Natural and Life Sciences Communications最新文献_第3页

Cleistocalyx nervosum var. paniala Extract: Anti-acne, Anti-oxidant, Anti-inflammatory Activities and Safety for Cosmeceutical Applications 闭锁花提取物:抗痤疮、抗氧化、抗炎活性和药妆安全性

Natural and Life Sciences Communications Pub Date : 2023-08-30 DOI: 10.12982/nlsc.2023.064

Tharadon Khammitham, P. Leelapornpisid, S. Chansakaow, Warat Leelapornpisid, Worrapan Poomanee

{"title":"Cleistocalyx nervosum var. paniala Extract: Anti-acne, Anti-oxidant, Anti-inflammatory Activities and Safety for Cosmeceutical Applications","authors":"Tharadon Khammitham, P. Leelapornpisid, S. Chansakaow, Warat Leelapornpisid, Worrapan Poomanee","doi":"10.12982/nlsc.2023.064","DOIUrl":"https://doi.org/10.12982/nlsc.2023.064","url":null,"abstract":"This research aimed to investigate the biological activities and safety of extracts from Cleistocalyx nervosum var. paniala for anti-acne cosmeceutical and to determine total phenolic, flavonoid and anthocyanin contents and HPLC fingerprint. The extracts named as whole fruit extract (WF) and seed extract (SE), were macerated with 95% ethanol. Antibacterial effects against Cutibacterium acnes and Staphylococcus aureus were evaluated using agar diffusion, broth microdilution and time-kill kinetic assays. The results revealed that SE presented stronger antibacterial activities against C. acnes and S. aureus with inhibition zone of 22.74 ± 0.52 and 12.67 ± 0.15 mm, respectively. However, the MIC and MBC values were with 6.25 and 3.12 mg/ml for C. acnes and S. aureus, respectively, with a kill time of 2 and 4 h, respectively. SE showed higher phenolic and flavonoid contents compared with WF. In contrast, WF revealed higher anthocyanin content. According to HPLC analysis, ellagic acid was used as a marker. Additionally, anti-oxidant activity of the extracts was investigated by DPPH assay and inhibition on nitric oxide radicals. SE showed better activities with IC50 of 7.51 ± 0.40 and 25.47 ± 1.09 µg/ml, respectively. Therefore, SE was selected for testing with nitric oxide radical producing on RAW 264.7 cell lines which presented 22.99% inhibition. Moreover, SE showed no irritation on Hen's Egg Test Chorioallantoic Membrane. To conclude, SE from C. nervosum exerted stronger biological activities than WF. It also demonstrated a good potential concerning antibacterial on acne-related microorganisms, anti-oxidants, and anti-inflammatory activities. Therefore, it could be a promising bio-active compound to further develop into anti-acne cosmeceutical products. Keywords: Cleistocalyx nervosum, Acne vulgaris, Cutibacterium acnes, Anti-inflammatory activity","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115423532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of Novel Floating 3D-Printed Devices with Adjustable Releasing Channels for Controlled Release 具有可调节释放通道的新型浮动3d打印装置的制造

Natural and Life Sciences Communications Pub Date : 2023-08-25 DOI: 10.12982/nlsc.2023.063

Thapakorn Charoenying, P. Akkaramongkolporn, P. Opanasopit, Prasopchai Patrojanasophon, S. Pengnam

