Indonesian Journal of Biotechnology最新文献_第2页

Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture 一种新型中试生物反应器用于平菇不定根的大规模培养

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.78420

Y. Manuhara, D. Y. Kusuma, A. Kristanti

{"title":"Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture","authors":"Y. Manuhara, D. Y. Kusuma, A. Kristanti","doi":"10.22146/ijbiotech.78420","DOIUrl":"https://doi.org/10.22146/ijbiotech.78420","url":null,"abstract":"Adventitious root culture and the development of a bioreactor are methods to obtain biomass and plant bioactive compounds in large quantities faster than conventional plant cultivation. These technologies provide an excellent opportunity to produce biomass and bioactive compounds from plants, especially Gynura procumbens. Previous reports mentioned a small-scale bioreactor could increase biomass and bioactive compounds of G. procumbens adventitious roots. Therefore, a larger bioreactor for adventitious root culture is necessary to develop. In this study, the development of the 19 L pilot bioreactor was successful. The optimal condition for bioreactor sterilization is 1.8 bar for 60 min using an autoclave. We found that G. procumbens adventitious roots culture in pilot bioreactors has resulted in optimal biomass using MS-Tek media (technical grade) compared to MS-PA (pro analyze) media after 35 d of the culture period. Although, higher productivity of total phenolics and total flavonoids in G. procumbens roots has been achieved from MS-PA media rather than MS-Tek media. In further study, it is necessary to evaluate the effect of technical-grade chemicals on kinetics root growth and chemical uptake. The hope is to obtain a suitable media formulation with affordable cost for adventitious root culture production.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47741451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nephroprotective Effects of Cardamom Essential Oil (Amomum compactum Soland. Ex Maton) in Kidney Cells 豆蔻精油对肾细胞的保护作用

Indonesian Journal of Biotechnology Pub Date : 2023-06-27 DOI: 10.22146/ijbiotech.79642

Nisa Ul Hasanah, Ahmad Syauqy Tafrihani, Ummi Maryam Zulfin, D. Salsabila, Ratih Kurnia Wardani, Muthi’ Ikawati, E. Meiyanto, R. Jenie

{"title":"Nephroprotective Effects of Cardamom Essential Oil (Amomum compactum Soland. Ex Maton) in Kidney Cells","authors":"Nisa Ul Hasanah, Ahmad Syauqy Tafrihani, Ummi Maryam Zulfin, D. Salsabila, Ratih Kurnia Wardani, Muthi’ Ikawati, E. Meiyanto, R. Jenie","doi":"10.22146/ijbiotech.79642","DOIUrl":"https://doi.org/10.22146/ijbiotech.79642","url":null,"abstract":"Many chemotherapeutic agents cause various side effects, including nephrotoxicity. Doxorubicin, for example, increases the level of reactive oxygen species (ROS), leading to cell senescence in the kidneys. Cardamom essential oil (Amomum compactum Soland. ex Maton) contains compounds that exhibit antioxidant activity, such as 1.8-cineole, α-pinene, α-terpineol, and linalool. This study focused on exploring the potency of cardamom essential oil (CEO) as an anti-senescence induced by doxorubicin using Vero cells. CEO was obtained by steam distillation. The cytotoxic assay was carried out using trypan blue exclusion assay. We performed the 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) staining and the senescence-associated beta-galactosidase (SA-β-gal) staining to measure the effect of CEO on intracellular ROS level and cell senescence, respectively. Analysis of the compounds with gas chromatography-mass spectrophotometry (GC-MS), revealed seven compounds with significant abundance, namely 1.8-cineole (50.82%), ß-pinene (12.43%), α-terpineol (8.50%), fenchone (4.10%), α-pinene (4.00%), sabinene (3.00%), and linalool (1.98%). The cytotoxicity assay of CEO on Vero cells showed an IC50 value of 178 μg/mL. Thus, the CEO is considered to be not cytotoxic and safe for normal kidney cells. Concentrations of 50 and 100 μg/mL reduced the senescence induced by doxorubicin. Therefore, the CEO has the potency as a nephroprotective agent in doxorubicin-induced senescence. ","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48985604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of protease activity and anti‐quorum sensing of the potential fraction of ethanolic extract from Sansevieria trifasciata Prain leaves against Pseudomonas aeruginosa 三叶紫薇叶乙醇提取物对铜绿假单胞菌蛋白酶活性的抑制和潜在组分的抗群体感应

