{"title":"Effect of synthetic androgen treatment on the gonad of juvenile longtooth grouper (Epinephelus bruneus)","authors":"Yasuhisa Kobayashi, Takamasa Morishita, H. Chuda","doi":"10.22034/IAR.2021.1928726.1156","DOIUrl":"https://doi.org/10.22034/IAR.2021.1928726.1156","url":null,"abstract":"Commercially significant groupers are protogynous hermaphrodites. Since their sex change from female (ovary) to male (testis) occurred in a larger size and older age, capturing or maintaining males in the aquaculture farm is very difficult. In the present study, juvenile longthooth groupers (2+ years old) were implanted with two doses of 17alpha-methyltestosterone (MT) cholesterol pellet (2 mg or 5 mg MT kg-1 BW) for the induction of artificial sex reversal. Individuals were sampled at 30 and 80 days after treatment. All the control group fish had immature ovaries contained with primary oocytes and germ cells. Fish treated with MT for 30 days had sex-transitional gonad consisted of few primary oocytes and testicular tissues. By using immunohistochemistry, we identified the proliferating and apoptotic cells in the gonad of this sexual stage. After 80 days for MT treatment, gonads of all individuals were filled with germ cells at all stages of spermatogenesis, and the newly formed efferent duct contained active spermatozoa. Since sperm motility was confirmed, MT treatment successfully induced functional sex reversal in pre-pubertal longtooth grouper. This method may dramatically reduce the cost and time required for the production of longtooth grouper.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43693325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of arsenic and lead on glucose level and expression of ERK in air-breathing catfish, Heteropneustes fossilis","authors":"Kermi-u Tariang, Kitboklang Khongsti, B. Das","doi":"10.22034/IAR.2021.1923096.1136","DOIUrl":"https://doi.org/10.22034/IAR.2021.1923096.1136","url":null,"abstract":"Heavy metal contaminations may have devastating effects on the ecological balance of the environment and diversity of aquatic organisms. Furthermore, they cause great threat to the Indian aquatic ecosystem. In this study, we analyzed the effect of arsenic (As) and lead (Pb) on the blood glucose level and their possible involvement in the mitogen-activated protein kinase (MAPK) pathway in liver and muscle tissues of Heteropneustes fossilis. The catfish, H. fossilis, were exposed to an acute (35.09 ppm As2O3 and 66.20 ppm PbCl2 for ~96 h) and chronic (LC50/20th ppm of both As2O3 and PbCl2 for ~30 d) concentration of As and Pb. Thereafter, the blood glucose level and the extracellular signal-regulated protein kinase (ERK) expression level in liver and muscle tissues of the fish were analyzed. It was found that As and Pb caused hyperglycemia in H. fossilis. Both on acute and chronic treatment with As and Pb, no significant change in p-ERK1/2 expression level was found in the muscle tissue of H. fossilis, whereas, in the liver tissue, the p-ERK1/2 expression level showed a significant increase in both acute (96 h) and chronic treatment (10 d and 30 d) of As and Pb. Therefore, it can be concluded that As and Pb could be highly toxic to the aquatic fauna, which could be a potential threat to human health as well.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"129-137"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41620371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intra- and inter-specific variability of IGF-I expression in farmed cold and warm-water species in Iran","authors":"M. Haghi, M. Mohseni, M. Ghane","doi":"10.22034/IAR.2021.682480","DOIUrl":"https://doi.org/10.22034/IAR.2021.682480","url":null,"abstract":"Changes in the expression of liver insulin-like growth factor IGF-I mRNA in two growing cold-water fish species Oncorhynchusmykiss(one from locally produced and one from imported broodstock) and in two warm-water species, Hypophthalmichthysnobilis and Hypophthalmichthysmolitrix were analyzed. Gene expression analysis in liver tissues by using real-time PCR revealed a significant difference in expression level of IGF-I between native and non-native rainbow trout (P bighead carp> silver carp> native rainbow trout. Local strains of rainbow trout, bighead carp and silver carp exhibited stable IGF-I mRNA levels, at different stages of growth. The imported broodstock of rainbow trout exhibited increased IGF-I levels during the entire period studied, with a peak in the IGF-I mRNA levels of fingerlings. The results indicate intra- and inter-specific variability of IGF-I expression in farmed fish.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"155-162"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49349675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadirah Musa, C-F Low, H. Ramli, M. Manaf, Kok Leong Lee, S. Tan, A. S. Aznan, N. Musa
