Fermentation Technology最新文献_第5页

A Novel Microbial Aldehyde Oxidase Applicable to Production of Useful Raw Materials, Glycolic Acid and Glyoxylic Acid, from Ethylene Glycol 一种适用于从乙二醇生产有用原料乙醇酸和乙醛酸的新型微生物醛氧化酶

Fermentation Technology Pub Date : 2015-08-09 DOI: 10.4172/2167-7972.1000116

Miwa Yamada, K. Isobe

{"title":"A Novel Microbial Aldehyde Oxidase Applicable to Production of Useful Raw Materials, Glycolic Acid and Glyoxylic Acid, from Ethylene Glycol","authors":"Miwa Yamada, K. Isobe","doi":"10.4172/2167-7972.1000116","DOIUrl":"https://doi.org/10.4172/2167-7972.1000116","url":null,"abstract":"Glycolic acid is an attractive raw material which is used as a dyeing and tanning agent in the textile industry, a flavoring agent and preservative in the food processing industry, and a skin care agent in the pharmaceutical industry. It is also utilized for the production of polyglycolic acid and other biocompatible copolymers. Glycolic acid can be isolated from natural sources, such as sugarcane, sugar beets, pineapple, or cantaloupe, but it is also chemically synthesized by hydrogenation of oxalic acid with nascent hydrogen or the hydrolysis of the cyanohydrin derived from formaldehyde. Ethylene glycol is a relatively inexpensive starting material for the production of glycolic acid by an oxidation reaction. However, the chemical oxidation reaction of ethylene glycol has certain drawbacks, such as the formation of formaldehyde and other compounds as by-products. To overcome such drawbacks of chemical synthesis for the production of glycolic acid, one of the preferred methods is to use enzymatic production rather than chemical synthesis. The utilization of microbial enzymes also has the major advantage of promoting simple and eco-friendly industrial-scale production. We therefore designed a new enzymatic method for the production of glycolic acid from ethylene glycol using two microbial oxidases; ethylene glycol is first converted to glycolaldehyde by an ethylene glycol-oxidizing enzyme, and the resulting glycolaldehyde is then oxidized to glycolic acid by an aldehyde oxidase (ALOD) (Figure 1).","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83211672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Relationship between Interfacial Hydrophobicity and Hydroxylation Activity ofFungal Cells Located on an Organic Aqueous Interface 有机水界面真菌细胞的界面疏水性与羟基化活性的关系

Fermentation Technology Pub Date : 2015-05-18 DOI: 10.4172/2167-7972.1000E122

S. Oda

{"title":"Relationship between Interfacial Hydrophobicity and Hydroxylation Activity ofFungal Cells Located on an Organic Aqueous Interface","authors":"S. Oda","doi":"10.4172/2167-7972.1000E122","DOIUrl":"https://doi.org/10.4172/2167-7972.1000E122","url":null,"abstract":"A Liquid–liquid interface bioreactor (L–L IBR), which consists of a hydrophobic organic solvent (an upper phase), a layer of fungal cellballooned polyacrylonitrile microsphere (diameter, 20-40 μm; density, 0.03-0.20; a middle phase), and a liquid medium (a lower phase), is a unique and effective cultivation system for the microbial transformation with fungi [1]. The system has some interesting and practically important characteristics, i.e., alleviation of toxicity of poisonous substrate and/or product solubilized in the organic phase, excellent productivity of valuable hydrophobic chemicals, efficient supply of oxygen from the organic phase to fungal cells, control and management of pH and nutrients in the liquid medium, depression of catabolize repression caused by easily metabolizable carbon sources [2], and easy recovery of product without troublesome solvent extraction. The system has been applied to various microbial reactions, such as hydrolysis of an acetate ester [1,3], asymmetric reduction of an aromatic dike tone [4], and regio- and stereo selective epoxidation of - caryophyllene to (–)-β-caryophyllene oxide so far [5]. In all cases, it has been observed that substrate concentration, product accumulation, and region- and stereo selectivity’s of reaction reached very high level compared with two traditional cultivation systems, submerged and organic–aqueous two-liquid-phase systems.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"19 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2015-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84085837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impact of Different Plant Secondary Metabolites Addition: Saponin, Tannic Acid, Salicin and Aloin on Glucose Anaerobic Co-Digestion 不同植物次生代谢产物添加:皂苷、单宁酸、水杨苷和芦荟苷对葡萄糖厌氧共消化的影响

