Enzyme Research最新文献_第9页

Stability of a Lipase Extracted from Seeds of Pachira aquatica in Commercial Detergents and Application Tests in Poultry Wastewater Pretreatment and Fat Particle Hydrolysis. 一种从水栖木种子中提取的脂肪酶在商业洗涤剂中的稳定性及其在家禽废水预处理和脂肪颗粒水解中的应用试验。

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-12-23 DOI: 10.1155/2013/324061

Patrícia Peres Polizelli, Fernanda Dell Antonio Facchini, Gustavo Orlando Bonilla-Rodriguez

引用次数: 12

Immobilization of α-Amylase onto Luffa operculata Fibers. α-淀粉酶在丝瓜纤维上的固定化。

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-03-31 DOI: 10.1155/2013/803415

Ricardo R Morais, Aline M Pascoal, Samantha S Caramori, Flavio M Lopes, Kátia F Fernandes

引用次数: 9

Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol. 在含山梨醇改性纤维素的浸没培养基中青霉生产纤维素酶和木聚糖酶。

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-08-22 DOI: 10.1155/2013/240219

Carla Eliana Todero Ritter, Marli Camassola, Denise Zampieri, Mauricio Moura Silveira, Aldo José Pinheiro Dillon

{"title":"Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol.","authors":"Carla Eliana Todero Ritter, Marli Camassola, Denise Zampieri, Mauricio Moura Silveira, Aldo José Pinheiro Dillon","doi":"10.1155/2013/240219","DOIUrl":"https://doi.org/10.1155/2013/240219","url":null,"abstract":"The present work investigated the use of sorbitol as a soluble carbon source, in association with cellulose, to produce cellulases and xylanases in submerged cultures of Penicillium echinulatum 9A02S1. Because cellulose is an insoluble carbon source, in cellulase production, there are some problems with rheology and oxygen transfer. The submerged fermentations containing media composed of 0, 0.25, 0.5, 0.75, and 1% (w/v) sorbitol and cellulose that were added at different times during the cultivation; 0.2% (w/v) soy bran; 0.1% (w/v) wheat bran; and a solution of salts. The highest filter paper activity (FPA) (1.95 ± 0.04 IU·mL(-1)) was obtained on the seventh day in the medium containing 0.5% (w/v) sorbitol and 0.5% (w/v) cellulose added 24 h after the start of cultivation. However, the CMCases showed an activity peak on the sixth day (9.99 ± 0.75 IU·mL(-1)) in the medium containing 0.75% (w/v) sorbitol and 0.75% (w/v) cellulose added after 12 h of cultivation. The xylanases showed the highest activity in the medium with 0.75% (w/v) sorbitol and 0.25% (w/v) cellulose added 36 h after the start of cultivation. This strategy enables the reduction of the cellulose concentration, which in high concentrations can cause rheological and oxygen transfer problems. ","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/240219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31752409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

A Highly Thermostable Xylanase from Stenotrophomonas maltophilia: Purification and Partial Characterization. 嗜麦芽寡养单胞菌的高耐热木聚糖酶:纯化和部分特性。

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-12-14 DOI: 10.1155/2013/429305

Abhay Raj, Sharad Kumar, Sudheer Kumar Singh

{"title":"A Highly Thermostable Xylanase from Stenotrophomonas maltophilia: Purification and Partial Characterization.","authors":"Abhay Raj, Sharad Kumar, Sudheer Kumar Singh","doi":"10.1155/2013/429305","DOIUrl":"https://doi.org/10.1155/2013/429305","url":null,"abstract":"Seven xylanolytic bacterial strains were isolated from saw-dust dump soil. The bacterial strain X6 was selected on the basis of the highest xylanase activity with no cellulase contamination. It was identified as Stenotrophomonas maltophilia by biochemical tests and 16S rRNA gene sequencing approach. Xylanase production studies by S. maltophilia on different commercial xylans and agro-industrial residues suggested that wheat bran was the best carbon source for xylanase production (26.4 ± 0.6 IU/mL). The studies with inorganic and organic nitrogen sources suggested yeast extract as the best support for xylanase production (25 ± 0.6 IU/mL). Maximum xylanase production was observed at initial medium pH = 8.0 (23.8 ± 0.4 IU/mL) with production at pH = 7.0 and pH = 9.0 being almost comparable. Xylanase produced by S. maltophilia was purified to homogeneity using ammonium sulfate precipitation, gel filtration, and ion exchange chromatography. The final purification was 5.43-fold with recovery of 19.18%. The molecular weight of the purified xylanase protein was ~142 kDa. Both crude and purified xylanase had good stability at pH = 9.0 and 80°C with activity retention greater than 90% after 30 min incubation. The enzyme stability at high temperature and alkaline pH make it potentially effective for industrial applications. ","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/429305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32023640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation. 嗜热真菌金热曲霉CBMAI-756固态发酵产聚半乳糖醛酸酶的纯化及性能研究

