Frontiers in Molecular Neuroscience最新文献

{"title":"Correction: Neuroprotective effects of the pannexin-1 channel inhibitor: probenecid on spinal cord injury in rats.","authors":"Qi Qi, Xiao-Xuan Wang, Jing-Lu Li, Yu-Qing Chen, Jian-Rong Chang, Jin Xi, He-Zuo Lü, Yu-Xin Zhang","doi":"10.3389/fnmol.2025.1698001","DOIUrl":"https://doi.org/10.3389/fnmol.2025.1698001","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fnmol.2022.848185.].</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1698001"},"PeriodicalIF":3.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145280015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRP channels in epileptogenesis: calcium dysregulation mechanisms and pharmacological targeting strategies.","authors":"Guolong Deng, Dayuan Liu, Yunxiang Zhong, Muyao Wang, Baoshou Su, Hongli Jiang, Yihao Zhai, Hao Peng, Caicai Zhang, Jigao Feng","doi":"10.3389/fnmol.2025.1687359","DOIUrl":"10.3389/fnmol.2025.1687359","url":null,"abstract":"<p><p>Epilepsy, a prevalent neurological disorder affecting millions globally, manifests as recurrent synchronous neuronal discharges that disrupt normal cerebral function. Emerging evidence characterizes this condition as a network-level hyperexcitability disorder driven by aberrant neuroelectrical synchronization. At the molecular level, intracellular calcium (Ca²⁺) overload is increasingly recognized as a key contributor to seizure initiation and propagation. The regulation of neuronal Ca²⁺ homeostasis involves multiple Ca²⁺ - permeable cation channels, with transient receptor potential (TRP) channels emerging as critical mediators of pathological ion flux. These non-selective transmembrane conduits facilitate Ca²⁺ permeation and contribute to epileptogenic ionic dysregulation through subtype-specific mechanisms. Current research efforts focus on elucidating TRP channel pathophysiology across epilepsy subtypes while identifying potent pharmacological modulators. This systematic investigation of TRP channel biology and targeted therapeutic development promises to revolutionize antiepileptic drug discovery by addressing current treatment limitations in seizure prevention and disease modification. The present review synthesizes recent advances in TRP channel research and evaluates emerging strategies for therapeutic targeting in epilepsy management.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1687359"},"PeriodicalIF":3.8,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in research on propofol-induced postoperative cognitive dysfunction via Piezo channels.","authors":"Han Xue, Xiaoyu Zhang, Chenxu Chou, Yulong Jia, Chunguang Hao, Xiaguang Duan","doi":"10.3389/fnmol.2025.1668523","DOIUrl":"10.3389/fnmol.2025.1668523","url":null,"abstract":"<p><p>Postoperative cognitive dysfunction (POCD), which often affects elderly patients after anesthesia and surgery, is characterized by memory loss, trouble concentrating, and difficulties with thinking and decision-making. Propofol is a commonly used intravenous anesthetic. Its effects on the brain are complex, and researchers have been paying closer attention to them. While it can protect nerve cells in some situations, it may also cause damage. Emerging evidence suggests that mechanosensitive Piezo ion channels may serve as critical mediators. These channels allow cells to detect mechanical forces and turn them into biological signals. They may act as a link between propofol use and cognitive decline. This review highlights new findings on how propofol may affect Piezo channel function. It shows that propofol changes the physical properties of cell membranes. It makes the membranes stiffer and less fluid. These changes may change how Piezo channels react to mechanical forces. They can disturb calcium signals and synaptic function in the brain. This problem can increase inflammation and damage to mitochondria. It can weaken synaptic connections and cause cognitive decline, especially in older adults. Additionally, calcium entering through Piezo1 channels has been linked to inflammation, which may be another mechanism by which propofol and Piezo channels together cause POCD. However, clear proof of how propofol interacts with Piezo channels is still lacking. More research with molecular simulations, genetic models, and calcium imaging is needed to better understand these processes.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1668523"},"PeriodicalIF":3.