Environmental and Molecular Mutagenesis最新文献_第9页

Unbiased whole genome detection of ultrarare off-target mutations in genome-edited cell populations by HiFi sequencing 通过HiFi测序无偏全基因组检测基因组编辑细胞群体中的超罕见脱靶突变

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-24 DOI: 10.1002/em.22566

Jaime A. Miranda, Kristina Fenner, Page B. McKinzie, Vasily N. Dobrovolsky, Javier R. Revollo

{"title":"Unbiased whole genome detection of ultrarare off-target mutations in genome-edited cell populations by HiFi sequencing","authors":"Jaime A. Miranda, Kristina Fenner, Page B. McKinzie, Vasily N. Dobrovolsky, Javier R. Revollo","doi":"10.1002/em.22566","DOIUrl":"10.1002/em.22566","url":null,"abstract":"DNA base editors (BEs) composed of a nuclease-deficient Cas9 fused to a DNA-modifying enzyme can achieve on-target mutagenesis without creating double-strand DNA breaks (DSBs). As a result, BEs generate far less DNA damage than traditional nuclease-proficient Cas9 systems, which do rely on the creation of DSBs to achieve on-target mutagenesis. The inability of BEs to create DSBs makes the detection of their undesired off-target effects very difficult. PacBio HiFi sequencing can efficiently detect ultrarare mutations resulting from chemical mutagenesis in whole genomes with a sensitivity ~1 × 10−8 mutations per base pair. In this proof-of-principle study, we evaluated whether this technique could also detect the on- and off-target mutations generated by a cytosine-to-thymine (C>T) BE targeting the LacZ gene in Escherichia coli (E. coli). HiFi sequencing detected on-target mutant allele fractions ranging from ~7% to ~63%, depending on the single-guide RNA (sgRNA) used, while no on-target mutations were detected in controls lacking the BE. The presence of the BE resulted in a ~3-fold increase in mutation frequencies compared to controls lacking the BE, irrespective of the sgRNA used. These increases were mostly composed of C:G>T:A substitutions distributed throughout the genome. Our results demonstrate that HiFi sequencing can efficiently identify on- and off-target mutations in cell populations that have undergone genome editing.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 7","pages":"374-381"},"PeriodicalIF":2.8,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10092557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium exposure induces necroptosis of porcine spleen via ROS-mediated activation of STAT1/RIPK3 signaling pathway 镉暴露通过ros介导的STAT1/RIPK3信号通路激活诱导猪脾脏坏死

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-15 DOI: 10.1002/em.22565

Yu Xia, Yiming Zhang, Jintao Zhang, Yongzhen Du, Yixuan Wang, Anqi Xu, Shu Li

{"title":"Cadmium exposure induces necroptosis of porcine spleen via ROS-mediated activation of STAT1/RIPK3 signaling pathway","authors":"Yu Xia, Yiming Zhang, Jintao Zhang, Yongzhen Du, Yixuan Wang, Anqi Xu, Shu Li","doi":"10.1002/em.22565","DOIUrl":"10.1002/em.22565","url":null,"abstract":"Cadmium (Cd), a heavy metal, is used in a wide range of applications, such as plastics, electroplating process, electronics, and so forth. Due to its bioaccumulation ability, Cd can contaminate soil, water, air and food. To determine the effect of Cd exposure on the necroptosis in pig spleen and its mechanistic investigation, we constructed a model in pigs by feeding them food containing 20 mg/kg Cd. In this study, we analyzed the effects of Cd exposure on pig spleen through HE staining, Quantitative real-time PCR (qRT-PCR), Western blot (WB), and principal component analysis (PCA). Results show that Cd exposure can destroy the structure and function of pig spleen, which is closely related to necroptosis. Further results show that Cd exposure can induce necroptosis through ROS-mediated activation of Signal transducer and activator of transcription 1/Receptor-Interacting Serine/Threonine-Protein Kinase 3 (STAT1/RIPK3) signaling pathway in pig spleen. Additionally, Cd exposure also can affect the stability of mitochondrial-associated endoplasmic reticulum membrane (MAMs) structure, which also contributes to the process of necroptosis. Our study provides insights into the physiological toxicity caused by Cd exposure.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 7","pages":"382-392"},"PeriodicalIF":2.8,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10214519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Templated insertions—DNA repair gets acrobatic 模板化插入--DNA 修复变得杂技化

