International Journal of Current Microbiology and Applied Sciences最新文献

{"title":"Predominance of Wild K13 Propeller Haplotypes in three localities of Côte d'Ivoire one decade after the adoption of Therapeutic Combinations based on Artemisinin Derivatives","authors":"Dagnogo Oléfongo, K. Rolland, A. Bérenger, Bla Kouakou Brice, Touré Offianan André, Djaman Allico Joseph","doi":"10.20546/ijcmas.2023.1206.010","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.010","url":null,"abstract":"Background: The emergence of P. falciparum resistance to artemisinin-based combination therapy (ACT) threatens malaria control in Africa. The monitoring of polymorphisms in molecular markers associated with antimalarial drug resistance is essential for malaria control and elimination efforts. The purpose of this study is to analyze the polymorphism of the pfK13 propeller gene of P. falciparum to artemisinin-based combination therapy (ACT) in three sites in southern Côte d'Ivoire. Methods: After obtaining informed consent, blood samples were collected from patients of any sex and aged over 2 years with uncomplicated P. falciparum malaria in Anonkoua-Kouté, Port-Bouët and Ayamé. P. falciparum genomic DNA was extracted and amplified by Nested-PCR using Pfk13 propeller gene-specific primers. The amplification products were sequenced using the Sanger method at Eurofins Genomics. The key codons (493, 539, 543 and 580), molecular markers of the resistance of P. falciparum to ACTs and the additional codons (476 and 561) were analyzed. Results: A total of 186 DNA extracts from blood samples collected from the three study sites were amplified and sequenced. Of the 93 PCR products sequenced, 84.94% (158/186), 91.39% (170/186), 95.69% (178/186), 100% (186/186), 82.79% (154/186) and 94.62% (178/186) corresponding to codons 493, 539, 543, 580, 476, 561, respectively, were successfully analyzed. Thus, the prevalences of wild-type alleles were 92.40% (Tyr493), 94.11% (Arg539), 94.38% (Ile543), 91.39% (Cys580), 89.61% (Met476) and 88.63% (Arg561) against 1.26% (His493), 3.52% (Thr539) and 1.29% (Ile476) of mutant alleles. The mutant alleles Thr543, Tyr580, and His561 were not observed in all three study sites. The analysis indicated a predominance of the YRICMR allelic form representing a susceptible haplotype (78.49%). No significant difference was observed between the prevalences of the wild-type alleles determined in the three study sites (p>0.05). Conclusion: More than a decade after the adoption of ACTs for the management of uncomplicated malaria, these combinations are still effective in Anonkoua-Kouté, Port-Bouët and Ayamé in the south of Côte d'Ivoire.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91055474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socio-Economic Status of Wheat Growers in Dehradun District of Uttarakhand","authors":"Kokab Askari, Amit Kumar Masih, Vikas Singh","doi":"10.20546/ijcmas.2023.1206.017","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.017","url":null,"abstract":"In order to determine their socioeconomic features, a survey was undertaken on 185 wheat producers from small, medium, and large farm groups in the Vikasnagar and Sahaspur blocks of Dehradun district. The study's findings showed that the majority of respondents had average agricultural land holdings per hectare for small-scale operations of 1.57 hectare, 2.60 hectare for medium-scale operations, and 12.46 hectare for large-scale operations, which together made up an average sample of 4.29 hectare. Farm families' average sizes in the categories of small, medium, and large farms were 4.89, 5.15, and 5.66, respectively. For groups of farms of various sizes, the average sample proportion of males and females was 46.89% and 44.79%, respectively. The age composition of farms of various sizes with the highest average sample percentage is comprised of individuals under the age of 29 (52.12%), followed by those between the ages of 30 and 59 (30.95%), and those who are 60 years and over (7.79%). The percentage of literate farmers was highest in small-scale farms (25.97%), followed by medium-scale farms (23.50%), and lowest in large-scale farms (22.44%). For various size farm groups, the average sample was 24.30%.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90435683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pest Diversity in Sweet Corn [Zea mays saccharate (Sturt.) Bailey] in East Denpasar, Bali, Indonesia","authors":"Made Sudarma, Ni Nengah Darmiati, Ni Wayan, Suniti","doi":"10.20546/ijcmas.2023.1206.022","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.022","url":null,"abstract":"Sweet corn (Zea mays saccharate) is cultivated in Denpasar, Bali, which is harvested when it is young for daily use, both for vegetables and for consumption, boiled or grilled. Most of the pests found were borers (Ostrinia furnacalis, Crambidae, Lepidoptera), flies (Musa domistica, Muscidae, Diptera), grasshoppers (Locusta sp., Acrididae, Orthoptera) and rats (Rattus rattus, Muridae, Rodentia). The pest diversity index ranged from 0.519-1.003, the pest dominance index ranged from 0.269-0.600 and the evenness index between species ranged from 0.094-0.700. This means that the diversity is very small, supported by a small evenness index, which also means that there are insect pests that dominate. Population dynamics of insect pests range from 0.10 to 0.21, which means the number of births and additions per day per hundred is 10 to 21 individuals. The relationship between pests and weather factors (temperature and humidity) only for borers (located on Jalan Sekarsari) has a significant positive correlation of 0.77* with the regression equation Y = 0.348 X – 24.356 (R2 = 0.71, X = temperature and Y = borer