Enzyme and Microbial Technology最新文献_第9页

Surface expression of carbonic anhydrase on E. coli as a sustainable approach for enzymatic CO2 capture 碳酸酐酶在大肠杆菌上的表面表达作为一种可持续的二氧化碳酶捕集方法

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-17 DOI: 10.1016/j.enzmictec.2024.110422

Juned Ali , Shazia Faridi , Amuliya Kashyap , Shabnam , Rubia Noori , Meryam Sardar

{"title":"Surface expression of carbonic anhydrase on E. coli as a sustainable approach for enzymatic CO2 capture","authors":"Juned Ali , Shazia Faridi , Amuliya Kashyap , Shabnam , Rubia Noori , Meryam Sardar","doi":"10.1016/j.enzmictec.2024.110422","DOIUrl":"https://doi.org/10.1016/j.enzmictec.2024.110422","url":null,"abstract":"<div>The utilisation of carbonic anhydrase (CA) in CO2 sequestration is becoming prominent as an efficient, environment friendly and rapid catalyst for capturing CO2 from industrial emissions. However, the application of CA enzyme in soluble form is constrained due to its poor stability in operational conditions of CO2 capture and also production cost of the enzyme. Addressing these limitations, the present study focuses on the surface display of CA from Bacillus halodurans (BhCA) on E coli aiming to contribute to the cost-effectiveness of carbon capture through CA technology. This involved the fusion of the BhCA-encoding gene with the adhesion molecule involved in diffuse adherence (AIDA-I) autotransporter, resulting in the efficient display of BhCA (595 ± 60 U/gram dry cell weight). Verification of the surface display of BhCA was accomplished by conjugating with FITC labelled anti-his antibody followed by fluorescence-activated cell sorting (FACS) and cellular fractionation in conjunction with zymography. Biochemical characterisation of whole-cell biocatalyst revealed a noteworthy enhancement in thermostability, improvement in the thermostability with T1/2 of 90 ± 1.52 minutes at 50 ˚C, 36 ± 2.51 minutes at 60 ˚C and18 ± 1.52 minutes at 80˚C. Surface displayed BhCA displayed remarkable reusability retaining 100% activity even after 15 cycles. Surface displayed BhCA displayed highly alkali stable nature like free counterpart in solution. The alkali stability of the surface-displayed BhCA was comparable to its free counterpart in solution. Furthermore, the study investigated the impact of different metal ions, modulators, and detergents on the whole-cell biocatalysts. The present work represents the first report on surface display of CA utilising the AIDA-1 autotransporter.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"176 ","pages":"Article 110422"},"PeriodicalIF":3.4,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139945270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Economical one-pot synthesis of isoquercetin and D-allulose from quercetin and sucrose using whole-cell biocatalyst 利用全细胞生物催化剂从槲皮素和蔗糖经济地一步合成异槲皮素和 D-阿洛酮糖

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-15 DOI: 10.1016/j.enzmictec.2024.110412

Qi-Yang Wang, Hao-Yu Wang, Wei-Guo Zhang, Jian-Zhong Xu

{"title":"Economical one-pot synthesis of isoquercetin and D-allulose from quercetin and sucrose using whole-cell biocatalyst","authors":"Qi-Yang Wang, Hao-Yu Wang, Wei-Guo Zhang, Jian-Zhong Xu","doi":"10.1016/j.enzmictec.2024.110412","DOIUrl":"10.1016/j.enzmictec.2024.110412","url":null,"abstract":"<div>Isoquercetin and D-allulose have diverse applications and significant value in antioxidant, antibacterial, antiviral, and lipid metabolism. Isoquercetin can be synthesized from quercetin, while D-allulose is converted from D-fructose. However, their production scale and overall quality are relatively low, leading to high production costs. In this study, we have devised a cost-effective one-pot method for biosynthesizing isoquercetin and D-allulose using a whole-cell biocatalyst derived from quercetin and sucrose. To achieve this, the optimized isoquercetin synthase and D-allulose-3-epimerase were initially identified through isofunctional gene screening. In order to reduce the cost of uridine diphosphate glucose (UDPG) during isoquercetin synthesis and ensure a continuous supply of UDPG, sucrose synthase is introduced to enable the self-circulation of UDPG. At the same time, the inclusion of sucrose permease was utilized to successfully facilitate the catalytic production of D-allulose in whole cells. Finally, the recombinant strain BL21/UGT-SUS+DAE-SUP, which overexpresses MiF3GTMUT, GmSUS, EcSUP, and DAEase, was obtained. This strain co-produced 41±2.4 mg/L of isoquercetin and 5.7±0.8 g/L of D-allulose using 120 mg/L of quercetin and 20 g/L of sucrose as substrates for 5 h after optimization. This is the first green synthesis method that can simultaneously produce flavonoid compounds and rare sugars. These findings provide valuable insights and potential for future industrial production, as well as practical applications in factories.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"176 ","pages":"Article 110412"},"PeriodicalIF":3.4,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139814628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomanufacture of L-homoserine lactone building block: A strategy for preparing γ-substituted L-amino acids by modular reaction L-高丝氨酸内酯结构单元的生物制造：通过模块化反应制备γ-取代的 L-氨基酸的策略