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Innovative approaches in glioma therapy: exploring new therapeutic frontiers.","authors":"Pilar Marcos, Arturo Mangas, Rafael Coveñas","doi":"10.3389/fnmol.2025.1697606","DOIUrl":"https://doi.org/10.3389/fnmol.2025.1697606","url":null,"abstract":"","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1697606"},"PeriodicalIF":3.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-disease biomarker identification reveals shared diagnostic biomarkers for IVDD and NAFLD via bulk and single-cell RNA sequencing.","authors":"Jiasen Wei, Chenglong Ji, Lina Liu, Chen Yan, Linhui Han, Wenbo Lin, Ximing Xu, Kaiqiang Sun","doi":"10.3389/fnmol.2025.1639705","DOIUrl":"10.3389/fnmol.2025.1639705","url":null,"abstract":"<p><strong>Introduction: </strong>Intervertebral disc degeneration (IVDD) and non-alcoholic fatty liver disease (NAFLD) represent major global health burdens. Although recent evidence points to a potential association between these two conditions, the underlying molecular mechanisms remain poorly understood. This study aims to elucidate their shared molecular landscape using integrated bioinformatics approaches.</p><p><strong>Methods: </strong>Three IVDD and two NAFLD datasets were acquired from the Gene Expression Omnibus (GEO). We performed differential expression analysis (DEGs), weighted gene co-expression network analysis (WGCNA), and machine learning to identify shared hub genes. The diagnostic relevance of these genes was further assessed using ROC curves and nomograms. Single-cell sequencing analysis was employed to examine gene expression patterns across cell clusters in intervertebral disk and liver tissues. <i>In vivo</i> experiments were conducted to evaluate the influence of NAFLD on IVDD progression and the therapeutic potential of exercise intervention.</p><p><strong>Results: </strong>Six shared genes were identified between IVDD and NAFLD. Among these, ME1, HAS2, and ADRB2 were highlighted as potential biomarkers. Validation confirmed consistent expression patterns and strong predictive performance for both diseases. KEGG pathway and immune infiltration analyses indicated significant involvement of these biomarkers in disease-related pathways and immune cell interactions. Single-cell sequencing revealed distinct expression profiles and functional roles of ME1, HAS2, and ADRB2 across relevant cell types. <i>In vivo</i> studies demonstrated that NAFLD exacerbates IVDD progression, and intervention through swimming exercise ameliorated NAFLD and exerted protective effects on IVDD under high-fat diet conditions.</p><p><strong>Discussion: </strong>This study identifies ME1, HAS2, and ADRB2 as pivotal shared biomarkers for IVDD and NAFLD, providing new insights into their molecular interconnection. The findings enhance our understanding of the comorbid mechanisms and highlight the potential of exercise as a therapeutic strategy for both conditions. These results pave the way for further mechanistic and clinical research into common pathways and integrated treatment approaches.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1639705"},"PeriodicalIF":3.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoglin alleviates neuropathic pain by protecting the blood-spinal cord barrier through the TGF-β/Smad2 signaling pathway.","authors":"SiJing Liao, Fei-Ran Zhou, Qun Li, Tao Jin, Gui-Fang Xiang, Xin Wang, Yan Liu, Hq Zhu, Qing Liu, Yuexin Liu, Ying Zhang","doi":"10.3389/fnmol.2025.1603619","DOIUrl":"10.3389/fnmol.2025.1603619","url":null,"abstract":"<p><strong>Introduction: </strong>Central neuroinflammation is pivotal in neuropathic pain pathogenesis, with blood-spinal cord barrier (BSCB) dysfunction recognized as a trigger for neuroinflammation and pain, though molecular mechanisms remain poorly understood.</p><p><strong>Methods: </strong>Through comparative clinical studies measuring serum endoglin in postherpetic neuralgia (PHN) patients versus healthy controls, and animal investigations using spared nerve injury (SNI) rat models with recombinant endoglin intervention, we assessed mechanical/thermal hyperalgesia, microglial activation, inflammatory cytokines, BSCB permeability, and TGF-βRI/Smad2/NR2B phosphorylation.</p><p><strong>Results: </strong>PHN patients exhibited lower serum endoglin versus controls; SNI rats showed reduced spinal endoglin compared to sham controls. Recombinant endoglin alleviated hyperalgesia while reversing microglial activation, inflammation, BSCB impairment, and NR2B phosphorylation. SNI decreased spinal TGF-βRI expression and Smad2 phosphorylation.