Endothelium-journal of Endothelial Cell Research最新文献_第2页

Highlights of the 13th Endothelial Cell Research Symposium, Rotterdam, The Netherlands 第十三届内皮细胞研究研讨会，荷兰鹿特丹

Endothelium-journal of Endothelial Cell Research Pub Date : 2002-01-01 DOI: 10.1080/10623320212008

T. Hagen

引用次数: 0

Conference Report: 6th Biannual International Meeting "Angiogenesis: Basic Science and Clinical Development" 会议报告:第六届“血管生成:基础科学与临床发展”国际双年会

Endothelium-journal of Endothelial Cell Research Pub Date : 2002-01-01 DOI: 10.1080/10623320210713

P. Lelkes

引用次数: 0

Low-density lipoprotein induced actin cytoskeleton reorganization in endothelial cells: mechanisms of action. 低密度脂蛋白诱导内皮细胞肌动蛋白细胞骨架重组:作用机制。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109165321

J. Holland, R. Goss, R. O'donnell, M. Chang, D. K. Johnson, L. M. Ziegler

{"title":"Low-density lipoprotein induced actin cytoskeleton reorganization in endothelial cells: mechanisms of action.","authors":"J. Holland, R. Goss, R. O'donnell, M. Chang, D. K. Johnson, L. M. Ziegler","doi":"10.3109/10623320109165321","DOIUrl":"https://doi.org/10.3109/10623320109165321","url":null,"abstract":"The inhibitory effects of the specific NADPH oxidase inhibitor, apocynin, and non-specific NADPH oxidase inhibitors, nordihydroguaiaretic acid (NDGA) and SKF525A, on the disruption of dense peripheral bands and formation of stress fibers in cultured human umbilical vein endothelial cells exposed to atherogenic low-density lipoprotein (LDL) levels has been investigated. Endothelial cells (EC) in vitro and in vivo exposed to high LDL-cholesterol levels have cytoskeletal remodeling with stress fiber formation and loss of dense peripheral bands. Cultured EC incubated with exogenously applied hydrogen peroxide (H2O2: 1 mM) have cytoskeletal structural changes much similar to those observed with high LDL exposure. Previous studies have 1) demonstrated that exposure to atherogenic LDL levels causes heightened EC H2O2 production, 2) identified the reactive oxygen species source, NADPH oxidase, in EC, and 3) shown that the specific NADPH oxidase inhibitor, apocynin, and non-specific NADPH oxidase inhibitors, NDGA and SKF525A, suppress H2O2 production increases in high LDL-perturbed EC. In the present study, the cytoskeletal structure of EC exposed to 330 mg/dl LDL-cholesterol, and incubated with or without apocynin, NDGA and SKF525A, was examined. Each of these compounds promoted the retention of dense peripheral bands and minimized stress fiber formation. These findings are consistent with NADPH oxidase and it's reactive oxygen species byproducts modulating the cytoskeleton reorganization observed in high LDL-induced EC perturbation.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"32 1","pages":"117-35"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90554485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

TGF-beta1 induces an accumulation of connexin43 in a lysosomal compartment in endothelial cells. tgf - β 1诱导内皮细胞溶酶体腔室中连接蛋白43的积累。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109090802

D. M. Larson, T. Christensen, G. Sagar, E. Beyer

{"title":"TGF-beta1 induces an accumulation of connexin43 in a lysosomal compartment in endothelial cells.","authors":"D. M. Larson, T. Christensen, G. Sagar, E. Beyer","doi":"10.3109/10623320109090802","DOIUrl":"https://doi.org/10.3109/10623320109090802","url":null,"abstract":"We have been studying the relationships between cell growth and the expression of the gap junction protein Connexin43 (Cx43) in cultured bovine aortic endothelial cells (BAEC). As part of these studies, we examined the effect of the growth inhibitory cytokine TGF-beta1 on Cx43 expression. We have shown recently that TGF-beta treatment increases Cx43 mRNA and synthesis, content, and half-life of the protein within 24 h, which leads, over the course of days, to an accumulation of Cx43 in large, intensely immunostaining vesicles, filling much of the perinuclear cytoplasmic space. In the current study, based on their distribution and markers, we identified these vesicles as lysosomes/autophagosomes. Cx43 immunostaining and staining with a fluorescent probe for acidic compartments are coincident, as retention of a fluorescent-labeled low-density lipoprotein occurs in a similar pattern and the same staining pattern can be detected in the treated cells using other markers for lysosomal compartments. TEM revealed prominent lysosomal figures with considerable heterogeneous material. After withdrawal of TGF-beta, the accumulated Cx43 was cleared only slowly, with some brightly immunoreactive cells remaining even after 72 h. The prolonged appearance (based on immunoreactivity in situ and in immunoblots) of intact vesicular Cx43 in the treated cells suggests decreased degradation, resulting from impaired lysosomal activity. These data not only emphasize the importance of the lysosome in connexin degradation, but also show that TGF-beta can cause an alteration in lysosomal functioning, with implications for cellular metabolism.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"326 1","pages":"255-60"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75714443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Update on pulmonary edema: the role and regulation of endothelial barrier function. 肺水肿最新进展:内皮屏障功能的作用和调控。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109165319

