Dairy Science & Technology最新文献

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Effect of Post-Ruminal Casein Infusion on Milk Yield, Milk Composition, and Efficiency of Nitrogen Use in Dairy Cows 瘤胃后灌注酪蛋白对奶牛产奶量、乳成分和氮利用效率的影响
Dairy Science & Technology Pub Date : 2022-02-24 DOI: 10.3390/dairy3010013
M. Ardalan, A. Hussein, E. Titgemeyer
{"title":"Effect of Post-Ruminal Casein Infusion on Milk Yield, Milk Composition, and Efficiency of Nitrogen Use in Dairy Cows","authors":"M. Ardalan, A. Hussein, E. Titgemeyer","doi":"10.3390/dairy3010013","DOIUrl":"https://doi.org/10.3390/dairy3010013","url":null,"abstract":"Adequate supply of amino acids can improve the efficiency of nitrogen use. Casein is the predominant milk protein, and its supplementation can improve milk protein synthesis and nitrogen efficiency. We evaluated the effects of post-ruminal supplementation of casein on milk yield and composition and whole-body protein deposition. Two ruminally cannulated Holstein dairy cows (599 kg) were used in a switch-back design, and treatments were an abomasal infusion of 0 or 400 g/day casein. Cows were fed a diet consisting of corn silage, alfalfa hay, wet corn gluten feed, whole cottonseed, and grain mix, and they received 320 g/day dextrose via abomasal infusion to increase energy:metabolizable protein. The experiment used three 8-day periods. Milk, urine, and feces samples were collected to evaluate milk production, milk composition, and nitrogen retention. Abomasal casein infusion increased (p < 0.01) milk protein percentage and milk urea nitrogen. Nitrogen retention (p = 0.03) and urinary N excretion (p < 0.001) were increased and fecal N excretion (p < 0.001) was decreased by casein infusion. Results suggest casein stimulated protein deposition and altered nitrogen use in lactating dairy cattle. Adaptation periods of 4 days were appropriate for evaluating responses to casein supplementation. Our data provide elements that can aid the design of future experiments.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72808566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of Prebiotics and Synbiotics Carried by Food over Irritable Bowel Syndrome Symptoms: A Systematic Review 食物携带的益生元和合成菌对肠易激综合征症状的影响:系统综述
Dairy Science & Technology Pub Date : 2022-02-17 DOI: 10.3390/dairy3010012
Sofia Steinmetz de Souza, Milena Dutra Pierezan, Guilherme de Souza Hassemer, C. M. Lima, J. De Dea Lindner, Marília Miotto, S. Verruck
{"title":"Effect of Prebiotics and Synbiotics Carried by Food over Irritable Bowel Syndrome Symptoms: A Systematic Review","authors":"Sofia Steinmetz de Souza, Milena Dutra Pierezan, Guilherme de Souza Hassemer, C. M. Lima, J. De Dea Lindner, Marília Miotto, S. Verruck","doi":"10.3390/dairy3010012","DOIUrl":"https://doi.org/10.3390/dairy3010012","url":null,"abstract":"Irritable bowel syndrome (IBS) is a chronic condition that affects 11.2% of the world’s population. The management of gut microbiota using probiotic and synbiotic agents might be a valid alternative to assist in the treatment of IBS. The focus of this study was to evaluate the effects of prebiotic and synbiotic compounds carried by different foods on major symptoms of IBS through a systematic literature review. MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, and LILACS were accessed during July 2021. The studies included in this review were the ones that tested volunteers older than 16 years of age and were conducted using a randomized, controlled clinical trial. The risk of bias was assessed by using the Cochrane risk-of-bias tool for randomized trials (RoB2). Furthermore, the data found were qualitatively evaluated due to the studies’ differences. Two papers were able to fit the criteria, with a total sample size of 280 participants. No datum was found regarding the use of prebiotics in the treatment of IBS. Synbiotic agents, however, had a positive effect on gastrointestinal symptoms and the participants’ overall bowel satisfaction; however, it was not possible to reach a consensus on which effects. Further studies regarding the use of synbiotics and prebiotics must be carried out to determine which effects are the most significant in the treatment of IBS.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79892889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Prevalence of Bovine Norovirus and Nebovirus and Risk Factors of Infection in Swedish Dairy Herds 瑞典奶牛群中诺如病毒和Nebovirus的流行及感染的危险因素
