{"title":"Refinement analysis with clustering segmentation for port wine stains","authors":"Lin-huan He, Yi Zhao, Jining Tao, Xiaoming Hu","doi":"10.1117/12.2507010","DOIUrl":"https://doi.org/10.1117/12.2507010","url":null,"abstract":"Although both flashlamp-pumped pulsed dye laser and vascular-targeted photodynamic therapy have demonstrated safety and efficacy for port wine stains (PWS), the clinical results of these methods may be limited because the laser parameters used are virtually identical for all PWS patients despite their variations from site to site. A method that combines digital color-image processing with clustering segmentation based on color similarity and spatial proximity is proposed to refine the severity grading of PWS lesions. The evaluation in the color spaces of CIE-L*a*b*, value of a*, and erythema index are analyzed and compared, and the severity level grading is refined with the reference of a 10-level red color bar. The proposed clustering method can be used as a detailed objective assessment for PWS with a low-cost camera.","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121856071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Front Matter: Volume 10851","authors":"","doi":"10.1117/12.2531179","DOIUrl":"https://doi.org/10.1117/12.2531179","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130689772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hanna Jonasson, Ingemar Fredriksson, Sara Bergstrand, C. Östgren, R. Saager, M. Larsson, Tomas Stroemberg
{"title":"In vivo absorption and scattering properties of human skin: a cohort-based study (Conference Presentation)","authors":"Hanna Jonasson, Ingemar Fredriksson, Sara Bergstrand, C. Östgren, R. Saager, M. Larsson, Tomas Stroemberg","doi":"10.1117/12.2508814","DOIUrl":"https://doi.org/10.1117/12.2508814","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116955921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Machine learning approach to synthesizing multiphoton microscopic images from reflectance confocal (Conference Presentation)","authors":"A. C. Mugdha, Jesse W. Wilson","doi":"10.1117/12.2510473","DOIUrl":"https://doi.org/10.1117/12.2510473","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122173093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ponticorvo, Rebecca Rowland, D. Burmeister, R. Christy, N. Bernal, Anthony J. Durkin
{"title":"Direct comparison of spatial frequency domain imaging, laser speckle imaging and thermal imaging to standard of care clinical assessment in a graded model of burn severity (Conference Presentation)","authors":"A. Ponticorvo, Rebecca Rowland, D. Burmeister, R. Christy, N. Bernal, Anthony J. Durkin","doi":"10.1117/12.2510609","DOIUrl":"https://doi.org/10.1117/12.2510609","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126896422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Wang, Wen-zhi Cai, Kezhi Kong, Xueyuan Jia, R. R. Anderson, W. Franco
{"title":"Visualization of neo-epidermis formation and evaluation of wound closure using UV fluorescence excitation imaging at two wavelengths (Conference Presentation)","authors":"Ying Wang, Wen-zhi Cai, Kezhi Kong, Xueyuan Jia, R. R. Anderson, W. Franco","doi":"10.1117/12.2511329","DOIUrl":"https://doi.org/10.1117/12.2511329","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129715298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giselle Tian, H. Lui, Jianhua Zhao, Zhenguo Wu, S. Kalia, V. Richer, I. Seo, Hao Ouyang, H. Zeng
{"title":"Tracking cellular dynamics of human skin responses to UV exposure using in vivo multimodal microscopy (Conference Presentation)","authors":"Giselle Tian, H. Lui, Jianhua Zhao, Zhenguo Wu, S. Kalia, V. Richer, I. Seo, Hao Ouyang, H. Zeng","doi":"10.1117/12.2513734","DOIUrl":"https://doi.org/10.1117/12.2513734","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115427389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Lentsch, M. Balu, K. Koenig, S. Jo, B. Tromberg, A. Ganesan
{"title":"In vivo multiphoton microscopy imaging of vitiligo (Conference Presentation)","authors":"G. Lentsch, M. Balu, K. Koenig, S. Jo, B. Tromberg, A. Ganesan","doi":"10.1117/12.2508791","DOIUrl":"https://doi.org/10.1117/12.2508791","url":null,"abstract":"Vitiligo is a skin condition in which pigment-producing cells are removed by the immune system, leading to patches of white skin on different parts of the body. Treatments, including UVB light therapy and skin micro-grafting, may lead to repigmentation of the skin; however, treatments are not uniformly successful, and it is currently unclear why some vitiligo areas repigment more rapidly than others. An optical imaging technique that allows non-invasive visualization of melanocytic activity in skin may advance the knowledge about this skin condition and help understand treatment impact. In this pilot study, we employ in-vivo multiphoton microscopy (MPM) to evaluate architectural and structural features of the melanocytes that repigment vitiligo skin. MPM is a nonlinear laser scanning microscopy technique that features sub-cellular resolution and label-free molecular contrast. MPM contrast in skin is derived from two-photon excited fluorescence of NADH/FAD+, keratin, melanin, and elastin, and second-harmonic generation of collagen. We employed a clinical MPM tomograph (MPTflex, JenLab, Germany) to image vitiligo and adjacent normal areas in 10 patients undergoing treatment. The treatment consisted of either UVB light therapy or skin micro-grafting treatment followed by UVB light therapy. We visualized pigment producing melanocytes near hair follicles, migrating melanocytes within the human epidermis, newly pigmented keratinocytes in the basal layer, and epidermal melanin granules. The overarching goal is to use this technology to better define the phenotypic characteristics of migrating melanocytes in the hope of improving transplantation therapies for vitiligo.","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131647227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhenguo Wu, Liwei Jiang, Wenbo Wang, Jianhua Zhao, H. Lui, H. Zeng
{"title":"In vivo tissue micro-Raman spectroscopy with simultaneous reflectance confocal microscopy monitoring using a single laser\u0000 (Conference Presentation)","authors":"Zhenguo Wu, Liwei Jiang, Wenbo Wang, Jianhua Zhao, H. Lui, H. Zeng","doi":"10.1117/12.2513719","DOIUrl":"https://doi.org/10.1117/12.2513719","url":null,"abstract":"","PeriodicalId":131448,"journal":{"name":"Photonics in Dermatology and Plastic Surgery 2019","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130107679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}