Iranian Journal of Pediatric Hematology & Oncology最新文献

{"title":"The Blood Group Rhc Protein from Human Erythrocyte Membranes as an Immunogen for Producing Antibodies in Mice","authors":"Hadi Rezaeeyan, Fatemeh Yari, Saiedeh Milani","doi":"10.18502/ijpho.v14i1.14663","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14663","url":null,"abstract":"Background: Various methods have been used to isolate red blood cell (RBC) membrane antigens. In this regard, obtaining the antigen and preserving its structure is of special importance. However, limited studies have been conducted to purify cellular membrane antigens such as Rh proteins. \u0000Materials and Methods: In this experimental study, Rhc antigens of the RBC membrane was purified. Here, the RBC membrane was solubilized through the lysis buffer. Next, dialysis and affinity chromatography were performed using polyclonal anti-human RhCcEe antibody to isolate Rhc/e antigens from the RBCs with the following blood group characteristics: Rhc+, RhC-, Rhe+, and RhE-. The purified proteins were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dot blot methods. The immunization process was performed in Balb/c mice using the Rhc antigen as an immunogen. After the last injection, the mouse serum was used to titrate antibodies. \u0000Results: Protein bands of the purified antigen were observed in the silver-stained SDS-PAGE gel (region of 25-35 kDa). The OD405nm = 0.56 ± 0.05 results showed the reactivity with Rhc antibody. The specificity of the purified protein was evaluated using the dot blot assay. The anti-sera titration was greater than 1/10,000 against Rhc-coated microwells. Rh antigens can be isolated from the RBC membrane using the non-ionic NP-40 detergent and affinity chromatography. \u0000Conclusion: The Rh antigen can be isolated from the RBC membrane with proper purity by solubilization with the non-ionic NP-40 detergent and purification by affinity chromatography. It seems that the membrane antigen maintains its antigenicity and structure. As a result, it can be detected by blood group-specific antibodies used in the hemagglutination method. Purified antigens may be used to generate antibodies or to study the protein structure.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology & Oncology","volume":"24 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139441343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of CD34-Positive Cells in Children with B-Cell Acute Lymphoblastic Leukemia: A Case-Control Study","authors":"Mohammad Naderisorki, Hossein Karami, Mobin Ghazaiean, Iman Naseh, Hadi Darvishi Khezri, Hossein Asgarian Omran","doi":"10.18502/ijpho.v14i1.14660","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14660","url":null,"abstract":"Background: Acute lymphoblastic leukemia (ALL) is the most common cancer among children. The prognostic significance of the cluster of differentiation 34 (CD34) markers in children with B-cell acute lymphoblastic leukemia (B-ALL) is not yet fully understood. \u0000Materials and Methods: This study is a case-control trial based on the clinical data of 40 children with B-ALL who referred to a pediatric oncology center in the city of Sari, Iran. The data were derived from the demographic findings, laboratory test results at diagnosis, immunophenotyping, transfusion of blood products including packed red blood cells and platelet concentrates, and the frequency and duration of hospitalization due to febrile infection. \u0000Results: Of the participants, 42.5% were CD34-negative and 57.5% were CD34-positive. The mean age of the patients at diagnosis was 3.1 ± 3.3 years (Range:0.1-13.3 years). Also, 60.9% of the CD34-positive children and 47.1% of the CD34-negative ones were boys (P = 0.38). According to the calculated Cohen's d, the relationship of CD34 positivity with transfused packed red blood cell and platelet concentrates was mild -0.15 (95% CI -0.78 to 0.47) (P = 0.55) and moderate 0.49 (95% CI -0.15 to 1.12) (P = 0.29), respectively, which was significant in neither case. Moreover, the relationship of CD34 positivity with the hospitalization frequency of -0.51 (95% CI -1.14 to 0.13) (P = 0.22) and the hospitalization duration of -0.52 (95% CI -1.16 to 0.12) (P = 0.27) due to febrile infection was moderate to strong. \u0000Conclusion: The CD34-positive children with B-ALL experienced less blood products transfusion (except packed red blood cells) and febrile infection in terms of both the frequency and duration of hospitalization during chemotherapy. Therefore, CD34 expression in the B-ALL children was associated with better prognosis.