B31. IMMUNE CELLS AND INFLAMMATORY PATHWAYS DRIVING LUNG INJURY AND INFECTION - VIEWS FROM THE BENCH最新文献

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The Effect of Febuxostat in Murine Model of LPS-Induced Lung Injury 非布司他对lps致小鼠肺损伤模型的影响
B31. IMMUNE CELLS AND INFLAMMATORY PATHWAYS DRIVING LUNG INJURY AND INFECTION - VIEWS FROM THE BENCH Pub Date : 2022-05-01 DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2596
S. Ohta, H. Ikeda, T. Ebato, H. Inoue, T. Homma, S. Suzuki, A. Tanaka, H. Sagara
{"title":"The Effect of Febuxostat in Murine Model of LPS-Induced Lung Injury","authors":"S. Ohta, H. Ikeda, T. Ebato, H. Inoue, T. Homma, S. Suzuki, A. Tanaka, H. Sagara","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2596","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2596","url":null,"abstract":"Rationale: In severe COVID-19 patients, decrease of ATP production caused by mitochondrial dysfunction thought to induce the lung injury. Febuxostat, which is a therapeutic medicine for hyperuricemia, is thought to have the effect of improving mitochondrial dysfunction and enhances the production of ATP. The purpose of this study is to investigate the effect of febuxostat in LPS induced lung injury mouse model. Methods: C57BL/6 WT mice (8-12 wk-old males) were exposed to lipopolysaccharide (LPS) intratracheally to develop the murine model of LPS-induced lung injury. For the treatment, 100 μg of febuxostat was administered twice a day from 2 days before the exposure of LPS. Bronchial lavage fluid (BALF) and lung tissue were collected 24 hours and 7 days after the LPS exposure. The BALF were analyzed for total and differential cell counts. The lung tissues were stained with Masson's trichrome staining and analyzed for lung fibrosis. Results: Twenty-four hours after the LPS exposure, the number of total cells and neutrophils in the BALF was increased. In the group receiving febuxostat, the number of total cells and neutrophils were significantly decreased at 24 hours after the LPS exposure. At 7 days after the LPS exposure, the number of total cells and neutrophils in both LPS and LPS + Febuxostat group returned to almost the same level as control group. Additionally, the percentage of collagen deposition area representing lung fibrosis in the entire lung field was enhanced in LPS group compared to control group at 7 days after the LPS exposure. Moreover, the treatment of febuxostat inhibited the fibrosis in LPS group. Conclusions: Administration of febuxostat inhibited the lung inflammation in the acute phase and improved the lung fibrosis in LPS-induced lung injury model. This study suggests that the treatment of febuxostat may inhibit the lung injury caused in severe COVID-19 patients.","PeriodicalId":126856,"journal":{"name":"B31. IMMUNE CELLS AND INFLAMMATORY PATHWAYS DRIVING LUNG INJURY AND INFECTION - VIEWS FROM THE BENCH","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128772428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of Endothelial CXCR2 in Neutrophil Recruitment into Central Nervous System in LPS Induced Septic Encephalopathy 内皮细胞CXCR2在LPS诱导脓毒性脑病中性粒细胞向中枢神经系统募集中的作用
B31. IMMUNE CELLS AND INFLAMMATORY PATHWAYS DRIVING LUNG INJURY AND INFECTION - VIEWS FROM THE BENCH Pub Date : 2022-05-01 DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2589
Y. Han, Y. Dong, H. Wang, F. Wu
{"title":"Role of Endothelial CXCR2 in Neutrophil Recruitment into Central Nervous System in LPS Induced Septic Encephalopathy","authors":"Y. Han, Y. Dong, H. Wang, F. Wu","doi":"10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2589","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a2589","url":null,"abstract":"","PeriodicalId":126856,"journal":{"name":"B31. IMMUNE CELLS AND INFLAMMATORY PATHWAYS DRIVING LUNG INJURY AND INFECTION - VIEWS FROM THE BENCH","volume":"35 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129674551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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