Faktori eksperimental'noi evolucii organizmiv最新文献_第7页

Application of nanomaterial for maturation in vitro of cattle embryos 纳米材料在牛胚胎体外成熟中的应用

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v28.1385

P. A. Trotskyi, O. Shcherbak, S. Kovtun

{"title":"Application of nanomaterial for maturation in vitro of cattle embryos","authors":"P. A. Trotskyi, O. Shcherbak, S. Kovtun","doi":"10.7124/feeo.v28.1385","DOIUrl":"https://doi.org/10.7124/feeo.v28.1385","url":null,"abstract":"Aim. To evaluate the effectiveness of the use of nanomaterial in the environment for the further development of in vitro embryos derived from frozen-thawed oocytes in the system of conservation of genetic resources of animals at the cellular level. Methods. Biotechnological, cryobiological, morphological, cytogenetic, and statistical methods, as well as methods of statistical data processing were used in the research. Results. Oocyte-cumulus complexes (OCC) of cows were divided into four groups: three experimental, in which the maturation was performed in a medium containing 0.1, 0.01 and 0.001% UFS/sucrose and control - without the addition of nanobiomaterial. In vitro fertilization of pre-mature frozen-thawed ova of cows and subsequent maturation of embryos in the medium with the addition of UFS/sucrose (0.001%) showed an increase in the number of embryos by 16.7-22.1% compared with the addition of 0.1; 0.01% and 13.1% compared to the control group. It was found that the fragmentation rate of 2-cell cattle embryos decreased from 65.0 to 39.8% with a decrease in the concentration of UFS/sucrose from 0.1 to 0.001%. The most stable indicators of the fragmentation index from 78.4 to 50.0% were observed on the fourth day of embryo cultivation in experimental group B. Conclusions. Reducing the concentration of UFS/sucrose from 0.1 to 0.001% in the composition of the medium for in vitro maturation of cattle embryos leads to an increase of 16.7-22.1% in the number of embryos obtained. \u0000Keywords: oocyte-cumulus complex, cryopreservation, nanomaterial, in vitro maturation, embryo.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85874887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIR-HLAC genotyping in married couples with early reproductive losses of unknown genesis 起因不明的早期生殖丧失的已婚夫妇的KIR-HLAC基因分型

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1424

K. Sosnina, D. Zastavna, O. Terpyliak

{"title":"KIR-HLAC genotyping in married couples with early reproductive losses of unknown genesis","authors":"K. Sosnina, D. Zastavna, O. Terpyliak","doi":"10.7124/feeo.v29.1424","DOIUrl":"https://doi.org/10.7124/feeo.v29.1424","url":null,"abstract":"Aim. KIR-HLAC genotyping in married couples with early idiopathic pregnancy loss. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes was analyzed and the frequency of KIR genotypes in women with early reproductive losses was established. The most common (77.78 %) was the AB genotype, 20.37 % had the AA genotype, and 1.85 % had the BB genotype. HLAC genotyping of couples with regular early reproductive losses showed the C1/C2 genotype of the HLAC gene in 40.74 % of women and 44.44 % of men. The frequency of C1/C1 genotype in women was 27.78% versus 38.89 % in men. The C2/C2 genotype of the HLAC gene was detected in 31.48 % of women and 12.96 % of men. According to the results of KIR-HLAC analysis of genotyping of married couples with early reproductive losses, a high/significant risk of reproductive losses of immunological genesis was found in 55.56 % of cases. Conclusions. KIR-HLAC genotyping is a genetic test that allows to assess the risks of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy. \u0000Keywords: early reproductive losses, KIR, HLAC.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81193029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Initial developmental rate delay in modern highly productive cultivars of winter bread wheat from West Europe 西欧现代高产冬面包小麦品种的初始发育速率延迟

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1417

A. Stelmakh, N. Litvinenko, V. Fait

引用次数: 0

Longevity of genepool maize seeds under model conditions 模型条件下玉米基因库种子寿命的研究

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1411

O. Zadorozhna

引用次数: 0

Genetic transformation of promising genotypes of winter bread wheat by in planta method 冬面包小麦有前途基因型的株内遗传转化