</p><p><strong>Discussion: </strong>These findings demonstrate that endoglin reduction disrupts BSCB integrity via TGF-β/Smad2 pathway inhibition in endothelial cells, driving microglial activation, neuroinflammation, and NR2B phosphorylation, thereby elucidating a key pain mechanism and identifying endoglin as a therapeutic target.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1603619"},"PeriodicalIF":3.8,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tamoxifen attenuates manganese-induced dysregulation of neuronal REST via the genomic ER-<i>α</i> mechanism.","authors":"Alexis Digman, Edward Pajarillo, Sanghoon Kim, Itunu Ajayi, Deok-Soo Son, Michael Aschner, Eunsook Lee","doi":"10.3389/fnmol.2025.1648904","DOIUrl":"10.3389/fnmol.2025.1648904","url":null,"abstract":"<p><p>Chronic exposure to elevated levels of manganese (Mn) causes a neurological disorder referred to as manganism, resembling pathological symptoms of Parkinson's disease (PD). The repressor element-1 silencing transcription factor (REST) induces neuroprotection in several neurological disorders, including PD and Mn toxicity. Tamoxifen (TX), a selective estrogen receptor modulator, has been shown to afford neuroprotective effects in various experimental models and increase REST expression via the non-genomic estrogen receptor (ER)/Wnt signaling in Cath. a-differentiated (CAD) neuronal cultures. The present study investigated whether TX enhances REST transcription through the genomic estrogen receptor (ER) pathway in CAD cells, using a combination of Western blotting, quantitative reverse transcription polymerase chain reaction (qRT-PCR), promoter activity assays, chromatin immunoprecipitation, electrophoretic mobility shift assays, and site-directed mutagenesis. The findings showed that the REST promoter sequences contained half-site estrogen response elements (ERE) motifs. The ER-<i>α</i> pathway primarily upregulated REST, as the ER-α selective agonist propylpyrazole triol (PPT) (1 μM) predominantly increased REST transcription and attenuated Mn (250 μM)-induced REST reduction in CAD cells. TX induced REST upregulation by activation of the genomic ER-<i>α</i> pathway, as it increased nuclear ER-<i>α</i>'s interaction with cyclic adenosine monophosphate (AMP) response element (CREB)-binding protein and Sp1 and promoted ER-α binding to the half-site ERE in the REST promoter. Moreover, the ERE mutation in the REST promoter reduced TX-induced REST promoter activity, and TX reversed Mn-induced REST transcriptional repression. Our novel findings suggest that the genomic ER-<i>α</i> pathway plays a critical role in TX-induced REST upregulation and mitigation of Mn-induced decreases in REST expression.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1648904"},"PeriodicalIF":3.8,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent and subnucleus-specific gene expression responses to chronic stress hormone exposure in the amygdala.","authors":"Shuhei Ueda, Manami Kakita, Masahito Hosokawa, Koji Arikawa, Kiyofumi Takahashi, Ryusuke Shiota, Masaki Kakeyama, Hiroko Matsunaga, Haruko Takeyama, Sayaka Takemoto-Kimura","doi":"10.3389/fnmol.2025.1659846","DOIUrl":"10.3389/fnmol.2025.1659846","url":null,"abstract":"<p><p>Major depressive disorder (MDD) is one of the most prevalent mental disorders, posing a significant socioeconomic burden worldwide. Its development involves both genetic and environmental factors, among which chronic stress is considered a major contributor. The amygdala, a key brain region for emotional regulation, is critically implicated in MDD pathophysiology. Given its complex subnuclear architecture, it is essential to characterize stress-induced molecular changes at the level of individual subnuclei. To investigate subnucleus-specific molecular adaptations to chronic stress, we performed RNA sequencing on fluorescence-guided micropunch samples from five amygdala-related subnuclei in mice exposed to chronic corticosterone (CORT): the basolateral amygdala (BLA), the lateral and medial central amygdala (CeL, CeM), and the oval and fusiform bed nuclei of the stria terminalis (BNSTov, BNSTfu). Comparative transcriptomic analysis revealed highly divergent and subnucleus-resolved gene expression responses to chronic CORT exposure. Each subregion exhibited unique profiles of differentially expressed genes, implicating alterations in excitatory-inhibitory synaptic balance, glial functions involving oligodendrocytes or astrocytes, and neuropeptide signaling. Our results uncover the molecular heterogeneity of subnucleus-specific responses within the amygdala. These findings highlight the importance of anatomically resolved analyses in elucidating the biological basis of stress-related mental disorders such as MDD, thereby paving the way for more targeted therapeutic strategies.