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-07 DOI: 10.1016/j.enzmictec.2024.110411

Yuguang Wang , Mengjing Wu , Huifang Zheng , Dongmei Wu , Panpan Yao , Wenjing Li , Kexin Jin , Xinjun Yu

引用次数: 0

Efficient biosynthesis of prunin in methanol cosolvent system by an organic solvent-tolerant α-L-rhamnosidase from Spirochaeta thermophila 耐有机溶剂的α-L-鼠李糖酶在甲醇共溶剂体系中高效合成梅花苷

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-06 DOI: 10.1016/j.enzmictec.2024.110410

Chen-Mu Luo , Li-Fan Ke , Xiang-Yu Huang , Xiao-Yan Zhuang , Ze-Wang Guo , Qiong Xiao , Jun Chen , Fu-Quan Chen , Qiu-Ming Yang , Yi Ru , Hui-Fen Weng , An-Feng Xiao , Yong-Hui Zhang

{"title":"Efficient biosynthesis of prunin in methanol cosolvent system by an organic solvent-tolerant α-L-rhamnosidase from Spirochaeta thermophila","authors":"Chen-Mu Luo , Li-Fan Ke , Xiang-Yu Huang , Xiao-Yan Zhuang , Ze-Wang Guo , Qiong Xiao , Jun Chen , Fu-Quan Chen , Qiu-Ming Yang , Yi Ru , Hui-Fen Weng , An-Feng Xiao , Yong-Hui Zhang","doi":"10.1016/j.enzmictec.2024.110410","DOIUrl":"10.1016/j.enzmictec.2024.110410","url":null,"abstract":"<div>Prunin of desirable bioactivity and bioavailability can be transformed from plant-derived naringin by the key enzyme α-L-rhamnosidase. However, the production was limited by unsatisfactory properties of α-L-rhamnosidase such as thermostability and organic solvent tolerance. In this study, biochemical characteristics, and hydrolysis capacity of a novel α-L-rhamnosidase from Spirochaeta thermophila (St-Rha) were investigated, which was the first characterized α-L-rhamnosidase for Spirochaeta genus. St-Rha showed a higher substrate specificity towards naringin and exhibited excellent thermostability and methanol tolerance. The Km of St-Rha in the methanol cosolvent system was decreased 7.2-fold comparing that in the aqueous phase system, while kcat/Km value of St-Rha was enhanced 9.3-fold. Meanwhile, a preliminary conformational study was implemented through comparative molecular dynamics simulation analysis to explore the mechanism underlying the methanol tolerance of St-Rha for the first time. Furthermore, the catalytic ability of St-Rha for prunin preparation in the 20% methanol cosolvent system was explored, and 200 g/L naringin was transformed into 125.5 g/L prunin for 24 h reaction with a corresponding space-time yield of 5.2 g/L/h. These results indicated that St-Rha was a novel α-L-rhamnosidase suitable for hydrolyzing naringin in the methanol cosolvent system and provided a better alternative for improving the efficient production yield of prunin.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"175 ","pages":"Article 110410"},"PeriodicalIF":3.4,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139715909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the enzymatic production of biolubricants by the Taguchi method: Esterification of the free fatty acids from castor oil with 2-ethyl-1-hexanol catalyzed by Eversa Transform 2.0 用田口方法优化生物润滑剂的酶法生产：用 Eversa Transform 2.0 将蓖麻油中的游离脂肪酸与 2-乙基-1-己醇酯化

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-05 DOI: 10.1016/j.enzmictec.2024.110409

Rodolpho R.C. Monteiro , Maria M.F. de Melo Neta , Wesley S. Rocha , Jorge B. Soares , F. Murilo T. de Luna , Roberto Fernandez-Lafuente , Rodrigo S. Vieira