Dairy Science & Technology Pub Date : 2022-02-11 DOI: 10.3390/dairy3010011
M. Tråvén, C. Axén, A. Svensson, C. Björkman, U. Emanuelson
{"title":"Prevalence of Bovine Norovirus and Nebovirus and Risk Factors of Infection in Swedish Dairy Herds","authors":"M. Tråvén, C. Axén, A. Svensson, C. Björkman, U. Emanuelson","doi":"10.3390/dairy3010011","DOIUrl":"https://doi.org/10.3390/dairy3010011","url":null,"abstract":"Enteritis is the major cause of mortality in neonatal calves. Diagnostic work to detect the pathogens involved and identification of risk factors for such infections are imperative to improve calf health. Bovine norovirus (BNoV) and nebovirus prevalence was investigated using RT-PCR in 50 dairy herds in five geographic regions of Sweden. BNoV and nebovirus were found to be common infections in young dairy calves. BNoV was detected in 20% of 250 calves in 48% of the herds. Nebovirus was detected in 5% of the calves in 16% of the herds. Both infections were present in three herds (6%). BNoV-infected calves were younger (median age 7 days) than nebovirus-infected calves (median age 21 days). These infections were not detected in adult cows and young stock over 6 months of age tested in selected herds. Risk factors identified for BNoV infection were a high number of preweaned calves present at the time of sampling, colostrum uptake by suckling the dam only, calf kept in a single pen, and geographic location of herd, while no risk factors for nebovirus infection were identified in the multivariable analysis. Univariable analysis suggested risk factors for nebovirus infection to be geographic location and early separation from the dam.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82554519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Deciphering Molecular Dynamics of Foot and Mouth Disease Virus (FMDV): A Looming Threat to Pakistan’s Dairy Industry 破译口蹄疫病毒(FMDV)的分子动力学:对巴基斯坦奶业迫在眉睫的威胁
Dairy Science & Technology Pub Date : 2022-02-07 DOI: 10.3390/dairy3010010
M. Abubakar, Z. Syed, S. Manzoor, M. Arshed
{"title":"Deciphering Molecular Dynamics of Foot and Mouth Disease Virus (FMDV): A Looming Threat to Pakistan’s Dairy Industry","authors":"M. Abubakar, Z. Syed, S. Manzoor, M. Arshed","doi":"10.3390/dairy3010010","DOIUrl":"https://doi.org/10.3390/dairy3010010","url":null,"abstract":"Milk is seen as a chief source of protein and other biologically available nutrients for human beings. Pakistan, the fourth largest milk-producing country, is badly affected by the contagious transboundary apthoviral disease of ungulate animals; the foot and mouth disease (FMD) virus. FMD is endemic in Pakistan and has caused significant economic loss to the dairy industry in the form of a profound decrease in milk production and increased morbidity and deaths of dairy animals. Inclusively, the case fatality ratio of FMD was 15.11%. Of the seven FMDV serotypes, (O, A, C, Asia 1, SAT 1, SAT2, and SAT 3), three serotypes (O, A, and Asia-1) are endemic in Pakistan. Rapid and highly sensitive diagnostic tools are required for efficient control of this disease. Presently, FMD in the laboratory is diagnosed via ELISA and molecular approaches, i.e., RT-PCR. Serotype-specific RT-PCR analysis not only confirms ELISA serotyping results but can also be used for the screening of ELISA negative samples. Genotypically, FMDV serotype O has a topotype (Middle East–South Asia (ME–SA) and lineage PanAsia-2) that is reported frequently from different areas of Pakistan. Confirmed cases of serotype A and Asia-1 are also reported. The information gathered can be used for understanding the molecular epidemiology of FMD in Pakistan. Further studies on the molecular dynamics of FMD could be useful for ensuring the timely diagnosis of this deadly pathogen, which would ultimately be beneficial for the mass vaccination programs of FMD in Pakistan.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73697459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Assessment of Metabolic Adaptations in Periparturient Dairy Cows Provided 3-Nitrooxypropanol and Varying Concentrate Proportions by Using the GreenFeed System for Indirect Calorimetry, Biochemical Blood Parameters and Ultrasonography of Adipose Tissues 利用GreenFeed系统间接量热法、血液生化指标和脂肪组织超声检查评估3-硝基氧丙醇和不同浓缩比例对围产奶牛代谢适应性的影响
