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Tentative method of analysis for suspended particulate matter in the atmosphere: (the integrating nephelometer). 大气中悬浮颗粒物的初步分析方法:(积分浊度计)。
Health laboratory science Pub Date : 1977-01-01
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引用次数: 0
Evidence against gastrointestinal pseudoparasitism by the land planarian, Bipalium kewense Moseley 1878. 陆地涡虫(Bipalium kewense Moseley, 1878)对胃肠道伪寄生虫的证据。
Health laboratory science Pub Date : 1977-01-01
J J Daly, H M Matthews, H E Farris
{"title":"Evidence against gastrointestinal pseudoparasitism by the land planarian, Bipalium kewense Moseley 1878.","authors":"J J Daly,&nbsp;H M Matthews,&nbsp;H E Farris","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The possibility of gastrointestinal pseudoparasitism by the free-living land planarian, Bipalium kewense, was tested by feeding and survival experiments. The intestinal tracts of three dogs were negative for B. kewense after the individual dogs were fed eight, three, and four large worms and autopsied at 12 h, 3 h, and 45 min, respectively. Survival of the worms in Warburg flasks, under N2, AT 27 C, was 2 h or less.. In Gas Pak jars (CO2 + H2) at 37 C, survival was less than 60 min. Aerobically, at 37 C, survival varied from 45 to 60 min. Attra-tion of the worms to stool material was examined by placing planaria inside square whose boundries were constructed of fecal smears. Bipalium kewense exposed to canine feces showed strong avoidance reactions. Urea, a nitrogen end product of these worms, was also shown to be a negative stimulus for B. kewense. Failure to establish even short term passage in the digestive tract, lethality of 37 C and anaerobic environments, and sensitivity to feces makes gastrointestinal pseudoparasitism unlikely in these organisms.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"39-43"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12019706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laboratory confirmation of an outbreak of Clostridium perfringens food poisoning. 实验室确认爆发产气荚膜梭菌食物中毒。
Health laboratory science Pub Date : 1977-01-01
D A Schiemann
{"title":"Laboratory confirmation of an outbreak of Clostridium perfringens food poisoning.","authors":"D A Schiemann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A foodborne outbreak in a group of eleven individuals sharing a common meal, eight of whom reported symptoms and an incubation period compatible with Clostridium perfringens food poisoning, was confirmed by the following laboratory criteria: (a) the C. perfringens count in the suspect food, chicken pie, was at least 10(5) per gram; (b) the median fecal C. perfringens spore count in convalescing patients was greater than 10(6) per gram: (c) an indentical serotype (PS63) was isolated from both the food and fecal specimens; and (d) the C. perfringens isolates from most of the patients (8 out of 10) were of the same serotype. The results confirm the usefulness of fecal spore counts and serotyping in implicating C perfringens in food poisoning outbreaks.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"35-8"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11405753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of enteric identification systems. 肠道识别系统的比较。
Health laboratory science Pub Date : 1977-01-01
K A Borchardt, J Gibson
{"title":"Comparison of enteric identification systems.","authors":"K A Borchardt,&nbsp;J Gibson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An evaluation of methods for identification of Enterobacteriaceae was made employing the new commercial Micro-Media Enteric System (MMES) with that of the Analytab Products Incorporated (API) and the Conventional tube media schema as suggested by the Center for Disease Control (CDS). The MMES system employed 20 biochemical tests, the API 21, and the CDC procedure 25. Sixteen of these were identical biochemical tests. Two hundred clinical isolates of Enterobacteriaceae were tested employing procedures recommended by the manufacturers of MMES and API, and methods suggested by CDC. Among the sixteen identical biochemical tests the agreement was 98.0% (Conventional), 98.2% (API), and 97.98% (MMES). Bacteria misidentified by the API system totaled 5 (2.5%), 12 (6%) for the Conventional, and 13 (6.5%) for the MMES. Five of the bacteria misidentified with the MMES procedure were due to false positive citrate tests. This problem was subsequently eliminated. The results of this study indicated that the new MMES method for identification of Enterobacteriaceae compared favorably with both the API and Conventional procedures. However, significant advantages of the MMES method were evident in initial purchase price, utilization of technology time, and less tedium performing the test.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11532418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neisseria gonorrhoeae identification in carbohydrate medium containing guinea pig serum. 含豚鼠血清的碳水化合物培养基中淋病奈瑟菌的鉴定。
Health laboratory science Pub Date : 1977-01-01
A C Maniar
