Indonesian Journal of Biotechnology最新文献_第4页

New sources of papain: SEM and SDS‐PAGE analysis to determine the natural tenderizer from papaya latex and senesced leaves 木瓜蛋白酶的新来源:SEM和SDS - PAGE分析从木瓜乳胶和衰老叶片中确定天然嫩化剂

Indonesian Journal of Biotechnology Pub Date : 2022-09-30 DOI: 10.22146/ijbiotech.66434

A. Kartika, H. S. Kusuma, S. Darmawati

{"title":"New sources of papain: SEM and SDS‐PAGE analysis to determine the natural tenderizer from papaya latex and senesced leaves","authors":"A. Kartika, H. S. Kusuma, S. Darmawati","doi":"10.22146/ijbiotech.66434","DOIUrl":"https://doi.org/10.22146/ijbiotech.66434","url":null,"abstract":"This study aims to determine the effectiveness of papaya‐fruit latex and yellow‐senesced leaves as a natural and organic tenderizer. The fruit and leaves of the plant were ground to powder, while 0 g, 10 g, 15 g and 20 g variations were used to cover 50 g of meat for 4 h. Subsequently, the Bradford and Kjeldahl methods were used to determine the protein content, while the protein profile was analyzed using SDS‐PAGE and confirmed using a Scanning Electron Microscope (SEM). The results showed that the protein concentration in mutton after fruit latex treatment was 41%, which was higher than the concentration of beef at 29.86%. Furthermore, the beef lost protein bands and its molecular weight fell from 225 kDa to 86 KDa, while the mutton experienced a reduction from 100 kDa to 65 kDa, which was significantly smaller than for raw meat. A single protein band was also observed at 21.6 kDa in the sample, indicating the presence of papain enzyme protein. Meanwhile, the SEM results showed that collagen and myofibril in the muscles were damaged in the treated meats. Based on these results, treatment with papaya fruit latex and yellow papaya leaves increases the tenderness of meat.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48974335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effectivity of thidiazuron and 1‐naphthaleneacetic acid on somatic embryo induction in transgenic Dendrobium phalaenopsis Fitzg. carrying 35S::GR::AtRKD4 噻二唑仑和1-萘乙酸对转基因蝴蝶兰体细胞胚诱导的影响。携带35S:：GR:：AtRKD4

Indonesian Journal of Biotechnology Pub Date : 2022-09-30 DOI: 10.22146/ijbiotech.70833

M. Ilham, F. Puspitasari, E. Semiarti

{"title":"The effectivity of thidiazuron and 1‐naphthaleneacetic acid on somatic embryo induction in transgenic Dendrobium phalaenopsis Fitzg. carrying 35S::GR::AtRKD4","authors":"M. Ilham, F. Puspitasari, E. Semiarti","doi":"10.22146/ijbiotech.70833","DOIUrl":"https://doi.org/10.22146/ijbiotech.70833","url":null,"abstract":"Dendrobium phalaenopsis Fitzg. (also known as the Larat orchid) is an endemic orchid from Larat Island, Eastern Indonesia. Its beautiful flowers mean that many plants are taken for commercial purposes, leading to the rapid decline of populations in their natural habitats. The objectives of this study were to determine which organs of the transgenic Larat orchid carrying the 35S::GR::AtRKD4 construct, together with which concentrations of the plant growth regulators (PGRs) auxin and cytokinin, are suitable for the induction of somatic embryos (SEs). In this study, the AtRKD4 gene in Larat orchids was confirmed using PCR with specific primers for the AtRKD4 and HPT genes. Thidiazuron (TDZ) (1, 3 and 5 mg/L) in combination with 1‐naphthaleneacetic acid (NAA) (0.5 and 1 mg/L) were used on new phalaenopsis (NP) medium to induce SEs from leaves, pseudobulbs and roots. The AtRKD4 transgenes were detected as being stably integrated into the DNA genome of transformant plants using specific primers for AtRKD4 and HPT genes, and positive results were obtained using actin gene primers as internal controls for PCR. Pseudobulbs produced 19 to 20 SEs from 108 pseudobulb explants (89–100%), a higher number than produced in explants of the other organs studied. Among the PGR treatments, the best results were obtained in NP medium supplemented with a combination of 1 mg/L TDZ and 1 mg/L NAA, 100% of the explants of which produced SEs (2.11 ± 1.36). No significant difference was found between the morphology of the SEs produced from the non‐transformant Larat orchid pseudobulb explants and the 35S::AtRKD4 carrier transformant.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45712041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island 海洋海绵Jaspis sp.相关细菌作为Enggano岛抗菌生产商的潜力

Indonesian Journal of Biotechnology Pub Date : 2022-09-30 DOI: 10.22146/ijbiotech.65943

