Gamete Research最新文献_第3页

Evidence for a binding site on the sperm plasma membrane which recognizes the murine zona pellucida: A binding site on the sperm plasma membrane 精子质膜上识别小鼠透明带的结合位点的证据:精子质膜上的一个结合位点

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140307

G. R. Poirier, R. Robinson, R. Richardson, K. Hinds, D. Clayton

引用次数: 37

The ovulation and activation of primary and secondary oocytes in LT/Sv strain mice LT/Sv株小鼠初级和次级卵母细胞的排卵和活化

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140309

M. Kaufman, S. Howlett

{"title":"The ovulation and activation of primary and secondary oocytes in LT/Sv strain mice","authors":"M. Kaufman, S. Howlett","doi":"10.1002/MRD.1120140309","DOIUrl":"https://doi.org/10.1002/MRD.1120140309","url":null,"abstract":"Eggs were isolated from the oviducts or ovaries of LT/Sv strain mice in order to investigate the pathways taken by them following spontaneous or induced parthenogenetic activation. The chromosome preparations from the ovarian oocytes that matured in vitro to metaphase I were all morphologically normal. Of 42 recently ovulated eggs that failed to activate parthenogenetically in culture, 57% on nuclear densitometric analysis were found to have the normal 2C amount of DNA, and 1N (haploid) number of chromosomes present, and were arrested at metaphase II. Somewhat unexpectedly, 43% had a 4C amount of DNA, and 2N (diploid) number of chromosomes present, had been arrested at metaphase I, and were evidently ovulated as primary oocytes. Following parthenogenetic activation, the majority of oocytes extruded a polar body and developed a single pronucleus. The activated eggs could be divided into two sub-populations according to the diameter (and therefore volume) of the pronucleus—in one group this was about one-third greater than in the other. The chromosome constitution of the two groups was determined separately at the first cleavage mitosis. Those with a normal-sized pronucleus were invariably haploid, while those with an enlarged pronuclear volume were invariably found to be diploid. The chromosomes in the diploid spreads often appeared to be associated in homologous pairs. We conclude that almost uniquely in LT/Sv strain females eggs may be activated parthenogenetically at either stage of meiotic maturation giving rise to diploid or haploid embryos, respectively.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"1 1","pages":"255-264"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90291528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Difference of fertilizing capacity between testicular sperm and vas deferens sperm in Cynops pyrrhogaster 独眼龙睾丸精子与输精管精子受精率的差异

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140304

M. Matsuda

引用次数: 3

The kinetics of the first wave of spermatogenesis in the newborn male mouse 新生雄鼠第一波精子发生的动力学

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140308

W. Sung, M. Komatsu, G. Jagiello

引用次数: 13

Induction of the acrosome reaction in guinea pig spermatozoa by calmodulin antagonist W-7 钙调素拮抗剂W-7对豚鼠精子顶体反应的诱导作用

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140303

T. Nagae, P. N. Srivastava

引用次数: 8

Technique for the long-term storage of lobster (Homarus) spermatophores 龙虾精囊的长期保存技术

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140302

Tamako Ishida, P. Talbot, Marcia J. Kooda-Cisco

{"title":"Technique for the long-term storage of lobster (Homarus) spermatophores","authors":"Tamako Ishida, P. Talbot, Marcia J. Kooda-Cisco","doi":"10.1002/MRD.1120140302","DOIUrl":"https://doi.org/10.1002/MRD.1120140302","url":null,"abstract":"We have developed a procedure for the long-term storage of lobster (Homarus) sperm. Sperm are collected from males by electrically stimulating the area around the gonopore. They are transferred with a bamboo stick to a plastic test tube containing paraffin oil and are stored for variable periods of time at 4–7°C. Sperm stored up to 289 days appear morphologically normal (equivalent to unstored sperm) when examined by phase contrast microscopy and electron microscopy, and morphologically normal sperm are able to undergo acrosome reactions. After longer periods of storage, degenerative changes begin to develop in sperm. These include loss of the nuclear spikes, condensation of the subacrosomal material, and distortion of the acrosome. Sperm stored better in spermatophores that had thick walls than in those with thin walls. In some spermatophores, bacteria were found in the sperm mass after storage; in general, sperm in these spermatophores were morphologically abnormal. This technique provides a means for saving lobster sperm for subsequent use in experiments or for artificial insemination of female lobsters. It may be adaptable to other invertebrate species that produce spermatophores.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"71 1","pages":"183-195"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86152418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

ESTIMATION AND MANIPULATION OF GLUTATHIONE LEVELS IN PREPUBERAL MOUSE OVARIES AND OVA: RELEVANCE TO SPERM NUCLEUS TRANSFORMATION IN THE FERTILIZED EGG 青春期前小鼠卵巢和卵子中谷胱甘肽水平的估计和控制:与受精卵中精子核转化的相关性