{"title":"Neisseria gonorrhoeae identification in carbohydrate medium containing guinea pig serum.","authors":"A C Maniar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Clinical isolates of Neisseria gonorrhoeae from Manitoba were tested for their carbohydrate degradation activity in Cystine Trypticase Agar (C.T.A) medium, Mueller-Hinton Agar and guinea pig serum agar. Each isolate was tested using 2 carbohydrates (e.g. glucose and maltose) in the above three media. Out of 661 isolates tested, only 80% were positively identified in C.T.A. medium. Mueller-Hinton agar allowed 88% identification while guinea pig serum agar yielded 100% identification. In a second series of experiments, 102 cultures of N. gonorrhoeae were used to compare Flynn & Waitkins medium with guinea pig serum agar. Only 91 of these were identified with Flynn and Waitkins medium while guinea pig serum agar identified all the 102 isolates. Guinea pig serum provides adequate growth of fastidious N. gonorrhoeae essential for detecting specific enzymes. Since guinea pig serum does not contain maltase activity, it does not interfere with the biochemical activities tested. Guinea pig serum agar is easy to prepare, does not require a heavy inoculum and gives definite color change in the medium.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"26-9"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11609091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arsenic, selenium and antimony in urine and air. Analytical method by hydride generation and atomic absorption spectroscopy. 尿液和空气中的砷，硒和锑。氢化物发生和原子吸收光谱分析方法。
Health laboratory science Pub Date : 1977-01-01
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引用次数: 0
N-nitrosodimethylamine an ambient air. Analytical method. n -亚硝基二甲胺。分析方法。
Health laboratory science Pub Date : 1977-01-01
{"title":"N-nitrosodimethylamine an ambient air. Analytical method.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"59-63"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12019710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experience with a microscopic screening program for sputum specimens. 有痰标本显微筛选程序的经验。
Health laboratory science Pub Date : 1977-01-01
R K Porschen
{"title":"Experience with a microscopic screening program for sputum specimens.","authors":"R K Porschen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microscopic examination of almost 2,000 sputum specimens was performed over a 3 1/2-month period. The specimens were categorized into 6 groups based on numbers of leukocytes (PMN's) and squamous epithelial cells (SEC's) observed at low magnification (X 100). Bacteriologic evaluation of specimens consisted of identification and antimicrobial susceptibility tests of potential pathogens when growth was equal or predominant to the growth of oropharyngeal flora. Specimens containing greater than 25 PMN's and less than 25 SEC's had potential pathogens identified in 51% of the samples. Specimen containing greater than 25 SEC's and any number of PMN's showed a rate on only 19%. Specimens containing less than 25 PMN's and SEC's had a rate of 13%. Since initiating the screening program in which sputum specimens containing greater than 25 SEC's were unacceptable for culture, the per cent of acceptable specimens received by the laboratory nearly doubled.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"17-21"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12019705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sulfuric acid aerosol in air by titration--mixed indicator. 用滴定法测定空气中的硫酸气溶胶——混合指示剂。
Health laboratory science Pub Date : 1977-01-01
{"title":"Sulfuric acid aerosol in air by titration--mixed indicator.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"50-2"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12019707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significant bacteriuria among the mentally retarded. Identification and antibiotic sensitivity of isolates. 智力迟钝者中有明显的细菌。分离株的鉴定及抗生素敏感性。
Health laboratory science Pub Date : 1977-01-01
J W Kusek, J S Ortiz
{"title":"Significant bacteriuria among the mentally retarded. Identification and antibiotic sensitivity of isolates.","authors":"J W Kusek,&nbsp;J S Ortiz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study was carried out to determine the prevalence of significant bacteriuria in a mentally retarded population. With 100,000 or more bacteria per ml. of urine used as a criterion for significant bacteriuria, 24 of 665 persons studied were considered to have significant bacteriuria for an overall prevalence rate of 3.6%. Seventeen of the 24 positive urine cultures were either from clean catch or catheter specimens. All patients undergoing catheterization were found to have significant bacteriuria. The individual prevalence rates of significant bacteriuria for males and females were 1.7 and 5.6 respectively. In females, an increasing prevalence of bacteriuria was found primarily in the 55-64 age group. The predominant bacteria isolated from bacteriuric patients were gram-negative bacilli, Escherichia coli, being isolated in 50% of all positive cultures. Antiobiotic sensitivity patterns of the isolates indicated a high degree of in vitro resistance to both ampicillin and cephalothin.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"14 1","pages":"11-6"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11532488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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