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology & Oncology","volume":"5 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139444261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Epidemiological Study of Children with Malignant Disorders in the Pediatric Department at Menoufia University Hospital, Menoufia, Egypt during the Last Fifteen Years","authors":"S. Ragab, M. A. El-Hawy, Asmaa A. Mahmoud, Aliaa Rabie Bayomi, Mai El-Sayad Abd El-Hamid","doi":"10.18502/ijpho.v14i1.14659","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14659","url":null,"abstract":"Background: This study aims to assess the epidemiological, clinical, and paraclinical characteristics and survival of childhood with malignant disorders in the pediatrics department, menoufia University Hospital. \u0000Methods: A retrospective study with clinical and epidemiological data from patients was conducted on 314 children who attended Pediatric Department, Haematology-Oncology Unit, Menoufia University Hospital during the last fifteen years. \u0000Results: 314 children were assessed, their ages ranged from 2 months-18 years with mean 5.96±3.79 years. Also, 252 (80.3%) were diagnosed with hematological malignancies, and 62 (19.5%) were diagnosed with solid tumors. Among hematological malignancies, 186 were diagnosed with acute leukemia, 158 (49.7%) with acute lymphoblastic leukemia (ALL), and 28 (8.8%) with acute myeloid leukemia (AML). The most frequent clinical presentations were fever in 95.24% in hematological malignancies vs 48.4% in solid (p<0.001),, pallor in 92.5% in hematological malignancies vs 69.4% in solid (p<0.001), hepatomegaly in 81.3% in hematological malignancies vs 37.1% in solid (p<0.001),, lymphadenopathy in 77.6 % in hematological malignancies vs 24.2% in solid (p<0.001), and splenomegaly in 76.3% of hematological malignancies vs 12.9% in solid (p<0.001),The majority of the patients 64.15% had white blood cells (WBCs) less than 50,000/mm³, while 35.85% had WBCs more or equal to 50,000/mm³ with significant relation with risk stratification (p=0.001). The survivors who finished their treatment course were 31.8% and the recurence patients were 9%. \u0000Conclusion: Acute lymphoblastic leukemia is the most frequent childhood hematological neoplasm. Various clinical and laboratory features present at the time of initial diagnosis can predict the likelihood that a patient will remain in remission or not including age: under 1 and over 10 years, gender: male sex, WBCS more than 50,000/mm³ at presentation.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"49 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139442150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors of Second Malignant Neoplasms in Childhood Cancer Survivors","authors":"M. Bahrami, E. Sheikhpour, Mojtaba Karami, Sanaz Mehrabani","doi":"10.18502/ijpho.v14i1.14665","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14665","url":null,"abstract":"Cancer is as the second leading cause of death among children in the United States. The mortality rate for cancer has witnessed a decline, dropping from 6.5 per 100,000 in 1970 to 2.3 per 100,000 in 2016. Second malignant neoplasms (SMNs) represent novel primary malignancies emerging after the initial cancer diagnosis, particularly prominent as late effects of cancer therapy in children. The incidence of SMNs sees a substantial increase over time, reaching nearly 10% even a decade after the initial diagnosis. A comparative analysis between the general population and child cancer survivors reveals a six-fold higher risk of developing SMNs among the latter. Various factors contribute to the elevated risk of second cancers, with age, lifestyle, environmental influences, primary cancer treatment, and genetic predisposition playing pivotal roles. Noteworthy risk factors for SMNs in children encompass radiation therapy, chemotherapeutic agents, topoisomerase inhibitors, genetic factors, hematopoietic stem cell transplantation, and ionizing radiation, as elucidated in the present study. Despite these findings, further research is imperative to accurately quantify the risks associated with etiological factors, enabling the identification of individuals at a heightened risk for second cancers and facilitating proactive screening and preventive measures.