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-09-12 DOI: 10.1155/2013/438645

Eduardo da Silva Martins, Rodrigo Simões Ribeiro Leite, Roberto da Silva, Eleni Gomes

{"title":"Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation.","authors":"Eduardo da Silva Martins, Rodrigo Simões Ribeiro Leite, Roberto da Silva, Eleni Gomes","doi":"10.1155/2013/438645","DOIUrl":"https://doi.org/10.1155/2013/438645","url":null,"abstract":"Polygalacturonases are enzymes involved in the degradation of pectic substances, being extensively used in food industries, textile processing, degumming of plant rough fibres, and treatment of pectic wastewaters. Polygalacturonase (PG) production by thermophilic fungus Thermoascus aurantiacus on solid-state fermentation was carried out in culture media containing sugar cane bagasse and orange bagasse in proportions of 30% and 70% (w/w) at 45°C for 4 days. PG obtained was purified by gel filtration and ion-exchange chromatography. The highest activity was found between pH 4.5 and 5.5, and the enzyme preserved more than 80% of its activity at pH values between 5.0 and 6.5. At pH values between 3.0 and 4.5, PG retained about 73% of the original activity, whereas at pH 10.0 it remained around 44%. The optimum temperature was 60-65°C. The enzyme was completely stable when incubated for 1 hour at 50°C. At 55°C and 60°C, the activity decreased 55% and 90%, respectively. The apparent molecular weight was 29.3 kDa, K m of 1.58 mg/mL and V max of 1553.1 μ mol/min/mg. The presence of Zn(+2), Mn(+2), and Hg(+2) inhibited 59%, 77%, and 100% of enzyme activity, respectively. The hydrolysis product suggests that polygalacturonase was shown to be an endo/exoenzyme. ","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/438645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40265794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. 鲍曼不动杆菌核苷二磷酸激酶的克隆、表达和纯化。

Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-04-11 DOI: 10.1155/2013/597028

Juhi Sikarwar, Sanket Kaushik, Mau Sinha, Punit Kaur, Sujata Sharma, Tej P Singh

引用次数: 0

In silico characterization of histidine Acid phytase sequences. 组氨酸植酸酶序列的硅学特征。

Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-12-05 DOI: 10.1155/2012/845465

Vinod Kumar, Gopal Singh, A K Verma, Sanjeev Agrawal

{"title":"In silico characterization of histidine Acid phytase sequences.","authors":"Vinod Kumar, Gopal Singh, A K Verma, Sanjeev Agrawal","doi":"10.1155/2012/845465","DOIUrl":"10.1155/2012/845465","url":null,"abstract":"Histidine acid phytases (HAPhy) are widely distributed enzymes among bacteria, fungi, plants, and some animal tissues. They have a significant role as an animal feed enzyme and in the solubilization of insoluble phosphates and minerals present in the form of phytic acid complex. A set of 50 reference protein sequences representing HAPhy were retrieved from NCBI protein database and characterized for various biochemical properties, multiple sequence alignment (MSA), homology search, phylogenetic analysis, motifs, and superfamily search. MSA using MEGA5 revealed the presence of conserved sequences at N-terminal \"RHGXRXP\" and C-terminal \"HD.\" Phylogenetic tree analysis indicates the presence of three clusters representing different HAPhy, that is, PhyA, PhyB, and AppA. Analysis of 10 commonly distributed motifs in the sequences indicates the presence of signature sequence for each class. Motif 1 \"SPFCDLFTHEEWIQYDYLQSLGKYYGYGAGNPLGPAQGIGF\" was present in 38 protein sequences representing clusters 1 (PhyA) and 2 (PhyB). Cluster 3 (AppA) contains motif 9 \"KKGCPQSGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDP\" as a signature sequence. All sequences belong to histidine acid phosphatase family as resulted from superfamily search. No conserved sequence representing 3- or 6-phytase could be identified using multiple sequence alignment. This in silico analysis might contribute in the classification and future genetic engineering of this most diverse class of phytase.","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31147492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation of enzyme catalysis in calcium alginate beads. 海藻酸钙微球酶催化的模拟。

Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-10-31 DOI: 10.1155/2012/459190

Ameel M R Al-Mayah

{"title":"Simulation of enzyme catalysis in calcium alginate beads.","authors":"Ameel M R Al-Mayah","doi":"10.1155/2012/459190","DOIUrl":"https://doi.org/10.1155/2012/459190","url":null,"abstract":"A general mathematical model for a fixed bed immobilized enzyme reactor was developed to simulate the process of diffusion and reaction inside the biocatalyst particle. The modeling and simulation of starch hydrolysis using immobilized α-amylase were used as a model for this study. Corn starch hydrolysis was carried out at a constant pH of 5.5 and temperature of 50°C. The substrate flow rate was ranging from 0.2 to 5.0 mL/min, substrate initial concentrations 1 to 100 g/L. α-amylase was immobilized on to calcium alginate hydrogel beads of 2 mm average diameter. In this work Michaelis-Menten kinetics have been considered. The effect of substrate flow rate (i.e., residence time) and initial concentration on intraparticle diffusion have been taken into consideration. The performance of the system is found to be affected by the substrate flow rate and initial concentrations. The reaction is controlled by the reaction rate. The model equation was a nonlinear second order differential equation simulated based on the experimental data for steady state condition. The simulation was achieved numerically using FINITE ELEMENTS in MATLAB software package. The simulated results give satisfactory results for substrate and product concentration profiles within the biocatalyst bead.","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/459190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31084275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Isolation and characterization of chitosan-producing bacteria from beaches of chennai, India. 印度金奈海滩产壳聚糖细菌的分离与鉴定。

Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-08-05 DOI: 10.1155/2012/421683

Kuldeep Kaur, Vikrant Dattajirao, Vikas Shrivastava, Uma Bhardwaj

引用次数: 46

Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury. 用荧光标记的半胱氨酸酶抑制剂检测脊髓损伤后全载七鳃鳗脑内神经元凋亡。

Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-07-08 DOI: 10.1155/2012/835731

Antón Barreiro-Iglesias, Michael I Shifman

{"title":"Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.","authors":"Antón Barreiro-Iglesias, Michael I Shifman","doi":"10.1155/2012/835731","DOIUrl":"https://doi.org/10.1155/2012/835731","url":null,"abstract":"Apoptosis is a major feature in neural development and important in traumatic diseases. The presence of active caspases is a widely accepted marker of apoptosis. We report here the development of a method to study neuronal apoptotic death in whole-mounted brain preparations using fluorochrome-labeled inhibitors of caspases (FLICA). As a model we used axotomy-induced retrograde neuronal death in the CNS of larval sea lampreys. Once inside the cell, the FLICA reagents bind covalently to active caspases causing apoptotic cells to fluoresce, whereas nonapoptotic cells remain unstained. The fluorescent probe, the poly caspase inhibitor FAM-VAD-FMK, was applied to whole-mounted brain preparations of larval sea lampreys 2 weeks after a complete spinal cord (SC) transection. Specific labeling occurred only in identifiable spinal-projecting neurons of the brainstem previously shown to undergo apoptotic neuronal death at later times after SC transection. These neurons also exhibited intense labeling 2 weeks after a complete SC transection when a specific caspase-8 inhibitor (FAM-LETD-FMK) served as the probe. In this study we show that FLICA reagents can be used to detect specific activated caspases in identified neurons of the whole-mounted lamprey brain. Our results suggest that axotomy may cause neuronal apoptosis by activation of the extrinsic apoptotic pathway.","PeriodicalId":11835,"journal":{"name":"Enzyme Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/835731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30788305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33