{"title":"Prominent vulnerability of red hybrid tilapia (Oreochromis spp.) liver to heat stress-induced oxidative damage","authors":"Nadirah Musa, C-F Low, H. Ramli, M. Manaf, Kok Leong Lee, S. Tan, A. S. Aznan, N. Musa","doi":"10.22034/IAR.2021.1924489.1140","DOIUrl":"https://doi.org/10.22034/IAR.2021.1924489.1140","url":null,"abstract":"Many aquatic species are vulnerable to warming water temperatures. Nonetheless, data on the physiological impact of global warming on cultured freshwater fish species are limited compared to marine species. This study investigated the effect of heat stress on oxidative stress response in red hybrid tilapia, Oreochromis spp. (total length 37.0 ± 3.2 cm, body weight 500.0 ± 15.0 g). Fish were assigned randomly and exposed to gradual increment of water temperature (1°C. 8 h-1) from 28 to 31°C in aerated and thermoregulated fibreglass tanks for 2 weeks. Oxidative stress response was determined based on malondialdehyde levels and activities of antioxidant enzymes: superoxide dismutase, catalase and gluthathione s-transferase in the muscle, liver and kidney on day 1, 7 and 14. The heat-stressed group showed significantly increased malondialdehyde levels in the muscle, liver and kidney in parallel to the exposure period. The highest malondialdehyde level was observed in the liver tissue. Activity of superoxide dismutase was significantly decreased in the muscle over the course of the exposure period, in contrast to liver and kidney. Catalase activity was significantly higher in the muscle and liver, while gluthathione s-transferase activity was significantly increased in the muscle and liver but decreased in the kidney. The level of malondialdehyde strongly correlated with superoxide dismutase, catalase and gluthathione s-transferase activities in the liver compared with the muscle and kidney. Microscopic examination of liver showed congestion and hepatocytes with karyorrhexis indicating progress of necrosis. Our results suggest that liver is more susceptible to heat stress-induced oxidative damage compared with muscle and kidney. Red hybrid tilapia showed narrow upper thermal tolerance, implicating high vulnerability to the rise in water temperature.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"109-118"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45443168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental study on the influence of water temperature on the otolith formation of the marbled flounder (Pseudopleuronectes yokohamae)","authors":"Peiqi Hong, Shengjian Jiang, S. Katayama","doi":"10.22034/IAR.2021.1916978.1119","DOIUrl":"https://doi.org/10.22034/IAR.2021.1916978.1119","url":null,"abstract":"The marbled flounder (Pseudopleuronectes yokohamae) is a widely distributed, economically important demersal species in Japan. A study into the growth of marbled flounder in six sampling areas around Japan also found significant differences in otolith nucleus size (diameter of otolith opaque core area) among areas, warm areas showed the bigger nucleus size of otolith, and cold areas showed the smaller size. Based on the physical environmental factors, it was suggested that water temperature affected the nucleus size among these sampling areas. To investigate the factors that influenced the formation of opaque and translucent zones in otolith nucleus, we conducted a rearing experiment using juvenile fish at 122 days post hatch (dph). Fish were reared at salinity of 33 ‰ and different water temperatures (15 ℃, 20 ℃, 25 ℃) from 138 to 213 dph. The otolith growth was correlated positively to water temperature. We also measured the relative greyscale values of the opaque and translucent zones during the period of rearing experiment in each otolith, which showed a negative correlation with water temperature. The results showed that there was a significant relationship between water temperature and otolith formation. The otolith of the marbled flounder will grow larger and have a higher matrix density at higher water temperatures.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"119-128"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46121291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Naiel, M. Elnakeeb, L. Vasilyeva, N. Sudakova, A. Anokhina, A. Gewida, Mahmoud S. Amer