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1659846"},"PeriodicalIF":3.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotection against beta-amyloid toxicity by the novel estrogen receptor modulator STX requires convergent signaling pathways.","authors":"Hun-Joo Lee, Zoe Bostick, John Doherty, Tracy L Swanson, Martin J Kelly, Joseph F Quinn, Nora E Gray, Philip F Copenhaver","doi":"10.3389/fnmol.2025.1670646","DOIUrl":"10.3389/fnmol.2025.1670646","url":null,"abstract":"<p><strong>Introduction: </strong>STX is a synthetic non-steroidal estrogen receptor modulator (SERM) that can provide many of the beneficial effects of 17β-estradiol in the brain without its adverse side effects, via its selective engagement of the membrane estrogen receptor GqMER. Using both neuronal culture assays and transgenic mouse models of Alzheimer's disease (AD), we have shown that STX protects against the deleterious effects of <i>β</i>-amyloid (Aβ), in part by supporting mitochondrial function and synaptic integrity. However, the specific transduction pathways by which STX induces these beneficial responses have not been previously investigated.</p><p><strong>Methods: </strong>Using the MC65 neuroblastoma model of Aβ toxicity and primary cultures of hippocampal neurons from the 5XFAD mouse model of AD, we analyzed the involvement of different signal transduction pathways associated with STX-dependent responses in other contexts. We used pharmacological methods to test the role of key pathway components in assays of cell viability, neuronal morphology, quantitative immunoblots to analyze pathway engagement, and modulation of the mitochondrial permeability transition pore.</p><p><strong>Results: </strong>We found that the neuroprotective effects of STX against Aβ toxicity required engagement of the PI3K/Akt/GSK3β pathway. Using well-characterized inhibitors of specific isoforms of the p110 catalytic domain of PI3K, we then showed that this response was predominantly mediated via engagement of the P110δ isoform, with a more modest contribution by P110β. In contrast, targeting the PLC/PKC/PKA pathway (which plays a prominent role in hypothalamic neurons) had a relatively modest effect on the neuroprotective responses induced by STX, while targeting ERK/MAPK signaling had no significant effect.</p><p><strong>Discussion: </strong>In combination with our previous studies, these results indicate that engagement of GqMER by STX promotes neuroprotective responses via convergent signaling pathways that mitigate the effects of Aβ toxicity on mitochondrial function, synaptic integrity, and neuronal calcium (Ca²⁺) homeostasis. They also provide the framework for testing the mechanisms of STX neuroprotection <i>in vivo</i>, using mouse AD models. Since STX has been shown to provide many of the beneficial effects of 17β-estradiol in the brain without its adverse side effects (including feminizing effects in males), these results support the hypothesis that STX might have therapeutic potential in patients at risk of AD.</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1670646"},"PeriodicalIF":3.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12464056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: S-9-PAHSA ameliorates cognitive decline in a type 2 diabetes mouse model by inhibiting oxidative stress and apoptosis via CAIII modulation.","authors":"Xin-Ru Wang, Shan-Shan Huang, Meng Wang, Jin-Hong Lin, Jian-Tao Wang, Jiao-Qi Ren, Cheng-Feng He, Wen-Jiao Xue, Yin Wang, Xue-Chun Wang, Yan-Li Zhang, Ji-Chang Xiao, Jing-Chun Guo, Hou-Guang Zhou","doi":"10.3389/fnmol.2025.1688881","DOIUrl":"https://doi.org/10.3389/fnmol.2025.1688881","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fnmol.2025.1617543.].</p>","PeriodicalId":12630,"journal":{"name":"Frontiers in Molecular Neuroscience","volume":"18 ","pages":"1688881"},"PeriodicalIF":3.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}