Dairy Science & Technology Pub Date : 2022-02-03 DOI: 10.3390/dairy3010009
Matthias Schilde, D. von Soosten, J. Frahm, S. Kersten, U. Meyer, A. Zeyner, S. Dänicke
{"title":"Assessment of Metabolic Adaptations in Periparturient Dairy Cows Provided 3-Nitrooxypropanol and Varying Concentrate Proportions by Using the GreenFeed System for Indirect Calorimetry, Biochemical Blood Parameters and Ultrasonography of Adipose Tissues","authors":"Matthias Schilde, D. von Soosten, J. Frahm, S. Kersten, U. Meyer, A. Zeyner, S. Dänicke","doi":"10.3390/dairy3010009","DOIUrl":"https://doi.org/10.3390/dairy3010009","url":null,"abstract":"Methanogenesis in ruminants contributes to both greenhouse gas emissions and feed energy losses whereby the latter becomes specifically important in energy-deficient periparturient cows. It was hypothesized that increased concentrate feed proportions (CFP) and feeding with the methane inhibitor 3-nitrooxypropanol (3-NOP), as well as their potential synergism, improve the energy status of peripartal cows. Periparturient dairy cows were fed low or high dietary CFP either tested without or combined with 3-NOP. The GreenFeed system was used to calculate the metabolic respiration quotient (RQmetabolic) and tissue energy retention (ERtissue) by methods of indirect calorimetry. The calorimetrically estimated ERtissue coincided with a conventionally calculated energy balance except for the antepartal period. Neither CFP nor 3-NOP affected the ultrasonographically assessed lipomobilization in adipose depots. In the group fed 3-NOP and a high concentrate feed proportion, the RQmetabolic significantly rose over the course of the experiment and the ERtissue was also increased. Serum non-esterified fatty acid concentrations were lower in the 3-NOP groups albeit ß-hydroxybutyrate (BHB) remained unaffected. Higher CFP reduced BHB and increased blood glucose levels. In conclusion, 3-NOP and high CFP improved the energy budget of the cows in an interactive manner, which was, however, not apparent in all of the examined parameters. The application of the GreenFeed system for indirect calorimetry is a promising approach, which needs further validation in the future.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81980612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acknowledgment to Reviewers of Dairy in 2021 向2021年乳制品审稿人致谢
Dairy Science & Technology Pub Date : 2022-01-29 DOI: 10.3390/dairy3010008
{"title":"Acknowledgment to Reviewers of Dairy in 2021","authors":"","doi":"10.3390/dairy3010008","DOIUrl":"https://doi.org/10.3390/dairy3010008","url":null,"abstract":"Rigorous peer-reviews are the basis of high-quality academic publishing [...]","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74637256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low Birthweight Beef Bulls Compared with Jersey Bulls Do Not Impact First Lactation and Rebreeding of First-Calving Dairy Heifers—A Case Study in New Zealand 与泽西公牛相比,低出生体重的肉牛不会影响初产奶牛的首次泌乳和再繁殖——新西兰的一个案例研究
Dairy Science & Technology Pub Date : 2022-01-27 DOI: 10.3390/dairy3010007
L. Coleman, N. Martín, P. Back, H. Blair, N. López-Villalobos, R. Hickson
