Environmental Epigenetics最新文献

{"title":"Molecular responses of chicken embryos to maternal heat stress through DNA methylation and gene expression: a pilot study.","authors":"Keyvan Karami, Jules Sabban, Chloé Cerutti, Guillaume Devailly, Sylvain Foissac, David Gourichon, Alexandre Hubert, Jean-Noël Hubert, Sophie Leroux, Tatiana Zerjal, Sandrine Lagarrigue, Frédérique Pitel","doi":"10.1093/eep/dvaf009","DOIUrl":"10.1093/eep/dvaf009","url":null,"abstract":"<p><p>Climate change, with its repercussions on agriculture, is one of the most important adaptation challenges for livestock production. Poultry production is a major source of proteins for human consumption all over the world. With a growing human population, improving poultry's adaptation to environmental constraints becomes critical. Extensive evidence highlights the influence of environmental variations on epigenetic modifications. The aim of this paper is therefore to explore chickens' molecular response to maternal heat stress. We employed Reduced Representation Bisulfite Sequencing to generate genome-wide single-base resolution DNA methylation profiling and RNA sequencing to profile the transcriptome of the brains of embryos hatched from dams reared under either heat stress (32°C) or thermoneutrality (22°C). We detected 289 significant differentially methylated CpG sites (DMCs) and one differentially methylated region (DMR) between heat stressed and control groups. These DMCs were associated with 357 genes involved in processes such as cellular response to stimulus, developmental processes, and immune function. In addition, we identified 11 genes differentially expressed between the two groups of embryos, and identified ATP9A as a target gene of maternal heat stress on offspring. This study provides a body of fundamental knowledge on adaptive mechanisms concerning heat tolerance in chickens.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf009"},"PeriodicalIF":4.8,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144282952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering differences in DNA methylation and transcriptome profiles of oocytes from pigs with high and low developmental competence.","authors":"Laura Abril-Parreño, Jordana S Lopes, Jon Romero-Aguirregomezcorta, Antonio Galvao, Gavin Kelsey, Pilar Coy","doi":"10.1093/eep/dvaf018","DOIUrl":"10.1093/eep/dvaf018","url":null,"abstract":"<p><p><i>In vitro</i> maturation (IVM) is a critical step in animal <i>in vitro</i> embryo production, yet oocytes matured <i>in vitro</i> often exhibit lower developmental competence than their <i>in vivo</i> counterparts. However, the molecular mechanisms behind this observation remain unclear. This study investigated the gene expression and DNA methylation profiles in porcine oocytes with different developmental competencies. To study these differences, we used as a model oocytes from prepubertal gilts (IVM) and sows (<i>in vivo</i> matured) and assessed their developmental competence up to the blastocyst stage. We also examined their gene expression and DNA methylation profiles at single-cell resolution using RNA sequencing and bisulfite sequencing. Oocytes were obtained by aspiration of either ovarian follicles between 3 and 6 mm diameter, and the subsequent IVM, or ovarian follicles from 8 to 10 mm diameter, with no need for maturation (<i>in vivo</i> matured oocytes). Cleavage rates (58.2 ± 3.0 and 45.7 ± 4.4) and blastocyst rates (31.4 ± 3.7 and 47.5 ± 6.6) for IVM and <i>in vivo</i> groups differed significantly. Using the <i>in vivo</i> group as a reference, IVM oocytes had 1297 downregulated and 476 upregulated differentially expressed genes (DEGs), with upregulated DEGs associated with organelle organization and cell cycle processes, and downregulated genes involved in protein synthesis and metabolomic processes. While global DNA methylation levels were similar between groups, a few differentially methylated regions were found in CpG islands, promoters, and coding regions. Our integrative analysis identified key methylated regions and genes that distinguish each group, suggesting that both donor age and maturation conditions significantly influence gene expression regulation in oocytes with different developmental competencies.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf018"},"PeriodicalIF":4.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping heat stress-induced core histone post-translational modifications in <i>Acropora cervicornis</i>.","authors":"Cassandra N Fuller, Sabrina Mansoor, Santiago J Guzman, Lilian Valadares Tose, Serena Hackerott, Javier Rodriguez-Casariego, Jose M Eirin-Lopez, Francisco Fernandez-Lima","doi":"10.1093/eep/dvaf017","DOIUrl":"10.1093/eep/dvaf017","url":null,"abstract":"<p><p>Histone post-translational modifications (PTMs) participate in the dynamic regulation of chromatin structure and function, through their chemical nature and specific location within the histone sequence. Alternative analytical approaches for histone PTM studies are required to facilitate the differentiation between ubiquitously present isomers and the detection of low-abundance PTMs Here, we report a