International Journal of Biological and Chemical Sciences最新文献

{"title":"Nutritional, functional properties and estimated shelf-life of defatted Rhynchophorus phoenicis (Fabricius, 1801) larvae flours","authors":"Nina Natacha Sophie Eyenga Ngono, Rodrigue Aymard Mba Fogang, Pauline Mounjouenpou, G. Kansci","doi":"10.4314/ijbcs.v17i6.24","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.24","url":null,"abstract":"Rhynchophorus phoenicis (Fabricius, 1801) is an insect of the coleopteran family widely consumed in Africa and particularly in Cameroon. Their dehydrated larvae are rich in lipids and proteins that can help in combating malnutrition. The present work aimed at producing low-lipid content larvae flours of R. phoenicis and to determine their nutritional and functional properties as well as their estimated shelf-life. The flour was obtained by the cooking-pressing process. The shelf-life of the flour was estimated using adsorption isotherms. The results showed that the flours obtained had low lipids (8 g/100 g of flour) and high protein content (69 g/100 g of flour). Their water and oil absorption capacities, swelling power, foaming properties and foam stability make them good ingredients for food formulations. As regards of storage capacity, the adsorption isotherms obtained can be modelled by the Guggenheim-Anderson-and-de-Boer (GAB) and Brunauer-Emmett-and-Teller (BET) equations. The shelf-life was estimated at 300 days when flour is packed in polyethylene bag and stored at 20°C in enclosure with a relative humidity of 90%. In short, the cooking pressing process makes it possible to obtain flours of high nutritional and technological values. The resulting flours can be used as ingredients to fortify food with poor proteins content.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"116 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etude ethnobotanique sur les niveaux de connaissance et d’usage d’Irvingia gabonensis, de Beilschmiedia mannii, et de Strombosia pustulata dans l’écosystème autour du Parc National de Taï (Sud-Ouest de la Côte d’Ivoire)","authors":"César Kouassi Yao, Marie-Thérèse N'dri Kouame, A. Mangara, Stanislas Georges Ablegué Bohi","doi":"10.4314/ijbcs.v17i6.10","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.10","url":null,"abstract":"Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill. (Irvingiaceae), Beilschmiedia mannii (Meism) Benth. & Hook (Lauraceae) et Strombosia pustulata. Hooker, (Olacaceae) sont des espèces végétales forestières qui sont très appréciée pour leurs graines comestibles. Les communautés locales utilisent les produits forestiers non ligneux de ses plantes sauvages à des fins alimentaires, médicinales et artisanales. L’objectif de la présente étude était de contribuer à la connaissance et à la valorisation de trois espèces (Irvingia gabonensis, de Beilschmiedia mannii et de Strombosia pustulata) menacées et utilisés par les riverains vivant à la périphérie du Parc National de Taï. Des enquêtes ethnobotaniques utilisant les méthodes de Walk-in the-woods interview, d’enquête individuelle et d’interviews semi-structurées ont été menées. Au total, 283 personnes ont été interrogées dans les onze villages d’étude dans le département de Taï. Ces plantes alimentaires appartenant à 3 familles ont fait l’objet d’étude. Les fruits sont les parties relativement les plus utilisés et les feuilles et les racines viennent en seconde position avec les mêmes pourcentages (33,33%) de consommation des trois espèces. Certaines de ces plantes servent également comme médicament et comme source de revenus pour les communautés locales. \u0000Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill. (Irvingiaceae), Beilschmiedia mannii (Meism) Benth. & Hook (Lauraceae) and Strombosia pustulata. Hooker, (Olacaceae) are forest plant species which are highly valued for their edible seeds. Local communities use the non-timber forest products of its wild plant to food, medicinal and artisanal purposes. The objectives of this study was to contribute to the knowledge and enhancement of three species (Irvingia gabonensis, Beilschmiedia mannii and Strombosia pustulata) threatened and used by residents living on the outskirts Taï National Park. Ethnobotanical surveys using Walk-in the-woods interview, individual survey and semi-structured interview methods were conducted. A total of 283 people were interviewed in the eleven study villages in the department of Taï. These food plants belonging to 3 families have been studied. The fruits are the relatively most used parts and the leaves and roots come in the second place with the same percentage (33,33%) of consumption of the three species. Some of these plants also serve as medicine and as a source of income for local communities.