Indonesian Journal of Biotechnology最新文献_第2页

Antibacterial Activity of Bioactive Compound Produces by Endophtic Fungi Isolated from Mangifera casturi Kosterm Endemic Plant from South Kalimantan, Indonesia 南加里曼丹芒果特有植物内生真菌产生物活性化合物的抗菌活性研究

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.71150

Fatia Rizki Nuraini

{"title":"Antibacterial Activity of Bioactive Compound Produces by Endophtic Fungi Isolated from Mangifera casturi Kosterm Endemic Plant from South Kalimantan, Indonesia","authors":"Fatia Rizki Nuraini","doi":"10.22146/ijbiotech.71150","DOIUrl":"https://doi.org/10.22146/ijbiotech.71150","url":null,"abstract":"One of promising bio-prospect as producer of an antibacterial compound is endophytic fungi that live in endemic plants. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi, an South Kalimantan endemic plant that has ethnobotanical utilisation in the pharmaceutical field. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disc diffusion assay. The minimum inhibitory concentration (MIC) was measured by the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage due to the effect of the extract. The antibacterial compounds then were detected using GC-MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequence. Among 13 isolates, endophytic fungi identified as Botryosphaeria rhodina AK32 able to produce antibacterial compounds that exhibited the highest activity and have a broad spectrum, moreover capable against resistant bacteria (MRSA) with 1.56% of MIC value for all of the test bacteria. AK32 ethyl acetate extract was inhibiting the cell wall synthesis and penetrate the outer membrane of bacteria. Based on GC-MS, antibacterial compounds of AK32 ethyl acetate extract were di-n-octylphthalate, phenol, 2-methyl-, 4-pentadecyne, 15-chloro-, benzeneacetonitrile, and benztriazole.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44767357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manufacturing of an effective, eco-friendly nano insecticide with aiding of green alga Chlorella vulgaris in contrast with the traditional insecticide. 与传统杀虫剂相比，利用普通小球藻制备一种有效、环保的纳米杀虫剂。

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.79105

M. Shafeeq

{"title":"Manufacturing of an effective, eco-friendly nano insecticide with aiding of green alga Chlorella vulgaris in contrast with the traditional insecticide.","authors":"M. Shafeeq","doi":"10.22146/ijbiotech.79105","DOIUrl":"https://doi.org/10.22146/ijbiotech.79105","url":null,"abstract":"This study aims to establish an aqueous extract of Chlorella vulgaris as a green factory to manufacture titanium nanoparticles and to apply these nanoparticles to control the house flies (Musca domestica). Since the crystallite size of prepared titanium nanoparticles was 27.39 nm. Furthermore, the observed size for bulk particles ranged (from 92.33-249.6) nm through SEM. While for nanocrystalline the size ranged (from 9.395- 206) nm. Several phytochemicals were detected within algal extracts such as phenols, tannins, alkaloids, flavonoids, resins, and saponins. All these active compounds participated in nano-synthesis by acting as reducing and stabilizing agents. finally, titanium nanoparticles were used as a controlling agent against house flies (Musca domestica) and compared with traditional insecticides (Imidacloprid). High mortality percentages reached 100% against 1st larval stage, 70% against 3rd larval stage, and 93.3% in adult flies. These fatalities were higher after using Imidacloprid for all tested stages. Many phenotypic distortions were also caught in houseflies treated with TiO2 NPs prepared by Chlorella, such as; failure in pupal emergence and maturity, incomplete development in the head, legs, and wings, and disappearance in genital organs. It has shown that Chlorella Vulgaris is a good candidate for nanomanufacturing and a rich naturally derived Nanopesticide.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41951588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker 利用SRAP标记进行种间杂交的匙兰(Dendrobium spp.)杂种的遗传变异和基因组构成

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.83595

A. Purwantoro, A. Setiawan, Rikcy Setiaji Nugraha, Sairoh Bisirotil Mujtaba, A. H. Setyadi

{"title":"Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker","authors":"A. Purwantoro, A. Setiawan, Rikcy Setiaji Nugraha, Sairoh Bisirotil Mujtaba, A. H. Setyadi","doi":"10.22146/ijbiotech.83595","DOIUrl":"https://doi.org/10.22146/ijbiotech.83595","url":null,"abstract":"Dendrobium is the second largest genus in the Orchidaceae family. Dendrobium section Spatulata is widely cultivated in Indonesia due to its ease of cultivation, high economic value and adaptability, and extended flower shelf life. In this study, we have conducted a breeding program for developing new cultivar of Dendrobium section Spatulata through interspecific hybridization. This study is aimed to investigate the genetic variation and genomic constitution of the eight hybrids and their corresponding parental lines resulted from interspecific hybridization using SRAP markers. Dendrobium section Spatulata hybrids produced by interspecific hybridization are genuine hybrids with substantial genetic variability based on flower morphology, including labellum shapes and color intensities, as well as curly horn shapes and color intensities. The SRAP marker, which was utilized to genotype the hybrid and parental lines, exhibited a significant degree of polymorphism, and may be used to distinguish each accession. The UPGMA dendrogram and PCoA biplot showed that all the hybrids were grouped with their corresponding parental lines based on their genetic background and genomic constitution. These findings are critical for genetic improvement of the Spatulata orchid to develop novel varieties.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43122674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture 一种新型中试生物反应器用于平菇不定根的大规模培养