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.73649

W. Dewatisari, L. Nugroho, E. Retnaningrum, Y. A. Purwestri

{"title":"Inhibition of protease activity and anti‐quorum sensing of the potential fraction of ethanolic extract from Sansevieria trifasciata Prain leaves against Pseudomonas aeruginosa","authors":"W. Dewatisari, L. Nugroho, E. Retnaningrum, Y. A. Purwestri","doi":"10.22146/ijbiotech.73649","DOIUrl":"https://doi.org/10.22146/ijbiotech.73649","url":null,"abstract":"Sansevieria trifasciata is a plant that is commonly utilized in traditional medicine. The leaves of S. trifasciata show antibacterial properties against Pseudomonas aeruginosa. This bacterium is an opportunistic pathogen that can cause serious illness in humans and produce a variety of virulence factors responsible for bacterial pathogenesis with quorum sensing (QS) systems that mediate intracellular communication. Bacteria produce protease through a QS mechanism in which they express signaling molecules to become pathogens. Proteases are extracellular enzymes required for successful infection that mediate biofilm spread through QS and regulate a variety of cellular and physiological functions. This research aimed to evaluate the protease, and anti‐QS activities of the ethanolic extract from S. trifasciata leaves against P. aeruginosa and the expression of QS genes. An azocasein test was used to determine the protease activity in qualitative and quantitative methods. Using real‐time quantitative polymerase chain reaction, a study was conducted to investigate the effect of ethanolic extract from S. trifasciata leaves on selected QS‐regulatory genes at the transcriptional level. The results showed that the potential ethanolic extract from S. trifasciata leaves inhibited the protease enzyme activity by as much as 77.1%. The potential ethanolic extract from S. trifasciata leaves decreased the expressions of lasA, lasB, lasI, lasR, rhlI, and rhlR with 2‐ΔΔCt values of 0.81, 0.93, 0.76, 0.97, 0.90, and 0.55 respectively.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48950765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Production, purification and characterization of chitinase from Micromonospora sp. AR17 小单孢菌AR17几丁质酶的制备、纯化及特性研究

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.77137

{"title":"Production, purification and characterization of chitinase from Micromonospora sp. AR17","authors":"","doi":"10.22146/ijbiotech.77137","DOIUrl":"https://doi.org/10.22146/ijbiotech.77137","url":null,"abstract":"N‐acetylglucosamine (NAG) is the monomer product of chitin, which has been widely used as a bioactive com‐ pound in applications such as anti‐tumor, anti‐microbial, and antioxidant activities. In production, biological processes using enzymes are preferable to chemicals due to environmental issues. This study aims to determine the activity, purity level, and molecular weight of purified chitinase from Micromonospora sp. AR17 determines the concentration of NAG produced by purified chitinase that has been characterized. Chitinase was produced by fermentation in colloidal chitin broth at 40 °C, pH 7, for 7 days, while chitinase activity was checked every 24 h. The optimal fermentation time was used to produce chitinase for a further purification step. Enzyme purification was carried out by ultrafiltration, ammonium sulfate precipitation, ion exchange chromatography (Q Sepharose Fast Flow), and gel filtration (Sephacryl S‐300). The purified enzyme was then char‐ acterized for optimum time, pH, and temperature to produce NAG. The results suggested that the fourth day was the optimal time for chitinase production, with chitinase activity of 0.0040 U/mL and a NAG concentration of 7.62 µg/mL. The purifica‐ tion step successfully increased the purity by 6.82 times with chitinase‐specific activity at 1.4648 U/mg. Production of NAG with purified chitinase produced a NAG concentration of 32.472 µg/mL with an incubation time of 30 min at 40 °C and pH 7.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43292836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequence analyses of Indonesian isolates SARS‐CoV‐2 variants and their clinical manifestations 印尼SARS - CoV - 2分离株全基因组序列分析及其临床表现