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"105 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the anti-salmonellal and antiplasmodial properties of leaf extracts of Rourea coccinea Beninese medicinal plant","authors":"Bénédicta Kpadonou-Kpoviessi, Basile Goueti, Hyacinthe F. Agnimonhan, Raoul Kemzeu, Bardieu Atchade, P. D. Toukam, Léon Ahoussi, Salomé Kpoviessi","doi":"10.4314/ijbcs.v17i6.21","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.21","url":null,"abstract":"Rourea coccinea, also called Byrsocarpus coccineus is a medicinal plant widely used in primary health care in West Africa and in this case in Benin. In the present study, the hydroethanolic extract of these leaves was investigated for its anti-salmonella and antiplasmodial properties. The evaluation of anti-salmonella activity was carried out by the micro dilution method associated with resazurine while that of antiplasmodial activity by method described by Syber Green. Minimum inhibitory concentrations (MICs) of the hydroethanolic extract against Salmonella strains were higher than 2000 μg/mL. This extract was active against multidrug-resistant Plasmodium falciparum strains (PfDd2) with an inhibitory concentration (IC50) of 32.26 μg/mL. These works on Rourea coccinea justify that the plant has a clearly remarkable antiplasmodial activity rather than anti-salmonella one and its use in traditional medicine to treat malaria in Benin.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"104 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation de la qualité des eaux de forage de la commune de Ouagadougou, Burkina Faso","authors":"A. Sawadogo, H. Cissé, François Tapsoba, Boureima Kagambèga, O. Zongo, L. Ouedraogo, Cheikna Zongo, Aly Savadogo","doi":"10.4314/ijbcs.v17i6.32","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.32","url":null,"abstract":"L’eau est un élément vital pour tous les êtres vivants. Elle peut provenir de plusieurs sources dont les forages. Les eaux de forage sont souvent exposées à de nombreuses contaminations, elles peuvent donc présenter un risque pour la santé des consommateurs. Ainsi, cette étude s’était fixée pour objectif d’évaluer la qualité physico-chimique et microbiologique des eaux de forage de la commune de Ouagadougou. Pour cela, des prélèvements ont été effectués au niveau de 14 forages. Les caractéristiques des échantillons prélevés ont été déterminées en utilisant des méthodes spectrophotométrique, titrimétrique, volumétrique, colorimétrique et microbiologique. Les résultats des analyses physico-chimiques ont montré que tous les échantillons présentaient des valeurs conformes aux normes pour 11 des paramètres étudiés. Aucun échantillon ne présentait une température conforme. De plus, quelques échantillons présentaient des valeurs non conformes pour le pH et le taux de chlore libre. Les analyses microbiologiques ont révélé une absence de streptocoques fécaux dans tous les échantillons. Les coliformes étaient absents dans la plupart des échantillons sauf dans celui de Sandogo (SASE6) (4 UFC /100 ml et 2 UFC /100 ml respectivement pour les coliformes totaux et fécaux). Selon la norme OMS, les échantillons d’eaux étudiés sont de qualité microbiologique satisfaisante sauf l’échantillon SASE6. \u0000Water is a vital element for all living beings. It can come from several sources including boreholes. Boreholes water is often exposed to many contaminations, so it can pose a health risk to consumers. Thus, this study aimed to evaluate the physico-chemical and biological quality of borehole water in the municipality of Ouagadougou. For this purpose, samples were taken from 14 boreholes. The characteristics of the samples taken were determined using spectrophotometric, titrimetric, volumetric, colorimetric and microbiological methods. The results of the physico-chemical analyses showed that all the samples presented values in conformity with the standards for 11 of the studied parameters. None of the samples had a temperature value in conformity with the standards. In addition, some samples showed non-compliant values for the pH and the free chlorine level. Microbiological analyzes revealed an absence of faecal streptococci in all samples. Coliforms were absent in most of the samples except in that of Sandogo (SASE6) (4 CFU/100 ml and 2 CFU/100 ml respectively for total and faecal coliforms). According to the WHO standard, the water samples studied are of satisfactory microbiological quality except for the SASE6 sample.