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.78420

Y. Manuhara, D. Y. Kusuma, A. Kristanti

{"title":"Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture","authors":"Y. Manuhara, D. Y. Kusuma, A. Kristanti","doi":"10.22146/ijbiotech.78420","DOIUrl":"https://doi.org/10.22146/ijbiotech.78420","url":null,"abstract":"Adventitious root culture and the development of a bioreactor are methods to obtain biomass and plant bioactive compounds in large quantities faster than conventional plant cultivation. These technologies provide an excellent opportunity to produce biomass and bioactive compounds from plants, especially Gynura procumbens. Previous reports mentioned a small-scale bioreactor could increase biomass and bioactive compounds of G. procumbens adventitious roots. Therefore, a larger bioreactor for adventitious root culture is necessary to develop. In this study, the development of the 19 L pilot bioreactor was successful. The optimal condition for bioreactor sterilization is 1.8 bar for 60 min using an autoclave. We found that G. procumbens adventitious roots culture in pilot bioreactors has resulted in optimal biomass using MS-Tek media (technical grade) compared to MS-PA (pro analyze) media after 35 d of the culture period. Although, higher productivity of total phenolics and total flavonoids in G. procumbens roots has been achieved from MS-PA media rather than MS-Tek media. In further study, it is necessary to evaluate the effect of technical-grade chemicals on kinetics root growth and chemical uptake. The hope is to obtain a suitable media formulation with affordable cost for adventitious root culture production.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47741451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nephroprotective Effects of Cardamom Essential Oil (Amomum compactum Soland. Ex Maton) in Kidney Cells 豆蔻精油对肾细胞的保护作用

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.79642

Nisa Ul Hasanah, Ahmad Syauqy Tafrihani, Ummi Maryam Zulfin, D. Salsabila, Ratih Kurnia Wardani, Muthi’ Ikawati, E. Meiyanto, R. Jenie

{"title":"Nephroprotective Effects of Cardamom Essential Oil (Amomum compactum Soland. Ex Maton) in Kidney Cells","authors":"Nisa Ul Hasanah, Ahmad Syauqy Tafrihani, Ummi Maryam Zulfin, D. Salsabila, Ratih Kurnia Wardani, Muthi’ Ikawati, E. Meiyanto, R. Jenie","doi":"10.22146/ijbiotech.79642","DOIUrl":"https://doi.org/10.22146/ijbiotech.79642","url":null,"abstract":"Many chemotherapeutic agents cause various side effects, including nephrotoxicity. Doxorubicin, for example, increases the level of reactive oxygen species (ROS), leading to cell senescence in the kidneys. Cardamom essential oil (Amomum compactum Soland. ex Maton) contains compounds that exhibit antioxidant activity, such as 1.8-cineole, α-pinene, α-terpineol, and linalool. This study focused on exploring the potency of cardamom essential oil (CEO) as an anti-senescence induced by doxorubicin using Vero cells. CEO was obtained by steam distillation. The cytotoxic assay was carried out using trypan blue exclusion assay. We performed the 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) staining and the senescence-associated beta-galactosidase (SA-β-gal) staining to measure the effect of CEO on intracellular ROS level and cell senescence, respectively. Analysis of the compounds with gas chromatography-mass spectrophotometry (GC-MS), revealed seven compounds with significant abundance, namely 1.8-cineole (50.82%), ß-pinene (12.43%), α-terpineol (8.50%), fenchone (4.10%), α-pinene (4.00%), sabinene (3.00%), and linalool (1.98%). The cytotoxicity assay of CEO on Vero cells showed an IC50 value of 178 μg/mL. Thus, the CEO is considered to be not cytotoxic and safe for normal kidney cells. Concentrations of 50 and 100 μg/mL reduced the senescence induced by doxorubicin. Therefore, the CEO has the potency as a nephroprotective agent in doxorubicin-induced senescence. ","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48985604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of protease activity and anti‐quorum sensing of the potential fraction of ethanolic extract from Sansevieria trifasciata Prain leaves against Pseudomonas aeruginosa 三叶紫薇叶乙醇提取物对铜绿假单胞菌蛋白酶活性的抑制和潜在组分的抗群体感应

