Ejc Supplements最新文献_第8页

P64: Effect of polyclonal activators on cytokine-producing capacity of blood immunocompetent cells in adenoma and adenocarcinoma of the stomach P64:多克隆激活剂对胃腺瘤和胃腺癌血液免疫活性细胞产生细胞因子能力的影响

Ejc Supplements Pub Date : 2015-11-01 DOI: 10.1016/J.EJCSUP.2015.08.101

A. V. Sosnina, A. V. Vankhalsky, E. Mikhailova, T. A. Kunts, N. Varaksin, A. Autenshlyus

引用次数: 0

P61: DNA-vaccines against melanoma: Design and investigation of antigenic properties 抗黑色素瘤的dna疫苗:抗原特性的设计和研究

Ejc Supplements Pub Date : 2015-11-01 DOI: 10.1016/J.EJCSUP.2015.08.103

E. Starostina, D. Antonets, E. A. Borobova, L. Karpenko, A. Reguzova, O. Smirnova, A. Ilyichev, Bazhan Si

引用次数: 0

A36: The TP53 mutations in the Russian patients with de novo DLBCL A36:俄罗斯新发DLBCL患者的TP53突变

Ejc Supplements Pub Date : 2015-11-01 DOI: 10.1016/J.EJCSUP.2015.08.117

E. Voropaeva, T. Pospelova, Voevoda Mi, V. Maximov

引用次数: 0

Title page 2 第2页

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/S1359-6349(15)00134-2

引用次数: 0

P82a P82a:分子生物标志物在甲状腺癌术前诊断中的应用

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.093

S. Shevchenko , L. Mostovich , N. Kolesnikov , L. Gulyaeva

{"title":"P82a","authors":"S. Shevchenko , L. Mostovich , N. Kolesnikov , L. Gulyaeva","doi":"10.1016/j.ejcsup.2015.08.093","DOIUrl":"10.1016/j.ejcsup.2015.08.093","url":null,"abstract":"<div>Preoperative differential diagnosis of benign thyroid nodules and thyroid cancer is an important issue of endocrinology. Preoperative identification of a tumor type allows a surgeon to determine an adequate surgical treatment and reduce complications. The cytological analysis of samples obtained by the fine-needle aspiration biopsy is limited in sensitivity and specificity and the core biopsy is not safe to be used in patients with thyroid nodes smaller than 2cm. The most promising area in the preoperative diagnosis of malignant tumors is molecular biomarkers, which can be used to determine the surgical treatment and indications for the target therapy. BRAF V600E is the most frequent genetic alteration in thyroid cancer. It activates the MAP-kinase pathway which causes changes in expression levels of extracellular matrix proteins and some of their receptors. Changes in the expression of integrin receptors and their ligands, such as osteopontin and thrombospondin-1, contribute to tumor cell proliferation and migration. The C-Jun pathway is also very important in pathogenesis of thyroid cancer. Changes in its activity can affect the expression of GSTP enzyme which participates in hormone metabolism. Altered MiRNA expression has been observed in a variety of cancer states allowing their potential use as cancer biomarkers.The aim of this study was to evaluate integrins, angiogenic factors, GSTP and MiRNAs as potential biomarkers for the diagnosis and prognosis of thyroid cancer.112 samples of papillary thyroid cancer (PTC) and 120 samples of benign nodular neoplasms were analyzed. The expression levels of the studied genes were determined by RT-PCR. The results were confirmed by immunohistochemical analysis. The BRAF V600E mutation was determined by allele-specific real-time PCR. The results showed that GSTP can be used for clinical settings as a cancer-specific marker for thyroid neoplasms with 83–88% sensitivity, 70–80% specificity and 76–84% diagnostic accuracy. Higher expression levels of integrins α2, α5, αv, α9, β1, β3 and IL-8, angiogenin, and VEGF were observed in malignant tumors in comparison with the normal thyroid tissue (p  <0.05). The expression of miRNA 21, 221, 222, 155 was significantly increased in the cancer samples compared to the benign neoplasms. Thus, the studied molecular markers could be used for preoperative diagnosis of thyroid neoplasms and the treatment approach.</div>","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Page 52"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54310105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A139 A139