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"124 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agronomic evaluation of some progenies resulting from crosses between double self-fertilised genitors of Deli and La Mé origins used in oil palm (Elaeis guineensis Jacq.) seed production in Côte d'Ivoire","authors":"Mêgbê Soumahoro, J. Konan, M. Boye, Gilles Léonce Niamketchi, Vamara Paterne Fofana","doi":"10.4314/ijbcs.v17i6.5","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.5","url":null,"abstract":"Progeny tests carried out between self-fertilised double genitors (DA 115 D AF AF and LM 2 T AF AF) used in seed production in the C 1001 F category were set up in Côte d'Ivoire in 2005, in order to identify crosses producing good oil yields and capable of adapting to current climatic conditions. The aim of this study was to assess the agronomic values and growth rate of palm height of these progenies compared with two first-cycle controls, LM 2 T x DA 10 D and LM 2 T x DA 115. Their susceptibility to Fusarium wilt figurewas also assessed. The results of these tests showed a low bunch yield of around 99% on the one hand, and a 115% increase in oil yield compared with the first cycle on the other. In addition, two progenies, LM 25759 and LM 25753, performed exceptionally well, with oil yields of 125% and 127% respectively and a 97% reduction in palm height growth rate compared with the first cycle. These two progenies were also tolerant to Fusarium wilt with Fusarium index of 40 and 79 respectively.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"121 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of heat treatment on physico-chemical parameters and extractability of free radical scavengers from Hibiscus sabdarifa juice","authors":"M. Balde, Harouna Tirera, I. Ndoye, Rokhaya Gueye, Adama Diédhiou, Yoro Tine, D. Fall, M. Seck, Amadou Diop, A. Fall, S. Sarr, A. Wélé","doi":"10.4314/ijbcs.v17i6.33","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.33","url":null,"abstract":"Hibiscus sabdariffa also known as Roselle is a shrub generally measuring between one and two meters in height. After flowering, white or red calyxes appear, depending on the variety. These calyxes are highly prized by African populations for the production of drinking juice. Thus, this work was initiated to study the thermal effects on physico-chemical parameters of the juice obtained. The pH was determined using a pH meter, the conductivity using a conductivity meter. Polyphenols were quantified by the ferric chloride reaction and Folin-Ciocalteu's reagent. The antiradical activity was determined by the ABTS method and the mineral content was determined using an atomic absorption spectrometer. According to the results, the production of juice at 100°C allowed the extraction of more phytochemical compounds among which: flavonoids (66.6 mg EQ/100 g dry extract) and polyphenols (49.3 mg EAG/100 g dry extract). The results also showed a better anti-free radical capacity for the juice obtained at 100°C compared to the other juices in this study. Indeed, the highest percentage of inhibition was obtained with the lowest concentration of juice extracted at 100°C (0.446 mg/mL). Hence, this study allowed the determination of the optimal temperature for preparing hibiscus sabdarifa juice.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"111 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure des Bulbuls du Parc National du Banco (Abidjan, Côte d’Ivoire) et effet de l’architecture des arbres sur leur distribution spatiale","authors":"K. P. Kouadio, Essé Savorgnan Assa, Tih Mathieu Koue-Bi, Hilaire K. Yaokokore-Beibro","doi":"10.4314/ijbcs.v17i6.12","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.12","url":null,"abstract":"Les Bulbuls ou Pycnonotidae constituent l’une des familles d’oiseaux assez diversifiée qui occupent divers habitats. Ils sont dépendant de la structure et de la composition de la végétation. Mais aucune donnée n’est publiée sur l’effet de l’architecture des arbres sur leur distribution en milieu forestier. En vue de combler cette insuffisance, cette étude a été menée de février 2012 à janvier 2013 dans le parc national du Banco. L’objectif a été de connaître leur structure et la relation entre eux et l’architecture des arbres dans six faciès d’habitats. Ainsi par la méthode de l’indice ponctuel d’abondance, les Bulbuls ont été échantillonnés de même que les arbres selon leur architecture dans un rayon de 50 mètres autour de chaque point d’écoute. Au