{"title":"Paddlefish, Polyodon spathula: Historical, current status and future aquaculture prospects in Russia","authors":"M. Naiel, M. Elnakeeb, L. Vasilyeva, N. Sudakova, A. Anokhina, A. Gewida, Mahmoud S. Amer","doi":"10.22034/IAR.2021.1920885.1129","DOIUrl":"https://doi.org/10.22034/IAR.2021.1920885.1129","url":null,"abstract":"In the wild, overfishing, habitat loss, industrial and agriculture activity has led to the degradation of freshwater habitats and, therefore, dramatically declines the Mississippi paddlefish (Polyodon spathula) stocks (Stone 2007). Paddlefish gain a growing commercial interest in the USSR/Russian Federation due to its ability to produce high-quality caviar. Also, it can be reared in polyculture ponds and a wide range of temperate reservoirs. Therefore, aquaculture of these valuable fish is necessary to meet consumer demand in Eastern European markets with flesh and caviar. Published information about the culture of paddlefish in the USSR/Russian Federation is scarce and not permanently available. Regarding our study, data were collected from English reports and translated Russian official statistics and studies. Also, FAO fisheries data from 1880 up to 2010 for paddlefish were included. Moreover, caviar production data and prices were surveyed from companies. The collected data could briefly represent the historical and current status of paddlefish aquaculture and provide insight into the expected future production in the Russian Federation. Besides, it investigates the drawbacks of paddlefish aquaculture development and the potential challenges in Russian territories. Also, it discusses potential impacts related to the preservation of the breeding stocks, population naturalization and invasiveness of the species in the southern parts of Russia (i.e., in the Volga‐Caspian basin): Saratov, Rostov, Astrakhan Oblast and Krasnodar regions. Furthermore, this review aimed to gather the available data and the latest final findings that may address the welfare of paddlefish data to fill the knowledge gap towards improving the management of this species. In addition, future development prospects can be supported by government oversight through accurate accounting and financing for private farm activity.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"89-107"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44571460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahbubul Hassan, I. Hoshan, Mst Antara Masuda, I. Parvez, N. W. Rasdi, C. Roy
{"title":"Motility activation and short-term storage of silver carp, Hypopthalmichthys molitrix, sperm","authors":"Mahbubul Hassan, I. Hoshan, Mst Antara Masuda, I. Parvez, N. W. Rasdi, C. Roy","doi":"10.22034/IAR.2021.1921416.1130","DOIUrl":"https://doi.org/10.22034/IAR.2021.1921416.1130","url":null,"abstract":"Sperm activation and short-term storage have important implications in breeding programs. Although silver carp, Hypophthalmicthys molitrix, is an important aquaculture species in South Asia, information on sperm biology and storage is limited. The study aims to test the efficacy of NaCl solutions for activation and short-term storage of silver carp sperm. We found that the motility of silver carp sperm lasted for 45 sec after activation in distilled water. Sperm motility was higher within the first 10 sec of sperm activation, but sperm movement decreased with increasing activation time. When sperm was suspended a graded dilution of NaCl solutions (17 mM to 188 mM), sperm was immobilized at 154mM NaCl (287 mOsmol kg–1). The efficacy of sperm immobilizing solutions was validated during short-term storage at 4°C. A total of 7.1 ± 3.1% sperm was motile in 154 mM NaCl after 5 days of storage. Sperm motility lasted up to 2 days when sperm diluted in 103, 120, 171 and 188 mM NaCl solutions. The osmotic balance between seminal plasma and NaCl solution prepared at 154 mM prevented sperm activation and provided a suitable media for short-term storage. A 154 mM NaCl solution can be used for sperm collection and short-term storage of silver carp sperm.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"139-145"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43739719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation","authors":"Yusuf Bozkurt, İ. Yavaş","doi":"10.22034/IAR.2021.1926852.1151","DOIUrl":"https://doi.org/10.22034/IAR.2021.1926852.1151","url":null,"abstract":"The supplementation of extenders with PUFAs has not been investigated in fish by now. Thus, the present study examined whether different levels of docosahexaenoic (DHA), which is the predominat PUFAs in sperm plasma membrane, in Tris-glucose based extender could increase the quality and fertilizing ability of brown trout (Salmo trutta macrostigma) sperm following cryopreservation were evaluated. Sperm samples diluted (1:10) with the base extender were supplemented with 1, 2.5, 5, 7.5 and 10 ng mL-1 concentrations of DHA. In the control group, sperm was diluted in the base extender, without DHA. Following dilution, the sperm was aspirated into 0.25 mL straws, frozen 3 cm above of the liquid nitrogen (LN2) surface, and plunged directly into the LN2. Based on the results, increasing of DHA supplementations in the Tris-glucose based extender improved post-thaw progressive motility, progressive duration of motility, viability, and fertility compared to the control group (p <0.05). Additionally, the results indicated that supplementation of 7.5 ng mL-1 of DHA significantly exhibited the best cryoprotective effect in terms of progressive motility (62.4±1.73%), progressive duration of motility (46.8±1.32s), viability (56.2±1.20%), fertility (52.6±1.48%), and hatching (28.6±1.45%). The results showed that supplementation of DHA to the Tris-glucose based extender can be beneficial for the cryopreservation of brown trout sperm.