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.096

O. Shpileva

{"title":"A139","authors":"O. Shpileva","doi":"10.1016/j.ejcsup.2015.08.096","DOIUrl":"10.1016/j.ejcsup.2015.08.096","url":null,"abstract":"<div>Cervical cancer is one of the most common female malignancies with incidence of 19.7 per 100,000 population in Russia in 2011 (Davydov, Aksel et al., 2011). Over 6000 women in Russia die of cervical cancer annually.The cervical cancer incidence shows a tendency towards increasing rates among young women (Chissov, 2009). Tomsk region has been found to be the territory of increased cancer risk for cervical cancer. The age-standardized incidence rate is 1.87 times higher in Tomsk region than in Russia, being 20.40/0000 (Pisareva, Odintsova et al., 2012). The highest incidence of cervical cancer is observed in women aged 15–39years (Churuksaeva, Kolomiets, Shpileva, 2012). The causal role of human papillomavirus infections in cervical cancer has been documented beyond reasonable doubt. Prevention of exposure to high risk HPV types by vaccination may prove to be the most efficient and logistically feasible preventive intervention for cervical cancer.Epidemiological studies conducted at the Tomsk Cancer Research Institute have shown that the median age of patients with cervical intraepithelial neoplasia and cervical cancer is 39.9±8.5, and 89.5% of women are HPV-positive. Prevalence of high-grade squamous intraepithelial lesion (H-SIL) peaks between ages 25years and 30years.The predominant HPV type in screened women of Tomsk region as well as worldwide is HPV-16, reaching peak incidence in women aged 36–40 years (74%). In the older age group (from 51 to 60years), HPV-18 is associated with 25% of cervical cancer cases. High prevalence of HPV-31 has been found in women under the age of 45 years with an incidence peak (17%) in the age group ⩽20years.The geographical widespread data on HPV type-distribution are essential for estimating the impact of vaccines on cervical cancer and cervical screening programs. Immunization against HPV for young women aged between 9 and 26years, with a predominant age cohort 11–13years, was introduced in Tomsk region in 2010. The aim of the HPV immunization program is to protect females before they reach an age when the risk of HPV infection increases. A total of 627 girls were vaccinated, and 1653 doses of vaccines were injected. The three- dose schedule was given to 414 (66%) girls and 2-dose schedule to 198 (31.6%) girls. Vaccine safety assessment was carried out. Adverse effects were observed in 9.6% of cases and were mainly characterized by dizziness and pain at the injection site. Vaccination was well tolerated.When calculating socio-economic feasibility of the proposed technology , not only the economic damage caused by the high mortality of women from cervical cancer, but also the cost for treatment of precancerous cervical lesions were taken into account, as out of 25 women with undetected CINII-III, 10 will develop cervical cancer. There have been calculated the estimated dam","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Pages 53-54"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54310144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T93 T93

