Synapse (New York, N.y.)最新文献_第3页

Environmental enrichment alters structural plasticity of the adolescent brain but does not remediate the effects of prenatal nicotine exposure. 环境富集改变了青少年大脑的结构可塑性，但不能弥补产前尼古丁暴露的影响。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2014-07-01 Epub Date: 2014-03-04 DOI: 10.1002/syn.21737

Richelle Mychasiuk, Arif Muhammad, Bryan Kolb

{"title":"Environmental enrichment alters structural plasticity of the adolescent brain but does not remediate the effects of prenatal nicotine exposure.","authors":"Richelle Mychasiuk, Arif Muhammad, Bryan Kolb","doi":"10.1002/syn.21737","DOIUrl":"https://doi.org/10.1002/syn.21737","url":null,"abstract":"Exposure to both drugs of abuse and environmental enrichment (EE) are widely studied experiences that induce large changes in dendritic morphology and synaptic connectivity. As there is an abundance of literature using EE as a treatment strategy for drug addiction, we sought to determine whether EE could remediate the effects of prenatal nicotine (PN) exposure. Using Golgi-Cox staining, we examined eighteen neuroanatomical parameters in four brain regions [medial prefrontal cortex (mPFC), orbital frontal cortex (OFC), nucleus accumben, and Par1] of Long-Evans rats. EE in adolescence dramatically altered structural plasticity in the male and female brain, modifying 60% of parameters investigated. EE normalized three parameters (OFC spine density and dendritic branching and mPFC dendritic branching) in male offspring exposed to nicotine prenatally but did not remediate any measures in female offspring. PN exposure interfered with adolescent EE-induced changes in five neuroanatomical measurements (Par1 spine density and dendritic branching in both male and female offspring, and mPFC spine density in male offspring). And in four neuroanatomical parameters examined, PN exposure and EE combined to produce additive effects [OFC spine density in females and mPFC dendritic length (apical and basilar) and branching in males]. Despite demonstrated efficacy in reversing drug addiction, EE was not able to reverse many of the PN-induced changes in neuronal morphology, indicating that modifications in neural circuitry generated in the prenatal period may be more resistant to change than those generated in the adult brain.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"293-305"},"PeriodicalIF":2.3,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21737","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40299564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Phencyclidine rapidly decreases neuronal mRNA of brain-derived neurotrophic factor. 苯环利定迅速降低脑源性神经营养因子神经元mRNA。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2014-06-01 Epub Date: 2014-02-24 DOI: 10.1002/syn.21735

Yusuke Katanuma, Tadahiro Numakawa, Naoki Adachi, Noriko Yamamoto, Yoshiko Ooshima, Haruki Odaka, Takafumi Inoue, Hiroshi Kunugi

{"title":"Phencyclidine rapidly decreases neuronal mRNA of brain-derived neurotrophic factor.","authors":"Yusuke Katanuma, Tadahiro Numakawa, Naoki Adachi, Noriko Yamamoto, Yoshiko Ooshima, Haruki Odaka, Takafumi Inoue, Hiroshi Kunugi","doi":"10.1002/syn.21735","DOIUrl":"https://doi.org/10.1002/syn.21735","url":null,"abstract":"Downregulation of brain-derived neurotrophic factor (BDNF), a member of neurotrophin family, has been implicated in psychiatric diseases including schizophrenia. However, detailed mechanisms of its reduction in patients with schizophrenia remain unclear. Here, using cultured cortical neurons, we monitored BDNF mRNA levels following acute application of phencyclidine [PCP; an N-methyl-d-aspartate (NMDA) receptor blocker], which is known to produce schizophrenia-like symptoms. We found that PCP rapidly caused a reduction in total amount of BDNF transcripts without effect on cell viability, while mRNA levels of nerve growth factor was intact. Actinomycin-D (ActD), an RNA synthesis inhibitor, decreased total BDNF mRNA levels similar to PCP, and coapplication of ActD with PCP did not show further reduction in BDNF mRNA compared with solo application of each drug. Among BDNF exons I, IV, and VI, the exon IV, which is positively regulated by neuronal activity, was highly sensitive to PCP. Furthermore, PCP inactivated cAMP response element-binding protein (CREB; a regulator of transcriptional activity of exon IV). The inactivation of CREB was also achieved by an inhibitor for Ca(2+) /calmodulin kinase II (CaMKII), although coapplication with PCP induced no further inhibition on the CREB activity. It is possible that PCP decreases BDNF transcription via blocking the NMDA receptor/CaMKII/CREB signaling.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"257-65"},"PeriodicalIF":2.3,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40296720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts. 超短波与骨髓基质细胞对脱细胞异体神经移植神经再生的协同作用。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2013-10-01 Epub Date: 2013-05-02 DOI: 10.1002/syn.21669