high-sensitivity bottom-up method based on nano-liquid chromatography (nLC), trapped ion mobility spectrometry (TIMS), data-dependent acquisition (DDA), parallel accumulation-serial fragmentation (PASEF), and high-resolution time-of-flight tandem mass spectrometry (ToF-MS/MS) for the analysis of histone PTMs. This method was tested in a threatened coral species, the staghorn coral <i>Acropora cervicornis</i>, during an episode of acute thermal stress. The obtained results allowed for the identification of PTM changes in core histones involved in the coral's heat response. Compared to traditional LC-MS/MS approaches, the incorporation of TIMS and ddaPASEF MS/MS resulted in a highly specific and sensitive method with a wide dynamic range (6 orders of magnitude). This depth of analysis allows for the simultaneous measurement of low-abundance PTM signatures relative to the unmodified form. An added advantage is the ability to mass- and mobility-isolate prior to peptide sequencing, resulting in higher confidence identification of epigenetic markers associated with heat stress in corals (e.g. increased H4 4-17 with 2ac and 3ac, and decreases in H4 4-17 K12ac, K16ac, H4 K20me₂, and H2A K5ac, K7ac, K9ac, K12ac, K14ac, and K74ac).</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf017"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immediate and durable effects of maternal tobacco consumption on placental DNA methylation: a replication and discovery study.","authors":"Chloé Masdoumier, Lucile Broséus, Florent Chuffart, Olivier François, Ariane Guilbert, Barbara Heude, Saadi Khochbin, Sophie Rousseaux, Emie Seyve, Muriel Tafflet, Jorg Tost, Aurélie Nakamura, Johanna Lepeule","doi":"10.1093/eep/dvaf016","DOIUrl":"10.1093/eep/dvaf016","url":null,"abstract":"<p><p>An increasing number of epigenome-wide association studies report tobacco smoking-associated DNA methylation levels. However, comprehensive replication studies remain scarce, particularly in placenta, despite their crucial interest in such a large-scale context. Using DNA methylation data from the EPIC array of 341 new placentas (85 smokers, 219 non-smokers, and 37 former smokers) from the EDEN cohort, we used a candidate approach to replicate maternal smoking-associated CpGs and regions previously identified using the 450K array, and an exploratory approach to discover new associations within EPIC-specific CpGs. Smoking-associated changes in DNA methylation in CpGs and regions were classified as either transient or persistent (indicating epigenetic memory), depending on the stability of their association with smoking status. Among candidate loci, 38% of probes and 9% of regions were replicated, providing robust evidence of effects of prenatal smoke exposure on methylation patterns of these loci. <i>LEKR1</i> was the top hit in both the initial and replication studies. Most of the replicated loci were transient CpGs (i.e. current smokers), while persistent CpGs (i.e. former smokers) remained scarce and somewhat inconsistent with previous findings. The additional exploratory analysis identified 733 novel probes and 75 novel regions, including 18% and 30% of transient loci, respectively. Results suggested that most of the effects were related to <i>in utero</i> exposure only, supporting pregnant women's efforts to quit smoking. This replication study also evidences the importance of reproducible work in omic investigations to provide a more in-depth and robust understanding of the effects of environmental exposures on health biomarkers..</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf016"},"PeriodicalIF":4.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal ambient air pollution associations with DNA methylation in asthma- and allergy-relevant genes: findings from ECHO.","authors":"Meredith Palmore, Emma E Thompson, Fang Fang, Theresa M Bastain, Carrie Breton, Scott Collingwood, Frank D Gilliland, Diane R Gold, Rima Habre, Tina Hartert, Gurjit K Khurana Hershey, Daniel J Jackson, Rachel Miller, Patrick Ryan, Lyndsey Shorey-Kendrick, Eliot R Spindel, Joseph Stanford, James Gern, Chris McKennan, Carole Ober, Christine Ladd-Acosta","doi":"10.1093/eep/dvaf013","DOIUrl":"10.1093/eep/dvaf013","url":null,"abstract":"<p><p>Prenatal exposure to air pollution is an important risk factor for child health outcomes, including asthma. Identification of DNA methylation changes associated with air pollutant exposure can provide new intervention targets to improve children's health. The aim of this study is to test the association between prenatal air pollutant exposure and DNA methylation in developmental and asthma-/allergy-relevant biospecimens (placenta, buccal, cord blood, nasal mucosa, and lavage). A subset of 2294 biospecimens collected from 1906 child participants enrolled in the Environmental Influences on Child Health Outcomes program with prenatal air pollutant and high-quality Illumina Asthma&Allergy DNA methylation array measures (<i>n</i> = 37 197 probes) were included. Prenatal ozone, nitrogen dioxide, and fine particulate matter were derived using residential history during pregnancy and spatiotemporal models. For each pollutant, biospecimen