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-12 DOI: 10.1002/em.22564

Susanna Stroik, Adam J. Luthman, Dale A. Ramsden

{"title":"Templated insertions—DNA repair gets acrobatic","authors":"Susanna Stroik, Adam J. Luthman, Dale A. Ramsden","doi":"10.1002/em.22564","DOIUrl":"10.1002/em.22564","url":null,"abstract":"Deletions associated with the repair of DNA double-strand breaks is a source of genetic alternation and a recognized source of disease-causing mutagenesis. Theta-mediated end joining is a DNA repair mechanism, which guarantees deletions by its employment of microhomology (MH) alignment to facilitate end joining. A lesser-characterized templated insertion ability of this pathway, on the other hand, is associated with both deletion and insertion. This mechanism is characterized by at least one round of polymerase θ-mediated synthesis, which does not result in successful repair, followed by a subsequent round of polymerase engagement and synthesis that does lead to repair. Here we focus on the mechanisms by which polymerase θ introduces these insertions—direct, inverse, and a new class which we have termed strand switching. We observe this new class of templated insertions at multiple loci and across multiple species, often at a comparable frequency to those previously characterized. Templated insertion mutations are often enriched in cancer genomes and repeat expansion disorders. This repair mechanism thus contributes to disease-associated mutagenesis, and may plausibly even promote disease. Characterization of the types of polymerase θ-dependent insertions can provide new insight into these diseases and clinical promise for treatment.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"65 S1","pages":"82-89"},"PeriodicalIF":2.8,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10257790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetics of the in vivo genotoxic and radioprotective effects of methyl gallate and epigallocatechin gallate 没食子儿茶素没食子酸甲酯和没食子儿茶素没食子酸甲酯的体内遗传毒性和放射防护作用动力学

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-10 DOI: 10.1002/em.22563

Virginia Cruz-Vallejo, Anaís Zarco-Mendoza, Pedro Morales-Ramírez

{"title":"Kinetics of the in vivo genotoxic and radioprotective effects of methyl gallate and epigallocatechin gallate","authors":"Virginia Cruz-Vallejo, Anaís Zarco-Mendoza, Pedro Morales-Ramírez","doi":"10.1002/em.22563","DOIUrl":"10.1002/em.22563","url":null,"abstract":"The aim of this study was to compare the kinetics of the in vivo action of equimolar doses of methyl gallate (MG) and epigallocatechin gallate (EGCG) on their capacity to induce DNA damage and to protect against DNA damage induced by 60Co gamma rays. DNA-damaged cells were determined by single-cell gel electrophoresis (comets) in murine peripheral blood leukocytes. The maximum radioprotective effects of MG and EGCG (approximately 70%) occurred at 15 min after administration when their effect was determined 2 min following irradiation. MG and EGCG have similar radioprotective indexes, which due to their fast response indicate that they are involved in free radical scavenging. Due to the similar radioprotective activities of MG and EGCG, the in vivo radioprotective effects of these agents do not seem to be dependent on the number of hydroxyl groups present in their structures but instead on the presence of the galloyl radical. EGCG induces an early, significant, and persistent increase in the number of DNA-damaged cells and a later and more important increase in the number of damaged cells, suggesting that it has two mechanisms by which it can induce DNA damage. MG at the same molar dose as EGCG caused a significant and persistent increase in DNA damaged cells but to a much lesser extent to that induce by EGCG, suggesting that the galloyl radical is not involved in the mechanism of DNA breaks induction.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 7","pages":"393-400"},"PeriodicalIF":2.8,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10101950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome damage in regions with different levels of air pollution 不同空气污染程度地区的染色体损伤

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-08 DOI: 10.1002/em.22562

Petra Musilova, Dita Kadlcikova, Hana Hradska, Miluse Vozdova, Iveta Selingerova, Halina Cernohorska, Dusan Salat, Jiri Rubes

{"title":"Chromosome damage in regions with different levels of air pollution","authors":"Petra Musilova, Dita Kadlcikova, Hana Hradska, Miluse Vozdova, Iveta Selingerova, Halina Cernohorska, Dusan Salat, Jiri Rubes","doi":"10.1002/em.22562","DOIUrl":"10.1002/em.22562","url":null,"abstract":"Air pollution is an important environmental factor influencing human health. In this study, we compared chromosome damage in city policemen from three cities in the Czech Republic: industrial Ostrava characterized by high levels of benzo[a]pyrene, Prague with heavy traffic emitting nitrogen oxides, and relatively clean Ceske Budejovice located in an area with predominantly agricultural activity. Chromosomal aberrations in lymphocytes were evaluated by fluorescence in situ hybridization with painting probes for chromosomes 1, 2, 3, and 4 in spring and autumn. An increase in the frequency of unstable chromosome aberrations, that is, dicentric chromosomes and acentric fragments, was observed in spring samples from Ostrava (p = .014 and p = .044, respectively) and Prague (p = .002 and p = .006, respectively) in comparison with Ceske Budejovice. The difference was significant only for samples taken after the winter period, when the concentration of pollutants in the air increases due to poor dispersion conditions. An increased frequency of dicentric chromosomes was observed in spring compared to autumn in both Ostrava and Prague (p = .017 and p = .023, respectively), but not in Ceske Budejovice. More breakpoints were observed on chromosome 1 than on the other chromosomes examined (p < .001). The number of breakpoints in the heterochromatin region 1p11-q12 was lower than in other parts of chromosome 1 (p < .001), suggesting a protective function of heterochromatin against damage. Our study showed, that air pollution increased the frequency of unstable chromosome aberrations, especially dicentric chromosomes. However, we did not show an effect on stable chromosome rearrangements.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 6","pages":"326-334"},"PeriodicalIF":2.8,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/em.22562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9884020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity and mutagenicity studies of 6PPD-quinone in a marine invertebrate species and bacteria 6ppd -醌对海洋无脊椎动物和细菌的毒性和致突变性研究