S. Sipriyadi, Riziq Ilham Nurfahmi, U. Cahlia, R. H. Wibowo, W. Darwis, E. Nugraheni

{"title":"Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island","authors":"S. Sipriyadi, Riziq Ilham Nurfahmi, U. Cahlia, R. H. Wibowo, W. Darwis, E. Nugraheni","doi":"10.22146/ijbiotech.65943","DOIUrl":"https://doi.org/10.22146/ijbiotech.65943","url":null,"abstract":"Sponges, a group of marine multicellular animals with a porous body structure, show potential for the production of bioactive compounds. Sponge‐associated bacteria are an alternative antimicrobial producer due to their high content of bioactive compounds. This study aimed to identify the highest‐potential antimicrobial‐producing bacteria isolate associated with Jaspis sp. sponges from Enggano island. The isolated bacteria were screened for antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans using cultures, supernatants, pellets, and crude extracts. The study also conducted genetic identification to determine the identity of the isolate with the greatest potency and its closest relationship using the 16S rRNA gene. The antimicrobial activity was determined by monitoring and measuring the diameter of the formed clear zones. The results of the observations of morphological characteristics revealed nine isolates from Jaspis sp. that each consisting of 6 JABS isolates and 3 JABB isolates. Based on isolates that had antimicrobial activity, JABS6 isolates had the best antimicrobial activity, with the diameter of inhibition zones of 24.7, 8.2, 4.6, and 33.7 mm for E. coli, P. aeruginosa, S. aureus and C. albicans, respectively. The genome sequencing of the JABS6 isolate confirmed that it was identical to Bacillus thuringiensis strain USS‐CAP‐1. The study concludes that this finding shows promise for the further development of future antimicrobial agents.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44301690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast 卡托普利联合5-氟尿嘧啶对瘢痕疙瘩成纤维细胞增殖及胶原沉积的影响

Indonesian Journal of Biotechnology Pub Date : 2022-09-30 DOI: 10.22146/ijbiotech.69505

Jesslyn Amelia, Y. Wirohadidjojo, Agnes Sri Siswati

{"title":"The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast","authors":"Jesslyn Amelia, Y. Wirohadidjojo, Agnes Sri Siswati","doi":"10.22146/ijbiotech.69505","DOIUrl":"https://doi.org/10.22146/ijbiotech.69505","url":null,"abstract":"Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5‐fluorouracil (5‐FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5‐FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4–7. The study consisted of a control group, captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L), 5‐FU 1 mg/mL and a combination of captopril at various concentrations with 5‐FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L) and the 5‐FU group against the control group (p<0.05). In the combined‐dose group, captopril at a concentration of 10‐2 mol/L and 5‐FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5‐FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10‐2 mol/L and 5‐FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5‐FU as a single therapeutic agent.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44049551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample 基于唾液样本核衣壳检测的自给药COVID - 19快速检测方法的早期开发

Indonesian Journal of Biotechnology Pub Date : 2022-09-30 DOI: 10.22146/ijbiotech.72269

S. Soidah, T. Subroto, Sari Syahruni, Fauzian Giansyah, Henry Chandra, D. Salsabila, B. Alisjahbana, N. Fauziah, H. L. Wiraswati, Leonardus Wiydatmoko, B. Andriyoko, Anita Yuwita, Muhammad Yusuf

{"title":"Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample","authors":"S. Soidah, T. Subroto, Sari Syahruni, Fauzian Giansyah, Henry Chandra, D. Salsabila, B. Alisjahbana, N. Fauziah, H. L. Wiraswati, Leonardus Wiydatmoko, B. Andriyoko, Anita Yuwita, Muhammad Yusuf","doi":"10.22146/ijbiotech.72269","DOIUrl":"https://doi.org/10.22146/ijbiotech.72269","url":null,"abstract":"More than 6,000,000 people have died due to the coronavirus (COVID‐19) pandemic. This disease spread quickly due to its highly contagious nature. The SARS‐CoV‐2 virus that causes the disease can be transmitted through saliva droplets secreted by infected people at a distance of less than 1 m. As a result, saliva has been accepted as an alternative specimen for COVID‐19 detection by the Centers for Disease Control and Prevention (CDC). Furthermore, WHO recommended the use of rapid antigen tests based on lateral flow immunoassay when reverse transcription‐polymerase chain reaction (RT‐PCR) is not available. We developed a saliva‐based rapid antigen test by optimizing the antibody concentration and optimum pH for the conjugation of antibody and gold nanoparticles. We found that the best running buffer formulation consisted of 75 mM sodium phosphate buffer, 1% NaCl, 1% Triton X‐100, 0.5% N‐acetyl‐L‐cysteine, and 0.02% sodium azide. The addition of a mucolytic agent in the buffer can reduce the viscosity of saliva, thus improving sensitivity. The rapid test developed detected the lowest concentration of nucleocapsid protein at 0.1 μg/mL. Our study revealed 100% specificity against negative COVID‐19 saliva and no cross‐reaction with avian influenza virus hemagglutinin.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42625889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti‐proliferative effects of pentagamaboronon‐0‐sorbitol on HER2‐overexpressing breast cancer cells 山梨醇对过表达HER2的乳腺癌细胞的抗增殖作用