type, and prenatal exposure window, we estimated the effects of air pollution on gene DNA methylation levels. We compared results across pollutants, biospecimen types, and trimesters and tested for critical months of exposure using distributed lag models. DNA methylation levels at 154 out of 4746 tested genes were associated with air pollution; over 95% were exposure window, pollutant, and biospecimen-type specific. The fewest gene associations were detected in trimester 2, relative to other exposure windows. A variety of trends in methylation patterns were observed in response to lagged monthly pollution levels. Child DNA methylation changes at specific respiratory- and immune-relevant genes are associated with prenatal air pollutant exposures. Future studies should examine the relationship between these pollution-sensitive genes and child health.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf013"},"PeriodicalIF":3.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global maintenance of histone post-translational modifications during the transition into anoxia in embryos of the annual killifish <i>Austrofundulus limnaeus</i>.","authors":"Chelsea Hughes, Elizabeth A Mojica, Dietmar Kültz, Jason E Podrabsky","doi":"10.1093/eep/dvaf015","DOIUrl":"10.1093/eep/dvaf015","url":null,"abstract":"<p><p>Many organisms have adapted to survive anoxic or hypoxic environments, but the epigenetic responses involved in this successful stress response are not well described in most species. Embryos of the annual killifish <i>Austrofundulus limnaeus</i> have the greatest tolerance to anoxia of all vertebrates, making them a powerful model to study the cellular mechanisms necessary for anoxia tolerance. However, the global histone landscape of this species has never been quantified or explored in relation to stress tolerance. Liquid chromatography-mass spectrometry and a Python bioinformatics workflow were used to identify histones and their post-translational modifications. This pipeline resulted in the detection of 252 unique biologically relevant histone post-translational modifications (hPTMs) (unimod + residue). These PTMs represent 16 types of biologically relevant hPTMs present during both anoxia and normoxia in Wourms' stage 36 embryos. This hPTM library presents an exciting opportunity to study histone modifications across development and in response to environmental stressors. No significant changes in PTM or histone abundance were observed between anoxic and normoxic embryos, suggesting that 24 h of anoxia is not sufficient to induce epigenetic or histone isoform changes at the organismal level. This result is inconsistent with data presented for similar stresses in mammalian cells and thus stabilization of the hPTM landscape may be an adaptation that supports anoxia tolerance.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf015"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics as the missing piece in epigenetics research.","authors":"Thomas Kenney, Tony Montina, Gerlinde A S Metz","doi":"10.1093/eep/dvaf014","DOIUrl":"10.1093/eep/dvaf014","url":null,"abstract":"","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf014"},"PeriodicalIF":3.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145430648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA methylation patterns linked to salinity and geography in the American eel (<i>Anguilla rostrata</i>).","authors":"Gabriela Ulmo Díaz, Céline Audet, Eric Normandeau, Clare J Venney, Kyle Wellband, Julie Turgeon, Louis Bernatchez","doi":"10.1093/eep/dvaf012","DOIUrl":"10.1093/eep/dvaf012","url":null,"abstract":"<p><p>The panmictic American eel (<i>Anguilla rostrata</i>) displays a wide range of intraspecific phenotypic variation as well as geographical sex bias and differential recruitment. By definition, panmictic species lack genetic structure, thus local adaptation through genetic variation cannot explain the presence of intraspecific variation. As a result, the contrasting phenotypes observed in the American eel could be attributed to either spatially varying selection, phenotypic plasticity (often mediated through epigenetic changes), or the interaction of both processes. Here we explore, for the first time, the role of DNA methylation in acclimatization in a panmictic species, the American eel, as well as its association with salinity and geography in Northeastern Canada. Using whole genome bisulfite sequencing in 72 individuals, we found that DNA methylation patterns were associated with geography and to a lesser degree with salinity. We identified a genomic region with differential methylation associated with salinity that falls inside the <i>SOCS2</i> gene, which has been previously linked to salinity differences in other fish species, as well as to metabolism and somatic growth regulation. This study advances our understanding of how panmictic species or populations with high gene flow acclimatize to variable environments in the absence of heritable genetic local adaptation.