Chao-Jian Pang, Lei Tong, Li-Li Ji, Zhen-Yu Wang, Xu Zhang, Hai Gao, Hua Jia, Li-Xin Zhang, Xiao-Jie Tong

{"title":"Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts.","authors":"Chao-Jian Pang, Lei Tong, Li-Li Ji, Zhen-Yu Wang, Xu Zhang, Hai Gao, Hua Jia, Li-Xin Zhang, Xiao-Jie Tong","doi":"10.1002/syn.21669","DOIUrl":"https://doi.org/10.1002/syn.21669","url":null,"abstract":"Acellular nerve allografts (ANA) possess bioactivity and neurite promoting factors in nerve tissue engineering. Previously we reported that low dose ultrashort wave (USW) radiation could enhance the rate and quality of peripheral nerve regeneration with ANA repairing sciatic nerve defects. Meanwhile, ANA implanted with bone marrow stromal cells (BMSCs) exhibited a similar result. Thus, it is interesting to know whether it might yield a synergistic effect when USW radiation is combined with BMSCs-laden ANA. Here we investigated the effectiveness of ANA seeded with BMSCs, combined with USW therapy on repairing peripheral nerve injuries. Adult male Wistar rats were randomly divided into four groups: Dulbecco's modified Eagle's medium (DMEM) control group, BMSCs-laden group, ultrashort wave (USW) group and BMSC + USW group. The regenerated nerves were assayed morphologically and functionally, and growth-promoting factors in the regenerated tissues following USW administration or BMSCs integration were also detected. The results indicated that the combination therapy caused much better beneficial effects evidenced by increased myelinated nerve fiber number, myelin sheath thickness, axon diameter, sciatic function index, nerve conduction velocity, and restoration rate of tibialis anterior wet weight. Moreover, the mRNA levels of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in the spinal cord and muscles were elevated significantly. In conclusion, we found a synergistic effect of USW radiation and BMSCs treatment on peripheral nerve regeneration, which may help establish novel strategies for repairing peripheral nerve defects.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"637-47"},"PeriodicalIF":2.3,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40244980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Transient activation of mTOR following forced treadmill exercise in rats. 大鼠强迫跑步机运动后mTOR的短暂激活。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2013-09-01 Epub Date: 2013-05-21 DOI: 10.1002/syn.21668

Betina Elfving, Tina Christensen, Cecilia Ratner, Jacob Wienecke, Anders B Klein

{"title":"Transient activation of mTOR following forced treadmill exercise in rats.","authors":"Betina Elfving, Tina Christensen, Cecilia Ratner, Jacob Wienecke, Anders B Klein","doi":"10.1002/syn.21668","DOIUrl":"https://doi.org/10.1002/syn.21668","url":null,"abstract":"The beneficial effect of exercise on hippocampal plasticity is possibly mediated by increased angiogenesis and neurogenesis. In angiogenesis, insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), and hypoxia-inducible factor 1, alpha subunit (HIF1α) are important factors, while the induction of neurogenesis requires signaling through the VEGF receptor, Flk-1 (VEGFR-2). VEGF expression is believed to be regulated by two distinct mTOR (mammalian target of Rapamycin)-containing multiprotein complexes mTORC1 and mTORC2, respectively. This study was initiated to investigate the effect of exercise on the expression of VEGF, cognate receptors, HIF1α, mTORC1, and mTORC2 in hippocampus and frontal cortex. To this end, we measured messenger RNA (mRNA) levels in rat brain using quantitative real-time polymerase chain reaction (real-time qPCR) after forced treadmill exercise for 1 day, 2 weeks, and 8 weeks. Rats were euthanized either immediately (0 h) or 24 h after last exercise session. Here, we show that exercise affected mRNA levels of VEGF, VEGFR2, and the coreceptor neuropilin 2 (NRP2) when the rats were euthanized immediately, whereas at 24 h only the expression of mTOR was regulated after a single bout of exercise. In conclusion, the effect of treadmill exercise on the VEGF system is acute rather than chronic and there is a transient activation of mTOR. More studies are needed to understand whether this could be beneficial in the treatment of neuropsychiatric disorders.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"620-5"},"PeriodicalIF":2.3,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40232502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Changes in the circadian rhythm of mRNA expression for µ-opioid receptors in the periaqueductal gray under a neuropathic pain-like state. 神经性疼痛样状态下导水管周围灰质中µ-阿片受体mRNA表达的昼夜节律变化