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.017

N. Cherdyntseva , M. Stakheyeva , N. Litviakov , M. Zavyalova , Y. Kukharev , J. Kzhyshkowska

{"title":"T93","authors":"N. Cherdyntseva , M. Stakheyeva , N. Litviakov , M. Zavyalova , Y. Kukharev , J. Kzhyshkowska","doi":"10.1016/j.ejcsup.2015.08.017","DOIUrl":"10.1016/j.ejcsup.2015.08.017","url":null,"abstract":"<div><h3>Background</h3>Risk of metastasis formation is provided by both tumor cell biological characteristics and the microenvironment features within the primary tumor along with local and systemic conditions for metastatic niche formation. The inflammatory infiltration has been shown to strongly impact on tumor progression (Whiteside, 2013). Dronca et al. (2011) showed that immunosuppressive factors in the tumor microenvironment may impair not only local immune responses but also disturb systemic immunity. Zitvogel et al. anticipate that the comprehension of the mechanisms governing the immunogenicity of cell death will have a profound impact on the design of anticancer therapies.To study the impact of immune system on clinical response to neoadjuvant chemotherapy and metastasis-free survival in breast cancer patients.</div><div><h3>Materials and methods</h3>350 patients with newly diagnosed invasive breast cancer treated with neoadjuvant chemotherapy (NAC) were enrolled into the study. The procedures were made in accordance with the Helsinki Declaration. Clinical response to chemotherapy, the 5-year metastasis-free survival and all major clinical and morphological parameters were determined. The original method of multidimensional data visualization was applied to present the immune system state as integral entirety in visual image for classification of patients with different risk of metastasis (NovoSpark Corporation, Canada). Copy number aberrations (CNA) of cytokine gene regions in tumor specimens were tested using high-density microarray platform CytoScanTM HD Array (Affymetrix, USA). Cytokine gene polymorphism was analyzed. Subpopulations of lymphocytes and macrophages were determined within the primary tumors by IHC.</div><div><h3>Results</h3>We found, that favorable clinical immediate response to preoperative chemotherapy was related to the high levels of IL-1beta, TNF-alpha and IL-10 production by peripheral mononuclear cells before the treatment. This correlation was further confirmed by data from the study on association between cytokine gene functional polymorphism and response to NAC. We used NovoSpark Corporation visualization approach allowing the representation the immune system state as integral unit and to discriminate breast cancer patients with high and low risk of haematogeneic metastasis. When estimated before cancer treatment, 95% of breast cancer patients had risk of metastasis. The neoadjuvant chemotherapy and surgical tumor removal reduced the risk of tumor progression to 62–71%. However, in a year after adjuvant chemo- and radiotherapy, the patient group with high risk of metastases increased to 81% again. Thus, the cancer treatment can change the primarily estimated outcome prognosis in breast cancer patients, and the monitoring of immune system is a promising approach to predict the risk of cancer progression or resistance to the therapy. We have found the connection between the profile of intra-tu","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Page 10"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54308814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P152 P152

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.036

S. Hofmann, I. Bure, A. Agaimy, A. Hartmann, F. Haller, E. Moskalev

{"title":"P152","authors":"S. Hofmann, I. Bure, A. Agaimy, A. Hartmann, F. Haller, E. Moskalev","doi":"10.1016/j.ejcsup.2015.08.036","DOIUrl":"10.1016/j.ejcsup.2015.08.036","url":null,"abstract":"<div>Aberrant alterations of DNA methylation patterns have been recognized as early and common events in oncogenesis emphasizing high therapeutic and preventive potential. Concurrent epigenetic silencing of multiple tumour suppressor genes has been reported in different tumours suggesting the existence of a directed program, whereby groups of genes are regulated by promoter methylation. The molecular bases of such a program remain largely unclear. Certain long non-coding RNAs (lncRNAs) have been recently identified that are responsible for target specificity of the histone modification complexes. It remains incompletely understood, however, if lncRNAs may play a role in patterning DNA methylation. In this study, we asked by using gastrointestinal stromal tumours as a model if an epigenetic related HOX Antisense Intergenic RNA, or HOTAIR, is involved in establishment of DNA methylation patterns. To this end, we first showed high up-regulation of HOTAIR in patient samples of high risk compared to low and intermediate risk groups (n  =6.7×10−6), what is supported by the earlier reports on common carcinomas. Highest levels of HOTAIR endogenous expression were next detected also in cell lines GIST T1, GIST48b and GIST882. Stable knockdown of HOTAIR was achieved in GIST T1 and GIST48b cells by RNAi using lentiviral transduction. As expected epigenetic alterations due to HOTAIR knockdown could develop with a delay, genome-wide DNA methylation profiling was performed at passage 12 after the transduction on the Infinium HumanMethylation450 BeadChip platform in triplicates. DNA methylation data were analysed by an R package RnBeads. A total of 218 CpG sites got hypomethylated upon HOTAIR knockdown in GIST T1 and GIST48b cells (Δβ    <!-->0.05). These included potential tumour suppressors, transcription factors, tumour-specific antigens, genes related to angiogenesis or involved in metabolism. As confirmed by using bisulfite pyrosequencing for a representative locus, DNA methylation of 64% degree at promoter associated CpG sites of the potential tumour suppressor RASSF1 was almost entirely erased upon HOTAIR knockdown in GIST T1 cell line with concomitant up-regulation detected by qRT-PCR. Concordant with earlier reports, HOTAIR knockdown led to reduced migration potential in GIST T1 cells. Taken together, the results suggest that HOTAIR is one of the factors involved in establishment of specific DNA methylation patterns in GIST. While the molecular mechanism remains to be determined, it is plausible to assume a recruitment of DNA methyltransferases by the Polycomb repressive complex 2, which target specificity is determined by HOTAIR. The results further suggest the feasibility of manipulating DNA methylat","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Pages 20-21"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54309173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P100 P100