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139443583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the oxidative stress status of children with ITP in two therapies using methylprednisolone and methylprednisolone along with IVIG","authors":"H. Yousefi, B. Keikhaei, Arash Alghasi, K. Jaseb, Ghorban Mohammadzadeh, Maria Cheraghi, Najmeh Nameh Goshay Fard","doi":"10.18502/ijpho.v14i1.14661","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14661","url":null,"abstract":"Background: Idiopathic thrombocytopenic purpura (ITP) is a rare and autoimmune disorder determined by an abnormal reduction in the number of platelets. The current study aims to evaluate the oxidative stress status of children with ITP in two treatment methods using methylprednisolone and methylprednisolone with intravenous immunoglobulin (IVIG). \u0000Materials and Methods: This retrospective study was conducted on 60 children with ITP who referred to Baghaei Hospital in Ahvaz in 2021.  All the ITP children were equally divided into two groups, 30 receiving methylprednisolone and 30 receiving methylprednisolone and IVIG. The sampling of the patients’ blood was done in two stages before and after the start of treatment. Then, malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant status (TAS), total oxidative status (TOS), catalase (CAT) and glutathione were measured according to the instructions in the commercial kit. The analyses were performed using SPSS software version 23. P value < 0.05 was significant. \u0000Results: The number of platelets after treatment in methylprednisolone and methylprednisolone+ IVIG groups was 133.44 ± 18.93 and 158.76 ± 34.76 (×103/µL), respectively. Itas significantly increased compared to that before the treatment (P = 0.04). The amount of TAC in the group receiving methylprednisolone + IVIG and the methylprednisolone group was 1.64 ± 0.18 and 1.26 ± 0.53 nm, respectively; there was a remarkable difference between the two groups (P = 0.001). Also, SOD, CAT and glutathione in the methylprednisolone + IVIG group were remarkably higher than those in the methylprednisolone group (P < 0.05). Finally, the levels of TOS were lower in the methylprednisolone + IVIG group (19.74 ± 9.93 μmol) than in the methylprednisolone group (26.65 ± 10.64 μmol) (P = 0.01). \u0000Conclusion: A combination of IVIG and methylprednisolone was found to have a greater effect on improving antioxidant status and decreasing the oxidative stress indices of ITP children.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"52 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139442184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Treatment Indices of Immunologic Thrombocytopenic Purpura in Pediatric Patients","authors":"Mahdieh Saberi, Mehrdad Shekiba, Alireza Jenabzade, Hadi Zare-Zardini","doi":"10.18502/ijpho.v14i1.14662","DOIUrl":"https://doi.org/10.18502/ijpho.v14i1.14662","url":null,"abstract":"Background: Immunologic Thrombocytopenic Purpura (ITP) is considered as one of the common diseases among children. The aim of this study is evaluating the treatment indices of ITP in pediatric patients. \u0000Materials and Methods: In this observational follow-up study, 123 ITP patients were assessed in term of medical history, physical examination, and laboratory tests based on the type of treatment. \u0000Results: Among 123 ITP patients, 70 (56.9%) were female and 53 (43.1%) were male with mean age of 4 years. Considering the platelet count of > 20,000, 115 (93.6%), 4 (3.3%) were treated in less than a month (acute) and 1-6 months (sub-acute). Thirty two patients (26%) did not reach the normal platelet count in 6 months (chronic). IVG, steroid, RhoGAM, steroid+ IVIG, RhoGAM + IVIG, RhoGAM+steroid+IVIG therapy was done in 10.6, 15.4, 2.4, 41.5, 4.1, and 26, respectively. Three patients did not receive any medication. There was no significant