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.73649

W. Dewatisari, L. Nugroho, E. Retnaningrum, Y. A. Purwestri

{"title":"Inhibition of protease activity and anti‐quorum sensing of the potential fraction of ethanolic extract from Sansevieria trifasciata Prain leaves against Pseudomonas aeruginosa","authors":"W. Dewatisari, L. Nugroho, E. Retnaningrum, Y. A. Purwestri","doi":"10.22146/ijbiotech.73649","DOIUrl":"https://doi.org/10.22146/ijbiotech.73649","url":null,"abstract":"Sansevieria trifasciata is a plant that is commonly utilized in traditional medicine. The leaves of S. trifasciata show antibacterial properties against Pseudomonas aeruginosa. This bacterium is an opportunistic pathogen that can cause serious illness in humans and produce a variety of virulence factors responsible for bacterial pathogenesis with quorum sensing (QS) systems that mediate intracellular communication. Bacteria produce protease through a QS mechanism in which they express signaling molecules to become pathogens. Proteases are extracellular enzymes required for successful infection that mediate biofilm spread through QS and regulate a variety of cellular and physiological functions. This research aimed to evaluate the protease, and anti‐QS activities of the ethanolic extract from S. trifasciata leaves against P. aeruginosa and the expression of QS genes. An azocasein test was used to determine the protease activity in qualitative and quantitative methods. Using real‐time quantitative polymerase chain reaction, a study was conducted to investigate the effect of ethanolic extract from S. trifasciata leaves on selected QS‐regulatory genes at the transcriptional level. The results showed that the potential ethanolic extract from S. trifasciata leaves inhibited the protease enzyme activity by as much as 77.1%. The potential ethanolic extract from S. trifasciata leaves decreased the expressions of lasA, lasB, lasI, lasR, rhlI, and rhlR with 2‐ΔΔCt values of 0.81, 0.93, 0.76, 0.97, 0.90, and 0.55 respectively.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48950765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Production, purification and characterization of chitinase from Micromonospora sp. AR17 小单孢菌AR17几丁质酶的制备、纯化及特性研究

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.77137

{"title":"Production, purification and characterization of chitinase from Micromonospora sp. AR17","authors":"","doi":"10.22146/ijbiotech.77137","DOIUrl":"https://doi.org/10.22146/ijbiotech.77137","url":null,"abstract":"N‐acetylglucosamine (NAG) is the monomer product of chitin, which has been widely used as a bioactive com‐ pound in applications such as anti‐tumor, anti‐microbial, and antioxidant activities. In production, biological processes using enzymes are preferable to chemicals due to environmental issues. This study aims to determine the activity, purity level, and molecular weight of purified chitinase from Micromonospora sp. AR17 determines the concentration of NAG produced by purified chitinase that has been characterized. Chitinase was produced by fermentation in colloidal chitin broth at 40 °C, pH 7, for 7 days, while chitinase activity was checked every 24 h. The optimal fermentation time was used to produce chitinase for a further purification step. Enzyme purification was carried out by ultrafiltration, ammonium sulfate precipitation, ion exchange chromatography (Q Sepharose Fast Flow), and gel filtration (Sephacryl S‐300). The purified enzyme was then char‐ acterized for optimum time, pH, and temperature to produce NAG. The results suggested that the fourth day was the optimal time for chitinase production, with chitinase activity of 0.0040 U/mL and a NAG concentration of 7.62 µg/mL. The purifica‐ tion step successfully increased the purity by 6.82 times with chitinase‐specific activity at 1.4648 U/mg. Production of NAG with purified chitinase produced a NAG concentration of 32.472 µg/mL with an incubation time of 30 min at 40 °C and pH 7.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43292836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical characterization of folded state type II luciferase‐like monooxygenase 折叠态II型荧光素酶样单加氧酶的生物物理特性

