Interdisciplinary Toxicology最新文献_第5页

In vitro cytogenotoxic evaluation of sertraline 舍曲林的体外细胞遗传学毒性评价

Interdisciplinary Toxicology Pub Date : 2018-10-01 DOI: 10.2478/intox-2018-0015

E. Istifli, Rima Çelik, Mehmet Tahir Hüsunet, Nesrin Çetinel, O. Demirhan, H. B. Ila

{"title":"In vitro cytogenotoxic evaluation of sertraline","authors":"E. Istifli, Rima Çelik, Mehmet Tahir Hüsunet, Nesrin Çetinel, O. Demirhan, H. B. Ila","doi":"10.2478/intox-2018-0015","DOIUrl":"https://doi.org/10.2478/intox-2018-0015","url":null,"abstract":"Abstract Sertraline (SRT) is an antidepressant agent used as a neuronal selective serotonin-reuptake inhibitor (SSRI). SRT blocks serotonin reuptake and increases serotonin stimulation of somatodendritic serotonin 1A receptor (5-HT1AR) and terminal autoreceptors in the brain. In the present study, the genotoxic potential of SRT was evaluated using cytokinesis-block micronucleus (CBMN) cytome assay in peripheral blood lymphocytes of healthy human subjects. DNA cleavage-protective effects of SRT were analyzed on plasmid pBR322. In addition, biochemical parameters of total oxidant status (TOS) and total antioxidant status (TAS) in blood plasma were measured to quantitate oxidative stress. Human peripheral blood lymphocytes were exposed to four different concentrations (1.25, 2.5, 3.75 and 5 µg/mL) of SRT for 24- or 48-h treatment periods. In this study, SRT was not found to induce MN formation either in 24- or 48-h treatment periods. In contrast, SRT concentration-dependently decreased the percentage of MN and MNBN (r=−0.979, p<0.01; r=−0.930, p<0.05, respectively) when it was present for the last 48 hr (48-h treatment) of the culture period. SRT neither demonstrated a cleavage activity on plasmid DNA nor conferred DNA protection against H2O2. The application of various concentrations of SRT significantly increased the TOS and oxidative stress index (OSI) in human peripheral blood lymphocytes for both the 24- and 48-h treatment periods. Morover, the increase in TOS was potent as the positive control MMC at both treatment times. However, SRT did not alter the TAS levels in either 24- or 48-h treatment periods when compared to control. In addition, exposing cells to SRT caused significant decreases in the nuclear division index at 1.25, 2.50 and 3.75 µg/mL in the 24-h and at the highest concentration (5 µg/mL) in the 48-h treatment periods. Our results suggest that SRT may have cytotoxic effect via oxidative stress on cultured human peripheral blood lymphocytes.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"181 - 188"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43621243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice 双酚A对小鼠附睾尾部剂量依赖性不良反应的体内分析

Interdisciplinary Toxicology Pub Date : 2018-10-01 DOI: 10.2478/intox-2018-0019

Sanman Samova, Hetal Doctor, R. Verma

{"title":"In vivo analysis of Bisphenol A induced dose-dependent adverse effects in cauda epididymis of mice","authors":"Sanman Samova, Hetal Doctor, R. Verma","doi":"10.2478/intox-2018-0019","DOIUrl":"https://doi.org/10.2478/intox-2018-0019","url":null,"abstract":"Abstract Bisphenol A is widely used as a material for the production of epoxy resins and polycarbonate plastics. It contaminates various food stuffs by getting leached out from their container lining. Limited information is available on its effects on the male reproductive system. The aim of the present study was to evaluate the extent to which bisphenol A can affect the reproductive system by measuring biochemical and histological changes in the epididymis. Inbred Swiss strain male albino mice were orally administered 80, 120 and 240 mg/kg body weight/day of BPA for 45 days. After completion of treatment, the animals were sacrificed; cauda epididymis was isolated, weighed, used for biochemical and histopathological studies. The results revealed that BPA administered for 45 days caused significant (p<0.05) and dose-dependent reduction in epididymis weight. There was significant (p<0.05) increase in lipid peroxidation and the acid phosphatase activity. Dose dependent reduction in protein, sialic acid contents, as well as the activity of enzymatic antioxidants and mitochondrial enzymes was recorded compared to vehicle treated group. The effect was dose-dependent. Histopathological alteration was observed. This study concludes that BPA causes toxicity in epididymis of mice by generating free radicals, which may be a possible reason for reduction in sperm parameters.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"209 - 216"},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43028685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antidotal effects of thymoquinone against neurotoxic agents 胸腺肽醌对神经毒性毒剂的解毒作用