{"title":"Optimizing the enzymatic production of biolubricants by the Taguchi method: Esterification of the free fatty acids from castor oil with 2-ethyl-1-hexanol catalyzed by Eversa Transform 2.0","authors":"Rodolpho R.C. Monteiro , Maria M.F. de Melo Neta , Wesley S. Rocha , Jorge B. Soares , F. Murilo T. de Luna , Roberto Fernandez-Lafuente , Rodrigo S. Vieira","doi":"10.1016/j.enzmictec.2024.110409","DOIUrl":"10.1016/j.enzmictec.2024.110409","url":null,"abstract":"<div>The solvent-free esterification of the free fatty acids (FFAs) obtained by the hydrolysis of castor oil (a non-edible vegetable oil) with 2-ethyl-1-hexanol (a branched fatty alcohol) was catalyzed by different free lipases. Eversa Transform 2.0 (ETL) features surpassed most commercial lipases. Some process parameters were optimized by the Taguchi method (L16′). As a result, a conversion over 95% of the FFAs of castor oil into esters with lubricants properties was achieved under optimized reaction conditions (15 wt% of biocatalyst content, 1:4 molar ratio (FFAs/alcohol), 30 °C, 180 rpm, 96 h). The substrates molar ratio had the highest influence on the dependent variable (conversion at 24 h). FFAs/2-ethyl-1-hexanol esters were characterized regarding the physicochemical and tribological properties. Interestingly, the modification of the FFAs with 2-ethyl-1-hexanol by ETL increased the oxidative stability of the FFAs feedstock from 0.18 h to 16.83 h. The biolubricants presented a lower friction coefficient than the reference commercial mineral lubricant (0.052 ± 0.07 against 0.078 ± 0.04). Under these conditions, ETL catalyzed the oligomerization of ricinoleic acid (a hydroxyl fatty acid) into estolides, reaching a conversion of 25.15% of the initial FFAs (for the first time).</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"175 ","pages":"Article 110409"},"PeriodicalIF":3.4,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0141022924000164/pdfft?md5=542b6b7adc9e73368924c7f70ca4fa1a&pid=1-s2.0-S0141022924000164-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139689411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomanufacture of L-homoserine lactone building block: A strategy for preparing γ-substituted L-amino acids by modular reaction L-高丝氨酸内酯结构单元的生物制造：通过模块化反应制备γ-取代的 L-氨基酸的策略

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-01 DOI: 10.1016/j.enzmictec.2024.110411

Yuguang Wang, Mengjing Wu, Huifang Zheng, Dongmei Wu, Panpan Yao, Wenjing Li, Kexin Jin, Xinjun Yu

引用次数: 0

Preliminary identification and semi-quantitative characterization of a multi-faceted high-stability alginate lyase from marine microbe Seonamhaeicola algicola with anti-biofilm effect on Pseudomonas aeruginosa 海洋微生物 Seonamhaeicola algicola 中具有抗铜绿假单胞菌生物膜作用的多元高稳定性藻酸盐裂解酶的初步鉴定和半定量表征

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-01 DOI: 10.1016/j.enzmictec.2024.110408

Shuaiting Yun, Jinping Huang, Mingjing Zhang, Xueting Wang, Xiaochen Wang, Yanxia Zhou

{"title":"Preliminary identification and semi-quantitative characterization of a multi-faceted high-stability alginate lyase from marine microbe Seonamhaeicola algicola with anti-biofilm effect on Pseudomonas aeruginosa","authors":"Shuaiting Yun, Jinping Huang, Mingjing Zhang, Xueting Wang, Xiaochen Wang, Yanxia Zhou","doi":"10.1016/j.enzmictec.2024.110408","DOIUrl":"10.1016/j.enzmictec.2024.110408","url":null,"abstract":"<div>Alginate lyases with unique characteristics for degrading alginate into size-defined oligosaccharide fractions, were considered as the potential agents for disrupting Pseudomonas aeruginosa biofilms. In our study, a novel endolytic PL-7 alginate lyase, named AlyG2, was cloned and expressed through Escherichia coli. This enzyme exhibited excellent properties: it maintained more than 85% activity at low temperatures of 4 °C and high temperatures of 70 °C. After 1 h of incubation at 4 °C, it still retained over 95% activity, demonstrating the ability to withstand low temperature. The acid-base and salt tolerance properties shown it preserves more than 50% activity in the pH range of 5.0 to 11.0 and in a high salt environment at 3000 mM NacCl, indicating its high stability in several aspects. More importantly, AlyG2 in our research was revealed to be effective at removing mature biofilms and inhibiting biofilm formation produced by Pseudomonas aeruginosa, and the inhibition and disruption rates were 47.25 ± 4.52% and 26.5 ± 6.72%, respectively. Additionally, the enzyme AlyG2 promoted biofilm disruption in combination with antibiotics, particularly manifesting the synergistic effect with erythromycin (FIC=0.5). In all, these results offered that AlyG2 with unique characteristics may be an effective technique for the clearance or disruption of biofilm produced by P. aeruginosa.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"175 ","pages":"Article 110408"},"PeriodicalIF":3.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139657982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Economical one-pot synthesis of isoquercetin and D-allulose from quercetin and sucrose using whole-cell biocatalyst 利用全细胞生物催化剂从槲皮素和蔗糖经济地一步合成异槲皮素和 D-阿洛酮糖

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-02-01 DOI: 10.1016/j.enzmictec.2024.110412