{"title":"Fabrication of Novel Floating 3D-Printed Devices with Adjustable Releasing Channels for Controlled Release","authors":"Thapakorn Charoenying, P. Akkaramongkolporn, P. Opanasopit, Prasopchai Patrojanasophon, S. Pengnam","doi":"10.12982/nlsc.2023.063","DOIUrl":"https://doi.org/10.12982/nlsc.2023.063","url":null,"abstract":"Gastro-retentive drug delivery systems (GRDDS) have been used to improve the therapeutic efficacy of drugs with narrow absorption windows, unstable in alkaline pH, soluble in acidic conditions, and active locally in the stomach. This study aimed to produce a novel floating three-dimensional (3D)-printed device (F3D) from polylactic acid filaments using a fused deposition modeling 3D printer. There were two parts of F3D: a cap with an air chamber for floating property and a body with 6 channels for controlled release. The device was designed to have adjustable channels for the releasing control and gastric retention of Bromhexine, carvedilol, and theophylline. The releasing channels could be adjusted by rotating the base to open or close the number of releasing channels as 3 levels (2, 4, and 6 channels). Morphology, weight variation, ex vivo floating time, and drug release characteristics were examined. The F3D had a smooth texture with a narrow SD value of weight variation and actual size, suggesting that the F3D had high accuracy and consistent fabrication using 3D printing technology. All tablets incorporated F3D was able to float for at least 24 h. Decreasing the number of channels on the devices led to the sustained release of drugs following 2> 4> 6 channels. Hence, F3D could be useful in controlling drug release for various commercial tablets. Keywords: 3D-printed device, Floating device, Fused deposition modeling, Gastroretentive drug delivery systems, controlled release dosage form","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132850433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Antidesma thwaitesianum Müll. Arg. Fruit Extract on Radical Scavenging and Apoptotic Properties 抗霉变剂的作用研究[j]。参数。水果提取物对自由基清除和细胞凋亡的影响

Natural and Life Sciences Communications Pub Date : 2023-08-23 DOI: 10.12982/nlsc.2023.062

Supaporn Khamchun, Natthapak Sillawatthumrong, W. Insuan, W. Suttana, Daoyot Daorueang, Plubplung Sansai, Orapin Insuan

{"title":"Effects of Antidesma thwaitesianum Müll. Arg. Fruit Extract on Radical Scavenging and Apoptotic Properties","authors":"Supaporn Khamchun, Natthapak Sillawatthumrong, W. Insuan, W. Suttana, Daoyot Daorueang, Plubplung Sansai, Orapin Insuan","doi":"10.12982/nlsc.2023.062","DOIUrl":"https://doi.org/10.12982/nlsc.2023.062","url":null,"abstract":"Antidesma thwaitesianum Müll. Arg. is one of a tropical fruit which is a good source of phenolic compounds. Here, we investigated the in vitro radical scavenging activity and effect of chlorogenic acid (CGA) and A. thwaitesianum fruit extract (AFE) against H2O2-induced cytotoxicity in RAW264.7 macrophage cells. Chemical components in the extract were analyzed by colorimetric method and high performance liquid chromatography. The results exhibited that the amount of total phenolic and total flavonoid contents in AFE were 25.486 ± 0.370 mg of gallic acid equivalents (GAE)/g extract and 7.264 ± 0.312 mg of catechin equivalents (CE)/g extract, respectively, while 114 μg/g crude extract CGA was detected in AFE. AFE showed scavenging activity against ABTS and DPPH radicals, with a 50% inhibition concentration of 11.435 ± 0.381 and 2.063 ± 0.022 mg/mL, respectively. Pretreatment with both CGA (10 µg/mL) and AFE (400 µg/mL) significantly restored cell viability in H2O2-induced cytotoxicity in RAW264.7 macrophage cells, and only CGA at the concentrations of 5 and 10 µg/mL could protect cells from apoptosis. In conclusion, CGA-containing AFE has potent in vitro antioxidant activity and can increase the cell survival in H2O2-induced RAW264.7 macrophage cells. These results support the nutritional and medical value of A. thwaitesianum fruit for health-promoting benefits. Keywords: Antidesma thwaitesianum Müll. Arg., Chlorogenic acid, Antioxidant activity, Anti-apoptotic activity, RAW264.7 macrophage cells","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127858969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nigella sativa Extract on Gingival Epithelium Exposed to LPS Porphyromonas gingivalis and Its Impact on the Expression of TLR-4 and NF-kB in vivo Study 黑草提取物对LPS牙龈卟啉单胞菌牙龈上皮的影响及其对TLR-4和NF-kB表达的体内研究