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0008

A. Sanati, T. Farkhondeh, S. Samarghandian

引用次数: 7

Washing soda induced alteration of the differential cell count, nonself surface adhesion efficacy and nuclear morphology of the polyphenotypic cells of a freshwater sponge of India 洗涤钠诱导印度淡水海绵多表型细胞的差异细胞计数、非自身表面粘附效能和核形态的改变

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0012

S. Mukherjee, M. Ray, Sajal Ray

{"title":"Washing soda induced alteration of the differential cell count, nonself surface adhesion efficacy and nuclear morphology of the polyphenotypic cells of a freshwater sponge of India","authors":"S. Mukherjee, M. Ray, Sajal Ray","doi":"10.2478/intox-2018-0012","DOIUrl":"https://doi.org/10.2478/intox-2018-0012","url":null,"abstract":"Abstract Washing soda has been identified as a precarious contaminant of the freshwater ponds and lakes, the natural habitat of Eunapius carteri. Treatment of sublethal concentrations of washing soda for 384 hours exhibited a significant decrease in the densities of blast like cells, small and large amoebocytes. The percentage occurrence of granular cells and archaeocytes yielded a marked increase against the experimental concentrations of washing soda. Washing soda mediated alterations in the differential cell densities of E. carteri indicative of a state of physiological stress and an undesirable shift in the cellular homeostasis of the organism distributed in polluted environment. Experimental exposure of washing soda yielded a significant increase in the cellular dimensions of large amoebocytes and archaeocytes. Prolonged treatment with washing soda presented a gross reduction in nonself surface adhesion efficacy of E. carteri cells. Experimental concentrations of washing soda resulted in a dose dependent increment in the frequencies of binucleation and micronucleation in the cells of E. carteri. The data were indicative of a high level of genotoxicity of washing soda in E. carteri. The present investigation provides an important information base in understanding the toxin induced chemical stress on the archaic immune defense of a primitive urmetazoa.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"155 - 168"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48868172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure 皮肤接触毒死蜱后普通蟾蜍灰鼻虫的血液生物标志物

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0011

R. Lajmanovich, P. Peltzer, Andrés M. Attademo, C. Colussi, C. Martinuzzi

{"title":"Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure","authors":"R. Lajmanovich, P. Peltzer, Andrés M. Attademo, C. Colussi, C. Martinuzzi","doi":"10.2478/intox-2018-0011","DOIUrl":"https://doi.org/10.2478/intox-2018-0011","url":null,"abstract":"Abstract Chlorpyrifos (CPF) is a broad spectrum pesticide commonly used for insect control, has great affinity for lipids and is thus a potential for bioaccumulation in aquatic organisms. The aim of this study was to evaluate the toxicity of CPF using the common toad Rhinella arenarum via dermal uptake in plastic bucket to simulate their natural exposition in ponds. R. arenarum toads were exposed individually to solutions containing a nominal concentration of a commercial formulation of CPF insecticide (5 and 10 mg/L). Different enzyme biomarkers (BChE: butyrylcholinesterase, CbE: carboxylesterase, and CAT: catalase) were measured in blood tissue after exposition. The capacity of pyridine-2-aldoxime methochloride (2-PAM) to reverse OP-inhibited plasma BChE and the ratio of heterophils and lymphocytes (H/L) as hematological indicators of stress were also determined. The normal values of plasma B-sterases (BChE and CbE) were highly inhibited (until ≈ 70%) in toads 48 h after exposure to CPF. The results indicate that 2-PAM produced BChE reactivation as well. The activity of CAT was also inducted for dermal exposure at more than double of that in the control toads (CPF; 5 mg/L). H/L ratios did not reveal a significantly increased stress. The study suggests that CPF via dermal uptake induced neurotoxicity and oxidative stress in the common toad R. areanum. Thus, some blood biomarkers employed in our study (i.e. BChE, CbE, 2-PAM, and CAT) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to CPF.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"148 - 154"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48437405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dichlorvos toxicity: A public health perspective 敌敌畏毒性：公共卫生视角

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0009

H. Okoroiwu, I. Iwara

引用次数: 62

Prophylactic efficacy of some chemoprotectants against abrin induced lethality 几种化学保护剂对蓖麻毒素致死性的预防作用