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf012"},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12684172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145713873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal per- and polyfluoroalkyl substance exposures and DNA methylation among newborns in the Environmental influences on Child Health Outcomes program.","authors":"Rose Schrott, Christine Ladd-Acosta, Vasantha Padmanabhan, Dana Boyd Barr, Carrie V Breton, Andres Cardenas, Courtney C Carignan, Dana Dabelea, Anne L Dunlop, Danielle M Fallin, Marie-France Hivert, Ellen M Howerton, Anna K Knight, Emily Oken, Alicia K Peterson, Michael C Petriello, Douglas Ruden, Rebecca J Schmidt, Alicia K Smith, Anne P Starling, Ivana V Yang, Yeyi Zhu, Jaclyn M Goodrich","doi":"10.1093/eep/dvaf010","DOIUrl":"10.1093/eep/dvaf010","url":null,"abstract":"<p><p>Gestation is a vulnerable window when exposure to per- and polyfluoroalkyl substances (PFAS) may impact child development and health. Epigenetic modification, including DNA methylation (DNAm), may be one mechanism linking prenatal PFAS exposure to offspring outcomes. We tested associations between prenatal PFAS and newborn DNAm in 1017 participants from 6 cohorts in the US Environmental influences on Child Health Outcomes consortium. Concentrations of PFAS [perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorohexanesulfonic acid (PFHxS), perfluorononanoic acid (PFNA), and perfluorodecanoic acid] were measured in maternal serum or plasma. DNAm was quantified in newborn dried blood spot or umbilical cord blood leukocytes using the Infinium HumanMethylation450 (450K) or MethylationEPIC (EPIC) arrays. We tested associations between prenatal PFAS and neonatal blood DNAm on the 450K (<i>n</i> = 772) and EPIC (<i>n</i> = 245) arrays; results were meta-analysed across the platforms. Regional changes in DNAm were investigated, and findings were checked for replication in the Michigan Mother-Infant Pairs (MMIP) cohort (<i>n</i> = 140). Following correction for false discovery rate (<i>q</i> = 0.1 for meta-analyses), we identified an association between PFHxS and one cytosine-guanine (CpG) mapped to <i>CASC3</i> (<i>q</i> = 0.065) that replicated in MMIP (<i>P</i> = .006). PFOS was associated with six CpG sites, of which five were mapped to the genes <i>KIAA1841, ABR, LEP, SERPINA1</i>, and <i>LOXL1</i>. One differentially methylated region (DMR) was associated with prenatal PFOA exposure, and one DMR was associated with PFOS exposure. In this multicohort analysis including a diverse group from the USA, PFOA, PFOS, PFHxS, and PFNA exposures in pregnancy were associated with offspring DNAm, and the implications for children's health merit further exploration.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf010"},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of mercury exposure and DNA methylation with sustained attention in children in a novel analysis of epigenetic susceptibility.","authors":"Jessa V Ehlinger, Jaclyn M Goodrich, Dana C Dolinoy, Deborah J Watkins, Alejandra Cantoral, Adriana Mercado-García, Niladri Basu, Martha M Téllez-Rojo, Karen E Peterson","doi":"10.1093/eep/dvaf011","DOIUrl":"10.1093/eep/dvaf011","url":null,"abstract":"<p><p>The etiology of attention-deficit/hyperactivity disorder (ADHD) remains poorly understood, despite it being one of the most common neurodevelopmental disorders worldwide. Past research suggests methylmercury exposure and DNA methylation (DNAm) levels are each associated with ADHD in children, yet whether they interact to affect ADHD is unknown. Leveraging data from a longitudinal cohort of children in Mexico, this novel epigenetic-environment interaction study identified significant interactions between childhood mercury exposure (measured at 6-12 years of age) and adolescent blood leukocyte DNAm in their association with sustained attention [quantified via the Conners continuous performance test, 3rd edition (CPT3)] measured on average 5.6 ± 0.99 years later. Using adjusted linear regression, we assessed associations between hair and urine mercury concentrations and CPT3 scores reflecting inattention, impulsivity, vigilance, and sustained attention (<i>N</i> = 399). We then tested the interaction between mercury and DNAm at loci previously associated with the CPT3 outcomes (<i>N</i> = 374). Significant associations between mercury and CPT3 differed in magnitude and direction depending on the mercury biomarker and CPT3 variable. These associations often differed by gender. For example, urine mercury was positively associated with vigilance scores in males [β = 1.31(SE = 0.65), <i>P</i> = .045] but not in females [β = -0.20 (SE = 0.81), <i>P</i> = .80). In all children, three significant mercury-DNAm interactions were identified for either inattention or vigilance outcomes. Among females, 155 significant interaction terms were identified for the inattention models. In males, three significant interactions were identified for the impulsivity model. Overall, results suggest in some cases DNAm can influence the association between mercury exposure and ADHD-like symptoms.</p>","PeriodicalId":11774,"journal":{"name":"Environmental Epigenetics","volume":"11 1","pages":"dvaf011"},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}