Fermentation Technology Pub Date : 2015-04-08 DOI: 10.4172/2167-7972.1000113

Philippe Mambanzulua Ngoma, S. Hiligsmann, Eric Sumbu Zola, M. Ongena, P. Thonart

{"title":"Impact of Different Plant Secondary Metabolites Addition: Saponin, Tannic Acid, Salicin and Aloin on Glucose Anaerobic Co-Digestion","authors":"Philippe Mambanzulua Ngoma, S. Hiligsmann, Eric Sumbu Zola, M. Ongena, P. Thonart","doi":"10.4172/2167-7972.1000113","DOIUrl":"https://doi.org/10.4172/2167-7972.1000113","url":null,"abstract":"Vegetal waste and some wastewater of agro-food industries contain plant secondary metabolites (PSMs). It was showed in nutritional researches that these substances such as saponins and tannins reduced the methane production in the rumen. To our knowledge no study was done in the waste treatment domain to evaluate the inhibitory effect of the principal glycosidic metabolites from the wastewater or vegetal waste on their own methaneproducing anaerobic digestion. Therefore in this paper BMP tests were carried out at 30°C with four commercial PSMs (CPSMs) in mixture with glucose monohydrate (Gl) used as control sample. These CPSMs were saponin from Quilaja saponaria Molina Pract (Sap), tannic acid (Tan), salicin (Sal) and aloin from Curacao Aloe (Alo) representing respectively saponins, tannins, alcoholic glycosides and anthraquinones sources. Acidogenesis and acetogenesis were recorded for all the mixtures of Gl and CPSMs; however their conversion rates decreased with the increase of the concentrations of CPSMs. By contrast, the methanogenesis was inhibited at concentrations of CPSMs above 0.3 g/l. The inhibition degree for aromatic compounds on the anaerobic biodegradation of Gl seemed directly to depend on the numbers of benzene rings in the medium and the synergism. Thus, the highest inhibition of the biogas production from Gl was recorded for Alo, followed by Sap, Tan and Sal. However, the highest inhibition of the methane production from Gl was recorded with Sap, Alo, Tan and Sal. It was supposed that the toxicity potentials of these PSMs on their own biomethanization would be in following decreasing order: Sap or Alo, Tan and Sal. Therefore, the concentration of PSMs alone or in mixture in a digester should be below 0.3 g/l for a better methanization.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"67 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2015-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74554086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Immobilization of Selected Microbes at Some Selected Solid Supports for Enhanced Fermentation Process 特定微生物在特定固体载体上的固定化强化发酵过程

Fermentation Technology Pub Date : 2015-04-08 DOI: 10.4172/2167-7972.1000115

R. Hewawasam, C. Udawatte, S. Weliwegamage, S. Sotheeswaran, S. Rajapakse

引用次数: 4

Production and Partial Characterization of Lipase from Pseudomonas putida 恶臭假单胞菌脂肪酶的制备及部分特性研究

Fermentation Technology Pub Date : 2015-01-01 DOI: 10.4172/2167-7972.1000112

Huda Fatima, N. Khan, A. Rehman, Z. Hussain

引用次数: 13

Coupling Proteomics and Fermentation Technology for the Improvement of Bioactive Molecule Production Yield in Actinomycetes 提高放线菌生物活性分子产率的蛋白质组学与发酵耦合技术