relationship between the onset of clinical symptoms and the onset of treatment based on 20,000 platelet count; however, regarding the platelet count of 150000, the relationship was statistically significant. The frequency of ITP was higher in females. There was no report on Intra-cerebral hemorrhage (ICH). In addition, 11 patients (8.9%) were provided with splenectomy. The treatment with combinational therapy of RhoGAM and IVIG was regarded as the highest treatment rate. In addition, the highest length of hospotalization based on initial treatment belonged to steroid treatment followed by the combinational therapy of steroid and IVIG. The patients receiving IVIG were the ones with the highest cost for the first 24 hours of treatment, and regarding the later hospitalization, the patients treated with steroid and combinational therapy of steroid+IVIG had to pay the highest medical expenses. \u0000Conclusion: No significant relationship between the symptoms, platelet count, and the type of treatment.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"47 35","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139442527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ORCHITIS and Chronic Granulomatous Disease","authors":"A. Yazdanparast, Gholamreza Fathpour, Arman Hashmi","doi":"10.18502/ijpho.v13i3.13134","DOIUrl":"https://doi.org/10.18502/ijpho.v13i3.13134","url":null,"abstract":"Chronic Granulomatous Disease (CGD) is a primary immunodeficiency disorder, which is almost always characterized by impairment of the function of leukocytes and generally presents with recurrent chronic relapsing bacterial or fungal infection. This study reported a three-year-old boy who was referred to the Pediatric Hematology and Oncology Center of Shohada Khalij-e-Fars general hospital, Bushehr with recurrent lymphadenitis, and orchitis, who suffered from this disease. Since confirmation of diagnosis, he is receiving Co-trimoxazole three times per week as prophylaxis, and the plan for him is hematopoietic stem cell transplantation (HSCT).","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123585995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effects of chemotherapy drugs and thiosemicarbazone complexes on the alteration of SNHG16 expression in Acute Lymphoblastic Leukemia","authors":"Fatemeh Erfanipour, Fatemeh Ghorbani, S. Mirzaahmadi, Golnaz Asaadi Tehrani","doi":"10.18502/ijpho.v13i3.13130","DOIUrl":"https://doi.org/10.18502/ijpho.v13i3.13130","url":null,"abstract":"Background: Acute lymphoblastic leukemia (ALL) is a malignant disease that afflicts both children and adults. It starts from the bone marrow (soft and spongy tissue inside the bone) where immature white blood cells or lymphocytes are formed. This study aims to compare chemotherapy drugs, methotrexate (MTX), cyclophosphamide, cytarabine (Ara-C) and mercaptopurine (6mp) with nickel (Ni) and copper (Cu) complexes of thiosemicarbazone (TSCZ) in terms of their effects on the changes of Small Nucleolar RNA Host Gene 16(SNHG16) long non coding RNA (lncRNA) expression in the ALL cell line. \u0000Materials and Methods: this experimental study was conducted on various concentrations of chemotherapy drugs including MTX, CP, Ara-C, 6mp and Ni and Cu complexes of TSCZ. The Jurkat E6.1 cell lines were subjected to passage in different groups and times (24, 48, and 72h) and then treated with the chemotherapy drugs. After RNA extraction and cDNA synthesis, the SNHG16 gene expression was evaluated by Real-Time PCR, and the results were analyzed by the Rest software. \u0000Results: As the results indicated, within 24h, MTX (0.77, 0.72), CP (0.61, 0.7), ara-C (0.78, 0.87), Cu (0.91, 0.95) and Ni (0.94, 0.79) complexes as well as complex 1(0.73) and 2 (0.94) decreased the expression of SNHG16 gene significantly (P<0.001). Furthermore within 48h, under the influence of CP (0.86, 0.89) and   Ara-C (0.94, 0.97) as well as Cu (0.96) and Ni (0.98) complexes, the gene expression continued to decline(P<0.001).The greatest effect of chemotherapy drugs belonged to the combination of 1μM of ara-C and 5μM of 6mp.(P<0.001).       \u0000Conclusion: It was found that gene expression analysis is a feasible method to identify the pathways affected by the standard induction chemotherapy in ALL patients. This finding promotes the development of novel targeted drugs and biomarkers to categorize disease aggressiveness and evaluate treatment responses.