Free Radicals and Antioxidants最新文献_第9页

Total Phenolic, Flavonoid and Alkaloid Contents, Oxidative DNA Damage Protective and Antioxidant Properties of Methanol and Aqueous Extracts of Dissotis rotundifolia Whole Plant 圆叶甜草全株甲醇和水提物总酚、类黄酮和生物碱含量、DNA氧化损伤保护及抗氧化性能

Free Radicals and Antioxidants Pub Date : 2018-07-09 DOI: 10.5530/fra.2018.2.13

W. Darkwah, Y. Ao, M. Adinortey, A. Weremfo, F. Abrokwah, E. Afriyie

引用次数: 14

Nitric Oxide and Inhibition of PPAR Gamma Regulate the ANG II induced VSMC Proliferation: Relationship with Gi Protein Levels 一氧化氮和抑制PPAR γ调节ANG II诱导的VSMC增殖:与Gi蛋白水平的关系

Free Radicals and Antioxidants Pub Date : 2017-04-08 DOI: 10.29011/2574-7711.100028

Lama Hamadeh, Marcel Bassil

{"title":"Nitric Oxide and Inhibition of PPAR Gamma Regulate the ANG II induced VSMC Proliferation: Relationship with Gi Protein Levels","authors":"Lama Hamadeh, Marcel Bassil","doi":"10.29011/2574-7711.100028","DOIUrl":"https://doi.org/10.29011/2574-7711.100028","url":null,"abstract":"Cardiovascular diseases are, nowadays, viewed as the major cause of morbidity and mortality. They are due to complications from hypertension, thrombosis, atherogenesis, and Rest enosis. In these pathologies, increased Vascular Smooth Muscle cells (VSMCs) hypertrophy, migration and proliferation promote their onset and progression. Furthermore, Nitric Oxide (NO), a vasoprotective molecule was shown to decrease the levels of inhibitory G alpha-protein (Giα) in VSMCs, thus indicating their possible involvement in its anti-proliferative effects. Peroxisome Proliferator Activated Receptors gamma (PPAR-γ) is linked to both NO and G-proteins. In this study, the aim was to establish that the anti-proliferative role of NO in VSMCs is mediated by decreased Giα while investigating the role of PPAR-γ as a modulator of these effects. For these purposes, A-10 cells and VSMCs were incubated with the NO donor Sodium Nitro Prusside (SNP) and/or the PPAR-γ antagonist GW9962 (20 μM and 30 μM). Cellular proliferation was assessed by a cell proliferation assay, and Giα and PPAR-γ expression were assessed by western blotting. Results show that SNP and GW9962 decreased the expression of Giα-2 and Giα-3 by 50% with no difference between individual and combination treatments. In addition, SNP had no effect on PPAR-γ activation while GW9962 increased PPAR-γ inactivation by 30-70% with combination treatments having no effect. Furthermore, SNP and/or GW9962 following stimulation with ANG II showed that they decrease the proliferation of VSMCs by21.57%, 23.33%, and 27.54 % while combination treatments do not indicate an interaction between PPAR-γ and NO. In conclusion, these results show that NO and GW9962 may independently inhibit VSMCs proliferation and decrease Giα protein levels.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84789760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical Analysis of Cinnamomum zeylanicum Bark and Molecular Docking of Procyanidin B2 against the Transcription Factor Nf- κB 肉桂树皮的植物化学分析及原花青素B2对转录因子Nf- κB的分子对接

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.30

B. Varalakshmi, V. Anand, T. Karpagam, A. Shanmugapriya, S. Gomathi, J. Sugunabai, V. Suganya, S. Geetha, S. Sathianachiyar

{"title":"Phytochemical Analysis of Cinnamomum zeylanicum Bark and Molecular Docking of Procyanidin B2 against the Transcription Factor Nf- κB","authors":"B. Varalakshmi, V. Anand, T. Karpagam, A. Shanmugapriya, S. Gomathi, J. Sugunabai, V. Suganya, S. Geetha, S. Sathianachiyar","doi":"10.5530/FRA.2017.2.30","DOIUrl":"https://doi.org/10.5530/FRA.2017.2.30","url":null,"abstract":"Introduction: Identification of novel natural antioxidant compounds is a highly demanding avenue of therapy for oxidative stress induced diseases. The bark of Cinnamomum zeylanicum commonly known as Ceylon cinnamon is commonly used in Ayurvedic medicine. Method: In this study, the methanolic extract of bark was subjected to GC-MS, UV absorption and TLC techniques to analyze the presence and to elucidate the structure of proanthocyanidins present in the bark. In the latter part of the study, chemdraw structure of the identified procyanidin B2 was subjected to in silico molecular drug docking analysis using GOLD to find out its inhibiting efficacy against NF-κB (1NFI). Results: The phytochemical analysis supported the presence of a proanthocyanidin compound, procyanidin B2. The constitutive or aberrant activation of the transcription factor, NF-κB pathway is often noticed in many cancers, autoimmune disorders, pulmonary, cardiovascular, neurodegenerative and skin diseases. The docking of procyanidin B2 with NF-κB revealed its inhibiting efficacy by binding to active site of NF-κB and thus could inhibited the nuclear translocation and DNA binding of p50/p65 heterodimer to κB DNA sequences. Conclusions: Thus, procyanidin B2 can act as the inhibitor for NF-κB. So, procyanidin B2 present in C. zeylanicum bark can be used as a potential lead compound for drug development against cancer and other oxidative stress disorders.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"50 1","pages":"195-199"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90279701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The role of N-acetylcysteine amide in defending primary human retinal pigment epithelial cells against tert-butyl hydroperoxide- induced oxidative stress n -乙酰半胱氨酸酰胺在保护原代人视网膜色素上皮细胞免受叔丁基过氧化氢诱导的氧化应激中的作用