population).","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84409896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Pod Coating Formulation With the Carrier Media of Trichoderma asperellum in Controlling Phytophthora Pod Rot Disease","authors":"Eka Wisdawati, Kafrawi Zahraeni Kumalawat, Henny Poerwanty","doi":"10.20546/ijcmas.2023.1206.020","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.020","url":null,"abstract":"One of the important diseases in cocoa plants is pod rot disease caused by pathogen Phytophthora palmivora. Loss which caused pod rot disease varies between 26 % and 50 %.One of the biological agents which potential to control cocoa pod rot is a type of Trichoderma asperellum. There have been many uses of Trichoderma as a biofungicide, including direct spraying, mass propagation on rice media in the form of flour and with the addition of kaolin. This research aims to compare the effectiveness of using Trichoderma asperellum in several cocoa pod coating application techniques. Cocoa pods that had the heaviest attack intensity was on the penis (no T. asperellum treatment) which was 72.22% while the attack symptoms and the lowest disease attack intensity were the T. asperellum treatment, added kaolin and MCC, with an attack intensity of 5.56%. The weight of 100 cocoa beans in each treatment, the amount was not much different from the yield loss rate for the control, namely 17.63% and the treatment of cocoa pods sprayed with Trichoderma directly and pods sprayed with T. asperellum plus kaolin showed the amount of loss. The same yield was 13.29% and in the treatment of spraying T. asperellum which was added kaolin and CMC showed the lowest yield loss of 12.14%. The carrier media must contain important components to support the viability and growth of the microbes inoculated into it. This is because the carrier media functions to grow and extend the shelf life so that the carrier media must contain organic nutrients to support Trichoderma asperellum growth.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91292440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of Recipe for Preparation of Beverage Nectar from Dragon Fruit","authors":"Sonia Dansena, Shishir Prakash Sharma","doi":"10.20546/ijcmas.2023.1206.028","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.028","url":null,"abstract":"The current investigatory work entitled “Studies on Standardization of recipe for preparation of beverages Nectar of dragon fruit” was executed at Pomology Laboratory, Department of Pomology, Pt K.L.S. CHRS, Pendri, Rajnandgaon, IGKV, Raipur, Chhattisgarh during year 2019-2020. The research was performed on sensory analysis of Nectar of dragon fruit at fortnight meantime for course of 45 days preservation by implementing CRD with 3 replication inside lab condition. Each replication comprised of 7 treatments for Nectar. The nectar comprise of 20 percent pulp and 0.3 percent acidity and receipe is varied by different concentration of TSS which are as follows for T1 (16%TSS), T2 (17 % TSS), T3 (18 % TSS), T4 (19 %t TSS), T5 (20 % TSS), T6 (21%TSS), T7 (22 % TSS).Nectar with treatment T7 comprising of 20% pulp, 0.3% acidity and 22% TSS got peak score on colour, flavour, taste, appearance and overall acceptability among various recipe implemented. While it also obtained peak score in TSS, ascorbic acid, TSS Acid ratio, TS and RS is peak, while acidity and NR sugar was least for treatment T7 comprising of 20% pulp, 0.3% acidity and 22% TSS. NR sugar, and acidity got peak score in case of treatment T1 comprising of 20% pulp, 0.3% acidity and 16% TSS. The organoleptic score, pH, NR sugar and TSS acid ratio shows diminishing pattern whereas TSS, acidity, ascorbic acid, TS and RS shows rising pattern.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91441669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Region-Specific cut off value for Scrub Typhus IgM ELISA at a Tertiary Care Centre, Thanjavur, India","authors":"P. B. P. Kumar, J. K. Jeyabharathi, Eunice Swarna Jacob","doi":"10.20546/ijcmas.2023.1206.011","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.011","url":null,"abstract":"Rickettsial infections are primarily diagnosed using serological methods. The cut-off value for scrub typhus IgM ELISA is not standardized and varies depending on the disease prevalence in the geographical location where the kit is used. Therefore, establishing a region-specific cut-off value for diagnostic ELISA is crucial for the initial treatment of patients and distinguishing other febrile diseases in tertiary care settings. In this study, 300 patients from three different categories, including Category I (scrub typhus IgM ELISA positive patients), Category II (Dengue IgM ELISA positive patients), and Category III (100 healthy volunteers), were enrolled. Scrub typhus IgM ELISA (InBioS, International, Inc.) was performed, and the optical density (OD) values of all 300 samples were documented and analyzed using a receiver operating characteristic (ROC) curve. The analysis revealed a diagnostic IgM ELISA cut-off value of 0.728, with a sensitivity and specificity of 94% and 91%, respectively. Considering the significance of scrub typhus diagnosis for treatment and understanding disease dynamics in the Thanjavur district of Tamil Nadu, a cut-off value of >0.728 for diagnostic IgM ELISA can be utilized.