Fermentation Technology Pub Date : 2013-12-17 DOI: 10.4172/2167-7972.1000E118

G. Gallo

{"title":"Coupling Proteomics and Fermentation Technology for the Improvement of Bioactive Molecule Production Yield in Actinomycetes","authors":"G. Gallo","doi":"10.4172/2167-7972.1000E118","DOIUrl":"https://doi.org/10.4172/2167-7972.1000E118","url":null,"abstract":"Copyright: © 2013 Gallo. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Most bioactive molecules (like anticancers, antitumors, antibiotics, immunosuppressants, insecticidals, antivirals, herbicidals, antifungals) with valuable industrial and market value are naturally produced by actinomycetes [1-4], Gram-positive filamentous bacteria widespread in both terrestrial and aquatic environments [5,6]. Out of thousands of bioactive molecules, also known as secondary metabolites since they are not essential for actinomycete growth in standard laboratory condition at least, more than 50% are synthesised by strains of Streptomyces genus [4]. Despite the cellular and ecological role of secondary metabolites is still debated [3,7], microbial fermentation is widely exploited to produce these compounds at industrial level. Although they have many different activities and range within a vast chemical complexity and diversity, there are two main common issues which could be addressed for the establishment of a cost-effective microbial fermentation process:","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"159 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2013-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76753943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mushrooms-The Incredible Factory for Enzymes and Metabolites Productions 蘑菇——不可思议的酶和代谢物生产工厂

Fermentation Technology Pub Date : 2013-09-16 DOI: 10.4172/2167-7972.1000E117

M. Camassola

引用次数: 9

Process and Strain Development for Reduction of Broth Viscosity with Improved Yield in Coenzyme Q10 Fermentation by Agrobacterium tumefaciens ATCC 4452 农杆菌ATCC 4452降低辅酶Q10发酵液粘度及提高产量的工艺及菌株研究

Fermentation Technology Pub Date : 2013-03-16 DOI: 10.4172/2167-7972.1000110

Pradipta Tokdar, A. Wani, Pratyush Kumar, Prafull V. Ranadive, S. George

{"title":"Process and Strain Development for Reduction of Broth Viscosity with Improved Yield in Coenzyme Q10 Fermentation by Agrobacterium tumefaciens ATCC 4452","authors":"Pradipta Tokdar, A. Wani, Pratyush Kumar, Prafull V. Ranadive, S. George","doi":"10.4172/2167-7972.1000110","DOIUrl":"https://doi.org/10.4172/2167-7972.1000110","url":null,"abstract":"Viscous nature of the fermentation broth has phenomenal influence on process conditions and parameters in a fermentor. Though broth rheology has attracted significant influence in process research, still there is a challenge to modify fluid dynamics of fermentation broth. During the production of coenzymeQ10 (CoQ10) by Agrobacterium tumefaciens ATCC 4452, the culture broth becomes highly viscous due to excessive synthesis of exopolysaccharides. This hinders the CoQ10 yield and complicates the downstream process. The present study describes how this problem was tackled by media modification and mutation. Induced mutants were generated using UV and EMS as mutagenic agents followed by rational selection based on antibiotic resistance. On screening of these mutants in sucrose based PM-2 medium, UV induced, vancomycin resistant mutant M-6, showed significant reduction (6.29 fold) in viscosity development in the broth. Mutant M-6(S), a natural variant of mutant M-6, resistant to high substrate concentration was further selected for the CoQ10 production. Cane molasses as carbon source was found to be best suitable for CoQ10 fermentation using mutant M-6(S). Replacing sucrose with cheaper cane molasses significantly reduced the broth viscosity with improved specific CoQ10 content, thereby generating cost effective fermentation process. The newly developed mutant strain produced 48.89 mg/L of CoQ10 with specific CoQ10 content of 1.87 mg/g of DCW at 25°C, 500 rpm agitation and 0.2 vvm aeration using continuous fed batch fermentation and newly formulated cane molasses medium.","PeriodicalId":12351,"journal":{"name":"Fermentation Technology","volume":"47 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78396680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Plackett-Burman Design: A Statistical Method for the Optimization of Fermentation Process for the Yeast Saccharomyces cerevisiae Isolated from the Flowers of Woodfordia fruticosa Plackett-Burman设计:一种统计方法优化从木福特花中分离的酿酒酵母菌发酵工艺

Fermentation Technology Pub Date : 2013-01-01 DOI: 10.4172/2167-7972.1000109

J. Manwar, K. Mahadik, A. Paradkar

引用次数: 21

Fermented Foods and Obesity 发酵食品与肥胖

Fermentation Technology Pub Date : 2012-12-08 DOI: 10.4172/2167-7972.1000E116

P. O. Uvere

引用次数: 1