Carolyn E. Patterson, Hazel Lum

引用次数: 46

Nitrobenzylthioinosine (NBT), a nucleoside transport inhibitor, protects against Shiga toxin cytotoxicity in human microvascular endothelial cells. 硝基苄基硫代肌苷(NBT)是一种核苷转运抑制剂，对人微血管内皮细胞的志贺毒素细胞毒性具有保护作用。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109090803

K. Ohmi, N. Kiyokawa, T. Sekino, T. Suzuki, K. Mimori, T. Taguchi, H. Nakajima, Y. Katagiri, J. Fujimoto, H. Nakao, T. Takeda

引用次数: 1

Lipid polarity in brain capillary endothelial cells. 脑毛细血管内皮细胞的脂质极性。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.1080/10623320109051566

B. Tewes, H. Franke

{"title":"Lipid polarity in brain capillary endothelial cells.","authors":"B. Tewes, H. Franke","doi":"10.1080/10623320109051566","DOIUrl":"https://doi.org/10.1080/10623320109051566","url":null,"abstract":"Brain capillary endothelial cells (BCEC) represent an epithelial like cell type with continuous tight junctions and polar distributed proteins. In this paper we investigated whether cultured BCEC show a polar distribution of membrane lipids as this was demonstrated for many epithelial cell types. Therefore we applied a high yield membrane fractionation method to isolate pure fractions of the apical and the basolateral plasma membrane (PM) domains. Using a set of methods for lipid analysis we were able to determine the total lipid composition of the whole cells and the PM fractions. Both membrane domains showed a unique lipid composition with clear differences to each other and to the whole cell composition. Three lipid species were polar distributed between the two PM domains. Phosphatidylcholine was enriched in the apical membrane whereas sphingomyelin and glucosylceramide were enriched in the basolateral membrane. The possible function of this lipid polarity for the blood-brain barrier mechanism is the generation of a suitable lipid environment for polar distributed membrane proteins and the generation of two PM domains with different biophysical properties and permeabilities.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"2 1","pages":"207-20"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90191750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Insulin preincubation effects on rat vessel contractile responses: role of the sarcoplasmic reticulum. 胰岛素对大鼠血管收缩反应的影响:肌浆网的作用。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109090805

A. Rebolledo, V. Milesi, J. Raingo, A. Gómez Alvis, G. Rinaldi, A. O. Grassi de Gende

{"title":"Insulin preincubation effects on rat vessel contractile responses: role of the sarcoplasmic reticulum.","authors":"A. Rebolledo, V. Milesi, J. Raingo, A. Gómez Alvis, G. Rinaldi, A. O. Grassi de Gende","doi":"10.3109/10623320109090805","DOIUrl":"https://doi.org/10.3109/10623320109090805","url":null,"abstract":"In the present work, we studied the possible mechanisms involved in the insulin-induced acceleration of ET1 contractions. We observed a shortening of the half-life needed to achieve maximal developed force (t(1/2)) at 10(-7) M ET1 in rat aortic rings preincubated for 120 min with 3 nM insulin (control 380 +/- 15 s vs. 319 +/- 8 s with insulin, n = 28, p < 0.05). A tyrosine kinase linked receptor was involved in this effect because it was abolished by 30 microM genistein. Endothelium denudation and 10 microM indomethacin treatment did not effect this insulin effect, suggesting its independence of endothelial-derived factors. The effect was still present when the only source of Ca2+ was intracellular (t(1/2) values in the absence of external Ca2+: control 467 +/- 68 s vs. 213 +/- 28 s with insulin, n = 16, p < 0.05), but was blunted if the sarcoplasmic reticulum (SR) Ca2+ source was suppressed by exposure to 10 microM thapsigargin or 10 microM ryanodine. Preincubation with insulin did not potentiate either SR 45Ca2+ uptake or contractions evoked by caffeine-sensitive SR Ca2+ release. Since 30 microM cheleritrine abolished insulin-induced acceleration of ET1 contractions, we propose that the hormone might enhance a signal pathway related to PKC in order to produce a faster Ca2+ release from the SR.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"80 1","pages":"277-82"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85767492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vascular endothelial growth factor in porcine-derived extracellular matrix. 猪源性细胞外基质中的血管内皮生长因子。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109063154