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75024002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Environmental Hygiene and Microbiological Status of Contamination on Mobile phones from University of Kota","authors":"Palavi Sharma, Z. A. Ansari, N. Mahawar, A. Nimawat, S. Barolia","doi":"10.20546/ijcmas.2023.1206.004","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.004","url":null,"abstract":"Invasion of communication technology does not only bring dependence but also a major contribution of infection. Digital devices are possibly potential worldwide vectors of nosocomial infection. Mobile phone has increased the dependency irrespective of the geographical barriers, demographic factors like age, social and economic background. These digital devices have great prominence but they are also potential disease carrying elements. Mobile phones were collected and investigated for potential contamination of microbial flora. Total 25 Mobile phones sample were collected from different departments of university of kota and Sampled using a sterile cotton swab. Collected samples were cultured on Nutrient Agar, MH media and Mac-Conkey using streak plate’s method. The Sample phones collected include Mobile phones of Vice Chancellor, Faculty, Security Gaurd, Students, Sweepers, and Other employees. The Microbial species isolated include as Klebsiella pneumoniae, Actinobecter, Pseudomonas spp., Enterococcus spp, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida spp. 24 Out of 25 showed 100% contamination. This study provides all the necessary fact that Mobile phones are potential vectors of various diseases and lifestyle change should be adopted by every individual using mobile phone. Better sanitization practices and knowledge may prevent the spread diseases from Mobile Phones.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76073317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the Constraints in the Production and Marketing of Capsicum in the Solan District of Himachal Pradesh","authors":"Shantha Selva Kumari, Subhash Sharma, Harman Preet Kaur","doi":"10.20546/ijcmas.2023.1206.016","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.016","url":null,"abstract":"The present study was conducted with the objective to identify constraints in the production and marketing of Capsicum in the Solan District of Himachal Pradesh and to suggest measures to overcome them. Overall, 80 capsicum growers were selected for the study. Farmers were distributed according to land holding size, i.e., marginal, small, semi-medium and medium farmers. The study revealed that among various production and marketing problems, about 73 per cent of the farmers reported problems with diseases in the study area. Which showed significant differences between the different farm categories. The high transportation costs were the major problem faced by the farmers in marketing.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81504749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Cercarial Antigen Vaccine against Murine Schistosomiasis mansoni","authors":"Dina Ahmed Samy Elguindy, Dalia S. Ashour, Sirria M. Elmarhoumy, Dina M. Elguindy, H. Ismail","doi":"10.20546/ijcmas.2023.1206.001","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.001","url":null,"abstract":"Schistosomiasis is one of the most common parasitic diseases worldwide. Because of the low bioavailability of praziquantel and the appearance of drug resistant strains, the development of an effective vaccine is crucial to control schistosomiasis. To test the effect of cercarial antigen as a potential vaccine against Schistosoma mansoni -infected mice. Methods: 90 mice divided into 2 groups: group I: (Control group) (40 mice) subdivided into subgroup Ia (15 mice): Non-infected mice (normal control) and subgroup Ib (25 mice): Schistosoma infected mice (infected control). Group II: (50 mice) (The vaccinated group): received cercarial antigen then infected with S. mansoni three weeks after the first immunization dose. Vaccine efficacy was assessed by parasitological and histopathological studies. The vaccinated group caused significant reduction in adult worms count, intestinal egg countand intestinal granulomas size and number compared to the infected control group. Cercarial antigen vaccine is a promising schistosomal antigen that has a protective effect against Schistosoma mansoni as proved by parasitological and histopathological examination.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80179143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physico-Chemical Parameters of the Soil and Distribution of Forms of Resistance of Human Parasites in the Mbam and Inoubou Division (Bokito-Cameroon)","authors":"Mbassi Mbida Marcelle Blanche, Zebaze Togouet Serge Hubert","doi":"10.20546/ijcmas.2023.1206.013","DOIUrl":"https://doi.org/10.20546/ijcmas.2023.1206.013","url":null,"abstract":"This study was conducted to assess the physico-chemical parameters of soils and the distribution of resistance forms of soil-transmitted helminths and protozoa in the land surrounding the Okole and Lebomo streams in Bokito. The sedimentation and the Faust technics were used to identify and count the resistance forms of intestinal parasites. Among the 40 soil samples collected, 27 presented at least one parasite; for an overall contamination rate of 22%; thus distributed helminths (14%) and protozoa (8%). High values in parasite density were recorded during the short rainy season in Strongyloides stercoralis with a contamination frequency of 2, 1%. On the other hand, parasites such as Trichuris trichiura, Toxocara canis and Isospora belli were not observed during the short dry season. No significant spatio-temporal variation was observed for temperature, pH and dissolved oxygen. This result would be due to the absence of anthropogenic actions and the influence of environmental conditions.","PeriodicalId":13777,"journal":{"name":"International Journal of Current Microbiology and Applied Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79654079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}