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.73783

Elnora Listianto Lie, Tedi Dwi Fauzi Hermawan, Kholis Abdurachim Audah

{"title":"Whole genome sequence analyses of Indonesian isolates SARS‐CoV‐2 variants and their clinical manifestations","authors":"Elnora Listianto Lie, Tedi Dwi Fauzi Hermawan, Kholis Abdurachim Audah","doi":"10.22146/ijbiotech.73783","DOIUrl":"https://doi.org/10.22146/ijbiotech.73783","url":null,"abstract":"The SARS‐CoV‐2 virus has been the cause of the global pandemic since the end of 2019. Since then, the virus has mutated to create different types of variants with numerous effects on those infected. This has complicated human intervention for prevention. Indonesia was heavily affected by the pandemic, specifically from May to August 2021, and as a country has recorded many distinct isolates. Thus, characterization of the virus strains from Indonesia is important. GISAID, NCBI BLAST, and MAFFT version 7 were used. There were 9,488 isolates in Indonesia as of November 2021, with the majority including the Delta variant. While most of the isolates have mutations common to those from other countries, there are some atypical ones, such as mutation V1264L in the Delta variant that was suspected to play a role in worsening the pandemic. The Delta variant had the most mutations in the spike protein when compared to the Alpha and Beta variants, giving it important roles in infectivity and vigorous entry into cells, with some general clinical manifestations like fever and sore throat; however, the severity of the Delta variant is attributable to its rapid growth. This is why, from May to November 2021 in Indonesia, cases of the Delta variant rocketed, unlike the other variants.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135733142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease 一种鸡抗体在甘蔗花叶病毒病免疫检测中的应用效果

Indonesian Journal of Biotechnology Pub Date : 2023-03-31 DOI: 10.22146/ijbiotech.74104

Nurmalasari Darsono, W. D. Sawitri, Retnosari Apriasti, Agus Wahyudi, Putri Andreyna Saragi, Victorin Mega Putri, S. Sugiharto, W. Darmanto

{"title":"The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease","authors":"Nurmalasari Darsono, W. D. Sawitri, Retnosari Apriasti, Agus Wahyudi, Putri Andreyna Saragi, Victorin Mega Putri, S. Sugiharto, W. Darmanto","doi":"10.22146/ijbiotech.74104","DOIUrl":"https://doi.org/10.22146/ijbiotech.74104","url":null,"abstract":"Sugarcane Mosaic Virus (SCMV) infection is one of the most serious problems that can result in severe yield loss of sugarcane. Since the symptoms of SCMV infection are similar to other biotic and abiotic stress symptoms, the development of a rapid diagnostic with high precision is required. The use of laboratory animals such as rabbits is required for antibody production in immunoassay‐based detection. However, due to its many advantages, specific chicken egg yolk immunoglobulin (IgY) has received considerable attention as an alternative antibody production in immunodiagnostics for infectious diseases. In this study, IgY antibody against SCMV recombinant coat protein (CP) was successfully obtained from chicken blood serum and tested to compare its efficacy against antibody from rabbit (IgG) using immunocapture reverse transcription‐polymerase chain reaction (IC‐RT‐PCR). The result showed that IgY and IgG could detect 0.1 g SCMV infected leaves using 1000‐times‐diluted antibodies. The IgY antibody was also confirmed to be reproducible and potentially applicable in plant disease diagnostics using an antibody‐based detection.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44118816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxic mesenchymal stem cell‐conditioned medium accelerates wound healing by regulating IL‐10 and TGF‐β levels in a full‐thickness‐wound rat model 在全厚度创伤大鼠模型中，缺氧间充质干细胞条件培养基通过调节IL-10和TGF-β水平加速创伤愈合