J. Hodde, R. Record, H. Liang, Steven F. Badylak

{"title":"Vascular endothelial growth factor in porcine-derived extracellular matrix.","authors":"J. Hodde, R. Record, H. Liang, Steven F. Badylak","doi":"10.3109/10623320109063154","DOIUrl":"https://doi.org/10.3109/10623320109063154","url":null,"abstract":"An extracellular matrix (ECM) derived from the submucosa of the porcine small intestine (SIS) has been shown to induce angiogenesis and host tissue remodeling when used as a xenogeneic bioscaffold in animal models of wound repair. In the present study, we compared the in vitro effects of SIS ECM extracts to several purified angiogenic growth factors on human dermal microvascular endothelial cell (HMEC) growth patterns. The SIS ECM was shown to induce tube formation from HMEC in a three-dimensional fibrin-based angiogenesis assay in a manner similar to that caused by the addition of vascular endothelial growth factor (VEGF). This tube formation was blocked in the presence of anti-VEGF neutralizing antibody. Western blots and ELISA procedures showed that the SIS ECM contains as much as 0.77 ng VEGF/g SIS. The closely related endothelial cell mitogen, platelet-derived growth factor (PDGF), was not detectable in the SIS extracts. We conclude that VEGF is present in the SIS extracellular matrix. The role of VEGF in SIS-induced wound repair remains unknown, but its presence in the ECM makes it a possible contributor to the angiogenic effect of SIS when this ECM is used as a tissue repair scaffold in animal models of wound repair.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"131 1","pages":"11-24"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85137662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 332

Mechanotransduction of endothelial oxidative stress induced by cyclic strain. 循环应变诱导内皮氧化应激的机械转导。

Endothelium-journal of Endothelial Cell Research Pub Date : 2001-01-01 DOI: 10.3109/10623320109090806

David S Wang, David Proffit, P. Tsao

{"title":"Mechanotransduction of endothelial oxidative stress induced by cyclic strain.","authors":"David S Wang, David Proffit, P. Tsao","doi":"10.3109/10623320109090806","DOIUrl":"https://doi.org/10.3109/10623320109090806","url":null,"abstract":"Atherosclerotic lesions display a nonuniform distribution throughout the vascular tree. Mechanical forces produced by local alterations in blood flow may play an important role in the localization of atherosclerosis. One such force, cyclic strain, has been hypothesized to promote atherogenesis by inducing oxidative stress in endothelial cells, resulting in enhanced endothelial adhesiveness for monocytes. To investigate the signal transduction systems involved, human aortic endothelial cells were plated on flexible silicone strips that were either non-coated or adsorbed with poly-L-lysine, vitronectin, fibronectin, or collagen I. Cells were then subjected to uniform sinusoidal stretch (10%) for 6 h. Endothelial superoxide anion production was increased in cells exposed to cyclic strain compared to static conditions. Furthermore, endothelial oxidative response to stretch was matrix protein-dependent, whereas cells grown on fibronectin and collagen I produced significantly more superoxide. The oxidative response to cyclic strain was reduced by coincubation with RGD peptides, blocking antibodies to alpha2- and beta-integrins antibodies, as well as inhibitors of protein kinase C. To investigate the effect of oxidative stress on gene transcription, endothelial cells grown on collagen I were transfected with an NFkappaB-sensitive luciferase construct. Cells that underwent cyclic strain displayed a tenfold induction of NFkappaB activation compared to static controls. Strain-induced luciferase activity was blunted by coincubation with RGD peptides or calphostin C. Thus, exposure of endothelial cells to cyclic strain led to integrin activation of a PKC-sensitive pathway that results in increased superoxide anion production and mobilization of NFkappaB.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"12 1","pages":"283-91"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73082234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42