International Journal of Biomedical Science : IJBS最新文献_第8页

Lack of Association between Mannose-binding Lectin 2 Codons 54 and 57 Gene Polymorphisms and Cervicovaginal Infections in Mexican Women. 甘露糖结合凝集素2密码子54和57基因多态性与墨西哥妇女宫颈阴道感染缺乏相关性

International Journal of Biomedical Science : IJBS Pub Date : 2017-06-01

Nadia Velazquez-Hernandez, Marisela Aguilar-Duran, Alma Rosa Perez-Alamos, Sergio Estrada-Martinez, Jose M Salas-Pacheco, Luis Francisco Sanchez-Anguiano, Ada A Sandoval-Carrillo, Brissia Lazalde-Medina, Cosme Alvarado-Esquivel

{"title":"Lack of Association between Mannose-binding Lectin 2 Codons 54 and 57 Gene Polymorphisms and Cervicovaginal Infections in Mexican Women.","authors":"Nadia Velazquez-Hernandez, Marisela Aguilar-Duran, Alma Rosa Perez-Alamos, Sergio Estrada-Martinez, Jose M Salas-Pacheco, Luis Francisco Sanchez-Anguiano, Ada A Sandoval-Carrillo, Brissia Lazalde-Medina, Cosme Alvarado-Esquivel","doi":"","DOIUrl":"","url":null,"abstract":"The mannose-binding lectin (MBL) 2 gene has an important function in the innate immune response and activation of the third pathway of the complement system. Some studies have assessed the association of the MBL2 gene polymorphisms with cervicovaginal infections (CVI); however, there is no information about this association in Mexican women. This study aimed to determine the association between the MBL2 codons 54 and 57 gene polymorphisms with CVI in a sample of Mexican women. Through a cross-sectional study, blood samples and cervicovaginal cultures were obtain from 354 women. MBL2 genotyping was performed by real-time polymerase chain reaction with Taqman probes. Of the 354 women studied, 128 (36.2%) had CVI and 226 (63.8%) were healthy. The frequencies of the C and T variants in codon 54 in women with CVI were 83% and 17%, respectively; whereas the frequencies of these variants in healthy women were 82% and 18%, respectively. The frequencies of variants C/C, C/T, and T/T in women with CVI were 68%, 31%, and 1%, respectively; whereas the frequencies of these variants in healthy women were 68%, 29%, and 3%, respectively. With respect to codon 57, the frequencies of variants C and T were identical in women with CVI and in healthy women (97% and 3%, respectively). The frequencies of variants C/C, C/T, and T/T were identical in women with CVI and in healthy women (94%, 6%, and 0%, respectively). We conclude that MBL2 codons 54 and 57 gene polymorphisms do not associate with CVI in Mexican women.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 2","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35284498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forensic Odontology: The New Dimension in Dental Analysis 法医牙科学:牙齿分析的新维度

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01 DOI: 10.59566/ijbs.2017.13001

K. Divakar

引用次数: 42

Effect of Loss of Teeth and its Association with General Quality of Life using Geriatric Oral Health Assessment Index (Gohai) among Older Individuals Residing in Rural Areas 用老年口腔健康评估指数(Gohai)分析农村老年人牙齿脱落的影响及其与总体生活质量的关系

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01 DOI: 10.59566/ijbs.2017.13006

Vinaya Kundapur, R. Hegde, M. Shetty, S. Mankar, M. Hilal, Hari Prasad A

{"title":"Effect of Loss of Teeth and its Association with General Quality of Life using Geriatric Oral Health Assessment Index (Gohai) among Older Individuals Residing in Rural Areas","authors":"Vinaya Kundapur, R. Hegde, M. Shetty, S. Mankar, M. Hilal, Hari Prasad A","doi":"10.59566/ijbs.2017.13006","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13006","url":null,"abstract":"The development of measures for assessing oral health status is essential to the evolution and maturation of a scientific knowledge based in geriatric dentistry. Their development as branches of clinical care depends in part as ability to demonstrate an effective grasp of the problems of elderly and their solutions. Any strategy for altering the health status of elderly requires a technology for first assessing that health status and then detecting increments of progress. Development of geriatric oral health assessment index (GOHAI) is a self reported measure designed to assess the oral health problems of older individuals. The goal of geriatric assessment is to collect information that will facilitate diagnosis and suggest appropriate interventions. Such a measure would facilitate estimation of prevalence of oral functional problems in older individuals, It would also increase understanding of psychological impact of oral disease and provide a means for comparing the effectiveness of various dental treatment. It also emphasizes on social well being and reflects quality of life following replacement of missing teeth.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"111 1","pages":"6 - 12"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79290454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of Loss of Teeth and its Association with General Quality of Life using Geriatric Oral Health Assessment Index (Gohai) among Older Individuals Residing in Rural Areas. 使用老年口腔健康评估指数（Gohai）评估居住在农村地区的老年人牙齿脱落的影响及其与总体生活质量的关系。

