Indian Journal of Applied Microbiology最新文献_第4页

Characterization and Antagonistic Control Measures of Pichia kudriavzevii Yeast like Fungus in Brown Rot Disease of Citrus sinensis (L.) Osbeck 柑桔褐腐病病原菌Pichia kudriavzev样菌的鉴定及拮抗措施等

Indian Journal of Applied Microbiology Pub Date : 2019-01-01 DOI: 10.46798/ijam.2019.v22i01.004

Faruk Hasan, A. Islam, B. Sikdar

{"title":"Characterization and Antagonistic Control Measures of Pichia kudriavzevii Yeast like Fungus in Brown Rot Disease of Citrus sinensis (L.) Osbeck","authors":"Faruk Hasan, A. Islam, B. Sikdar","doi":"10.46798/ijam.2019.v22i01.004","DOIUrl":"https://doi.org/10.46798/ijam.2019.v22i01.004","url":null,"abstract":"Citrus sinensis is one of the most important Vitamin C containing fruit all over the world which is badly infected by brown rot disease (BRD). The present research work was designed to isolate and characterize the pathogenic fungus responsible for BRD of C. sinensis as well as evaluation of its antagonistic control measures. The causal agent of BRD was isolated and characterized using physiological, morphological and molecular methods. The pathogenic fungus was isolated from the skin of sweet orange. The isolated fungus was grown in different nutrients media, temperature, pH, sugar concentrations and different carbohydrates. The highest growth of the fungi was observed in 5% fructose containing PD broth media at 37C, pH 7.5 and 0.05gm/L NaCl concentration. PCR amplification of the isolated fungal DNA by internal transcribed spacer (ITS) region products showed approximately 650bp size of clear cut band. sequencing analysis showed that the amplified its region of the fungus (GenBank Submission# SUB5672073) has 99.80% similarities with the sequences of Pichia kudriavzevii isolate 11-577 its-1 partial sequence. In pathogenicity assay, artificially infected P. kudriavzevii fungus in lemon, malta and apple fruits showed a typical brown rot disease symptoms and the isolates showed similar size of 650bp clear band for PCR products. In antifungal activity assay, the methanol extracts of Allium sativum displayed highest 17.18±0.66 mm diameter zone of inhibition against the isolated fungus. Rhizobium leguminosarum and Alternaria spp. showed significant antagonistic effect in coculture with the isolated fungus. The present research work would be helpful to control the devastating brown rot disease of Citrus sinensis.","PeriodicalId":13518,"journal":{"name":"Indian Journal of Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88666198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremedial Potential of Moderately Halophilic Bacteria 中等嗜盐细菌的生物修复潜力

Indian Journal of Applied Microbiology Pub Date : 2018-12-13 DOI: 10.46798/ijam.2018.v21i03.003

Dr. Reena G. Desai, D. R. Patel

引用次数: 0

Degradation of Profenofos in Soil inoculated with Bacillus cereus and Aneurinibacillus migulanus 蜡样芽孢杆菌和小动脉瘤芽孢杆菌接种土壤对丙烯磷的降解研究

Indian Journal of Applied Microbiology Pub Date : 2018-12-13 DOI: 10.46798/ijam.2018.v21i03.004

Anitha Palanimanickam, Umamaheswari Sepperumal

引用次数: 0

Profile of Dermatophytic infections in Government Tiruvannamalai medical college, Thiruvannamalai 蒂鲁万纳马莱政府医学院皮肤真菌感染概况

Indian Journal of Applied Microbiology Pub Date : 2018-12-13 DOI: 10.46798/ijam.2018.v21i03.002

Sripriya Cs, K. K, V. R, Pandian J, S. K., S. D.

{"title":"Profile of Dermatophytic infections in Government Tiruvannamalai medical college, Thiruvannamalai","authors":"Sripriya Cs, K. K, V. R, Pandian J, S. K., S. D.","doi":"10.46798/ijam.2018.v21i03.002","DOIUrl":"https://doi.org/10.46798/ijam.2018.v21i03.002","url":null,"abstract":"Dermatophyte infections are extremely frequent worldwide and their epidemiological features vary according to the geographical area. Background: This study was undertaken to determine the prevalence of dermatophytosis and the spectrum of ringworm fungi in patients attending a tertiary hospital. Materials & Methods: Samples were collected from 200 patients. A portion of each sample was examined microscopically and the remaining portion of each sample was cultured onto plates of Sabouraud’s dextrose agar containing chloramphenicol with and without cycloheximide. Dermatophyte isolates were identified by studying macroscopic and microscopic characteristics of their colonies. Results: Out of 200 samples, fungi were detected in 148 (74%) by KOH while 158 (79%) samples were culture positive of which 130 isolates were dermatophytes. Among dermatophyte isolates T. rubrum was the most common (36 %) cause of infection. Tinea unguium was the predominant clinical manifestation accounting for 38% of the cases. T. rubrum was the most common pathogen in Tinea unguium and Tinea capitis, whereas T. mentagrophytes was the most common pathogen in tinea pedis. Conclusion: Further intensive epidemiological studies of dermatophytosis which have public health significance are needed.","PeriodicalId":13518,"journal":{"name":"Indian Journal of Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77465468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of potential Tannase producing fungi from Mangroves and Tanneries 红树林和制革厂潜在产丹宁酶真菌的分离与鉴定