Natural and Life Sciences Communications Pub Date : 2023-08-21 DOI: 10.12982/nlsc.2023.061

S. K. Supandi, Andry Elvandari, Lambang Bargowo, I. Wijaksana

{"title":"Nigella sativa Extract on Gingival Epithelium Exposed to LPS Porphyromonas gingivalis and Its Impact on the Expression of TLR-4 and NF-kB in vivo Study","authors":"S. K. Supandi, Andry Elvandari, Lambang Bargowo, I. Wijaksana","doi":"10.12982/nlsc.2023.061","DOIUrl":"https://doi.org/10.12982/nlsc.2023.061","url":null,"abstract":"Porphyromonas gingivalis (P. gingivalis) is capable of enhancing oral dysbiosis resulting in an imbalance between beneficial commensal and periodontal pathogenic bacteria that causes chronic inflammation. Nigella sativa (N. sativa) is a type of herbal plant with antioxidant, anti-inflammatory, antimicrobial, anticancer, and other properties. One of the main active ingredients is thymoquinone. Researchers conducted this research to determine the impact of using Nigella sativa as a component in the prevention or treatment of periodontal disease. This research included a lab experiment using a randomized post-test-only control group design. The animals used were male white Wistar rats (Rattus norvegicus), aged 10-11 weeks, and weighing approximately 150-170 grams. All of them received adaptation for a week. Afterward, 45 of them were randomly allocated into three research groups: control group C (LPS P. gingivalis), group E1 (N. sativa extract + LPS), and group E2 (LPS + N. sativa). The rats were terminated after 7, 14, and 21 days and immunohistochemistry was performed using monoclonal antibody anti-TLR-4 and monoclonal antibody anti-NF-kB. The results showed that TLR-4 and NF-κB increased in group C and decreased in E1 and E2 groups. Although it was not statistically significant, the TLR-4 and NF-kB expression decreased more in the E2 group than in the E1 group. The result of this research shows that Nigella sativa can reduce the expression of TLR-4 and NF-kB as a part of the host’s innate defense. Keywords: Nigella sativa, LPS Porphyromonas gingivalis, TLR-4, NF-kB, Periodontal disease","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115160290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant, Antibacterial Activities and Cytotoxicity of Garlic Leaf Extract from Garlic Waste 大蒜叶提取物的抗氧化、抗菌活性及细胞毒性研究

Natural and Life Sciences Communications Pub Date : 2023-08-08 DOI: 10.12982/nlsc.2023.059

P. Sirisa-ard, Kiatisak Pholsonklam, Apinya Satchachai, Y. Tragoolpua, Thida Kaewkod

{"title":"Antioxidant, Antibacterial Activities and Cytotoxicity of Garlic Leaf Extract from Garlic Waste","authors":"P. Sirisa-ard, Kiatisak Pholsonklam, Apinya Satchachai, Y. Tragoolpua, Thida Kaewkod","doi":"10.12982/nlsc.2023.059","DOIUrl":"https://doi.org/10.12982/nlsc.2023.059","url":null,"abstract":"Five garlic parts (root, clove skin, leaf, flower stalk and garlic peel) of garlic waste from garlic harvest processing were used in this study. Each part of the garlic waste was screened for total phenolic, flavonoid and antioxidant activity after extraction with 70% ethanol. Interestingly, garlic leaf (GLf) showed the greatest richness of total phenolic, flavonoid and antioxidant activity. GLf was selected to extract with water and 70% ethanol and the highest extraction yield was achieved with water extract. The GLf extracts demonstrated a good quality standard of physical and chemical properties. The aqueous extract of GLf revealed the highest total phenolic content of 1,676.40 ± 18.02 mg GAE/100 g extract. Both aqueous and ethanolic extracts did not differ regarding total flavonoid contents, showing values of 118.60 ± 6.10 mg QUE/100 g extract and 110.90 ± 2.30 mg QUE/100 g extract, respectively. Phenolic compounds including gallic acid, catechin and tannic acid were detected in the aqueous and ethanolic extracts of GLf by HPLC assay. Moreover, four flavonoid compounds including isoquercetin, quercetin, kaempferol and rutin were detected in aqueous extract whereas the ethanolic extract presented apigenin, isoquercetin, quercetin and kaempferol. The ethanolic extract of GLf demonstrated the stronger antioxidant activity than aqueous extract. GLf extracts also exhibited antimicrobial activity against pathogenic bacteria with MIC and MBC values of 125-250 mg/mL. In addition, GLf aqueous extract showed lower toxicity on Vero cells than ethanolic extract. Therefore, GLf waste can be used as a cheap source of natural compounds and antioxidants, with potential applications in different biological, medicinal and food products. Keywords: Allium sativum L., Antibacteria, Antioxidant, Garlic, Toxicity","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123214576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age Estimation Using the Demirjian and Willems Methods in a Southern Thai Population 使用Demirjian和Willems方法估算泰国南部人群的年龄