Indonesian Journal of Biotechnology Pub Date : 2022-06-29 DOI: 10.22146/ijbiotech.67549

Lailatul Qodria, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto

{"title":"Anti‐proliferative effects of pentagamaboronon‐0‐sorbitol on HER2‐overexpressing breast cancer cells","authors":"Lailatul Qodria, Rohmad Yudi Utomo, A. Hermawan, E. Meiyanto","doi":"10.22146/ijbiotech.67549","DOIUrl":"https://doi.org/10.22146/ijbiotech.67549","url":null,"abstract":"HER2‐positive breast cancer is an aggressive form of the disease that is associated with poor prognosis and chemo‐resistance. As such, investigation continues into the development of a new HER2‐targeted drug for breast cancer. This study investigated the anti‐proliferative activities of pentagamaboronon‐0‐sorbitol (PGB‐0‐So) in HER2‐overexpressing breast cancer (MCF‐7/HER2) cells. The cytotoxicity of PGB‐0‐So was assessed via MTT assay. Flow cytometry with propidium iodide and annexin‐V‐FITC staining was conducted to investigate the mechanism of PGB‐0‐So in inhibiting the proliferation of MCF‐7/HER2 cells. Finally, FACS analysis with 2′,7′–dichlorofluorescin diacetate staining was performed to examine intracellular ROS production. PGB‐0‐So exerted cytotoxicity towards MCF‐7/HER2 breast cancer cells with an IC50 value of 36 μM. PGB‐0‐So induced S‐phase arrest and apoptosis in MCF‐7/HER2 cells. Moreover, PGB‐0‐So could increase intracellular ROS production in MCF‐7/HER2 cells. PGB‐0‐So exerted anti‐proliferative activity towards MCF‐7/HER2 cells. This compound may be developed as a chemotherapeutic agent against HER2‐overexpressing breast cancer.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46200690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Network pharmacology of black cumin (Nigella sativa L.) as a candidate of OMAI in colorectal cancer: in silico study 黑孜然(Nigella sativa L.)作为大肠癌OMAI候选药物的网络药理学:计算机研究

Indonesian Journal of Biotechnology Pub Date : 2022-06-29 DOI: 10.22146/ijbiotech.70699

Firzannida Firzannida, Sakti Bagaskara, Savana Sonia Savira, Aufa Fadnurrahim, Siti Rofida

{"title":"Network pharmacology of black cumin (Nigella sativa L.) as a candidate of OMAI in colorectal cancer: in silico study","authors":"Firzannida Firzannida, Sakti Bagaskara, Savana Sonia Savira, Aufa Fadnurrahim, Siti Rofida","doi":"10.22146/ijbiotech.70699","DOIUrl":"https://doi.org/10.22146/ijbiotech.70699","url":null,"abstract":"Colorectal cancer is the third most common cancer globally and the second leading cause of cancer‐related deaths. The management of colorectal cancer requires consideration of various factors due to the non‐selectivity of drugs, meaning that highly effective treatment with lower side effects is needed. Black cumin (Nigella sativa L.) contains thymoquinone and various other metabolites with potential as anticancer effects. The involvement of various genes and the difficulty of drug development have led to a ashift in the drug development paradigm towards plant‐based medicine that is both multicomponent and synergistic in supporting the resulting pharmacological effects. Network pharmacology can predict the synergistic effect of a multicomponent approach. This study aimed to predict the network pharmacology of black cumin as a candidate for OMAI (“Obat Modern Asli Indonesia”, Indonesian‐origin modern medicine) in colorectal cancer. This research was an in silico study using various ethnobotanical databases and software. The results show that seven metabolites in black cumin are correlated with ten surface receptor proteins, 30 intracellular proteins, and mechanisms involving six colorectal cancer signaling pathways. This result indicates that Nigella sativa L. has potential in OMAI and can be a reference for the development of cancer treatment, especially for colorectal cancer.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47347448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of different parameters on cellulase production by Trichoderma harzianum TF2 using solid‐state fermentation (SSF) 不同参数对哈茨木霉TF2固态发酵产纤维素酶的影响