Indian Journal of Applied Microbiology Pub Date : 2018-12-13 DOI: 10.46798/ijam.2018.v21i03.001

R. Neethu, N. S. Pradeep

引用次数: 0

Larvicidal Potential of Mycosynthesized Silver Nanoparticles against Culex quinquefasciatus 真菌合成纳米银对致倦库蚊的杀幼虫活性研究

Indian Journal of Applied Microbiology Pub Date : 2018-11-24 DOI: 10.46798/ijam.2018.v21i02.004

P. M., J. P, Prabha T

引用次数: 0

Prevalence and Drug resistance pattern of Klebsiella pneumoniae causing Community - Acquired Pneumonia in Paonta Sahib Region of Himachal Pradesh 喜马偕尔邦Paonta Sahib地区引起社区获得性肺炎的肺炎克雷伯菌患病率和耐药模式

Indian Journal of Applied Microbiology Pub Date : 2018-11-24 DOI: 10.46798/ijam.2018.v21i02.001

Mony Thakur, K. -

引用次数: 2

Comparison of antimicrobial study of extracted essential oil clove and readymade essential oil neem 萃取丁香精油与熟品楝树精油抑菌研究之比较

Indian Journal of Applied Microbiology Pub Date : 2018-11-24 DOI: 10.46798/ijam.2018.v21i02.005

Patel Prachi S, Desai Reena G.

{"title":"Comparison of antimicrobial study of extracted essential oil clove and readymade essential oil neem","authors":"Patel Prachi S, Desai Reena G.","doi":"10.46798/ijam.2018.v21i02.005","DOIUrl":"https://doi.org/10.46798/ijam.2018.v21i02.005","url":null,"abstract":"Essential oils are widely used in the treatment of skin disease. They are also important as a flavouring agent in the food industry. Clove essential oil is used traditionally as flavouring agent and antimicrobial material in food. Neem oil is beneficial to the skin. The study was done on antibacterial and antifungal activities of extracted and readymade essential oils of clove spice and Neem herb. The further study of total phenolic content of essential oils. The essential oils of spice and herb showed antibacterial and antifungal activities which were tested against Gram negative bacteria such as Escherichia coli(ATCC 9961), Proteus vulgaris(ATCC 25933), Salmonella typhi(ATCC 23564), Pseudomonas aeruginosa(ATCC 19154), Gram positive bacteria Staphylococcus aureus(ATCC 6538) and filamentous fungi Aspergillus niger(ATCC 934), Mucor sp.(ATCC 1279), Penicillium sp.(ATCC 28682). Agar well diffusion assay was performed to study the antibacterial and antifungal activities of essential oils. Total phenolic content of essential oils was measured by Folin-Ciocalteu method. Highest antibacterial activity of clove oil observed at 100μl concentration, gave 19 mm zone of inhibition against Pseudomonas aeruginosa. Highest antifungal activity of clove oil observed at 100μl concentration, gave 31 mm against Aspergillus niger and Penicillium sp. And also found that clove essential oil have 23.44 mg/g highest phenolic compound. Phenolic compound is most important compound which is responsible for antibacterial and antifungal activities. The study reported important and comparison of spice and herb essential oils and their used as antibacterial and antifungal agent in pharmaceutical and food industry.","PeriodicalId":13518,"journal":{"name":"Indian Journal of Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90566657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional traits of exopolysaccharide (EPS) producing lactic flora from feces of Irula and Urban community of Tamil Nadu 泰米尔纳德邦Irula和城市社区粪便中产外多糖(EPS)乳酸菌群的功能特征

Indian Journal of Applied Microbiology Pub Date : 2018-11-24 DOI: 10.46798/ijam.2018.v21i02.002

J. Jabastin, P. B. Devi, R. Suresh, V. Priyadarisini

引用次数: 0

A Microbiological Profile of Early Onset of Neonatal Sepsis in A Tertiary Care Hospital in North India 北印度一家三级医院早期新生儿败血症的微生物学概况

Indian Journal of Applied Microbiology Pub Date : 2018-11-24 DOI: 10.46798/ijam.2018.v21i02.003

Y. Bala, V. Randhawa, A. Saili, R. Kaur, S. Chitkara, Aditya Dugga

引用次数: 2