total 20 espèces de Bulbuls d’une abondance ponctuelle de 1047 individus ont été recensées. Parmi elles, deux sont inscrites sur la liste rouge de l’UICN : le Bulbul à barbe jaune Criniger olivaceus et le Bulbul à queue verte Bleda eximius. L’analyse à redondance canonique a révélé que la distribution des Bulbuls varie selon l’architecture des arbres et des habitats. Ainsi, neuf espèces de Pycnonotidae sont positivement et significativement influencées (p < 0,05) par les arbres à architectures de types B, C et D. Par contre, les autres espèces de Bulbuls n’en sont pas, sauf le bulbul verdâtre Eurillas virens et le bulbul des raphias Thescelocichla leucopleura qui en sont négativement corrélées. \u0000The Bulbuls or Pycnonotidae are one of the fairly diverse bird families that occupy various habitats. They are dependent on the structure and composition of vegetation. But no data is published on the effect of tree architecture on their distribution in forest environments. In order to fill this gap, this study was conducted from February 2012 to January 2013 in Banco National Park. The objective was to know their structure and the relationship between them and the architecture of the trees in six habitat facies. Thus, by the method of the point index of abundance, the Bulbuls were sampled as well as the trees according to their architecture within a radius of 50 meters around each count point. A total of 20 species of Bulbuls with a point abundance of 1047 individuals have been recorded. Among them, two are on the IUCN Red List: the Yellow-bearded Greenbul Criniger olivaceus and the Green-tailed Bristlebill Bleda eximius. Canonically redundant analysis revealed that the distribution of bulbuls varies according to the architecture of trees and habitats. Thus, nine species of Pycnonotidae are positively and significantly influenced (p < 0.05) by trees with type B, C and D architectures. On the other hand, the other species of Bulbuls are not, except the Little Greenbul Eurillas virens and the Swamp Palm Bulbul Thescelocichla leucopleura which are negatively correlated.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"109 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revue des usages traditionnels, composition chimique et propriétés pharmacologiques de Combretum glutinosum (Combretaceae)","authors":"Y. Tine, Madièye Sene, K. Thiam, C. Gaye, A. Diallo, Benjamin Ndiaye, I. Ndoye, Adama Diedhiou, M. Balde, M. Seck, Djibril Fall, A. Wélé","doi":"10.4314/ijbcs.v17i6.27","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.27","url":null,"abstract":"Combretum glutinosum Perr. ex DC. (Combretaceae) est une plante médicinale largement utilisée en Afrique par les praticiens traditionnels pour le traitement de divers troubles comme les maladies respiratoires, la douleur, le paludisme et les infections microbiennes. En raison de sa large utilisation traditionnelle, dans cette revue, les rapports scientifiques publiés sur sa composition et ses propriétés pharmacologiques ont été explorés en effectuant une recherche documentaire dans les bases de données de Google Scholar, PubMed, Elsevier, ScienceDirect et Scifinder. Les études chimiques ont montré la présence de saponines, de flavonoïdes, d’alcaloïdes, de tanins, de saponines, de glycosides cardiaques, d’anthraquinones, d’acides aminés et de terpénoïdes. Il a été démontré que des extraits de cette plante ainsi que les composés purs qui en ont été isolés, possédaient des propriétés antibactérienne, antiparasitaire, antidiarrhéique, cytotoxique, anti-inflammatoire, hypoglycémiante, antioxydante, antihelminthique et antipaludique, entre autres. Cette revue fournit donc une vue d'ensemble des progrès actuels en matière, d'ethnopharmacologie, de phytochimie, de pharmacologie et de toxicité de C. glutinosum, constituant ainsi une référence pour la Recherche et Développement sur C. glutinosum. \u0000Combretum glutinosum Perr. ex DC. (Combretaceae) is a medicinal plant widely used in Africa by traditional practitioners for the treatment of various disorders such as respiratory diseases, pain, malaria and microbial infections. Due to its wide traditional use, in this review, published scientific reports on its composition and pharmacological properties were explored by conducting a literature search of Google Scholar, PubMed, Elsevier, ScienceDirect and Scifinder databases. Chemical studies showed the presence of saponins, flavonoids, alkaloids, tannins, saponins, cardiac glycosides, anthraquinones, amino acids, and terpenoids. Extracts of this plant, as well as the pure compounds isolated from it, have been shown to possess antibacterial, antiparasitic, antidiarrheal, cytotoxic, anti-inflammatory, hypoglycemic, antioxidant, antihelminthic and antimalarial properties, among others. This review therefore provides an overview of current progress in ethnopharmacology, phytochemistry, pharmacology and toxicity of C. glutinosum, thus constituting a reference for Research and Development on C. glutinosum.