IF 2.3

Synapse (New York, N.y.) Pub Date : 2013-05-01 Epub Date: 2013-02-08 DOI: 10.1002/syn.21633

Tomohiko Takada, Akira Yamashita, Akitoshi Date, Makoto Yanase, Yuki Suhara, Asami Hamada, Hiroyasu Sakai, Daigo Ikegami, Masako Iseki, Eiichi Inada, Minoru Narita

{"title":"Changes in the circadian rhythm of mRNA expression for µ-opioid receptors in the periaqueductal gray under a neuropathic pain-like state.","authors":"Tomohiko Takada, Akira Yamashita, Akitoshi Date, Makoto Yanase, Yuki Suhara, Asami Hamada, Hiroyasu Sakai, Daigo Ikegami, Masako Iseki, Eiichi Inada, Minoru Narita","doi":"10.1002/syn.21633","DOIUrl":"https://doi.org/10.1002/syn.21633","url":null,"abstract":"Variation in the production of opioid receptors over a 24-h period is considered to contribute to circadian alterations in neuropathic pain. In this study, we investigated the possible changes in the circadian rhythm of mRNA expression for µ-opioid receptor (MOR), κ-opioid receptor (KOR), and adrenaline α2a receptor (α2a) in the periaqueductal gray, frontal cortex, thalamus, and spinal cord following sciatic nerve ligation in mice. In sham-operated mice, the latencies of hind paw-withdrawal in response to thermal stimuli at 14:00 and 20:00 were significantly greater than that at 8:00 and the latency at 2:00 was significantly less than those at 14:00 and 20:00, indicating a \"rest\" period-dominant circadian rhythm for thermal pain-thresholds. In sciatic nerve-ligated mice, the latencies of hind paw-withdrawal in response to thermal stimuli at 14:00 and 20:00 were significantly less than that at 8:00, and the latency at 2:00 was significantly greater than those at 14:00 and 20:00. A correlative tendency between the time-variation of pain latency and the time-variation of MOR mRNA expression was observed in the periaqueductal gray of sham-operated and sciatic nerve-ligated mice. In contrast, neither mouse showed a strong circadian rhythm for the expressions of KOR and α2a mRNAs in any region. The present data suggest that changes in MOR mRNA expression in the periaqueductal gray may be synchronized with the circadian rhythm for the pain threshold for noxious thermal stimuli. In contrast, neuropathic pain in mice exhibited a negative circadian pattern for the expression of MOR, KOR, and α2a receptors in the frontal cortex, thalamus, and spinal cord.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"216-23"},"PeriodicalIF":2.3,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40202393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Chronic methamphetamine exposure produces a delayed, long-lasting memory deficit. 长期接触甲基苯丙胺会导致延迟的、持久的记忆缺陷。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2013-05-01 Epub Date: 2013-02-08 DOI: 10.1002/syn.21635

Ashley North, Jarod Swant, Michael F Salvatore, Joyonna Gamble-George, Petra Prins, Brittany Butler, Mukul K Mittal, Rebecca Heltsley, John T Clark, Habibeh Khoshbouei