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01

Vinaya Kundapur, Rakshith Hegde, Manoj Shetty, Sunil Mankar, Mohammed Hilal, Hari Prasad A

{"title":"Effect of Loss of Teeth and its Association with General Quality of Life using Geriatric Oral Health Assessment Index (Gohai) among Older Individuals Residing in Rural Areas.","authors":"Vinaya Kundapur, Rakshith Hegde, Manoj Shetty, Sunil Mankar, Mohammed Hilal, Hari Prasad A","doi":"","DOIUrl":"","url":null,"abstract":"The development of measures for assessing oral health status is essential to the evolution and maturation of a scientific knowledge based in geriatric dentistry. Their development as branches of clinical care depends in part as ability to demonstrate an effective grasp of the problems of elderly and their solutions. Any strategy for altering the health status of elderly requires a technology for first assessing that health status and then detecting increments of progress. Development of geriatric oral health assessment index (GOHAI) is a self reported measure designed to assess the oral health problems of older individuals. The goal of geriatric assessment is to collect information that will facilitate diagnosis and suggest appropriate interventions. Such a measure would facilitate estimation of prevalence of oral functional problems in older individuals, It would also increase understanding of psychological impact of oral disease and provide a means for comparing the effectiveness of various dental treatment. It also emphasizes on social well being and reflects quality of life following replacement of missing teeth.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"6-12"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35018516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tobacco Smoke Exposure Reduces Paraoxonase Activity in a Murine Model. 烟草烟雾暴露降低小鼠模型中的对氧磷酶活性。

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01

Robert M Reed, Saif M Borgan, Michael Eberlein, Monica Goldklang, Joshua Lewis, Michael Miller, Mohamad Navab, Bo S Kim

{"title":"Tobacco Smoke Exposure Reduces Paraoxonase Activity in a Murine Model.","authors":"Robert M Reed, Saif M Borgan, Michael Eberlein, Monica Goldklang, Joshua Lewis, Michael Miller, Mohamad Navab, Bo S Kim","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To demonstrate a direct inhibitory effect of cigarette smoke exposure on paraoxonase 1 activity in a murine in vivo model.Methods: At 8 weeks old, we randomized 10 C57/bl6 mice to an environment consisting of either filtered air or cigarette smoke for 6 months. Smoke exposure (7 hours per day, 5 days per week) was standardized using a model TE-10 smoking machine and adjusted to maintain constant sidestream and mainstream smoke. After 6 months of exposure, we assessed differences in lung air space, cholesterol, lipid, and lipoprotein profiles, as well as paraoxonase activity in mice exposed to cigarette smoke extract compared to unexposed control mice.Results: Cigarette smoke exposure by the protocol used was sufficient to result in pathologic changes in lung architecture consistent with emphysema. Specifically, we observed that mice exposed to cigarette smoke had a significantly higher mean linear chord length compared to animals that were exposed to filtered air (p<0.02). Despite this exposure, no differences in total HDL-cholesterol levels or HDL-cholesterol sub-fractions (i.e. HDL2 and HDL3 fractions) were noted between smoke-exposed and unexposed animals (p=1.00, 0.6, and 0.4, respectively). Notably, mean HDL-cholesterol levels were identical between groups (92.8 vs 92.8 mg/dL, p=1.0). Paraoxonase activity, however, was markedly reduced in mice exposed to cigarette smoke compared to those who were not exposed (102, SD=9.6 vs 144, SD=4.1 units of activity, respectively, p=0.002).Conclusion: In this murine model, tobacco smoke exposure directly inhibits paraoxonase activity independently of HDL-cholesterol levels rather than indirectly via reduction in HDL-cholesterol levels.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"20-25"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35018519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tobacco Smoke Exposure Reduces Paraoxonase Activity in a Murine Model 烟草烟雾暴露降低小鼠模型中的对氧磷酶活性