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127784664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient Production of Platelets from Umbilical Cord Blood Stem Cells Using A Collagen-Tragacanth Scaffold in A Multi-Chamber Bioreactor","authors":"Mohamad Hosein Derakhti Gonbad, A. Khoshfetrat, Akbar Movassaghpuor, Z. Sanaat, H. N. Charoudeh","doi":"10.18502/ijpho.v13i3.13131","DOIUrl":"https://doi.org/10.18502/ijpho.v13i3.13131","url":null,"abstract":"Background: To date, platelet (PLT) transfusion is the main medical option for the treatment of thrombocytopenia. Nowadays, allogenic PLTs are commonly used for blood donation. Due to the limitations in the preparation and storage of PLTs, researchers try to develop alternative modalities such as bioreactors for the in-vitro expansion and production of PLTs for therapeutic purposes by promoting the differentiation of different types of stem cells into megakaryocytes (MK) and PLTs. \u0000Materials and Methods: In this experimental study, umbilical cord blood stem cells (UCB-SC) were differentiated into MK. To this end, a new bioreactor system consisting of six compartments and a two-layer scaffold made of collagen and natural tragacanth gum was developed to mimic a bone marrow-like structure. After MKs were loaded to the top layer of the scaffold, the production rate of PLTs was analyzed under shear stress. \u0000Results: Based on the estimation, each MK produced about 17.42 PLTs loaded into the scaffold collagen. This value emerged to be 23.46 and 9.44 PLTs in the collagen-tragacanth and the pure tragacanth scaffold groups, respectively, the increase of PLTs in the collagen-tragacanth scaffold was statistically significant (p < 0.001).The generated PLTs had a positive response in aggregometry as well as interaction with normal PLTs. \u0000Conclusion: Taken together, the designed bioreactor with a collagen-tragacanth substrate can be used for the production of a sufficient number of PLTs for clinical purposes.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123199830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron Profile in Adolescent Scavengers Living in Slum Areas","authors":"Murti Andriastuti, Anisa Dwi Fathinasari, Khansa Salsabila, Miranti Fristy Medyatama","doi":"10.18502/ijpho.v13i3.13132","DOIUrl":"https://doi.org/10.18502/ijpho.v13i3.13132","url":null,"abstract":"Background: Iron deficiency anemia (IDA) is one of the major health issues in the world, especially in developing countries. During adolescence, iron deficiency can be caused by a growth spurt, inadequate nutritional intake, parasite infection, and heavy blood loss during menstruation. Regarding the importance of this issue, we aimed to assess the iron profile in adolescent scavengers living in slum areas. \u0000Materials and Methods: This was a cross-sectional study conducted in October 2016 at an alternative school for adolescents working as scavengers in Bekasi, Indonesia. Data on menstrual status, weight and height measurements, and blood samples were collected to define iron status (iron depletion, iron deficiency, and IDA). \u0000Results: In this study, 96 adolescents aged 10–18 years were recruited. The prevalence of anemia was 13.6%, and half was caused by iron deficiency. The iron profiles of subjects were iron depletion (2.1%), iron deficiency (18.8%), and IDA (7.3%). Hemoglobin, ferritin, and transferrin saturation were significantly lower in females (P<0.01, P=0.01, P<0.01 respectively). \u0000Conclusion: Anemia, iron depletion, iron deficiency, and IDA are more prevalent among adolescent girls. Special attention is needed to improve the iron status of girls, especially by giving iron supplementation for IDA prevention. Moreover, achieving the optimal iron reserve is imperative to enter a safe and healthy pregnancy by reducing delivery complications due to inadequate iron storage of both mother and fetus.","PeriodicalId":129489,"journal":{"name":"Iranian Journal of Pediatric Hematology &amp; Oncology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115167246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}