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"147-154"},"PeriodicalIF":1.3,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42756830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of dietary tamarind (Tamarindus indica L.) leaves extract on growth performance, nutrient utilization, gut physiology, and susceptibility to Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus L.)","authors":"O. V. Adeniyi, F. Olaifa, B. Emikpe, S. Ogunbanwo","doi":"10.22034/IAR.2021.1916077.1115","DOIUrl":"https://doi.org/10.22034/IAR.2021.1916077.1115","url":null,"abstract":"The rise in global concern on the adverse effects of uncontrolled utilization of synthetic antibiotics in the production of food fish necessitates search for alternative natural products in aquaculture. Tamarind (Tamarindus indica) leaf has great medicinal potentials but with scanty documentation of its utilization in fish production. Therefore, this study investigated the effect of tamarind leaves extract (TLE) on the growth performance, apparent nutrient digestibility, gut physiology, and resistance against Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) fingerlings. The fish were fed experimental diets enriched with 0.0 (control), 5, 10, 15 or 20 g TLE/kg diet at 3% body weight daily for 12 weeks. Thereafter, a 4-week challenge test with A. hydrophila infection was done. The results showed that dietary TLE significantly (P < 0.05) enhanced fish growth, nutrient digestibility, and utilization, villi height and absorption area at 1.0-1.5% inclusion levels, compared to the control diet. Regression analysis showed 1.12% as the level of TLE for optimum weight gain. Post-challenge fish fed TLE-enriched diets showed higher survival rate, relatively to fish fed the control diet. The results from the present study demonstrated that dietary TLE promoted growth, nutrient digestibility and protection against A. hydrophila infection in Nile tilapia and its inclusion at 1.0% was therefore recommended for aquaculture use.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"37-51"},"PeriodicalIF":1.3,"publicationDate":"2021-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42461152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study of steroid hormones, biochemical parameters and body size based on different gonad's quality and gonad's fat content from sonogrphy method in female cultured Siberian sturgeon (Acipenser baerii)","authors":"Aflaton Pourali, M. Sohrabnezhad","doi":"10.22034/IAR.2021.679355","DOIUrl":"https://doi.org/10.22034/IAR.2021.679355","url":null,"abstract":"Farmed female Siberian sturgeon's gonad (n= 30, 3-year old) was examined using an ultrasonograph unit with a 9–13 MHz linear transducer and results were compared with serum steroid hormones, biochemical parameters and body size. The sonogram was practically interpreted based on two indices as gonad's size based on gonad's size and gonadal fat content in the three classifications (small, medium, and large). As evidenced, 73.3% of fishes were considered as weak gonadal fat content; adversely most of which had large gonad's quality. From the results, gonad's quality showed significant correlation with body size, in which larger fishes had medium and large gonad's quality. This ensures the more success of production of females for both caviar production and broodstocks production purposes when larger fishes in the same age are known in a farm and could be separated for better management. Pearson’s correlation showed that there was a significant positive relationship between body length and triglyceride (R = 0.571, P < 0.01) and between body weight and triglyceride (R = 0.665, P < 0.000). Furthermore, a positive trend of triglyceride level in large gonad's quality group rather than weak group could certify it as a potential serum's biomarker to detect larger female accompanied with relatively larger and more productive gonad in Siberian sturgeon.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"13 1","pages":"29-36"},"PeriodicalIF":1.3,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46899064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}