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01 DOI: 10.59566/ijbs.2017.13020

R. Reed, S. Borgan, M. Eberlein, M. Goldklang, Joshua Lewis, Michael Miller, M. Navab, B. Kim

{"title":"Tobacco Smoke Exposure Reduces Paraoxonase Activity in a Murine Model","authors":"R. Reed, S. Borgan, M. Eberlein, M. Goldklang, Joshua Lewis, Michael Miller, M. Navab, B. Kim","doi":"10.59566/ijbs.2017.13020","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13020","url":null,"abstract":"AIM: To demonstrate a direct inhibitory effect of cigarette smoke exposure on paraoxonase 1 activity in a murine in vivo model. METHODS: At 8 weeks old, we randomized 10 C57/bl6 mice to an environment consisting of either filtered air or cigarette smoke for 6 months. Smoke exposure (7 hours per day, 5 days per week) was standardized using a model TE-10 smoking machine and adjusted to maintain constant sidestream and mainstream smoke. After 6 months of exposure, we assessed differences in lung air space, cholesterol, lipid, and lipoprotein profiles, as well as paraoxonase activity in mice exposed to cigarette smoke extract compared to unexposed control mice. RESULTS: Cigarette smoke exposure by the protocol used was sufficient to result in pathologic changes in lung architecture consistent with emphysema. Specifically, we observed that mice exposed to cigarette smoke had a significantly higher mean linear chord length compared to animals that were exposed to filtered air (p<0.02). Despite this exposure, no differences in total HDL-cholesterol levels or HDL-cholesterol sub-fractions (i.e. HDL2 and HDL3 fractions) were noted between smoke-exposed and unexposed animals (p=1.00, 0.6, and 0.4, respectively). Notably, mean HDL-cholesterol levels were identical between groups (92.8 vs 92.8 mg/dL, p=1.0). Paraoxonase activity, however, was markedly reduced in mice exposed to cigarette smoke compared to those who were not exposed (102, SD=9.6 vs 144, SD=4.1 units of activity, respectively, p=0.002). CONCLUSION: In this murine model, tobacco smoke exposure directly inhibits paraoxonase activity independently of HDL-cholesterol levels rather than indirectly via reduction in HDL-cholesterol levels.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"117 1","pages":"20 - 25"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77032442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Forensic Odontology: The New Dimension in Dental Analysis. 法医牙科学:牙齿分析的新维度。

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01

K P Divakar

引用次数: 0

Telescopic Overdenture: A Case Report. 套筒覆盖义齿1例报告。

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01

C S Shruthi, R Poojya, Swati Ram, Anupama

{"title":"Telescopic Overdenture: A Case Report.","authors":"C S Shruthi, R Poojya, Swati Ram, Anupama","doi":"","DOIUrl":"","url":null,"abstract":"Patient: This report describes the case of a 68 year old female patient who presented with the chief complaint of difficulty in chewing and poor aesthetics due to missing teeth. The patient was interested in saving the remaining natural teeth and desired minimal tissue coverage from the prosthesis. After consideration of all the factors involved, it was deemed advisable to resort to a palate free maxillary telescopic complete denture and a mandibular removable partial denture.Discussion: Considering the age of the patient and the cost involved, implant supported prosthesis was ruled out as a treatment option for the patient. A telescopic denture was chosen as a favourable treatment option since it overcomes many of the problems posed by conventional complete dentures like progressive bone loss, lower stability and retention, loss of periodontal proprioception and low masticatory efficiency. It also provides minimal tissue coverage and better distribution of forces. Evaluation of occlusion, esthetics, phonetics and comfort after 24 hours, 1 week and 1 month of treatment showed that the patient was happy with the prosthesis and was able to speak and chew well.Conclusion: Telescopic overdentures have better retention and stability as compared to conventional complete dentures. They improve the chewing efficiency, patient comfort and also decrease the alveolar bone resorption. As such they are an excellent alternative to conventional complete denture treatment.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"13 1","pages":"43-47"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5422644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35019032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Expression Profiling associated with Hepatoxicity in Pregnant Rats treated with Ubi Gadong (Dioscorea hispida) Extract 育碧加冬提取物与妊娠大鼠肝毒性相关的基因表达谱