{"title":"Low Birthweight Beef Bulls Compared with Jersey Bulls Do Not Impact First Lactation and Rebreeding of First-Calving Dairy Heifers—A Case Study in New Zealand","authors":"L. Coleman, N. Martín, P. Back, H. Blair, N. López-Villalobos, R. Hickson","doi":"10.3390/dairy3010007","DOIUrl":"https://doi.org/10.3390/dairy3010007","url":null,"abstract":"Dairy heifers in New Zealand are typically naturally mated by Jersey bulls to produce low-value non-replacement offspring sold for slaughter a few days after birth. Producing a beef-sired calf from a dairy heifer will increase the value of these calves for beef production but may compromise the subsequent career of the animals, in terms of milk production, rebreeding success, health, or survival of the heifer. This study aimed to determine the impact of low birthweight Angus and Hereford bulls versus breed-average Jersey bulls on the reproduction and production traits of first-calving dairy heifers. The experiment included 304 heifer-calf pairs over 2 years. Calves sired by Angus and Hereford bulls were 3.5–4.4 kg and 3.7–6.8 kg heavier than Jersey-sired calves and had a 4.2% and 9.3% incidence of assistance at birth for normally presented calves over the 2 years, respectively. No normally presented Jersey-sired calves were assisted. There was no difference in body condition score, pre-calving live weight, milk production, pregnancy rate, inter-calving interval, re-calving day, and 21-day re-calving rate of heifers mated to the different breeds of bulls. The results indicate that the Angus and Hereford bulls with low birthweight and high direct calving ease estimated breeding values (EBV) can be used to produce calves of greater value than Jersey-sired calves without impacting dairy heifer production. However, a small increase in assistance at calving could be expected.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90578340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A New Approach in the Evaluation of Dairy Camels: Using Test Day Milk and Morphometric Records 一种评价乳骆驼的新方法:利用试验日乳和形态计量记录
Dairy Science & Technology Pub Date : 2022-01-26 DOI: 10.3390/dairy3010006
Morteza Bitaraf Sani, S. Hosseini, N. Asadzadeh, N. Ghavipanje, Mojtaba Afshin, M. Jasouri, M. H. Banabazi, S. Esmaeilkhanian, Javad Zare Harofte, Ali Shafei Naderi, P. Burger
{"title":"A New Approach in the Evaluation of Dairy Camels: Using Test Day Milk and Morphometric Records","authors":"Morteza Bitaraf Sani, S. Hosseini, N. Asadzadeh, N. Ghavipanje, Mojtaba Afshin, M. Jasouri, M. H. Banabazi, S. Esmaeilkhanian, Javad Zare Harofte, Ali Shafei Naderi, P. Burger","doi":"10.3390/dairy3010006","DOIUrl":"https://doi.org/10.3390/dairy3010006","url":null,"abstract":"In the case of camels, there is little data in the literature on the relationship between body building features and the evaluation of milk yield. In the last decade, a rising interest in camel milk has been observed due to its nutritional and health-promoting properties, resulting in a growing market demand. Despite the remarkable importance of camels, very little improvement in camel breeding and selection for dairy purposes has been achieved. The current study aimed to provide a practical approach to the evaluation of dairy dromedaries based on test day milk and morphometric records. A total of 62 Sindi dairy camels were evaluated and 4176 daily milk records were registered in February, March, April, and May 2021. She-camels were milked twice per day (at morning and evening) by hand before calf sucking. The farming system was intensive with two times feeding. Three measurements had the highest scores in assessing: udder, teats distance and placement, and teats size, which included 45 out of 100 scores. Test day milk records were analysed using a simple repeatability model with two random effects. The range of daily milk yields was estimated between 0.1 to 8.70 kg. The mean of body scores was 77.19 (CI = 74.19–80.19). Daily milk yields moderately correlated with body score (r = +0.27). Additionally, udder circumference and abdomen girth were correlated to milk production. Using test day milk records in breeding programs can be appropriate for the selection and replacement of she-camels, but due to difficulties in accessing these data, using morphometric data is a good criterion for the evaluation of dromedaries in extensive systems.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79206593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Blood Metabolomic Phenotyping of Dry Cows Could Predict the High Milk Somatic Cells in Early Lactation—Preliminary Results 干奶牛血液代谢组学表型预测泌乳早期高乳体细胞的初步结果
