Fisheries and Aquatic Sciences最新文献_第7页

Functional characterization and expression analysis of c-type and g-like-type lysozymes in yellowtail clownfish (Amphiprion clarkii) 克氏Amphiprion clarkii黄尾小丑鱼c型和g型溶菌酶的功能表征及表达分析

Fisheries and Aquatic Sciences Pub Date : 2023-03-01 DOI: 10.47853/fas.2023.e16

Gaeun Kim, H. Sohn, Wkm Omeka, Chaehyeon Lim, Don Anushka Sandaruwan Elvitigala, Jehee Lee

{"title":"Functional characterization and expression analysis of c-type and\u0000 g-like-type lysozymes in yellowtail clownfish (Amphiprion\u0000 clarkii)","authors":"Gaeun Kim, H. Sohn, Wkm Omeka, Chaehyeon Lim, Don Anushka Sandaruwan Elvitigala, Jehee Lee","doi":"10.47853/fas.2023.e16","DOIUrl":"https://doi.org/10.47853/fas.2023.e16","url":null,"abstract":"Lysozymes are well-known antibacterial enzymes that mainly target the peptidoglycan layer of the bacterial cell wall. Animal ly-sozymes are mainly categorized as g-type, c-type, and i-type based on protein sequence and structural differences. In this study, c-type (AcLysC) and g-like-type (AcLysG-like) lysozymes from Amphiprion clarkii were characterized in silico via expressional and functional approaches. According to in silico analysis, open reading frames of AcLysC and AcLysG-like were 429 bp and 570 bp, respectively, encoding the corresponding polypeptide chains with 142 and 189 amino acids. Elevated expression levels of AcLysC and AcLysG-like were observed in the liver and the heart tissues, respectively, as evidenced by quantitative real-time polymerase chain reaction assays. AcLysC and AcLysG-like transcript levels were upregulated in gills, head kidney, and blood cells following experimental immune stimulation. Recombinant AcLysC exhibited potent lytic activity against Vibrio anguillarum , whereas recombinant AcLysG-like showed remarkable antibacterial activity against Vibrio harveyi and Streptococcus parauberis , which was further evidenced by scanning electron microscopic imaging of destructed bacterial cell walls. The findings of this study collectively suggest the potential roles of AcLysC and AcLysG-like in host immune defense.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89002002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infection of Enteromyxum leei in cultured starry flounder Platichthys stellatus 养殖星形比目鱼lei肠杆菌的感染

Fisheries and Aquatic Sciences Pub Date : 2023-03-01 DOI: 10.47853/fas.2023.e20

S. Shin, Jehee Lee

引用次数: 0

Surimi for snacks: physicochemical and sensory properties of fried fish snacks prepared from surimi of different fish species 小吃用鱼糜:用不同鱼种的鱼糜制作的炸鱼小吃的理化和感官特性

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e12

Yunjin Choi, J. Chae, Seonghui Kim, Eui‐Cheol Shin, Gibeom Choi, Duhyeon Kim, Suengmok Cho

引用次数: 0

Change in plasma cortisol and glucose levels of Oncorhynchus keta according to water temperature 血皮质醇和葡萄糖水平随水温的变化

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e10

Young-Seok Seo, H. Lee, Joo Hak Jeong, S. Mun, Han-Kyu Lim

引用次数: 0

Apparent digestibility coefficients of plant feed ingredients for olive flounder (Paralichthys olivaceus) 橄榄比目鱼植物饲料原料的表观消化率系数

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e7

Md Mostafizur Rahman, Buddhi E. Gunathilaka, Sang-Guan You, Kang-Woong Kim, Sang-Min Lee