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v28.1384

L. Slivka, O. Dubrovna

{"title":"Genetic transformation of promising genotypes of winter bread wheat by in planta method","authors":"L. Slivka, O. Dubrovna","doi":"10.7124/feeo.v28.1384","DOIUrl":"https://doi.org/10.7124/feeo.v28.1384","url":null,"abstract":"Aim. Optimization of conditions and genetic transformation of new promising genotypes of winter bread wheat (Triticum aestivum L.) by in planta method. Methods. Agrobacterium-mediated transformation by the in planta method using strain AGL0 and vector construct pBi-OAT. Results. The influence of air temperature, optical density of cells of agrobacterial suspension, inoculation day and composition of inoculation medium on the frequency of obtaining transgenic plants of new promising genotypes of winter wheat was studied. The dependence of the frequency of obtaining transgenic plants on environmental conditions, in particular temperature, has been established. It was found that the temperature regime of 20-22 °C provided the largest number (4.4%) of wheat transformants, and when the temperature is reduced to 16-18 °C there is a decrease in the efficiency of T-DNA transfer into the plant genome and the lowest frequency of transformation is observed. Conclusions. The largest number of transformants was obtained using a inoculation medium without sucrose, the optical density of cells of the agrobacterial suspension of 0.4 op.od. and inoculation on the third day after castration of ears. \u0000Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine-δ-aminotransferase gene.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"10 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83724105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of genotypic features of effects of plant treatment with growth regulators on canopy parameters and yield of winter wheat 生长调节剂处理对冬小麦冠层参数及产量影响的基因型特征分析

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1415

G. A. Priadkina, O. Stasik, N. Makharinska, I. P. Kondratskaya

{"title":"Estimation of genotypic features of effects of plant treatment with growth regulators on canopy parameters and yield of winter wheat","authors":"G. A. Priadkina, O. Stasik, N. Makharinska, I. P. Kondratskaya","doi":"10.7124/feeo.v29.1415","DOIUrl":"https://doi.org/10.7124/feeo.v29.1415","url":null,"abstract":"Aim. A study of the effects of growth regulators derived from natural raw materials on the photosynthetic apparatus surface and the yield of winter wheat. Methods. Morphometric, spectrophotometric, statistical. Results. An increase in the value of canopy chlorophyll index (ChlI) of Smuhlianka and Astarta varieties under the treatment of plants with Stimpo growth stimulator was established at anthesis, a positive effect of the regulator Regoplant – only for Smuhlianka variety at milk-wax ripeness. Chlorophyll photosynthetic potential (ChlP) during the period anthesis – milk-wax ripeness increased significantly under the use of growth regulators in both varieties, while the efficiency of treatments had varietal differences: growth was greater (11–18% compared to control) in the variety Smuhlianka than in the variety Astarta (6–7%). A positive correlation was established between the yield and ChlP during the period anthesis – milk-wax ripeness. Under the action of treatment of plants with growth regulators, the increase in grain yield was significant (0.29–0.45 t/ha) only in the variety Smuhlianka. Conclusions. The positive effect of treatments of plants with growth regulators, obtained from natural raw materials, on the value of canopy ChlI at anthesis and ChlP during period anthesis – milk-wax ripeness was established. A positive correlation between the winter wheat yield and ChlP during the period anthesis – milk-wax ripeness was found. Varietal differences in the effect of growth regulators treatment of plants on ChlP and yield were found. \u0000Keywords: Triticum aestivum L., grain productivity, chlorophyll index, chlorophyll photosynthetic potential, growth regulator.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87725470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining ability of corn inbreds – carriers of mutations Su1 and Su2 on the content of oleic acid glycerides 突变Su1和Su2的玉米自交系对油酸甘油酯含量的配合力

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1405

D. Tymchuk, G. Potapenko, N. Tymchuk, I.K. Kuznetsova

{"title":"Combining ability of corn inbreds – carriers of mutations Su1 and Su2 on the content of oleic acid glycerides","authors":"D. Tymchuk, G. Potapenko, N. Tymchuk, I.K. Kuznetsova","doi":"10.7124/feeo.v29.1405","DOIUrl":"https://doi.org/10.7124/feeo.v29.1405","url":null,"abstract":"Aim. Evaluation of donors’ properties of corn inbreds - carriers of endospermic mutations su1 and su2 on the oleates content. Methods. A series of hybrids obtained in top- crosses of 10 inbreds of the common type with four testers – low-oleic and high-oleic inbreds of the common type and inbreds – carriers of the su1 and su2 mutations were analyzed. Oleates’s content was determined by the gas chromatographic method. Results. The highest effects of the general combining ability were shown by the inbreds obtained from high-oil synthetics and the inbreds - carriers the su1 and su2 mutations. Hybrids of low-oleic inbreds with the sources of su1 and su2 mutations had an increased content of oleates in comparison with maternal forms, and hybrids of high-oleic inbreds with the sources of these mutations inherited the oleates content in an intermediate type. In F2 hybrids from crosses of inbreds of the common type with the inbreds - carriers of the su1 and su2 mutations, transgressions were observed. Conclusions. The increased content of oleates in corn inbreds inbreds and hybrids is most likely controlled by oleate - coding loci of chromosome 4 and 6 linked to mutant genes su1 and su2. The carriers of these genes can be used as sources of increased oleate content in the corn breeding for oil quality. \u0000Key words: Zea mays L., endospermic mutants, oleates content, top-crosses","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83072084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological characteristics of winter wheat varieties to the main pathogens in the right-bank Forest Steppe 右岸森林草原冬小麦品种对主要病原菌的免疫特性