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.26

Hsiu-Jen Wang, Yue-Wern Huang, Shakila Tobwala, A. Pfaff, R. Aronstam, N. Ercal

{"title":"The role of N-acetylcysteine amide in defending primary human retinal pigment epithelial cells against tert-butyl hydroperoxide- induced oxidative stress","authors":"Hsiu-Jen Wang, Yue-Wern Huang, Shakila Tobwala, A. Pfaff, R. Aronstam, N. Ercal","doi":"10.5530/FRA.2017.2.26","DOIUrl":"https://doi.org/10.5530/FRA.2017.2.26","url":null,"abstract":"Background: Age-related macular degeneration (AMD) is a leading cause of blindness in the United States among adults age 60 and older. While oxidative stress is implicated in the pathogenesis of AMD, dietary antioxidants have been shown to delay AMD progression in clinical studies. We hypothesized that N-acetylcysteine amide (NACA), a thiol antioxidant, would protect retinal pigment epithelium and impede progression of retinal degeneration. Methods: tert-Butyl hydroperoxide (TBHP) was used to induce oxidative stress in cell cultures. The goal was to evaluate the efficacy of NACA in an in vitro model of AMD in primary human retinal pigment epithelial cells (HRPEpiC). Results: Our data indicates that TBHP generated reactive oxygen species (ROS), which reduced cell viability, depleted glutathione (GSH) levels, and compromised glutathione reductase (GR) activity. Pretreatment with NACA significantly reduced ROS generation, restored GSH levels and GR activity, and recovered transepithelial electrical resistance. Pretreatment with NACA did not decrease the number of dying cells as determined by flow cytometry analysis. However, survival was significantly improved when cells were co-exposed to NACA and TBHP after a shortened pretreatment period. Conclusion: Our data suggest that pretreatment with NACA reduces sublethal but not lethal effects of TBHP in HRPEpiC. NACA significantly improves cell survival when administered prior to and during oxidative damage similar to that observed in the development of dry AMD. These results indicate that continuation of a thiol antioxidant regimen for treatment of AMD is beneficial throughout the course of the disease, and NACA is a potent antioxidant that should be further evaluated for this purpose.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"42 1","pages":"172-177"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90753648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antioxidant Potential of a Toxic Plant Calotropis procera R.Br. 有毒植物原花椒的抗氧化潜力。

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.21

Mruthunjaya Kenganoral, M. Bhaskaran, M. Santhepete, V. I. Hukkeri

{"title":"Antioxidant Potential of a Toxic Plant Calotropis procera R.Br.","authors":"Mruthunjaya Kenganoral, M. Bhaskaran, M. Santhepete, V. I. Hukkeri","doi":"10.5530/FRA.2017.2.21","DOIUrl":"https://doi.org/10.5530/FRA.2017.2.21","url":null,"abstract":"Background: Calotropis procera R.Br. (Family, Apocynaceae) is a toxic herb which was known to have great importance in the Ayurveda. The plant is anthelmintic; relieves strangury; cures ulcers; the ashes act as an expectorant. The plant was also known to be used in the treatment of jaundice from ancient times. Studies have shown that it has cardio-protective property in Myocardial Infarction. The present study was focused on evaluating the anti-oxidant activity of the leaves by in vitro models. Methods: The anti-oxidant activity in different extracts and fractions of the leaves viz. total alcoholic and water extracts; petroleum ether, ethyl acetate, butanol and water fractions were evaluated for antioxidant properties using various in vitro models like free radical scavenging activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, hydrogen peroxide scavenging activity, reduction of ferric ions and lipid peroxidation assay; and total antioxidant capacity was established. Results: Antioxidant potency was found to be highest in butanol fraction, water fraction and aqueous alcoholic extract. Conclusions: The antioxidant property of different extracts and fractions of the leaves were studied in vitro and results have shown that they are potent antioxidant in nature.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"95 1","pages":"143-151"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90822621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Safety Profile and Effect on Libido of a Combined Bryophyllum pinnatum, Moringa oleifera and Vitamin C Phytotherapeutic Agent 苔藓、辣木和维生素C复合植物治疗剂的安全性及对性欲的影响