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01 DOI: 10.59566/ijbs.2017.13026

Ezarul Faradianna Lokman, H. Muhammad, N. Awang, Maizatul Hasyima Omar, F. Mansor, Fatin Saparuddin

{"title":"Gene Expression Profiling associated with Hepatoxicity in Pregnant Rats treated with Ubi Gadong (Dioscorea hispida) Extract","authors":"Ezarul Faradianna Lokman, H. Muhammad, N. Awang, Maizatul Hasyima Omar, F. Mansor, Fatin Saparuddin","doi":"10.59566/ijbs.2017.13026","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13026","url":null,"abstract":"Dioscorea hispida (D.hispida) is the most well-known starchy tuber in Malaysia and called ‘ubi gadong’. Despite concerns over toxicity effects, the tuber is known to possess therapeutic values due to the presence of bioactive compounds such as saponins. This study was performed to identify the changes in gene expression profiles associated with hepatoxicity in pregnant rat treated with D.hispida using RT² Profiler PCR Array. The identification of steroidal saponins from D.hispida was carried out by UHPLC/MS method. Treatment of D.hispida caused mortality when dosage above 2000 mg/kg b.w. was given to pregnant rats. The PCR array showed that several genes were significantly up and down-regulated upon treatment with D.hispida. Treatment of D.hispida at 2000 mg/kg b.w leads to significant upregulation of several genes such as Btg2, Gsr, L2hgdn, S100a8, Slc17a3, Bhmt, Cd68, Cyp1a2 whereas several genes were downregulated such as Abcb1a, Aldoa, Cdc14b, Icam1, Krt18, Hpn and Maob. The consumption of D.hispida extract when taken at lower dosage of 2000 mg/kg may not be harmful to rats. D.hispida extract given at the highest dosage to pregnant rats caused alterations of several genes categorized in different hepatotoxic group functions such as necrosis, cholestasis and phospholipodisis.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"47 1","pages":"26 - 34"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77937406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

E-Cadherin and FGFR1 Expression in Mouse Osteoblastogenesis in Normoxic Cultures E-Cadherin和FGFR1在常压培养小鼠成骨细胞形成中的表达

International Journal of Biomedical Science : IJBS Pub Date : 2017-03-01 DOI: 10.59566/ijbs.2017.13013

Osama M Al-Amer

{"title":"E-Cadherin and FGFR1 Expression in Mouse Osteoblastogenesis in Normoxic Cultures","authors":"Osama M Al-Amer","doi":"10.59566/ijbs.2017.13013","DOIUrl":"https://doi.org/10.59566/ijbs.2017.13013","url":null,"abstract":"E-cadherin is a cell surface adhesion molecules that play an important role in tissue differentiation. FGFR1 is expressed in the developing and mature skeleton in patterns suggestive of both unique and redundant function. Expression levels of E-cadherin and FGFR1 during osteoblastogenesis unclear. In this study primary calvarial mouse osteoblasts were differentiated to mature osteoblasts in osteogenic medium. Alkaline phosphatase (ALP) activity, alizarin red staining, gene expression (Runt-related transcription factor 2 (Runx2), collagen 1 (COL1A2), osteocalcin, E-cadherin and FGFR1) and protein expression (E-cadherin and FGFR1) of osteogenic-cultured primary mouse osteoblast were analysed in this study. The osteogenesis capacity of primary osteoblasts was significantly promoted as ALP activity, alizarin red staining, and the relative expression of Runx2 mRNA and COL1A2 mRNA significantly increased during osteoblastogenesis. The results demonstrated that E-cadherin mRNA and protein were expressed in immature osteoblasts (day 7), but not in mature osteoblasts (day 28). In contrast, the expression of FGFR1 mRNA and protein significantly highly expressed in mature osteoblasts (day 28) compared with immature osteoblasts (day 7). In conclusion, this study demonstrated that E-cadherin could be used as a marker for immature osteoblasts, whereas FGFR1 could be used as a marker for mature osteoblasts during in vitro osteoblastogenesis.","PeriodicalId":13852,"journal":{"name":"International Journal of Biomedical Science : IJBS","volume":"35 5 1","pages":"13 - 19"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79626446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1