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-04 DOI: 10.1002/em.22560

Marina Tenório Botelho, Gabriely Groto Militão, Markus Brinkmann, Gisela de Aragão Umbuzeiro

{"title":"Toxicity and mutagenicity studies of 6PPD-quinone in a marine invertebrate species and bacteria","authors":"Marina Tenório Botelho, Gabriely Groto Militão, Markus Brinkmann, Gisela de Aragão Umbuzeiro","doi":"10.1002/em.22560","DOIUrl":"10.1002/em.22560","url":null,"abstract":"N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6PPD-quinone), an oxidation product of the tire additive, 6PPD, has been associated with high mortality of salmonids (0.1 μg/L). The objective of this study was to determine the acute toxicity using neonates and mutagenicity (micronuclei in hemolymph of exposed adults) of 6PPD-quinone in the marine amphipod Parhyale hawaiensis. Also, we studied its mutagenicity in the Salmonella/microsome assay using five strains of Salmonella with and without metabolic system (rat liver S9, 5%). 6PPD-quinone did not present acute toxicity to P. hawaiensis from 31.25 to 500 μg/L. Micronuclei frequency increased after 96 h-exposure to 6PPD-quinone (250 and 500 μg/L) when compared to the negative control. 6PPD-quinone also showed a weak mutagenic effect for TA100 only in the presence of S9. We conclude that 6PPD-quinone is mutagenic to P. hawaiensis and weakly mutagenic to bacteria. Our work provides information for future risk assessment of the presence of 6PPD-quinone in the aquatic environment.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 6","pages":"335-341"},"PeriodicalIF":2.8,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Issue Information 问题信息

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-01 DOI: 10.1002/wer.10736

引用次数: 0

Downregulation of circular RNA hsa_circ_0087856 sensitizes bladder cancer cells to cisplatin through targeting miR-1184/CITED2 signaling 下调环状RNA hsa_circ_0087856通过靶向miR-1184/CITED2信号通路使膀胱癌细胞对顺铂敏感

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-07-01 DOI: 10.1002/em.22561

Min Ju, Weiwei Wu, Jingkun Qu, Yang Sun, Jun Li

{"title":"Downregulation of circular RNA hsa_circ_0087856 sensitizes bladder cancer cells to cisplatin through targeting miR-1184/CITED2 signaling","authors":"Min Ju, Weiwei Wu, Jingkun Qu, Yang Sun, Jun Li","doi":"10.1002/em.22561","DOIUrl":"10.1002/em.22561","url":null,"abstract":"CircRNAs are considered as one of the potential therapeutic targets of multiple cancers. According to accumulating evidence, circRNA regulates cancer progression by acting as a miRNA sponge. In the current work, our data discovered that hsa_circ_0087856 and CITED2 expression was increased, while miR-1184 expression was decreased in BC cell lines and tissues. Hsa_circ_0087856 expression negatively correlated with miR-1184, whereas positively correlated with CITED2. Hsa_circ_0087856 silencing suppressed BC tumor growth, and contributed to the inhibition of cisplatin to tumor growth. In cellular experiments, hsa_circ_0087856 increasing promoted BC cells proliferation, migration and invasion, and inhibited the cells apoptosis. Hsa_circ_0087856 increasing partly reversed the inhibition of cisplatin to BC cell proliferation and the promotion to cell apoptosis. Oppositely, hsa_circ_0087856 silencing could increase the sensitivity of BC cells to cisplatin. Hsa_circ_0087856 promoted CITED2 expression through binding with miR-1184 and inhibiting its expression. CITED2 increasing partly reversed the promotion of hsa_circ_0087856 silencing to cisplatin-induced BC cells apoptosis promotion and proliferation suppression. Overall, our results revealed the role of hsa_circ_0087856 that downregulation its expression could enhance the BC cells sensitivity to cisplatin by facilitating CITED expression via sponging miR-1184. Moreover, our research provided a potential therapeutic target for BC.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 6","pages":"342-353"},"PeriodicalIF":2.8,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9883532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0000033 up-regulates NUAK2 by sequestering miR-378a-3p to promote breast tumorigenesis Circ_0000033通过隔离miR-378a-3p上调NUAK2，促进乳腺肿瘤发生