Indonesian Journal of Biotechnology Pub Date : 2022-12-30 DOI: 10.22146/ijbiotech.63914

Adi Muradi Muhar, Faizal Mukharim, Dedy Hermansyah, A. Putra, Nurul Hidayah, N. Amalina, I. Alif

{"title":"Hypoxic mesenchymal stem cell‐conditioned medium accelerates wound healing by regulating IL‐10 and TGF‐β levels in a full‐thickness‐wound rat model","authors":"Adi Muradi Muhar, Faizal Mukharim, Dedy Hermansyah, A. Putra, Nurul Hidayah, N. Amalina, I. Alif","doi":"10.22146/ijbiotech.63914","DOIUrl":"https://doi.org/10.22146/ijbiotech.63914","url":null,"abstract":"Full‐thickness wound healing is a complex process requiring a well‐orchestrated mechanism of various factors, including cytokines, particularly interleukin (IL)‐10 and transforming growth factor (TGF)‐β. IL‐10 and TGF‐β act as robust anti‐inflammatory cytokines in accelerating the wound healing process by regulating myofibroblasts. Hypoxic mesenchymal stem cell‐conditioned medium (hypMSC‐CM) containing cytokines potentially contribute to accelerate wound repair without scarring through the paracrine mechanism. This study aims to observe the role of hypMSC‐CM in controlling TGF‐β and IL‐10 levels to accelerate full‐thickness wound repair and regeneration. A total of 24 male Wistar rats were used in this study. Six healthy rats as a sham group and 18 rats were created as full‐thickness‐wound animal models using a 6 mm punch biopsy. The animals were randomly assigned into three groups (n = 6) consisting of two treatment groups treated with hypMSC‐CM at a low dose (200 µL hypMSC‐CM with 2 g water‐based gel added) and a high dose (400 µL hypMSC‐CM with 2 g water‐based gel added) and a control group (2 g water‐based gel only). The IL‐10 and TGF‐β levels were examined by ELISA. The results showed a significant increase in IL‐10 levels on day 3 after hypMSC‐CM treatment, followed by a decrease in platelet‐derived growth factor (PDGF) levels on days 6 and 9. In line with this finding, the TGF‐β levels also increased significantly on day 3 and then linearly decreased on days 6 and 9. HypMSC‐CM administra‐ tion may thus promote wound healing acceleration by controlling IL‐10 and TGF‐β levels in a full‐thickness‐wound rat model.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44183714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carrot hairy roots (Daucus carota L.) characterisation and optimisation for high β‐carotene extraction 胡萝卜毛状根(Daucus carota L.)的表征及高β -胡萝卜素提取的优化

Indonesian Journal of Biotechnology Pub Date : 2022-12-30 DOI: 10.22146/ijbiotech.73476