Dairy Science & Technology Pub Date : 2022-01-19 DOI: 10.3390/dairy3010005
Klevis Haxhiaj, Zhili Li, Mathew R. Johnson, S. M. Dunn, D. Wishart, B. Ametaj
{"title":"Blood Metabolomic Phenotyping of Dry Cows Could Predict the High Milk Somatic Cells in Early Lactation—Preliminary Results","authors":"Klevis Haxhiaj, Zhili Li, Mathew R. Johnson, S. M. Dunn, D. Wishart, B. Ametaj","doi":"10.3390/dairy3010005","DOIUrl":"https://doi.org/10.3390/dairy3010005","url":null,"abstract":"Subclinical mastitis (SCM) is a very common disease of dairy cows. Currently, somatic cell count (SCC) is used for SCM diagnoses. There are no prognostic tests to detect which cows may develop SCM during the dry-off period. Therefore, the objectives of this study were to identify metabolic alterations in the serum of pre-SCM cows during the dry-off period, at −8 and −4 weeks before calving, through a targeted mass spectrometry (MS) assay. Fifteen cows, free of any disease, and 10 cows affected only by SCM postpartum served as controls (CON) and the SCM group, respectively. Results showed 59 and 47 metabolites that differentiated (p ≤ 0.05) CON and pre-SCM cows at –8 and −4 weeks prior to the expected date of parturition, respectively. Regression analysis indicated that a panel of four serum metabolites (AUC = 0.92, p < 0.001) at −8 weeks and another four metabolites (AUC = 0.92, p < 0.01) at −4 weeks prior to parturition might serve as predictive biomarkers for SCM. Early identification of susceptible cows can enable development of better preventive measurements ahead of disease occurrence.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86646986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
First Insight into the Variation of the Milk Serum Proteome within and between Individual Cows 首次洞察牛奶血清蛋白质组在个体奶牛内部和之间的变化
Dairy Science & Technology Pub Date : 2022-01-14 DOI: 10.3390/dairy3010004
Lina Zhang, S. Boeren, J. Heck, J. Vervoort, Peng Zhou, K. Hettinga
{"title":"First Insight into the Variation of the Milk Serum Proteome within and between Individual Cows","authors":"Lina Zhang, S. Boeren, J. Heck, J. Vervoort, Peng Zhou, K. Hettinga","doi":"10.3390/dairy3010004","DOIUrl":"https://doi.org/10.3390/dairy3010004","url":null,"abstract":"Milk contains all nutrients needed for development of calves. One important group of components responsible for this are the milk proteins. Variation due to feed or animal health, has been studied for the most abundant milk proteins. The aim of this study was to determine the variation between and within cows for their milk serum proteome. Sample Set 1 was collected from Holstein Friesian (HF) cows between November 2011 and March 2012 and prepared using filter aided sample preparation (FASP) followed by LC-MS/MS for protein identification and quantification. The results showed that the milk serum proteome was very constant in mid lactation (four cows at five time points, p > 0.05) between 3 and 6 months in lactation. Sample Set 2 was collected from HF cows in Dec 2012 and analyzed using FASP and dimethyl labeling followed by LC-MS/MS. Significant variation in the milk serum proteome (p < 0.05) between 17 individual cows was found in Sample Set 2. The most variable proteins were immune-related proteins, which may reflect the health status of the individual cow. On the other hand, proteins related to nutrient synthesis and transport were relatively constant, indicating the importance of milk in providing a stable supply of nutrients to the neonate. In conclusion, the milk serum proteome was stable over mid lactation, but differed significantly between individuals, especially in immune-related proteins.","PeriodicalId":11001,"journal":{"name":"Dairy Science & Technology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80539402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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