Indonesian Journal of Biotechnology Pub Date : 2022-06-29 DOI: 10.22146/ijbiotech.66549

J. Heng, Haliza Hamzah

引用次数: 1

Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction parijoto（Medinilla speciosa Reinw.Ex.Bl.）甲醇提取物与顺铂联合诱导WiDr结肠癌癌症细胞凋亡的细胞毒性作用

Indonesian Journal of Biotechnology Pub Date : 2022-06-29 DOI: 10.22146/ijbiotech.63766

A. N. Artanti, F. Prihapsara, Ranita Kumalasari Susanto

{"title":"Cytotoxic effects of parijoto (Medinilla speciosa Reinw. Ex. Bl.) methanol extract combined with cisplatin on WiDr colon cancer cells through apoptosis induction","authors":"A. N. Artanti, F. Prihapsara, Ranita Kumalasari Susanto","doi":"10.22146/ijbiotech.63766","DOIUrl":"https://doi.org/10.22146/ijbiotech.63766","url":null,"abstract":"Parijoto (Medinilla speciosa Reinw. Ex. Bl.) is a medicinal plant with cytotoxic effects on cancer cells in vitro. As only a limited number of studies have reported the effect of parijoto on colon cancer cells, this study initially aimed to measure the total flavonoid levels and potential cytotoxic effects of parijoto methanol extract (PME) through cell viability assays and expression of the apoptotic protein on WiDr colon cancer cells as a model. PME cytotoxic activity was determined by conducting a cytotoxicity test on WiDr colon cancer cells using the MTT assay. The synergistic cytotoxic effects of the PME and cisplatin were tested to obtain the combination index (CI) value. Apoptosis was analyzed by flow cytometry, and the apoptotic protein expression was observed by immunocytochemical tests. Furthermore, quercetin as a major flavonoid in PME was measured using a UV–Vis spectrophotometer. The results showed that PME had a moderate cytotoxic activity with an IC50 of 198.64±1.6 µg/mL, whereas the IC50 of cisplatin was 2.34±0.7 µg/mL. The PME with cisplatin combination test showed a strong synergistic effect with a CI value of <1 (0.1‐0.4). The combination showed increased apoptosis properties compared to PME treatment alone. In addition, immunocytochemistry showed that PME alone or in combination with cisplatin increased the pro‐apoptosis proteins (p53 and caspase‐9) and suppressed Bcl‐2 expression. Moreover, the cell viability value increased as the PME concentration decreased. The administration of PME led to changes in cell morphology, lower cell density, and a decreasing number of living cells. Therefore, the combination of PME and cisplatin had a strong synergistic effect in inducing apoptosis.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44306301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of cytokinin responsive and ethylene biosynthesis genes in rice callus with different regeneration rates 细胞分裂素响应基因和乙烯合成基因在不同再生速率水稻愈伤组织中的表达

Indonesian Journal of Biotechnology Pub Date : 2022-06-29 DOI: 10.22146/ijbiotech.70264

Syafira Fatihatul Husna, P. Dewanti, B. Sugiharto, Wahyu Indra Duwi Fanata

{"title":"Expression of cytokinin responsive and ethylene biosynthesis genes in rice callus with different regeneration rates","authors":"Syafira Fatihatul Husna, P. Dewanti, B. Sugiharto, Wahyu Indra Duwi Fanata","doi":"10.22146/ijbiotech.70264","DOIUrl":"https://doi.org/10.22146/ijbiotech.70264","url":null,"abstract":"This study aimed to investigate the correlation between callus regeneration rate and the expression of several genes responsible for cytokinin response and ethylene biosynthesis in the Ciherang, Mentik Wangi Susu, Hwayoung and Tarabas rice varieties. The callus regeneration rate of each rice variety was in vitro tested using N6 media, while the gene expression during the callus regeneration stages was examined using quantitative real‐time PCR (qRT‐PCR). Our results showed that the callus of Ciherang and Mentik Wangi Susu showed earlier green spot formation that then turned brown at a later stage, resulting in a low regeneration rate. While Hwayoung and Tarabas showed late green spot formation, high shoot regeneration was observed in both calluses. Gene expression analysis of regeneration media‐grown calluses showed that two cytokinin‐responsive genes, OsRR2 and OsRR6, were highly expressed in the Ciherang and Hwayoung callus, respectively. We also observed that ethylene biosynthesis genes such as OsACS1 and OsACO1 were highly expressed in the Mentik Wangi Susu and Hwayoung callus, respectively. Moreover, the expression of OsBBM1 was high in Hwayoung and Tarabas. Thus, the positive correlation between the expression of cytokinin‐responsive and ethylene biosynthesis genes with somatic embryogenesis activity likely depends on the induction level of OsBBM1.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43678398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0