N. T. Mai, Thi Van Anh Le, B. C. Nguyen, Nguyen Ha Trang Le, Quang Minh Do

{"title":"Carrot hairy roots (Daucus carota L.) characterisation and optimisation for high β‐carotene extraction","authors":"N. T. Mai, Thi Van Anh Le, B. C. Nguyen, Nguyen Ha Trang Le, Quang Minh Do","doi":"10.22146/ijbiotech.73476","DOIUrl":"https://doi.org/10.22146/ijbiotech.73476","url":null,"abstract":"Hairy roots are widely known as a biological system for the production of highly diverse biomolecules. β‐carotene – a precursor for vitamin A – is known to be an anti‐oxidant and anti‐gastric cancer and protection agent against cardiovascular disease, heart disease and stroke. β‐carotene has been chemically synthesised and consumed by humans. However, the chemical process often produces a by‐product that may be harmful to human health. Therefore, this study established a protocol to induce hairy roots (HRs) from a Vietnamese carrot variety and produce natural β‐carotene. The Rhizobium rhizogenes ATCC15834 harbouring Ri plasmid and a Vietnamese carrot variety were used as materials for genetic transformation and HR induction studies. The result showed that approximately 50 HR lines were obtained. Culture medium supplemented with 30 mg/L of sucrose that gave the highest biomass of HR was shown in carrot HR line 30, which had a doubling time of 6.5 days. The highest content of β‐carotene extraction, at 128 mg/100g hairy roots, was achieved with a ratio volume (v/v) of 2‐propanol and plant samples of 20:1, followed by two hours’ incubation with 2‐propanol at 60 °C. Our study reveals a highly efficient protocol for Vietnamese carrot hairy root establishment and multiplication. A very efficient protocol for β‐carotene extraction from the hairy root was established to produce natural β‐carotene that achieves the same β‐carotene quantity as that produced by normal roots. This study provides new insight into the production of high‐content and natural β‐carotene for therapeutic application.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45089616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Anti‐diabetic effect of andrographolide from Sambiloto herbs (Andrographis paniculata (Burm.f.) Nees) through the expression of PPARγ and GLUT‐4 in adipocytes 桑比洛草药穿心莲内酯通过脂肪细胞中PPARγ和GLUT‐4表达的抗糖尿病作用

Indonesian Journal of Biotechnology Pub Date : 2022-12-30 DOI: 10.22146/ijbiotech.68800

Novi Tri Astuti, P. Novitasari, R. Tjandrawinata, A. Nugroho, S. Pramono

{"title":"Anti‐diabetic effect of andrographolide from Sambiloto herbs (Andrographis paniculata (Burm.f.) Nees) through the expression of PPARγ and GLUT‐4 in adipocytes","authors":"Novi Tri Astuti, P. Novitasari, R. Tjandrawinata, A. Nugroho, S. Pramono","doi":"10.22146/ijbiotech.68800","DOIUrl":"https://doi.org/10.22146/ijbiotech.68800","url":null,"abstract":"Andrographolide has been shown to have a pharmacological effect as an antidiabetic. Nevertheless, the comprehensive mechanism of action has yet to be determined. Andrographolide is a primary component of the sambiloto herb (Andrographis paniculata (Burm.f.) Nees), in which a simple isolation process can obtain high yields. This study aimed to explain the anti‐diabetic effect of andrographolide compared to pioglitazone (a positive control) on glucose uptake by measuring the expression levels of peroxisome proliferator‐activated receptor gamma (PPARγ) and glucose transporter type 4 (GLUT‐4) genes in 3T3‐LI mouse adipocytes as an in vitro model. The differentiation of mature adipocytes from 3T3‐L1 fibroblasts was induced with 3‐isobutyl‐1‐methylxanthine, dexamethasone, and insulin. Andrographolide was provided through direct isolation from A. paniculata herbs. The gene expression was detected using the reverse transcription‐polymerase chain reaction (RT‐PCR). Pioglitazone and andrographolide significantly increased glucose uptake capability. Andrographolide was able to increase the mRNA levels of PPARγ and GLUT‐4 compared to pioglitazone with the best concentration at 5.6 µM. In conclusion, andrographolide can improve glucose uptake by increasing mRNA levels of PPARγ and GLUT‐4 that encodes protein, which are key factors for glucose homeostasis. Therefore, this finding further establishes the potency of andrographolide from A. paniculata as an antidiabetic.","PeriodicalId":13452,"journal":{"name":"Indonesian Journal of Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44546373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of ultrasonic processing on physical and chemical properties of milk‐based soft, brine cheese 超声波加工对乳基软质盐水奶酪理化性质的影响

Indonesian Journal of Biotechnology Pub Date : 2022-12-30 DOI: 10.22146/ijbiotech.73930

A. Kadi, U. Bagale, I. Potoroko

引用次数: 1