Qi-Yang Wang, Hao-Yu Wang, Wei-Guo Zhang, Jian-Zhong Xu

引用次数: 0

Application of extremophile cell factories in industrial biotechnology 嗜极细胞工厂在工业生物技术中的应用

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-01-30 DOI: 10.1016/j.enzmictec.2024.110407

Yuzhou Wang , Jinyi Qian , Tianqiong Shi , Yuetong Wang , Qiang Ding , Chao Ye

{"title":"Application of extremophile cell factories in industrial biotechnology","authors":"Yuzhou Wang , Jinyi Qian , Tianqiong Shi , Yuetong Wang , Qiang Ding , Chao Ye","doi":"10.1016/j.enzmictec.2024.110407","DOIUrl":"10.1016/j.enzmictec.2024.110407","url":null,"abstract":"<div>Due to the extreme living conditions, extremophiles have unique characteristics in morphology, structure, physiology, biochemistry, molecular evolution mechanism and so on. Extremophiles have superior growth and synthesis capabilities under harsh conditions compared to conventional microorganisms, allowing for unsterilized fermentation processes and thus better performance in low-cost production. In recent years, due to the development and optimization of molecular biology, synthetic biology and fermentation technology, the identification and screening technology of extremophiles has been greatly improved. In this review, we summarize techniques for the identification and screening of extremophiles and review their applications in industrial biotechnology in recent years. In addition, the facts and perspectives gathered in this review suggest that next-generation industrial biotechnology (NGIBs) based on engineered extremophiles holds the promise of simplifying biofuturing processes, establishing open, non-sterilized continuous fermentation production systems, and utilizing low-cost substrates to make NGIBs attractive and cost-effective bioprocessing technologies for sustainable manufacturing.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"175 ","pages":"Article 110407"},"PeriodicalIF":3.4,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139645493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ω-transaminase-catalyzed synthesis of (R)-2-(1-aminoethyl)-4-fluorophenol, a chiral intermediate of novel anti-tumor drugs ω-反式胺酶催化合成(R)-2-(1-氨基乙基)-4-氟苯酚--新型抗肿瘤药物的手性中间体

IF 3.4 3区生物学

Enzyme and Microbial Technology Pub Date : 2024-01-26 DOI: 10.1016/j.enzmictec.2024.110406

Quan Luo , Guan Zhou , Zhongxia Li , Jiangpeng Dong , Hang Zhao , Huifang Xu , Xuefeng Lu

{"title":"ω-transaminase-catalyzed synthesis of (R)-2-(1-aminoethyl)-4-fluorophenol, a chiral intermediate of novel anti-tumor drugs","authors":"Quan Luo , Guan Zhou , Zhongxia Li , Jiangpeng Dong , Hang Zhao , Huifang Xu , Xuefeng Lu","doi":"10.1016/j.enzmictec.2024.110406","DOIUrl":"10.1016/j.enzmictec.2024.110406","url":null,"abstract":"<div>The chiral amine (R)-2-(1-aminoethyl)-4-fluorophenol has attracted increasing attentions in recent years in the field of pharmaceuticals because of its important use as a building block in the synthesis of novel anti-tumor drugs targeting tropomyosin receptor kinases. In the present study, a ω-transaminase (ωTA) library consisting of 21 (R)-enantioselective enzymes was constructed and screened for the asymmetric biosynthesis of (R)-2-(1-aminoethyl)-4-fluorophenol from its prochiral ketone. Using (R)-α-methylbenzylamine, D-alanine, or isopropylamine as amino donor, 18 ωTAs were identified with target activity and the enzyme AbTA, which was originally identified from Arthrobacter sp. KNK168, was found to be a potent candidate. The E. coli whole cells expressing AbTA could be used as catalysts. The optimal temperature and pH for the activity were 35–40 °C and pH8.0, respectively. Simple alcohols (such as ethanol, isopropanol, and methanol) and dimethyl sulfoxide were shown to be good cosolvents. High activities were detected when using ethanol and dimethyl sulfoxide at the concentrations of 5–20%. In the scaled-up reaction of 1-liter containing 13 mM ketone substrate, about 50% conversion was achieved in 24 h. 6.4 mM (R)-2-(1-aminoethyl)-4-fluorophenol was generated. After a simple and efficient process of product isolation and purification (with 98.8% recovery), 0.986 g yellowish powder of the product (R)-2-(1-aminoethyl)-4-fluorophenol with high (R)-enantiopurity (up to 100% enantiomeric excess) was obtained. This study established an overall process for the biosynthesis of the high value pharmaceutical chiral amine (R)-2-(1-aminoethyl)-4-fluorophenol by ωTA. Its applicable potential was exemplified by gram-scale production.</div>","PeriodicalId":11770,"journal":{"name":"Enzyme and Microbial Technology","volume":"175 ","pages":"Article 110406"},"PeriodicalIF":3.4,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139586365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0