Natural and Life Sciences Communications Pub Date : 2023-08-08 DOI: 10.12982/nlsc.2023.058

Pornpat Theerasopon, Chairat Charoemratrote, Phuwadon Duangto

{"title":"Age Estimation Using the Demirjian and Willems Methods in a Southern Thai Population","authors":"Pornpat Theerasopon, Chairat Charoemratrote, Phuwadon Duangto","doi":"10.12982/nlsc.2023.058","DOIUrl":"https://doi.org/10.12982/nlsc.2023.058","url":null,"abstract":"The objective of this study was to test the accuracies of the Demirjian and the Willems dental age estimation methods using the seven left permanent mandibular teeth (excluding the third molar tooth) in children in southern Thailand. Digital panoramic radiographs of 240 Thai children (120 males and 120 females) aged from 8 to 15 years were chosen and separated into eight age groups. Dental age was assessed using the Demirjian and the Willems methods. Differences between the dental age and the chronological age were statistically evaluated by the Wilcoxon signed-rank test. The results showed that the Demirjian method overestimated age by 0.28 years and 0.24 years for males and females, respectively, while the Willems method underestimated age by −0.04 years and −0.23 years for males and females, respectively. Moreover, the mean absolute errors were 0.68 years in males and 0.56 years in females for the Demirjian method and 0.55 years in males and 0.60 years in females for the Willems method. In conclusion, the Willems method was more accurate for dental age estimation compared to the Demirjian method. However, both methods can be applied to estimate the age of children in southern Thailand since the mean error and mean absolute error values were less than one year. Keywords: Dental age estimation, Demirjian method, Tooth developmental stage, Thai population, Willems method","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127310760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity, Apoptosis Induction, Oxidative Stress, and Cell Cycle Arrest of Clerodendrum chinense Flower Extract Nanoparticles in HeLa Cells 棘花提取物纳米颗粒在HeLa细胞中的细胞毒性、凋亡诱导、氧化应激和细胞周期阻滞

Natural and Life Sciences Communications Pub Date : 2023-08-08 DOI: 10.12982/nlsc.2023.057

Chuda Chittasupho, W. Samee, S. Tadtong, Wullapa Jittachai, C. Managit, Sirivan Athikomkulchai