{"title":"Chronic methamphetamine exposure produces a delayed, long-lasting memory deficit.","authors":"Ashley North, Jarod Swant, Michael F Salvatore, Joyonna Gamble-George, Petra Prins, Brittany Butler, Mukul K Mittal, Rebecca Heltsley, John T Clark, Habibeh Khoshbouei","doi":"10.1002/syn.21635","DOIUrl":"https://doi.org/10.1002/syn.21635","url":null,"abstract":"Methamphetamine (METH) is a highly addictive and neurotoxic psychostimulant. Its use in humans is often associated with neurocognitive impairment. Whether this is due to long-term deficits in short-term memory and/or hippocampal plasticity remains unclear. Recently, we reported that METH increases baseline synaptic transmission and reduces LTP in an ex vivo preparation of the hippocampal CA1 region from young mice. In the current study, we tested the hypothesis that a repeated neurotoxic regimen of METH exposure in adolescent mice decreases hippocampal synaptic plasticity and produces a deficit in short-term memory. Contrary to our prediction, there was no change in the hippocampal plasticity or short-term memory when measured after 14 days of METH exposure. However, we found that at 7, 14, and 21 days of drug abstinence, METH-exposed mice exhibited a deficit in spatial memory, which was accompanied by a decrease in hippocampal plasticity. Our results support the interpretation that the deleterious cognitive consequences of neurotoxic levels of METH exposure may manifest and persist after drug abstinence. Therefore, therapeutic strategies should consider short-term as well as long-term consequences of methamphetamine exposure.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"245-57"},"PeriodicalIF":2.3,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21635","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40204595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 70

Post-treatment of dextromethorphan on methamphetamine-induced drug-seeking and behavioral sensitization in rats. 右美沙芬对大鼠甲基苯丙胺致药物寻找和行为致敏的后处理。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2012-10-01 Epub Date: 2012-06-26 DOI: 10.1002/syn.21576

Pao-Pao Yang, Eagle Yi-Kung Huang, Yu-Ying Fu, Tsai-Shan Ho, Pao-Luh Tao

{"title":"Post-treatment of dextromethorphan on methamphetamine-induced drug-seeking and behavioral sensitization in rats.","authors":"Pao-Pao Yang, Eagle Yi-Kung Huang, Yu-Ying Fu, Tsai-Shan Ho, Pao-Luh Tao","doi":"10.1002/syn.21576","DOIUrl":"https://doi.org/10.1002/syn.21576","url":null,"abstract":"In our previous study, we first demonstrated a significant effect of dextromethorphan (DM) on morphine-seeking behavior in morphine-dependent rats, when DM was given during morphine withdrawal. Using the same conditioned place preference (CPP) paradigm modified for measuring drug-seeking-related behavior, we further investigated the possible effect of DM on methamphetamine (MA)-seeking in MA-dependent rats. Our data showed that DM could also effectively suppress the drug-seeking behavior for MA, when administered during MA withdrawal. This suggests that DM may possess a pharmacological property to prevent drug-seeking behavior for addictive drugs in general. To examine the action sites of DM in the brain, DM was microinjected into the VTA or the NAc, and tested for its effect on MA-seeking during withdrawal. Both intra-VTA and intra-NAc injections of DM were able to block the MA-seeking, suggesting that DM has a dual action sites. In our neurochemical results, intra-NAc injection of DM showed a clear reduction of DA turnover rate at the NAc and the mPFC in response to MA challenge during withdrawal, which matched with the behavioral results. However, intra-VTA injection of DM reduced the DA turnover rate at the mPFC but did not have effect on the DA turnover rate at the NAc. Although further investigations may be needed to verify the connection between our neurochemical and behavioral results, the present study highlights the therapeutic potential of DM in antidrug-seeking behavior of MA and that the mechanism could be related to its effect on the mesolimbic and mesocortical dopaminergic pathways.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"858-69"},"PeriodicalIF":2.3,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30671857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Hydrocodone and morphine possess similar rewarding effects and reduce ERK and CREB phosphorylation in the nucleus accumbens. 氢可酮和吗啡具有相似的奖赏作用，可降低伏隔核ERK和CREB磷酸化。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2012-10-01 Epub Date: 2012-07-02 DOI: 10.1002/syn.21577