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"110 32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotype and genotyping of Salmonella sp. isolated from street foods in N’Djaména, Chad","authors":"Atéba Djibrine Mayoré, A. Bodering, Evariste Bako, N. Bessimbaye, Richard Gandolo, A. Tidjani, Nicolas Barro","doi":"10.4314/ijbcs.v17i6.3","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.3","url":null,"abstract":"Group foodborne illness infections with Salmonella are often caused by the simultaneous consumption by several people of heavily contaminated food. The objective of this work was to determine the phylogenetic relationships between dietary strains of Salmonella sp. isolated from our samples and confirmed clinical strains. A total of 447 samples were collected and analyzed using standard food microbiology methods. Enterobacterial Repetitive Intergenic Consensus-Polymerase chain Reaction (ERIC-PCR) technique allowed confirmation that all our strains belong to the genus Salmonella. From ten (10) strains characterized, eight (8) different genotype profiles were identified. The different DNA fragments of the isolates were generated by ERIC-PCR and then revealed on the agarose gel and had bands ranging from 2 to 6 bands with sizes between 150 and 1500 bp. The two food strains of S. Mbadaka (1 and 2) presented identical profiles. However, it is noted that two (2) strains of S. Anatum had different ERIC-PCR profiles. There is also a very high resemblance between the strain of S. Anatum (strain 5) and the confirmed clinical strains (H5: Salmonella Typhi). The presence of risky germs in the food analyzed poses a public health problem and should call on the competent authorities and prompt the taking of corrective measures.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"107 50","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and cytotoxic properties of Combretum paniculatum and Fadogia agrestis extracts on prostate and cervical cancers cell lines","authors":"B. Ouédraogo, B. Bayala, Boukaré Kaboré, Pengdwendé Fabienne Ingrid Zongo, Estelle Ouédraogo, Liz Laetitia Coulibaly, Marc Donald Wilfried Adico, F. Djigma, A. Yonli, Moumouni Koala, Jacques Simpore","doi":"10.4314/ijbcs.v17i6.2","DOIUrl":"https://doi.org/10.4314/ijbcs.v17i6.2","url":null,"abstract":"The medicinal plants used in Burkina Faso are essential source of research for bioactive compounds against prostate and cervical cancer, which are major health problems in Burkina Faso and worldwide. This was done by hydro-acetic maceration of the powder of the leafy branches of Combretum paniculatum (C. paniculatum) and Fadogia agrestis (F. agrestis). These extracts were then fractionated using petroleum ether, dichloromethane, ethyl acetate, and n-butanol. A phytochemical screening of these extracts was performed using High-Performance Thin Layer Chromatography (HPTLC). Antioxidant activity was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH∎) and 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS∎+). The extracts cytotoxicity was assessed by the MTT (Mitochondrial Tetrazolium Test) method on the LNCaP cell lines of prostate cancer and HeLa of cervical cancer. Phytochemical analysis of extracts and fractions revealed the presence of tannins, flavonoids, and steroids in these plants. For antioxidant activity, the ethyl acetate fraction of F. agrestis and the n-butanol fraction of C. paniculatum gave the best inhibition of DPPH∎ with IC50 values of 0.246 ± 0.045 and 0.097 ± 0.008 μg extract/mL, respectively. Using the ABTS∎+ method, the ethyl acetate fractions of C. paniculatum (1.1009 ± 0.0181 μM ET/g) and F. agrestis (0.0316 ± 0.0007 μM ET/g) showed the best activity. In addition, the fractions of dichloromethane (DCM) of F. agrestis (IC50 = 24.69 ± 9.51 μg/ml) and ethyl acetate (AE) of F. agrestis (IC50 = 129.9 ± 6.49 μg/ml) had better activity, respectively on the prostate and cervical cancer LNCaP lines. These properties could justify the use of these plants in traditional medicine.","PeriodicalId":13808,"journal":{"name":"International Journal of Biological and Chemical Sciences","volume":"105 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}