Gamete Research Pub Date : 1986-07-01 DOI: 10.1002/MRD.1120140310

H. Calvin, K. Grosshans, E. J. Blake

{"title":"ESTIMATION AND MANIPULATION OF GLUTATHIONE LEVELS IN PREPUBERAL MOUSE OVARIES AND OVA: RELEVANCE TO SPERM NUCLEUS TRANSFORMATION IN THE FERTILIZED EGG","authors":"H. Calvin, K. Grosshans, E. J. Blake","doi":"10.1002/MRD.1120140310","DOIUrl":"https://doi.org/10.1002/MRD.1120140310","url":null,"abstract":"Glutathione (GSH), the major low-molecular-weight thiol in mammalian cells, is believed to be a necessary factor for the transformation of the disulfide-stabilized sperm nucleus into the male pronucleus after fertilization. Its concentration in mouse ova, isolated from the ampulla of the oviduct after hormone-induced superovulation of 3–4-week-old mice, has been determined by an enzymic cycling microassay. The level found was 1.80 pmol per ovum. Mean ovum diameter was estimated as 71–72 μm, indicating a GSH concentration of 9–10 mM in the mouse egg. Administration of L-buthionine S, R-sulfoximine, an inhibitor of GSH biosynthesis, during the 2 days preceding ovulation, reduced ovum GSH content below 0.20 pmol (<1.0 mM). The mean GSH concentration of the hormone-stimulated ovaries was reduced from 3.2 mM to 0.2 mM under these conditions. \u0000 \u0000It has also been demonstrated that measurement and manipulation of ovum and ovarian levels of GSH can aid in studying its function in ovaries, ova, and early embryos. Hormone-induced superovulation was achieved in BSO-treated prepuberal C57B1/6 X SJL mice whose ovaries contained less than 10% of control levels of GSH. Over 50% of the isolated ova were fertilized in vitro. However, abnormal one-cell embryos resulted in which the maternally derived pronucleus coexisted with an unchanged sperm nucleus, thus confirming that adequate levels of GSH are necessary for initiating transformation of the fertilizing sperm nucleus.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"2011 1","pages":"265-275"},"PeriodicalIF":0.0,"publicationDate":"1986-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87952438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 160

Sperm-egg interactions in the shrimp Rhynchocinetes typus 斑纹虾精卵相互作用

Gamete Research Pub Date : 1986-06-01 DOI: 10.1002/MRD.1120140208

C. Barros, E. Dupré, L. Viveros

{"title":"Sperm-egg interactions in the shrimp Rhynchocinetes typus","authors":"C. Barros, E. Dupré, L. Viveros","doi":"10.1002/MRD.1120140208","DOIUrl":"https://doi.org/10.1002/MRD.1120140208","url":null,"abstract":"Sperm-egg interaction in Rhynchocinetes typus was studied with the phase-contrast and scanning electron microscopes. R typus spermatozoa present in the vas deferens have the shape of a round-headed nail. After contact with seawater it is possible to observe the unfolding of the rays or stellate arms, giving the spermatozoon the appearance of an inverted umbrella. From the center of the flat face of the umbrella emerges a spike with longitudinal striations. Ovarian eggs and spermatozoa were mixed in vitro by agitating them for two minutes in Millipore-filtered seawater. The first gamete contact was established by the spermatozoon through the tip of the spike, which exerted a lytic action on the egg envelopes. After the rigid spike was completely inside the egg, the rays became aligned parallel to each other and began to enter the eggs. Toward the final stages of ray entry, it was possible to observe fusion of the ray membranes with one another, and later the fusion process continued toward the tip of the radial spines. Concomitantly, the egg surface that surrounds the sperm swelled in a circular fashion and formed a fertilization cone. After the spermatozoon entry was complete, a scarlike mark appeared at the place on the egg surface through which penetration occurred. The whole penetration process was completed within 45-60 minutes.","PeriodicalId":12668,"journal":{"name":"Gamete Research","volume":"40 1","pages":"171-180"},"PeriodicalIF":0.0,"publicationDate":"1986-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74462571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Hamster oocyte fertilizability after 4°C storage 4℃贮藏后仓鼠卵母细胞的受精率

Gamete Research Pub Date : 1986-06-01 DOI: 10.1002/MRD.1120140206

C. Barros, E. Herrera, I. Fuenzalida, B. Argüello

引用次数: 4

Nicotinamide inhibition of Xenopus laevis oocyte maturation 烟酰胺抑制非洲爪蟾卵母细胞成熟

Gamete Research Pub Date : 1986-06-01 DOI: 10.1002/MRD.1120140207

F. Godeau, M. K. Sahni, P. Boquet, S. Koide

引用次数: 6