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0013

N. Saxena, Y. Bhutia, O. Kumar, Pooja Phatak, R. Kaul

{"title":"Prophylactic efficacy of some chemoprotectants against abrin induced lethality","authors":"N. Saxena, Y. Bhutia, O. Kumar, Pooja Phatak, R. Kaul","doi":"10.2478/intox-2018-0013","DOIUrl":"https://doi.org/10.2478/intox-2018-0013","url":null,"abstract":"Abstract Abrin is a highly toxic protein produced by Abrus precatorius. Exposure to abrin, either through accident or by act of terrorism, poses a significant risk to human health and safety. Abrin functions as a ribosome-inactivating protein by depurinating the 28S rRNA and inhibits protein synthesis. It is a potent toxin warfare agent. There are no antidotes available for abrin intoxication. Supportive care is the only option for treatment of abrin exposure. It is becoming increasingly important to develop countermeasures for abrin by developing pre- and post-exposure therapy. The aim of this study is to screen certain pharmaceutical compounds for their chemoprotective properties against abrin toxicity in vivo in BALB/c male mice. Twenty-one compounds having either antioxidant, anti-inflammatory and cyto-protective properties or combination of them, were screened and administered as 1h pre-treatment followed by exposure of lethal dose (2×LD50, intraperitoneally) of abrin. To assess the protective efficacy of the compounds, survival and body weight was monitored. Fifteen compounds extended the survival time of animals significantly, as compared to abrin. The following five of these compounds, namely: Epicatechin-3-gallate, Gallic Acid, Lipoic Acid, GSH and Indomethacin extended the life time ranging from 6 to 9 days. These compounds also attenuated the abrin induced inflammation and enzymes associated with liver function, but none of them could prevent abrin induced lethality. The compounds offering extension of life could be useful to provide a time-window for other supportive treatment and could also be used as combinatorial therapy with other medical countermeasures against abrin induced lethality.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"169 - 177"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69214463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Glutathione S-transferase is a good biomarker in acrylamide induced neurotoxicity and genotoxicity 谷胱甘肽s -转移酶是丙烯酰胺诱导的神经毒性和遗传毒性的良好生物标志物

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0007

Sreenivasulu Dasari, M. Ganjayi, Balaji Meriga

引用次数: 19

Multiple giant cell formation – A consequence of type II pyrethroid intoxication 多种巨细胞形成- II型拟除虫菊酯中毒的后果

Interdisciplinary Toxicology Pub Date : 2018-08-01 DOI: 10.2478/intox-2018-0014

B. Bhushan, P. N. Saxena

引用次数: 0

Toxicity assessment of agrochemical Almix in Heteropneustes fossilis through histopathological alterations. 通过组织病理学改变评估农用化学品Almix在异呼吸菌化石中的毒性

Interdisciplinary Toxicology Pub Date : 2018-08-01 Epub Date: 2019-03-02 DOI: 10.2478/intox-2018-0010

Palas Samanta, Rituparna Das, Sandipan Pal, Aloke Kumar Mukherjee, Tarakeshwar Senapati, Debraj Kole, Apurba Ratan Ghosh

{"title":"Toxicity assessment of agrochemical Almix in Heteropneustes fossilis through histopathological alterations.","authors":"Palas Samanta, Rituparna Das, Sandipan Pal, Aloke Kumar Mukherjee, Tarakeshwar Senapati, Debraj Kole, Apurba Ratan Ghosh","doi":"10.2478/intox-2018-0010","DOIUrl":"10.2478/intox-2018-0010","url":null,"abstract":"The present study was designed to assess the adverse effects of the agrochemical Almix on comparative basis in gill, liver and kidney of Heteropneustes fossilis through histological and ultrastructural observations under field (8 g/acre) and laboratory (66.67 mg/L) conditions. Exposure duration of both experiments was 30 days. Gill showed atrophy in secondary lamellae, hypertrophied gill epithelium, damage in chloride and pillar cells, and detachment of chloride cells from gill epithelium under laboratory condition, but hypertrophy in gill epithelium and fusion in secondary lamellae were seen under field condition. In gill, scanning electron microscopy (SEM) showed fragmentation in microridges, hyper-secretion of mucus and loss of normal array in microridges, while transmission electron microscopy (TEM) displayed dilated mitochondria and rough endoplasmic reticulum (RER), abnormal sized vacuolation in chloride cells under laboratory condition. In liver, hypertrophied and pyknotic nuclei, disarrangement of hepatic cords, and cytoplasmic vacuolation were prominent under laboratory study but in field condition the liver showed little alterations. TEM study showed severe degeneration in RER and mitochondria and cytoplasmic vacuolation under laboratory condition but dilated mitochondria were prominent in field observation. Kidney showed severe nephropathic effects including degenerative changes in proximal and distal convolute tubule, damage in glomerulus under light microscopy, while deformity in nucleus, fragmentation in RER, severe vacuolation and necrosis in kidney were prominent under TEM study. The results clearly demonstrated that responses were more prominent in laboratory than field study. Thus the responses displayed by different tissues of concerned fish species exposed to Almix could be considered as indications of herbicide toxicity in aquatic ecosystem.","PeriodicalId":13715,"journal":{"name":"Interdisciplinary Toxicology","volume":"11 1","pages":"138-147"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42672152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0