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.75783

Adinda Fitri Salsabila, Abidah Tauchid, M. S. Rohman, D. Widianto, S. Margino

{"title":"Biophysical characterization of folded state type II luciferase‐like monooxygenase","authors":"Adinda Fitri Salsabila, Abidah Tauchid, M. S. Rohman, D. Widianto, S. Margino","doi":"10.22146/ijbiotech.75783","DOIUrl":"https://doi.org/10.22146/ijbiotech.75783","url":null,"abstract":"We noticed that the Priestia megaterium genome contains five Luciferase‐like monooxygenase (LLM) encoding genes, however, their functions are unknown. The objective of this work was to characterize the biophysical properties of the recombinant LLM2 from Priestia megaterium PSA10 through in vitro and in silico approaches. We successfully cloned into the pET vector system and expressed the recombinant LLM2 in Escherichia coli BL21(DE3). The recombinant LLM2 was overproduced and purified in the form of an inclusion body with a molecular weight of ±39.5 kDa when it was analyzed in 15% SDS‐PAGE. The inclusion body of recombinant LLM2 was then refolded and characterized for its biophysical properties by measuring the UV spectrum of 200 to 250 nm wavelength and determining the change of enthalpy (ΔH) and entropy (ΔS) at the melting temperature. The refolded recombinant LLM2 exhibited a strong spectrum at 205 nm, while the unfolded recombinant LLM2 did not. The Tm, ΔHTm, and ΔSTm values of the refolded recombinant LLM2 were determined to be 318.31±4.4 K, 11.76±1.3 kJ.mol‐1, and (3.74±0.48)x10‐2 kJ.mol‐1.K‐1, respectively. The predicted 3D structure of LLM2 showed that the protein contains the TIM‐barrel, resembling the common global fold of bacterial luciferases. Determination of the cofactor preference suggested that the LLM2 preferred FAD for its cofactor.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46277651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing disease‐specific endogenous target mimic of microRNA from long non‐coding RNA identification and characterization in Musa spp. 从穆萨属的长非编码RNA鉴定和表征中揭示微小RNA的疾病特异性内源性靶向模拟物。

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.49368

Audie Masola Putra, H. Nugrahapraja, F. Dwivany

{"title":"Revealing disease‐specific endogenous target mimic of microRNA from long non‐coding RNA identification and characterization in Musa spp.","authors":"Audie Masola Putra, H. Nugrahapraja, F. Dwivany","doi":"10.22146/ijbiotech.49368","DOIUrl":"https://doi.org/10.22146/ijbiotech.49368","url":null,"abstract":"Banana (Musa spp.) is one of the most widely consumed fruits in the world. Unfortunately, the plants are at risk from many disease problems, which mainly derive from microorganism. It is a little known about the relationship between disease‐inducing microorganisms and plants, particularly at the molecular level. This research aimed to characterize long non‐coding RNA (lncRNA) from bananas that may have roles in regulating gene expression related to the disease response mechanism in banana derived from transcriptomic libraries. Furthermore, the detected transcripts were analyzed to identify the endogenous target mimics (eTMs) interaction between lncRNA and microRNA (miRNA) using computational approaches. Data from Cavendish banana (AAA group), Berangan (AAA group), Yunnan Banana (Itinerans), Dajiao (ABB group), and Klutuk (BB group) were used in this research. We found that lncRNA tends to be unsustainable, and most sizes are below 1000 bp (≥ 75%). Based on this result, we investigated the eTMs to determine lncRNA transcripts and miRNA, such as miR397 in Cavendish and miR444 in Klutuk. This transcript would be regulated following exposure to extreme temperatures and disease, indicating the possibility of disease‐specific interaction between bananas and their environment at the molecular level.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42412914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the patchouli essential oil (Pogostemon cablin Benth.) aromatic characteristic by near‐infrared spectroscopy 广藿香精油（Pogostemon cablin Benth.）芳香特性的近红外光谱评价

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.69073

Diego Mauricio Cano-Reinoso, Y. Purwanto, I. Budiastra, S. Kuroki, S. Sutrisno, S. Widodo

{"title":"Evaluation of the patchouli essential oil (Pogostemon cablin Benth.) aromatic characteristic by near‐infrared spectroscopy","authors":"Diego Mauricio Cano-Reinoso, Y. Purwanto, I. Budiastra, S. Kuroki, S. Sutrisno, S. Widodo","doi":"10.22146/ijbiotech.69073","DOIUrl":"https://doi.org/10.22146/ijbiotech.69073","url":null,"abstract":"This study aimed to evaluate the aromatic characteristic of patchouli essential oil (Pogostemon cablin Benth.) by near‐infrared spectroscopy combined with chemometric treatments. The study used 84 oil samples collected from around Indonesia, namely in Konawe, Kolaka, Bogor, Garut, Aceh, Jambi, and Masamba. Several pretreatments were used to process the spectral data, together with the application of partial least squares. The spectrum wavelength applied was between 1000 and 2500 nm. The spectra data were separated to develop two models based on their physical and chemical properties (Bogor, Garut, Konawe, and Kolaka in the first model; Aceh, Jambi, and Masamba in the second one). Liquid chromatography‐mass spectrometry (LC‐MS) was used as a reference method. Patchouli alcohol was established as the main chemical compound of this aromatic oil. The best calibration for the first model was that with mean center normalization as a data pretreatment, while for the second model, it was the one using the second derivative. Both models had a correlation coefficient higher than 0.90 and a coefficient of variation lower than 2.98%. In conclusion, near‐infrared spectroscopy can be employed as an accurate tool to determine the characteristic of patchouli oil.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47150137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0