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v29.1410

L. Golosna, O. Afanasieva, O. Shevchuk, L. Kucherova, I. S. Shvets, L. V. Hubenko

{"title":"Immunological characteristics of winter wheat varieties to the main pathogens in the right-bank Forest Steppe","authors":"L. Golosna, O. Afanasieva, O. Shevchuk, L. Kucherova, I. S. Shvets, L. V. Hubenko","doi":"10.7124/feeo.v29.1410","DOIUrl":"https://doi.org/10.7124/feeo.v29.1410","url":null,"abstract":"Aim. To determine the resistance of winter wheat varieties to the main pathogens, to establish their stability and plasticity, to identify perspective sources of resistance. Methods. Laboratory – production of inoculum of pathogens; field – artificial inoculation,, assessment of variety stability; statistical calculation of disease severity, indicators of stability and plasticity. Results. In 2015–2017, the resistance of 43 varieties of winter wheat to the main pathogens of leaf diseases, common bunt and root rots was assessed. Resistance to powdery mildew was found in 32 varieties, tan spot – in 2, root rot – in 3, hard smut – in 2 varieties. Six varieties of winter wheat were characterized by group resistance. Varieties that combine high plasticity and stability of the sign of disease resistance have been identified. Conclusions. Valuable sources of resistance are winter wheat varieties with group resistance to common bunt and powdery mildew – Tradytsiia Odeska and Kurs; powdery mildew and tan spot – Nasnaga and Zolotonozhka; powdery mildew and root rot – Nezabudka and Shchedrist kyivska.Keywords: resistance, winter wheat, diseases, plasticity, stability.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90057606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal fitness parameters and selection indices in the natural population of Drosophila melanogaster Meig. 黑腹果蝇自然种群的季节适应度参数和选择指数。

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v28.1367

E. V. Vakulenko, E. S. Koptevtsova, D. Grigoryev, V. Y. Strashnyuk

{"title":"Seasonal fitness parameters and selection indices in the natural population of Drosophila melanogaster Meig.","authors":"E. V. Vakulenko, E. S. Koptevtsova, D. Grigoryev, V. Y. Strashnyuk","doi":"10.7124/feeo.v28.1367","DOIUrl":"https://doi.org/10.7124/feeo.v28.1367","url":null,"abstract":"Aim. The purpose of investigation was to study the seasonal selection indices and its components in the natural population of Drosophila melanogaster Meig. Methods. The material for the study was the Gaidary-spring and Gaidary-autumn lines, obtained from the natural population of Gaidary in different seasons of the year. Fecundity and mortality/survival rates in the pre-reproductive period of development were studied. Based on these indicators, the selection indices were calculated according to the Crow`s formula. Results. In terms of individual components of fitness, such as egg production of females, the number of adult offspring, embryonic and pupal mortality, the spring and autumn populations of Gaidary did not show significant differences. Selection indices (Itot) in the autumn population of Gaidary were higher than in the spring population: in the embryonic period of development – by 50.4%, in the postembryonic period – by 67.5%. Differences in the intensity of selection are due to the variance of fertility, which was higher in the autumn population. Conclusions. Seasonal changes in the intensity of selection in different periods of ontogenesis are cumulative, which leads to increased selection pressure in the population of Gaidary in the autumn compared to spring. \u0000Keywords: Drosophila melanogaster Meig., natural population, fecundity, embryonic mortality, pupal mortality, Crow’s indices.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81289105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species-specific mobile genetic elements in the gene of repair enzyme MGMT in new world monkeys 新世界猴修复酶MGMT基因中物种特异性的可移动遗传元件

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI: 10.7124/feeo.v28.1388

O. V. Pidpala, L. Lukash

引用次数: 1