a heating electrospray ionization source, collecting primary and secondary mass spectrometry data in positive and negative ions, respectively, and uses Compound Discoverer 3.2 software to analyze the collected raw data. As a result, a total of 39 compounds were identified from their high-resolution mass spectra in both positive and negative ionization modes, including 13 flavonoids and their glycosides, 15 phenolic acids, 4 oligosaccharides and glycosides, 4 pentacyclic triterpenoids, and 3 other compounds. Among them, 18 chemical components have not been reported in <i>L. leontopodioides</i>. In the GC-MS section, two common organic solvents (n-hexane and diethyl ether) were used to extract essential oils, and the mass spectra were recorded at 70 eV (electron impact) and scanned in the range of 35∼450 m/z. Compounds were identified using NIST (version 2017), and the peak area normalization method was used to calculate their relative amounts. Finally, 17 components were identified in the volatile oil extracted with n-hexane, accounting for 80.38% of the total volatile oil, including monoterpenoids, phenylpropene, fatty acids, and aliphatic hydrocarbons. In the volatile oil extracted with diethyl ether, 16 components were identified, accounting for 73.50% of the total volatile oil, including phenylpropene, aliphatic hydrocarbons, monoterpenoids, fatty acids, and esters. This study was the first to conduct a comprehensive analysis of the chemical composition of the <i>L. leontopodioides</i> water extract and its essential oil, and a comprehensive chemical composition spectrum was constructed, to lay a foundation for its further pharmacodynamic material basis and quality evaluation.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"15 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140883416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting of Green Synthesized Metal Oxide Nanoparticles in the Potentiometric Determination of Metformin Hydrochloride in Pharmaceutical Products","authors":"Shikhah Almutairi, Nawal A. Alarfaj, Adibah M. Almutairi, Maha F. El-Tohamy","doi":"10.1155/2024/8354311","DOIUrl":"https://doi.org/10.1155/2024/8354311","url":null,"abstract":"The advanced and highly functional properties of Al₂O₃ and NiO nanoparticles promote the widespread use of metal oxides as remarkable electroactive materials for sensing and electrochemical applications. The proposed study describes a comparison of the sensitivity and selectivity of two modified wire membrane sensors enriched with Al₂O₃ and NiO nanoparticles with conventional wire membranes for the quantification of the antidiabetic drug metformin hydrochloride (MTF). The results show linear relationships of the enriched Al₂O₃ and NiO nanosensors over the concentration ranges 1.0 × 10⁻¹⁰–1.0 × 10⁻² mol L⁻¹ and 1.0 × 10⁻⁶–1.0 × 10⁻² M for both the modified sensors and the conventional coated wire membrane sensors. The regression equations were <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"></path></g></svg> = (52.1 ± 0.5) log (MTF) + 729 for enriched nanometallic oxides, <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-70\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"><use xlink:href=\"#g190-110\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"><use xlink:href=\"#g190-87\"></use></g></svg> = (57.04 ± 0.4) log (MTF) + 890.66, and <svg height=\"11.9087pt\" style=\"vertical-align:-3.2728pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 21.614 11.9087\" width=\"21.614pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-70\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,7.943,3.132)\"><use xlink:href=\"#g190-110\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,14.537,3.132)\"><use xlink:href=\"#g190-87\"></use></g></svg> = (58.27 ± 0.7) log (MTF) + 843.27 with correlation coefficients of 0.9991, 0.9997, and 0.9998 for the aforementioned sensors, respectively. The proposed method was fully validated with respect to the recommendations of the International Union of Pure and Applied Chemistry (IUPAC). The newly functionalized sensors have been successfully used for the determination of MTF in its commercial products.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"121 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140597162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine Test Strip Quantitative Assay with a Smartphone Camera","authors":"Miroslav Pohanka, Jitka Zakova","doi":"10.1155/2024/6004970","DOIUrl":"https://doi.org/10.1155/2024/6004970","url":null,"abstract":"Urine test strips for urinalysis are a common diagnostic tool with minimal costs and are used in various situations including homecare and hospitalization. The coloration scaled by the naked eye is simple, but it is suitable for semiquantitative analysis only. In this paper, a colorimetric assay is developed based on a smartphone digital camera and urine test strips. Assays of pH, albumin, glucose, and lipase activity were performed as a tool for the diagnosis of aciduria, alkaluria, glycosuria, proteinuria, and leukocyturia. The RGB color channels were analyzed in the colorimetric assay, and the assay exerted good sensitivity, and all the particular diagnoses proved to be reliable. The limits of detection for glucose (0.11 mmol/L), albumin (0.15 g/L), and lipase (2.50 U/<i>μ</i>L) were low enough to cover the expected physiological concentration, and the range for pH was also satisfactory. The urine test strips with a camera as an output detector proved applicability to spiked urine samples, and the results were also well in comparison to the standard assays which confirms the practical relevance of the presented findings.","PeriodicalId":13888,"journal":{"name":"International Journal of Analytical Chemistry","volume":"2012 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140147221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}