{"title":"Apparent digestibility coefficients of plant feed ingredients for olive flounder (Paralichthys olivaceus)","authors":"Md Mostafizur Rahman, Buddhi E. Gunathilaka, Sang-Guan You, Kang-Woong Kim, Sang-Min Lee","doi":"10.47853/fas.2023.e7","DOIUrl":"https://doi.org/10.47853/fas.2023.e7","url":null,"abstract":"This study was designed to determine the apparent digestibility coefficients of soybean meal, soy protein concentrate (SPC), soy protein isolate (SPI), rapeseed meal (RSM), pea protein concentrate (PPC), wheat gluten meal (WGM) and wheat flour (WF) for olive flounder, Paralichthys olivaceus. A reference diet (RF) was formulated to meet the nutrient requirements of olive flounder with 1% chromic oxide (Cr2O3) as an inert indicator. Test diets were prepared to contain 70% RF and 30% of the test ingredient. Olive flounder, averaging 150 ± 8.0 g, was cultured in 400-L fiberglass tanks at a density of 25 fish per tank. Fecal collection columns were attached to each tank. Fecal samples were obtained from triplicate groups of fish for 4 weeks. Dry matter digestibility of SPC (75%) and WGM (76%) were significantly higher than the other test ingredients. Protein digestibility of SPC (85%), PPC (88%) and WGM (89%) were significantly higher than the other test ingredients, and protein digestibility of RSM (77%) and WF (76%) was lower than the other ingredients tested. Lipid digestibility of SPC (72%) and SPI (69%) were significantly higher than the other test ingredients. Energy digestibility of SPC (85%) and WGM (82%) were significantly higher than that of others tested ingredients. The availability of amino acids in WGM was generally higher than in other plant-feed ingredients. Therefore, SPC and WGM were seems to be efficient as potential protein sources for olive flounder compared to other tested ingredients. Overall, findings of the current study may assist in more efficient and economical formulation of diets using plant feed ingredients for olive flounder.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134941984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive secondary metabolites in sea cucumbers and their potential to use in the functional food industry 海参中生物活性次生代谢物及其在功能食品工业中的应用潜力

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e6

KK Asanka Sanjeewa, Khinm Herath

{"title":"Bioactive secondary metabolites in sea cucumbers and their potential\u0000 to use in the functional food industry","authors":"KK Asanka Sanjeewa, Khinm Herath","doi":"10.47853/fas.2023.e6","DOIUrl":"https://doi.org/10.47853/fas.2023.e6","url":null,"abstract":"The bioactive secondary metabolites produced by sea cucumbers are very diverse with differences in composition, linkages, molecular weight, and various functional properties. Due to their physicochemical properties, these bioactive molecules in sea cucumbers have found applications in various market segments such as functional foods and cosmetics. Sea cucumber side dishes are a prominent food item in traditional cuisine in East Asian countries such as South Korea, China, and Japan. In addition, many studies have reported that the consumption of sea cucumbers can reduce the risk of cardiovascular disease, the pathogenesis of cancer cells, chronic inflammatory diseases, etc. In particular, many studies have recently reported the potential of sea cucumbers to develop functional products to reduce inflammation, oxidative stress, diabetes, and cancer. Additionally, these bioactive properties associated with sea cucumbers make them ideal compounds for use as functional ingredients in functional food products. However, no report has yet reviewed the properties of sea cucumbers related to functional foods. Therefore, in this review, the primary focus is given to collecting published scientific data (from 2019 to 2023) on the bioactive properties of sea cucumbers relevant to the functional food industry.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80027601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative study on antioxidant activity of Gold 1, a new strain of Pyropia yezoensis yezoensis新菌株Gold 1抗氧化活性的比较研究

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e13

J. Hyun, Sang-Woon Lee, Hyeon Hak Jeong, Jae-Il Kim

引用次数: 0

Auranofin accelerates spermidine-induced apoptosis via reactive oxygen species generation and suppression of PI3K/Akt signaling pathway in hepatocellular carcinoma 金嘌呤通过活性氧生成和抑制PI3K/Akt信号通路加速亚精胺诱导的肝癌细胞凋亡

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e11

Hyun Hwangbo, D. H. Kim, Min Yeong Kim, S. Ji, EunJin Bang, S. Hong, Yung-Hyun Choi, J. Cheong

引用次数: 1

Molecular characterization and expression of CD96 in red seabream (Pagrus major) 红鲷(Pagrus major) CD96的分子特征及表达

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e8

Won-Sik Woo, Kwang-Min Choi, Min-Soo Joo, G. Kang, K. Kim, Ha-Jeong Son, Min-Young Sohn, Do-Hyung Kim, Chan-Il Park

引用次数: 0

Factors influencing farmed fish traders’ intention to use improved fish post-harvest technologies in Kenya: application of technology acceptance model 影响肯尼亚养殖鱼贸易商使用改良鱼采后技术意愿的因素:技术接受模型的应用

Fisheries and Aquatic Sciences Pub Date : 2023-02-01 DOI: 10.47853/fas.2023.e9

J. B. Mboya, K. Obiero, M. Cheserek, Kevin O. Ouko, E. Ogello, N. Outa, Elizabeth Akinyi Nyauchi, D. Kyule, J. Munguti

引用次数: 0