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.23

A. Sparman, K. Thompson

{"title":"Safety Profile and Effect on Libido of a Combined Bryophyllum pinnatum, Moringa oleifera and Vitamin C Phytotherapeutic Agent","authors":"A. Sparman, K. Thompson","doi":"10.5530/FRA.2017.2.23","DOIUrl":"https://doi.org/10.5530/FRA.2017.2.23","url":null,"abstract":"Introduction: Both Moringa oleifera and Bryophyllum pinnatum are well known phytotherapeutics with a range of potential applications that capitalize on their anti-oxidant properties, ranging from improving risk factors for cardiovascular disease to increasing sexual desire. The literature, however, mainly focuses on these effects in-vitro or in non-human subjects. Aims: We aimed to investigate the side effects of a combination agent with known proportions of these two phytotherapeutics, as well as to determine any effect on the human sexual drive. Method: Sixty nine (n=69) participants were enrolled in a prospective cohort study and followed up for a minimum of six months. Data regarding adverse effects and libido was determined from a 22-item Quality of Life questionnaire as well as a checklist of common side-effects completed at each monthly follow-up. Results: Eight participants were lost to follow-up; in the remaining participants there were no reported major adverse effects. No abnormal bleeding, urinary tract infections, asthma exacerbations, or changes in memory were reported. Minor issues reported by participants taking the agent included fluctuations in appetite (22.9%) and sleep pattern (16.4%), gastrointestinal upset (14.7%), respiratory symptoms, such as shortness of breath or wheezing (8.1%), and muscle aches (8.1%). Women did not report a change in libido whereas 31.8% of men reported a statistically significant increase in libido by their second to third follow up (p<0.05). Conclusion: This study acts as a small preliminary report and suggests that combination M. oleifera, B. pinnatum and vitamin C has a favorable safety profile and may increase libido in human males.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"9 1","pages":"156-159"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82231630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In vitro Anti-oxidant Activity in Methanolic Extracts of five Pogostemon Spciece 五种广藿香乙醇提取物的体外抗氧化活性研究

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/fra.2017.2.29

M. Kaliyappan, Nagarajan Nallasamy, Siva Priya Kathirivan Thirumuruthy

引用次数: 2

Preliminary Outcomes on the Use of an Antioxidant Dietary Supplement for Patients with or at Risk of Heart Disease 心脏病患者或有心脏病风险的患者使用抗氧化剂膳食补充剂的初步结果

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.22

A. Sparman

{"title":"Preliminary Outcomes on the Use of an Antioxidant Dietary Supplement for Patients with or at Risk of Heart Disease","authors":"A. Sparman","doi":"10.5530/FRA.2017.2.22","DOIUrl":"https://doi.org/10.5530/FRA.2017.2.22","url":null,"abstract":"Aims: To report initial feasibility outcomes from a pilot study on the use of a potent antioxidant dietary supplement on several parameters in persons with or at risk of heart disease. Methods and Material: In this uncontrolled longitudinal pilot study, sixty-six participants received a dietary supplement consisting of Moringa oleifera, Bryophyllum pinnatum and vitamin C. Participants were instructed to consume one capsule daily for a period of six months. Once a month, blood work and a quality of life questionnaire were completed and the data recorded. Feasibility was based on the researcher’s observations and collected data. Statistical analysis used: Due to the nature of the study no statistical packages were used. Excel spreadsheets and measures of location were used to analyze the data. Results: Recruitment and retention data was indicative of feasibility. With 37.9% of the registered participants being lost to follow-up. A 3.26% change in diastolic blood pressure was noted among female participants one month after their initial blood pressure was recorded. Blood glucose levels decreased among participants by 1.81% after three months of supplement use. High Density Lipoprotein (HDL) cholesterol levels increased for both groups, with the males experiencing a 9.25% increase in their HDL levels. On the other hand, Low Density Lipoprotein cholesterol levels among female participants decreased by 5.6%. Conclusions: The pilot data is supportive of the implementation of a randomized, long-term evidence-based intervention.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"6 1","pages":"152-155"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79544689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Investigation of Antioxidant Activity and Phytochemical Compositions of Celtis Tournefortii 荆刺草抗氧化活性及植物化学成分研究

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.24

I. Yıldırım, Y. Uğur, T. Kutlu

引用次数: 10

Antioxidant Activities of Root, Stem and Leaves of Vernonia cinerea (L) Less. 水桐根、茎、叶的抗氧化活性研究(L)

Free Radicals and Antioxidants Pub Date : 2017-03-15 DOI: 10.5530/FRA.2017.2.27

A. Goggi, N. Malpathak

引用次数: 10