John M Tenayuca, Arbi Nazarian

{"title":"Hydrocodone and morphine possess similar rewarding effects and reduce ERK and CREB phosphorylation in the nucleus accumbens.","authors":"John M Tenayuca, Arbi Nazarian","doi":"10.1002/syn.21577","DOIUrl":"https://doi.org/10.1002/syn.21577","url":null,"abstract":"The number of prescriptions for hydrocodone-containing opioid analgesics has greatly increased over the past decade. This increase has led to an associated enhancement in the nonmedical use of hydrocodone products. There is a lack of evidence to determine the extent of the rewarding effects and signal transduction properties of hydrocodone. Therefore, this study aimed to examine the rewarding properties of hydrocodone (1 and 5 mg/kg) and morphine (1 and 5 mg/kg) using the conditioned place preference paradigm (CPP) in rats. Both hydrocodone and morphine produced a CPP at the 5 mg/kg dose, but not the lower 1 mg/kg dose, suggesting that both drugs possess similar rewarding properties in the CPP paradigm. Moreover, hydrocodone and morphine equally reduced phosphorylation levels of ERK and CREB proteins in the nucleus accumbens, suggesting that both drugs exert their effects through signal transduction pathways known to be involved in drug reward and reinforcement. These findings suggest that hydrocodone should be viewed as similarly capable of producing rewarding and euphoric properties as morphine.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"918-22"},"PeriodicalIF":2.3,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30704216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Subtle biobehavioral effects produced by paternal cocaine exposure. 父亲接触可卡因会产生微妙的生物行为影响。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2012-10-01 Epub Date: 2012-07-27 DOI: 10.1002/syn.21582

Catherine E Killinger, Stacey Robinson, Gregg D Stanwood

引用次数: 41

Gonadectomy differentially regulates steroid receptor coactivator-1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice. 性腺切除术对成年雌性和雄性C57BL/6小鼠海马皮质激素受体共激活因子-1和突触蛋白的调节存在差异。

IF 2.3

Synapse (New York, N.y.) Pub Date : 2012-10-01 Epub Date: 2012-06-13 DOI: 10.1002/syn.21574

Chen Bian, Kongjiang Zhu, Li Yang, Sen Lin, Shurong Li, Bingyin Su, Jiqiang Zhang

{"title":"Gonadectomy differentially regulates steroid receptor coactivator-1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice.","authors":"Chen Bian, Kongjiang Zhu, Li Yang, Sen Lin, Shurong Li, Bingyin Su, Jiqiang Zhang","doi":"10.1002/syn.21574","DOIUrl":"https://doi.org/10.1002/syn.21574","url":null,"abstract":"Hippocampus is one of the most important structures that mediates learning and memory, cognition, and mental behaviors and profoundly regulated by sex hormones in a sex-specific manner, but the mechanism of underlying sex differences regulation is still unclear. We have previously reported that in the male and female mice, steroid receptor coactivator-1 (SRC-1) and some key synaptic proteins share similar developmental profile in the hippocampus, but how circulating sex hormones affect hippocampal SRC-1 as well as these synaptic proteins remain unclear. In this study, we examined how gonad sex hormones regulate hippocampal SRC-1, synaptophysin, PSD-95, and AMPA receptor subtype GluR1 by using immunohistochemistry and Western blot. The results showed that in the female mice, ovariectomy affected hippocampal SRC-1 and GluR1 were only detected at 2 weeks post operation, then it recovered to sham level; synaptophysin was unaffected at any timepoint examined; significant decrease of PSD-95 was only detected at 4 weeks post operation. However, in the male hippocampus, SRC-1 and PSD-95 were decreased from one week and lasted to 4 weeks after orchidectomy, GluR1 decreased from 2 weeks after orchidectomy, but synaptophysin remained unchanged as in the females. Correlation analysis showed the profiles of SRC-1 were positively correlated with GluR1 of the females, PSD-95 and GluR1 of the males, respectively. The above results suggested a distinct regulatory mode between female and male gonad hormones in the regulation of hippocampal SRC-1 and synaptic proteins, which may be one of the mechanisms contributing to the dimorphism of hippocampus during development and ageing.","PeriodicalId":118978,"journal":{"name":"Synapse (New York, N.y.)","volume":" ","pages":"849-57"},"PeriodicalIF":2.3,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/syn.21574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30639699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20