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-06-25 DOI: 10.1002/em.22558

Yijun Dai, Wenjian Shi, Yanru Qiu, Tianwen Xu, Jianguang Lin, Yunxia Su

{"title":"Circ_0000033 up-regulates NUAK2 by sequestering miR-378a-3p to promote breast tumorigenesis","authors":"Yijun Dai, Wenjian Shi, Yanru Qiu, Tianwen Xu, Jianguang Lin, Yunxia Su","doi":"10.1002/em.22558","DOIUrl":"10.1002/em.22558","url":null,"abstract":"Circular RNAs (circRNAs), including circ_0000033, were shown to be abnormally expressed in breast cancer (BC) and play an important regulatory function in the development of this cancer. This study aimed to investigate the action and mechanism of circ_0000033 in BC carcinogenesis. Specifically, levels of genes and proteins were analyzed using quantitative real-time PCR (qRT-PCR) and western blotting. Circ_0000033 was highly expressed in BC tissues and cells. Properties of cells with modified expression of circ_0000033 were characterized using an in vitro colony formation assay, EdU assay, flow cytometry, caspase-3 activity analysis, transwell assay, and tube formation assay, respectively. Functionally, knockdown of circ_0000033 suppressed BC cell proliferation, migration, invasion, angiogenesis, and induced apoptosis and cell cycle arrest in vitro. An in vivo experiment was conducted using a murine xenograft model and showed circ_0000033 silencing also impeded the growth of BC in nude mice. The binding between miR-378a-3p and circ_0000033 or NUAK2 (NUAK Family Kinase 2) was validated using a dual-luciferase reporter assay. Circ_0000033 sequestered miR-378a-3p and resulted in NUAK2 release, indicating a circ_0000033/miR-378a-3p/NUAK2 regulatory network operates in BC cells. Circ_0000033 down-regulation in BC cells was accompanied by decreased NUAK2 and increased miR-378a-3p expression. Moreover, the anticancer effects mediated by circ_0000033 knockdown were abolished by miR-378a-3p inhibition or NUAK2 overexpression in BC cells. Overall, circ_0000033 up-regulates NUAK2 through sequestration miR-378a-3p, which promoted breast tumorigenesis, suggesting circ_0000033 is a promising therapeutic target for BC treatment.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 6","pages":"359-370"},"PeriodicalIF":2.8,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9883526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations between KCNQ1OT1 genetic variation rs10766212 and susceptibility to colorectal cancer and clinical stage in a Chinese Han population kcnq10t1基因变异rs10766212与中国汉族人群结直肠癌易感性和临床分期的关系

IF 2.8 4区医学

Environmental and Molecular Mutagenesis Pub Date : 2023-06-22 DOI: 10.1002/em.22559

Wanjia Nie, Shulong Zhang, Xueren Gao

{"title":"Associations between KCNQ1OT1 genetic variation rs10766212 and susceptibility to colorectal cancer and clinical stage in a Chinese Han population","authors":"Wanjia Nie, Shulong Zhang, Xueren Gao","doi":"10.1002/em.22559","DOIUrl":"10.1002/em.22559","url":null,"abstract":"KCNQ1OT1 has been linked to the development and progression of colorectal cancer (CRC). As a result, functional polymorphisms in the KCNQ1OT1 gene may have a role in CRC formation and progression. The goal of this study was to see if the rs10766212 polymorphism on the KCNQ1OT1 gene was linked to CRC susceptibility and clinical stage in a Chinese Han population. The case–control research comprised a total of 576 CRC patients and 606 healthy controls. The genotype of the rs10766212 polymorphic locus was determined using the Sanger sequencing technique. We found that the KCNQ1OT1 rs10766212 polymorphism was not related to CRC susceptibility; however, it was connected with the clinical stage of CRC. Patients with CRC who had the rs10766212 T allele had a lower risk of stage III/IV tumors than those who had the rs10766212 C allele. Furthermore, CRC tissues with the rs10766212 CC genotype showed a significant negative connection between KCNQ1OT1 and hsa-miR-622 expression. The luciferase assay showed that the rs10766212 C allele might contribute to the adsorption of KCNQ1OT1 on hsa-miR-622. In conclusion, the rs10766212 polymorphism altering hsa-miR-622 binding is linked to the clinical stage of CRC and may serve as a biomarker for predicting CRC progression in the Chinese Han population. However, better-designed studies are still needed to confirm the current findings.","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"64 6","pages":"354-358"},"PeriodicalIF":2.8,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9957598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0