{"title":"Cytotoxicity, Apoptosis Induction, Oxidative Stress, and Cell Cycle Arrest of Clerodendrum chinense Flower Extract Nanoparticles in HeLa Cells","authors":"Chuda Chittasupho, W. Samee, S. Tadtong, Wullapa Jittachai, C. Managit, Sirivan Athikomkulchai","doi":"10.12982/nlsc.2023.057","DOIUrl":"https://doi.org/10.12982/nlsc.2023.057","url":null,"abstract":"Clerodendrum chinense is a plant containing numerous secondary active metabolites. This study aimed to evaluate the antioxidant and anti-cancer activities of C. chinense flower ethanolic extract and identify the bioactive compounds. High-performance liquid chromatography was used to identify and quantify the bioactive compounds of the extract. The antioxidant activity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assays and the Ferric Reducing Antioxidant Power Assay (FRAP). The cytotoxicity of C. chinense flower ethanolic extract against A549, MCF-7 and HeLa cells was determined using the sulforhodamine B (SRB) assay. Nanoparticles (NPs) of C. chinense flower extract were fabricated using a solvent displacement method. Then the anti-cancer activities of the extract and NPs were confirmed by cytotoxicity, cell apoptotic induction, reactive oxygen species (ROS) formation, colony formation and cell cycle assays against HeLa cervical cancer cells. C. chinense flower extract and NPs showed significant free radical scavenging activities and ferric-reducing power. As shown by Pearson’s correlation coefficients, the total phenolic and total flavonoid contents of C. chinense flower extract and NPs significantly correlated with antioxidant activity. C. chinense flower extract demonstrated antiproliferative activity against A549, MCF-7 and HeLa cancer cells after treatment for 24, 48 and 72 h. The growth inhibition of HeLa cells mediated by C. chinense flower extract and NPs appears to be associated with apoptosis, ROS formation and cell cycle arrest. Thus, this study provided evidence of the anticervical cancer potentials of C. chinense flower ethanolic extract and NPs. Keywords: Natural products, Nanoparticles, Pharmaceutical, Phenolic compounds, Plant extract, Clerodendrum chinense","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133165441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Electrolyzed Reducing Water on Pesticides Reduction and Postharvest Qualities in Cherry Tomato 电解还原水对樱桃番茄减毒及采后品质的影响

Natural and Life Sciences Communications Pub Date : 2023-08-08 DOI: 10.12982/nlsc.2023.056

W. Pattanapo, Kanda Wanchai, U. Chanasut

{"title":"Effects of Electrolyzed Reducing Water on Pesticides Reduction and Postharvest Qualities in Cherry Tomato","authors":"W. Pattanapo, Kanda Wanchai, U. Chanasut","doi":"10.12982/nlsc.2023.056","DOIUrl":"https://doi.org/10.12982/nlsc.2023.056","url":null,"abstract":"This study investigated the effect of electrolyzed reducing water (ERW) on the reduction of profenofos (PF) and carbendazim (CBZ) residue of cherry tomato (Solanum lycopersicum var. cerasiforme). Organic tomato were soaked with pesticides solution (PF and CBZ) at 5 mg L-1, then air dried at room temperature. Thereafter, the treated fruits were washed with ERW at different concentrations (50, 100, 250, and 500 mg L-1) for various times (10, 20, and 30 min). The result found that the removal percentages of standard profenofos and carbendazim were significantly induced with ERW 500 mg L-1 for 20 min. Wherewith, the profenofos released the substance to pyrocatechol and 4-Methylphenol (p-Cresol), and carbendazim was degraded to a N-Acetyl-2,6-diethylaniline. The removal percentage of profenofos and carbendazim residues in tomato was found to be possible to effectively reduce residues 71.54% and 80.49%, respectively. On the other hand, distilled water (DW) could achieve removal of only 21.67%. The quality of the tomato stored at 25ºC for 8 days showed that not significant changes in weight loss, firmness, total soluble solids (TSS), titratable acidity (TA), lycopene content, and visual appearance for both pesticides. Keywords: Electrolyzed reducing water, Profenofos, Carbendazim, Cherry tomato","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"13 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116865769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved PCR for Detection of Xanthomonas euvesicatoria pv. perforans in tomato seeds 灰枯黄单胞菌PCR检测方法的改进。番茄种子中的穿孔

Natural and Life Sciences Communications Pub Date : 2023-07-10 DOI: 10.12982/nlsc.2023.054