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.071

E. Nikitina , O. Cheremisina , D. Kulbakin , N. Litviakov

{"title":"P100","authors":"E. Nikitina , O. Cheremisina , D. Kulbakin , N. Litviakov","doi":"10.1016/j.ejcsup.2015.08.071","DOIUrl":"10.1016/j.ejcsup.2015.08.071","url":null,"abstract":"<div>The purpose of the study was to access pattern of microRNA expression in tissue of precancerous lesions and larynx cancer.A total of 25 people with a diagnosis of dysplasia II–III (n  =15) and larynx cancer patients (n  =25) although a trend towards overexpression of oncogenic miRNA-21 and -155 according to severity of dysplastic changes in a tissue were present. Detailed analysis showed significant overexpression of that miRNAs as well as miRNA-200c and -205 in a group of dysplasia II- III against control group (15 vs 10, p  =0.045 and p  =0.038, respectively) but these results did not meet 5% FDR correction.Data showed overexpression of the same microRNA (-205, -155, -200 and -21) in larynx cancer patients compared to control group (46 vs 15, p  =0.008, p  =0.013, respectively). It should be pointed that microRNA pattern both in larynx cancer and patients with dysplasia II-III was very close showing similarity of these groups at molecular level. Frequency of cases with microRNA-205 overexpression was 2.98 times higher in cancer patients than in those who had no malignant transformation (CI 95%, 1.41–16.26, p    <!-->4.79). Three other microRNAs did not show any promising results as biomarkers of cancer progression but data obtained has interesting fundamental value.These miRs (-21, -155, -205 and -200c) are known to be regulators of processes that play an emergent role in carcinogenesis and our results obtained in vivo highlight and expand knowledge about some aspects of larynx cell transformation. Data suggest that miRNAs changing its expression according to dysplasia progress and could be important players in complex process of carcinogenesis as well as could be potential markers of disease progression.</div>","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Page 40"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54309818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P123 P123

Ejc Supplements Pub Date : 2015-11-01 Epub Date: 2015-11-23 DOI: 10.1016/j.ejcsup.2015.08.064

E. Matiunina , A. Kozlov , A. Emelyanov

{"title":"P123","authors":"E. Matiunina , A. Kozlov , A. Emelyanov","doi":"10.1016/j.ejcsup.2015.08.064","DOIUrl":"10.1016/j.ejcsup.2015.08.064","url":null,"abstract":"<div>The origin of evolutionary novel genes is connected with the origin and evolution of the novel organismal functions and the increase in morphological complexity of multicellular organisms. Novel gene acquire new or altered functions. Here we describe that evolutionary novel genes, expressed predominantly in transgenic fish tumors after their induced regression, determine progressive evolutionary characters and are conserved in human.We used a sample of genes, which were activated in inducible krasV12 transgenic zebrafish tumors according to the results of RNASeq data. Genes from our sample were expressed after HCC regression upon kras inactivation. The search of orthologs by different tools, such as blastx and psiblast, with e-value cut off not less than 10–3 and query coverage more than 50%, discovered that considerable proportion of tumor-specifically expressed genes are evolutionary novel, i.e. their orthologs are not found in Lamprey. Nine tenths of these novel genes have orthologs in humans. Gene ontology (GO) data were used to analyze their functions. According to the results of GO, some of the genes have functions not characteristic to fish. Among known functions of the human orthologues of evolutionary novel genes of zebrafish (vs. Lamprey) there are some important morphogenetic functions. For example, genes ccdc40, lmx1ba and lepa acquired functions in lung, embryonic utera and placenta, i.e. organs originated in taxa higher than fish. A large proportion of genes from our sample are connected with retina type eye, heart and brain development.</div>","PeriodicalId":11675,"journal":{"name":"Ejc Supplements","volume":"13 1","pages":"Page 36"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ejcsup.2015.08.064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54309718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1