J. Watcharachaiyakup, Kanchanaphon Sawangchaitham, P. Burns, W. Kositratana

{"title":"Improved PCR for Detection of Xanthomonas euvesicatoria pv. perforans in tomato seeds","authors":"J. Watcharachaiyakup, Kanchanaphon Sawangchaitham, P. Burns, W. Kositratana","doi":"10.12982/nlsc.2023.054","DOIUrl":"https://doi.org/10.12982/nlsc.2023.054","url":null,"abstract":"Abstract The detection efficiency of the PCR methods for Xanthomonas euvesicatoria pv. perforans (Xep) was determined. Xep specific primer set; HpaF-f/HpaF-r was compared with the previously reported Bs-XpF/Bs-XpR primers in multiplex PCR reaction and singleplex PCR for detection of Xep were tested. The improvement of PCR amplification efficacy was investigated by adding PCR additives or either 5% DMSO or 1 M betaine. Adding both PCR additives gave the same lowest detection limit of Xep detection in both multiplex and singleplex PCR at 100 fg. However, multiplex PCR gave no equal amplification rate for both sets of primers and also showed cross-amplification to X. vesicatoria. Then singleplex PCR with both PCR additives was further tested in a tomato seed lot. The artificially inoculated tomato seeds with various concentrations of Xep from 1.05 x 105 to 101 CFU/ml were tested with singleplex PCR. The lowest detection limit was 101 CFU/ml of the 5 replications of each inoculated seed. One artificially inoculated tomato seed from each bacterial cell suspension was mixed with 2,000 healthy seeds making 0.05% (1/2,000) contaminated tomato seeds sample and further tested with singleplex PCR, results showed the detection limit of 101 CFU/ml in 0.05% Xep contaminated seed lot. Keywords: PCR additives, Seed, Bacterial leaf spot, Tomato, Conventional PCR","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133714069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Longan Biochar as Filter Materials on Plant Responses and Wastewater Treatment by Typha angustifolia L. 龙眼生物炭作为滤料对植物反应及废水处理的影响

Natural and Life Sciences Communications Pub Date : 2023-07-10 DOI: 10.12982/nlsc.2023.035

Pakawat Janyasupab, H. Brix, A. Jampeetong

{"title":"Effects of Longan Biochar as Filter Materials on Plant Responses and Wastewater Treatment by Typha angustifolia L.","authors":"Pakawat Janyasupab, H. Brix, A. Jampeetong","doi":"10.12982/nlsc.2023.035","DOIUrl":"https://doi.org/10.12982/nlsc.2023.035","url":null,"abstract":"Abstract This study investigated the effects of filter materials and Typha angustifolia L. (cattail) plantation on wastewater treatment efficiency and assessed the growth responses of cattail in the different filter materials. Five treatments of lab-scale experiment were set-up: unplanted and planted columns filled with either gravel or longan biochar, and columns without any filters and plants were used as a control. Without plantation, longan biochar filters removed 97% of biochemical oxygen demand (BOD5), 96% of total suspended solids (TSS), 86% of ammonium (NH4-N), 82% of total Kjeldahl nitrogen (TKN), and 75% of nitrate (NO3-N). Compared with the unplanted gravel-based system, 87% of BOD5 and 89% of TSS were removed but NH4-N, TKN, and NO3-N removal was only 27%, 37%, and 34%, respectively. However, the pollutant removal in gravel-based systems was as high as the removal in longan biochar-based systems when planted with cattail. It removed 84% and 81% of NH4-N and TKN, respectively, and removed 90% of total phosphorus (TP) whereas the TP removals in the other four treatments were only 13-25%. The study indicates that longan biochar is efficient for removing N and for filtering organic matters from wastewater. Hence, longan biochar may have promise for the use as a filter material in constructed wetlands (CWs). The biochar, however, may have adverse effects on the plants. Therefore, the proportion of biochar in the filter material must be optimized to obtain the most effective plant growth and water treatment before the system can be applied in real-life systems. Keywords: Cattail, Longan biochar, Gravel, Nutrient, Wastewater treatment","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126156188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0